{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:25:34Z","timestamp":1792625134611,"version":"4.5.0"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82060173"],"award-info":[{"award-number":["82060173"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82101084"],"award-info":[{"award-number":["82101084"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2021M69898"],"award-info":[{"award-number":["2021M69898"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100019533","name":"Science Fund for Distinguished Young Scholars of Fujian Province","doi-asserted-by":"publisher","award":["2023J06053"],"award-info":[{"award-number":["2023J06053"]}],"id":[{"id":"10.13039\/501100019533","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Eye Research"],"published-print":{"date-parts":[[2023,8]]},"DOI":"10.1016\/j.exer.2023.109544","type":"journal-article","created":{"date-parts":[[2023,6,17]],"date-time":"2023-06-17T10:53:56Z","timestamp":1686999236000},"page":"109544","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"special_numbering":"C","title":["Optimization of method for achieving a single-cell suspension from mouse corneas"],"prefix":"10.1016","volume":"233","author":[{"given":"Xiaodong","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengpeng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sijie","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huping","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9226-5887","authenticated-orcid":false,"given":"Shangkun","family":"Ou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"4","key":"10.1016\/j.exer.2023.109544_bib1","doi-asserted-by":"crossref","first-page":"380","DOI":"10.23736\/S0393-2249.18.03118-1","article-title":"Collagenase clostridium histolyticum: a novel medical treatment for Peyronie's disease","volume":"70","author":"Abdel Raheem","year":"2018","journal-title":"Minerva Urol. Nefrol."},{"issue":"2","key":"10.1016\/j.exer.2023.109544_bib2","doi-asserted-by":"crossref","first-page":"163","DOI":"10.3109\/07388551.2015.1128876","article-title":"Flow cytometry: basic principles and applications","volume":"37","author":"Adan","year":"2017","journal-title":"Crit. Rev. Biotechnol."},{"issue":"7","key":"10.1016\/j.exer.2023.109544_bib3","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1016\/j.stem.2021.04.003","article-title":"Discrete limbal epithelial stem cell populations mediate corneal homeostasis and wound healing","volume":"28","author":"Altshuler","year":"2021","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.exer.2023.109544_bib4","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.jtos.2021.03.010","article-title":"A single cell atlas of human cornea that defines its development, limbal progenitor cells and their interactions with the immune cells","volume":"21","author":"Collin","year":"2021","journal-title":"Ocul. Surf."},{"key":"10.1016\/j.exer.2023.109544_bib5","doi-asserted-by":"crossref","DOI":"10.1038\/sdata.2018.13","article-title":"Single cell RNA sequencing of stem cell-derived retinal ganglion cells","volume":"5","author":"Daniszewski","year":"2018","journal-title":"Sci. Data"},{"key":"10.1016\/j.exer.2023.109544_bib6","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.jtos.2021.04.010","article-title":"Molecular identity of human limbal heterogeneity involved in corneal homeostasis and privilege","volume":"21","author":"Dou","year":"2021","journal-title":"Ocul. Surf."},{"key":"10.1016\/j.exer.2023.109544_bib7","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/bs.pmbts.2015.04.001","article-title":"Overview of the cornea: structure, function, and development","volume":"134","author":"Eghrari","year":"2015","journal-title":"Prog Mol Biol Transl Sci"},{"key":"10.1016\/j.exer.2023.109544_bib8","doi-asserted-by":"crossref","DOI":"10.1016\/j.exer.2020.108137","article-title":"Composition, structure and function of the corneal stroma","volume":"198","author":"Espana","year":"2020","journal-title":"Exp. Eye Res."},{"issue":"2\u20133","key":"10.1016\/j.exer.2023.109544_bib9","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.mam.2005.12.003","article-title":"RNA integrity and the effect on the real-time qRT-PCR performance","volume":"27","author":"Fleige","year":"2006","journal-title":"Mol. Aspect. Med."},{"key":"10.1016\/j.exer.2023.109544_bib10","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.mam.2017.07.003","article-title":"Single-cell RNA sequencing: technical advancements and biological applications","volume":"59","author":"Hedlund","year":"2018","journal-title":"Mol. Aspect. Med."},{"key":"10.1016\/j.exer.2023.109544_bib11","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/978-1-4939-3628-1_4","article-title":"Isolation and culture of human endothelial cells from micro- and macro-vessels","volume":"1430","author":"Hewett","year":"2016","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.exer.2023.109544_bib12","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/978-1-62703-628-3_4","article-title":"Isolation and culture of rabbit embryonic stem cells","volume":"1074","author":"Honda","year":"2013","journal-title":"Methods Mol. Biol."},{"issue":"8","key":"10.1016\/j.exer.2023.109544_bib13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s12276-018-0071-8","article-title":"Single-cell RNA sequencing technologies and bioinformatics pipelines","volume":"50","author":"Hwang","year":"2018","journal-title":"Exp. Mol. Med."