{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T17:45:29Z","timestamp":1793295929615,"version":"4.5.0"},"reference-count":41,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T00:00:00Z","timestamp":1680739200000},"content-version":"am","delay-in-days":309,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100006975","name":"Indiana Clinical and Translational Sciences Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006975","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010249","name":"School of Medicine, Indiana University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010249","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R01EY026962"],"award-info":[{"award-number":["R01EY026962"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R01EY031700"],"award-info":[{"award-number":["R01EY031700"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001818","name":"Research to Prevent Blindness","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006312","name":"BrightFocus Foundation","doi-asserted-by":"publisher","award":["G2017151"],"award-info":[{"award-number":["G2017151"]}],"id":[{"id":"10.13039\/100006312","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["UL1TR002529"],"award-info":[{"award-number":["UL1TR002529"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Eye Research"],"published-print":{"date-parts":[[2022,6]]},"DOI":"10.1016\/j.exer.2022.109055","type":"journal-article","created":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T20:57:17Z","timestamp":1648760237000},"page":"109055","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Age and sex affect TGF\u03b22-induced ocular hypertension in C57BL\/6J mice"],"prefix":"10.1016","volume":"219","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6523-7688","authenticated-orcid":false,"given":"Chenna Kesavulu","family":"Sugali","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9973-1553","authenticated-orcid":false,"given":"Naga Pradeep","family":"Rayana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0514-4890","authenticated-orcid":false,"given":"Jiannong","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0597-1490","authenticated-orcid":false,"given":"Weiming","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.exer.2022.109055_bib1","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1016\/S0161-6420(84)34248-8","article-title":"Trabecular meshwork cellularity in primary open-angle glaucoma and nonglaucomatous normals","volume":"91","author":"Alvarado","year":"1984","journal-title":"Ophthalmology"},{"key":"10.1016\/j.exer.2022.109055_bib2","first-page":"281","article-title":"Glucocorticoid-induced formation of cross-linked actin networks in cultured human trabecular meshwork cells","volume":"35","author":"Clark","year":"1994","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2022.109055_bib3","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1001\/archopht.123.2.220","article-title":"Plasminogen activator inhibitor-1 in the aqueous humor of patients with and without glaucoma","volume":"123","author":"Dan","year":"2005","journal-title":"Arch. Ophthalmol."},{"key":"10.1016\/j.exer.2022.109055_bib4","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1016\/j.exer.2011.03.016","article-title":"Effect of general anesthetics on IOP in elevated IOP mouse model","volume":"92","author":"Ding","year":"2011","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2022.109055_bib5","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0237932","article-title":"Disruption of fibronectin fibrillogenesis affects intraocular pressure (IOP) in BALB\/cJ mice","volume":"15","author":"Faralli","year":"2020","journal-title":"PLoS One"},{"key":"10.1016\/j.exer.2022.109055_bib6","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.exer.2016.05.011","article-title":"The role of integrins in glaucoma","volume":"158","author":"Filla","year":"2017","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2022.109055_bib7","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1167\/iovs.05-1060","article-title":"TGF\u03b22-Induced changes in human trabecular meshwork: implications for intraocular pressure","volume":"47","author":"Fleenor","year":"2006","journal-title":"Invest. ophthamol. Visual Sci."},{"key":"10.1016\/j.exer.2022.109055_bib8","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1167\/iovs.03-0796","article-title":"Effects of TGF-beta2 in perfused human eyes","volume":"45","author":"Gottanka","year":"2004","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2022.109055_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.exer.2020.107920","article-title":"Nuclear factor-kappa beta signaling is required for transforming growth factor Beta-2 induced ocular hypertension","volume":"191","author":"Hernandez","year":"2020","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2022.109055_bib10","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1167\/iovs.08-2706","article-title":"Cross-linked actin networks (CLANs) in the trabecular meshwork of the normal and glaucomatous human eye in situ","volume":"50","author":"Hoare","year":"2009","journal-title":"Invest. ophthamol. Visual Sci."