{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T17:20:34Z","timestamp":1777396834838,"version":"3.51.4"},"reference-count":21,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,6,18]],"date-time":"2023-06-18T00:00:00Z","timestamp":1687046400000},"content-version":"am","delay-in-days":321,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100006312","name":"BrightFocus Foundation","doi-asserted-by":"publisher","award":["G2017151"],"award-info":[{"award-number":["G2017151"]}],"id":[{"id":"10.13039\/100006312","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006975","name":"Indiana Clinical and Translational Sciences Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006975","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010249","name":"School of Medicine, Indiana University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010249","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R01EY026962"],"award-info":[{"award-number":["R01EY026962"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R01EY031700"],"award-info":[{"award-number":["R01EY031700"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["T35EY031282"],"award-info":[{"award-number":["T35EY031282"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["UL1TR002529"],"award-info":[{"award-number":["UL1TR002529"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001818","name":"Research to Prevent Blindness","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001818","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Eye Research"],"published-print":{"date-parts":[[2022,8]]},"DOI":"10.1016\/j.exer.2022.109137","type":"journal-article","created":{"date-parts":[[2022,6,9]],"date-time":"2022-06-09T16:36:31Z","timestamp":1654792591000},"page":"109137","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["The application of lentiviral vectors for the establishment of TGF\u03b22-induced ocular hypertension in C57BL\/6J mice"],"prefix":"10.1016","volume":"221","author":[{"given":"Michael","family":"Peng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tyler J.","family":"Margetts","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9973-1553","authenticated-orcid":false,"given":"Naga Pradeep","family":"Rayana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenna Kesavulu","family":"Sugali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiannong","family":"Dai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0597-1490","authenticated-orcid":false,"given":"Weiming","family":"Mao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.exer.2022.109137_bib1","doi-asserted-by":"crossref","first-page":"257","DOI":"10.4103\/0301-4738.53049","article-title":"Current concepts in the pathophysiology of glaucoma","volume":"57","author":"Agarwal","year":"2009","journal-title":"Indian J. Ophthalmol."},{"key":"10.1016\/j.exer.2022.109137_bib2","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1038\/gt.2011.153","article-title":"Ocular gene delivery using lentiviral vectors","volume":"19","author":"Balaggan","year":"2012","journal-title":"Gene Ther."},{"key":"10.1016\/j.exer.2022.109137_bib3","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1089\/hum.2018.115","article-title":"Sustained and widespread gene delivery to the corneal epithelium via in situ transduction of limbal epithelial stem cells, using lentiviral and adeno-associated viral vectors","volume":"29","author":"Basche","year":"2018","journal-title":"Hum. Gene Ther."},{"key":"10.1016\/j.exer.2022.109137_bib4","doi-asserted-by":"crossref","first-page":"2506","DOI":"10.1167\/iovs.12-9483o","article-title":"Advances in glaucoma treatment and management: gene therapy","volume":"53","author":"Borras","year":"2012","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2022.109137_bib5","doi-asserted-by":"crossref","first-page":"194","DOI":"10.2174\/1566523217666171116170040","article-title":"The role of gene therapy in the treatment of retinal diseases: a review","volume":"17","author":"Campa","year":"2017","journal-title":"Curr. Gene Ther."},{"key":"10.1016\/j.exer.2022.109137_bib6","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1007\/s00347-002-0770-2","article-title":"[Effect of transforming growth factor beta 2 on phagocytosis in cultured bovine trabecular meshwork cells]","volume":"100","author":"Cao","year":"2003","journal-title":"Ophthalmologe"},{"key":"10.1016\/j.exer.2022.109137_bib7","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1167\/iovs.05-1060","article-title":"TGF\u03b22-Induced changes in human trabecular meshwork: implications for intraocular pressure","volume":"47","author":"Fleenor","year":"2006","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2022.109137_bib8","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1167\/iovs.05-1060","article-title":"TGFbeta2-induced changes in human trabecular meshwork: implications for intraocular pressure","volume":"47","author":"Fleenor","year":"2006","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2022.109137_bib9","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1167\/iovs.03-0796","article-title":"Effects