},{"issue":"10","key":"10.1016\/j.exer.2023.109544_bib14","doi-asserted-by":"crossref","first-page":"3570","DOI":"10.1167\/iovs.19-27656","article-title":"Single-cell RNA transcriptome helps define the limbal\/corneal epithelial stem\/early transit amplifying cells and how autophagy affects this population","volume":"60","author":"Kaplan","year":"2019","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2023.109544_bib15","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.exer.2014.06.002","article-title":"N-Acetylcysteine increases corneal endothelial cell survival in a mouse model of Fuchs endothelial corneal dystrophy","volume":"127","author":"Kim","year":"2014","journal-title":"Exp. Eye Res."},{"issue":"1","key":"10.1016\/j.exer.2023.109544_bib16","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-94933-8","article-title":"Molecular characteristics and spatial distribution of adult human corneal cell subtypes","volume":"11","author":"Ligocki","year":"2021","journal-title":"Sci. Rep."},{"issue":"4","key":"10.1016\/j.exer.2023.109544_bib17","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1080\/08820538.2021.1889615","article-title":"Single-cell RNA sequencing: an overview for the ophthalmologist","volume":"36","author":"Rossin","year":"2021","journal-title":"Semin. Ophthalmol."},{"issue":"2","key":"10.1016\/j.exer.2023.109544_bib18","doi-asserted-by":"crossref","DOI":"10.3390\/ph9020033","article-title":"Tumor heterogeneity, single-cell sequencing, and drug resistance","volume":"9","author":"Schmidt","year":"2016","journal-title":"Pharmaceuticals"},{"issue":"1","key":"10.1016\/j.exer.2023.109544_bib19","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s12325-017-0648-y","article-title":"The role of trypsin:chymotrypsin in tissue repair","volume":"35","author":"Shah","year":"2018","journal-title":"Adv. Ther."},{"issue":"3","key":"10.1016\/j.exer.2023.109544_bib20","doi-asserted-by":"crossref","first-page":"487","DOI":"10.2174\/0929867324666171006124236","article-title":"Collagenolytic enzymes and their applications in biomedicine","volume":"26","author":"Shekhter","year":"2019","journal-title":"Curr. Med. Chem."},{"issue":"6","key":"10.1016\/j.exer.2023.109544_bib21","doi-asserted-by":"crossref","first-page":"3626","DOI":"10.1167\/iovs.13-12955","article-title":"Effects of the loss of conjunctival Muc16 on corneal epithelium and stroma in mice","volume":"55","author":"Shirai","year":"2014","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2023.109544_bib22","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1007\/978-1-0716-1307-8_19","article-title":"Single-cell RNA sequencing analysis: a step-by-step overview","volume":"2284","author":"Slovin","year":"2021","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.exer.2023.109544_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.exer.2021.108767","article-title":"Comparison of different methods to isolate mouse limbal epithelial cells","volume":"212","author":"Song","year":"2021","journal-title":"Exp. Eye Res."},{"issue":"6","key":"10.1016\/j.exer.2023.109544_bib24","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1111\/exd.13368","article-title":"Methods for the isolation and 3D culture of dermal papilla cells from human hair follicles","volume":"26","author":"Topouzi","year":"2017","journal-title":"Exp. Dermatol."},{"issue":"3","key":"10.1016\/j.exer.2023.109544_bib25","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0194820","article-title":"The enzymatic de-epithelialization technique determines denuded amniotic membrane integrity and viability of harvested epithelial cells","volume":"13","author":"Trosan","year":"2018","journal-title":"PLoS One"},{"key":"10.1016\/j.exer.2023.109544_bib26","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/s12859-016-0922-z","article-title":"Measure transcript integrity using RNA-seq data","volume":"17","author":"Wang","year":"2016","journal-title":"BMC Bioinf."},{"issue":"6","key":"10.1016\/j.exer.2023.109544_bib27","doi-asserted-by":"crossref","DOI":"10.15252\/embj.2020105123","article-title":"Mapping the origin and fate of myeloid cells in distinct compartments of the eye by single-cell profiling","volume":"40","author":"Wieghofer","year":"2021","journal-title":"EMBO J."},{"issue":"4","key":"10.1016\/j.exer.2023.109544_bib28","doi-asserted-by":"crossref","first-page":"3072","DOI":"10.1167\/iovs.12-10805","article-title":"The effect of amniotic membrane de-epithelialization method on its biological properties and ability to promote limbal epithelial cell culture","volume":"54","author":"Zhang","year":"2013","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"issue":"7","key":"10.1016\/j.exer.2023.109544_bib29","doi-asserted-by":"crossref","DOI":"10.3390\/ijms18071398","article-title":"Dry eye management: targeting the ocular surface microenvironment","volume":"18","author":"Zhang","year":"2017","journal-title":"Int. J. Mol. Sci."}],"container-title":["Experimental Eye Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483523001653?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483523001653?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T05:37:07Z","timestamp":1762148227000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014483523001653"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8]]},"references-count":29,"alternative-id":["S0014483523001653"],"URL":"https:\/\/doi.org\/10.1016\/j.exer.2023.109544","relation":{},"ISSN":["0014-4835"],"issn-type":[{"value":"0014-4835","type":"print"}],"subject":[],"published":{"date-parts":[[2023,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Optimization of method for achieving a single-cell suspension from mouse corneas","name":"articletitle","label":"Article Title"},{"value":"Experimental Eye Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exer.2023.109544","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"109544"}}