},{"key":"10.1016\/j.exer.2022.109055_bib11","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/s004170000241","article-title":"Transforming growth factor-beta 2 levels in aqueous humor of glaucomatous eyes","volume":"239","author":"Inatani","year":"2001","journal-title":"Graefes Arch. Clin. Exp. Ophthalmol."},{"key":"10.1016\/j.exer.2022.109055_bib12","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/S0002-9394(00)00538-9","article-title":"The Advanced Glaucoma Intervention Study (AGIS): 7. The relationship between control of intraocular pressure and visual field deterioration.The AGIS Investigators","volume":"130","author":"Investigators","year":"2000","journal-title":"Am. J. Ophthalmol."},{"key":"10.1016\/j.exer.2022.109055_bib13","doi-asserted-by":"crossref","first-page":"14951","DOI":"10.1038\/s41598-017-14938-0","article-title":"Increased synthesis and deposition of extracellular matrix proteins leads to endoplasmic reticulum stress in the trabecular meshwork","volume":"7","author":"Kasetti","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.exer.2022.109055_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.exer.2021.108494","article-title":"Circumferential trabecular meshwork cell density in the human eye","volume":"205","author":"Kuehn","year":"2021","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2022.109055_bib15","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/0014-4835(89)90027-4","article-title":"Immunomicroscopical study of type VI collagen in the trabecular meshwork of normal and glaucomatous eyes","volume":"48","author":"L\u00fctjen-Drecoll","year":"1989","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2022.109055_bib16","first-page":"471","article-title":"[Novel therapeutic targets for reduction of intraocular pressure in primary open angle glaucoma]","volume":"52","author":"Mao","year":"2016","journal-title":"Zhonghua Yan Ke Za Zhi"},{"key":"10.1016\/j.exer.2022.109055_bib17","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1055\/s-0037-1613457","article-title":"Aging of stromal-derived human breast fibroblasts might contribute to breast cancer progression","volume":"89","author":"Martens","year":"2003","journal-title":"Thromb. Haemostasis"},{"key":"10.1016\/j.exer.2022.109055_bib18","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.exer.2012.04.016","article-title":"Mutant human myocilin induces strain specific differences in ocular hypertension and optic nerve damage in mice","volume":"100","author":"McDowell","year":"2012","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2022.109055_bib19","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.exer.2013.10.017","article-title":"Smad3 is necessary for transforming growth factor-beta2 induced ocular hypertension in mice","volume":"116","author":"McDowell","year":"2013","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2022.109055_bib20","doi-asserted-by":"crossref","first-page":"6779","DOI":"10.1167\/iovs.13-12298","article-title":"Cellular fibronectin expression in human trabecular meshwork and induction by transforming growth factor-\u03b22","volume":"54","author":"Medina-Ortiz","year":"2013","journal-title":"Invest. ophthamol. Visual Sci."},{"key":"10.1016\/j.exer.2022.109055_bib21","first-page":"10","article-title":"Effect of immunomodulation with anti-CD40L antibody on adenoviral-mediated transgene expression in mouse anterior segment","volume":"14","author":"Millar","year":"2008","journal-title":"Mol. Vis."},{"key":"10.1016\/j.exer.2022.109055_bib22","doi-asserted-by":"crossref","DOI":"10.1016\/j.exer.2020.108241","article-title":"Evidence that cannabinoid CB1 receptors regulate intraocular pressure via two opposing mechanisms","volume":"200","author":"Miller","year":"2020","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2022.109055_bib23","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1167\/iovs.16-19672","article-title":"TGF\u03b22 induces the formation of cross-linked actin networks (CLANs) in human trabecular meshwork cells through the Smad and non-smad dependent pathways","volume":"58","author":"Montecchi-Palmer","year":"2017","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2022.109055_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.preteyeres.2019.100799","article-title":"Inducible rodent models of glaucoma","volume":"75","author":"Pang","year":"2020","journal-title":"Prog. Retin. Eye Res."},{"key":"10.1016\/j.exer.2022.109055_bib25","doi-asserted-by":"crossref","first-page":"4447","DOI":"10.1167\/iovs.15-16739","article-title":"Dexamethasone stiffens trabecular meshwork, trabecular meshwork cells, and matrix","volume":"56","author":"Raghunathan","year":"2015","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2022.109055_bib26","doi-asserted-by":"crossref","first-page":"4447","DOI":"10.1167\/iovs.15-16739","article-title":"Dexamethasone stiffens trabecular meshwork, trabecular meshwork cells, and matrix","volume":"56","author":"Raghunathan","year":"2015","journal-title":"Invest. ophthamol. Visual Sci."},{"key":"10.1016\/j.exer.2022.109055_bib27","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1167\/iovs.62.12.7","article-title":"Using CRISPR interference as a therapeutic approach to treat TGFbeta2-induced ocular hypertension and glaucoma","volume":"62","author":"Rayana","year":"2021","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2022.109055_bib28","first-page":"844","article-title":"Global data on visual impairment in the year 2002","volume":"82","author":"Resnikoff","year":"2004","journal-title":"Bull. World Health Organ."