of TGF-beta2 in perfused human eyes","volume":"45","author":"Gottanka","year":"2004","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2022.109137_bib10","doi-asserted-by":"crossref","first-page":"1811","DOI":"10.1167\/iovs.16-21331","article-title":"Crosstalk between transforming growth factor beta-2 and toll-like receptor 4 in the trabecular meshwork","volume":"58","author":"Hernandez","year":"2017","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2022.109137_bib11","doi-asserted-by":"crossref","first-page":"235","DOI":"10.2174\/1566523217666171003170348","article-title":"Comparative study of adeno-associated virus, adenovirus, Bacu lovirus and lentivirus vectors for gene therapy of the eyes","volume":"17","author":"Kalesnykas","year":"2017","journal-title":"Curr. Gene Ther."},{"key":"10.1016\/j.exer.2022.109137_bib12","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.exer.2011.07.001","article-title":"Experimental gene transfer to the corneal endothelium","volume":"95","author":"Kampik","year":"2012","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2022.109137_bib13","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1056\/NEJMra0804630","article-title":"Primary open-angle glaucoma","volume":"360","author":"Kwon","year":"2009","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.exer.2022.109137_bib14","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.gendis.2017.04.001","article-title":"Adenovirus-mediated gene delivery: potential applications for gene and cell-based therapies in the new era of personalized medicine","volume":"4","author":"Lee","year":"2017","journal-title":"Genes Dis."},{"key":"10.1016\/j.exer.2022.109137_bib15","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1089\/hum.2010.132","article-title":"Pigment epithelium-derived factor gene therapy targeting retinal ganglion cell injuries: neuroprotection against loss of function in two animal models","volume":"22","author":"Miyazaki","year":"2011","journal-title":"Hum. Gene Ther."},{"key":"10.1016\/j.exer.2022.109137_bib16","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1167\/iovs.16-19672","article-title":"TGF\u03b22 induces the formation of cross-linked actin networks (CLANs) in human trabecular meshwork cells through the smad and non-smad dependent pathways","volume":"58","author":"Montecchi-Palmer","year":"2017","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2022.109137_bib17","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.exer.2015.04.003","article-title":"Elevation of intraocular pressure in rodents using viral vectors targeting the trabecular meshwork","volume":"141","author":"Pang","year":"2015","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2022.109137_bib18","doi-asserted-by":"crossref","first-page":"2067","DOI":"10.1167\/iovs.09-4567","article-title":"Adenoviral gene transfer of active human transforming growth factor-{beta}2 elevates intraocular pressure and reduces outflow facility in rodent eyes","volume":"51","author":"Shepard","year":"2010","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2022.109137_bib19","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/j.exer.2011.06.011","article-title":"Transforming growth factor-beta2 utilizes the canonical Smad-signaling pathway to regulate tissue transglutaminase expression in human trabecular meshwork cells","volume":"93","author":"Tovar-Vidales","year":"2011","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2022.109137_bib20","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1006\/exer.1994.1158","article-title":"Aqueous humor in glaucomatous eyes contains an increased level of TGF-beta 2","volume":"59","author":"Tripathi","year":"1994","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2022.109137_bib21","first-page":"1575","article-title":"Cultured human trabecular meshwork cells express functional growth factor receptors","volume":"39","author":"Wordinger","year":"1998","journal-title":"Invest. Ophthalmol. Vis. Sci."}],"container-title":["Experimental Eye Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483522002172?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483522002172?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T05:33:30Z","timestamp":1762148010000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014483522002172"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8]]},"references-count":21,"alternative-id":["S0014483522002172"],"URL":"https:\/\/doi.org\/10.1016\/j.exer.2022.109137","relation":{},"ISSN":["0014-4835"],"issn-type":[{"value":"0014-4835","type":"print"}],"subject":[],"published":{"date-parts":[[2022,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The application of lentiviral vectors for the establishment of TGF\u03b22-induced ocular hypertension in C57BL\/6J mice","name":"articletitle","label":"Article Title"},{"value":"Experimental Eye Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exer.2022.109137","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"109137"}}