},{"key":"10.1016\/j.exer.2022.109055_bib29","doi-asserted-by":"crossref","first-page":"9815","DOI":"10.1038\/s41598-020-66756-6","article-title":"Fibronectin extra domain A (FN-EDA) elevates intraocular pressure through Toll-like receptor 4 signaling","volume":"10","author":"Roberts","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.exer.2022.109055_bib30","doi-asserted-by":"crossref","first-page":"5240","DOI":"10.1167\/iovs.11-7287","article-title":"Transforming growth factor-beta induces extracellular matrix protein cross-linking lysyl oxidase (LOX) genes in human trabecular meshwork cells","volume":"52","author":"Sethi","year":"2011","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2022.109055_bib31","doi-asserted-by":"crossref","first-page":"2067","DOI":"10.1167\/iovs.09-4567","article-title":"Adenoviral gene transfer of active human transforming growth factor-{beta}2 elevates intraocular pressure and reduces outflow facility in rodent eyes","volume":"51","author":"Shepard","year":"2010","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2022.109055_bib32","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/j.exer.2011.06.011","article-title":"Transforming growth factor-beta2 utilizes the canonical Smad-signaling pathway to regulate tissue transglutaminase expression in human trabecular meshwork cells","volume":"93","author":"Tovar-Vidales","year":"2011","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2022.109055_bib33","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1167\/iovs.07-0835","article-title":"Tissue transglutaminase expression and activity in normal and glaucomatous human trabecular meshwork cells and tissues","volume":"49","author":"Tovar-Vidales","year":"2008","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2022.109055_bib34","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1006\/exer.1994.1158","article-title":"Aqueous humor in glaucomatous eyes contains an increased level of TGF-beta 2","volume":"59","author":"Tripathi","year":"1994","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2022.109055_bib35","doi-asserted-by":"crossref","DOI":"10.1016\/j.exer.2020.108420","article-title":"Effect of inhalation anesthesia with isoflurane on circadian rhythm of murine intraocular pressure","volume":"203","author":"Tsuchiya","year":"2021","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2022.109055_bib36","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.exer.2014.07.014","article-title":"Extracellular matrix in the trabecular meshwork: intraocular pressure regulation and dysregulation in glaucoma","volume":"133","author":"Vranka","year":"2015","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2022.109055_bib37","doi-asserted-by":"crossref","first-page":"5848","DOI":"10.1038\/s41598-018-24165-w","article-title":"The relationship between outflow resistance and trabecular meshwork stiffness in mice","volume":"8","author":"Wang","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.exer.2022.109055_bib38","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.exer.2016.07.011","article-title":"Trabecular meshwork stiffness in glaucoma","volume":"158","author":"Wang","year":"2017","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2022.109055_bib39","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1089\/jop.2013.0220","article-title":"The role of TGF-beta2 and bone morphogenetic proteins in the trabecular meshwork and glaucoma","volume":"30","author":"Wordinger","year":"2014","journal-title":"J. Ocul. Pharmacol. Therapeut."},{"key":"10.1016\/j.exer.2022.109055_bib40","doi-asserted-by":"crossref","first-page":"4023","DOI":"10.1167\/iovs.04-0535","article-title":"Gene and protein expression changes in human trabecular meshwork cells treated with transforming growth factor-beta","volume":"45","author":"Zhao","year":"2004","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2022.109055_bib41","doi-asserted-by":"crossref","first-page":"1956","DOI":"10.1172\/JCI69774","article-title":"Ocular-specific ER stress reduction rescues glaucoma in murine glucocorticoid-induced glaucoma","volume":"124","author":"Zode","year":"2014","journal-title":"J. Clin. Invest."}],"container-title":["Experimental Eye Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S001448352200135X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S001448352200135X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T05:33:24Z","timestamp":1762148004000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S001448352200135X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6]]},"references-count":41,"alternative-id":["S001448352200135X"],"URL":"https:\/\/doi.org\/10.1016\/j.exer.2022.109055","relation":{},"ISSN":["0014-4835"],"issn-type":[{"value":"0014-4835","type":"print"}],"subject":[],"published":{"date-parts":[[2022,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Age and sex affect TGF\u03b22-induced ocular hypertension in C57BL\/6J mice","name":"articletitle","label":"Article Title"},{"value":"Experimental Eye Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exer.2022.109055","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"109055"}}