{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:33:57Z","timestamp":1790627637019,"version":"4.1.0"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,7,20]],"date-time":"2024-07-20T00:00:00Z","timestamp":1721433600000},"content-version":"am","delay-in-days":323,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["EY14801"],"award-info":[{"award-number":["EY14801"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30EY031631"],"award-info":[{"award-number":["P30EY031631"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01EY032960"],"award-info":[{"award-number":["R01EY032960"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21EY033961"],"award-info":[{"award-number":["R21EY033961"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["EY031292"],"award-info":[{"award-number":["EY031292"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001818","name":"Research to Prevent Blindness","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006686","name":"University of Miami","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006686","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Eye Research"],"published-print":{"date-parts":[[2023,9]]},"DOI":"10.1016\/j.exer.2023.109562","type":"journal-article","created":{"date-parts":[[2023,6,27]],"date-time":"2023-06-27T20:36:28Z","timestamp":1687898188000},"page":"109562","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"special_numbering":"C","title":["Glaucomatous aqueous humor vesicles are smaller and differ in composition compared to controls"],"prefix":"10.1016","volume":"234","author":[{"given":"Anna","family":"Mueller","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdelrahman","family":"Anter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genea","family":"Edwards","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna K.","family":"Junk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noel","family":"Ziebarth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3759-647X","authenticated-orcid":false,"given":"Sanjoy K.","family":"Bhattacharya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.exer.2023.109562_bib1","article-title":"The trabecular meshwork: a basic review of form and function","volume":"2","author":"Abu-Hassan","year":"2014","journal-title":"J. Ocul. Biol."},{"key":"10.1016\/j.exer.2023.109562_bib2","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.exer.2008.01.013","article-title":"Extracellular matrix in the trabecular meshwork","volume":"86","author":"Acott","year":"2008","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2023.109562_bib3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11060-013-1084-8","article-title":"Biogenesis of extracellular vesicles (EV): exosomes, microvesicles, retrovirus-like vesicles, and apoptotic bodies","volume":"113","author":"Akers","year":"2013","journal-title":"J. Neuro Oncol."},{"key":"10.1016\/j.exer.2023.109562_bib4","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.3109\/02713683.2013.803123","article-title":"Cholesterol and glycosphingolipids of human trabecular meshwork and aqueous humor: comparative profiles from control and glaucomatous donors","volume":"38","author":"Aribindi","year":"2013","journal-title":"Curr. Eye Res."},{"key":"10.1016\/j.exer.2023.109562_bib5","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1016\/j.exer.2005.01.028","article-title":"Cochlin deposits in the trabecular meshwork of the glaucomatous DBA\/2J mouse","volume":"80","author":"Bhattacharya","year":"2005","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2023.109562_bib6","doi-asserted-by":"crossref","first-page":"5984","DOI":"10.1167\/iovs.16-19734","article-title":"Aberrant collagen composition of the trabecular meshwork results in reduced aqueous humor drainage and elevated IOP in MMP-9 null mice","volume":"57","author":"De Groef","year":"2016","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2023.109562_bib7","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.exer.2015.01.019","article-title":"Human aqueous humor exosomes","volume":"132","author":"Dismuke","year":"2015","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2023.109562_bib8","doi-asserted-by":"crossref","DOI":"10.1080\/20013078.2017.1302705","article-title":"Characterisation of extracellular vesicle-subsets derived from brain endothelial cells and analysis of their protein cargo modulation after TNF exposure","volume":"6","author":"Dozio","year":"2017","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.exer.2023.109562_bib9","doi-asserted-by":"crossref","first-page":"3856","DOI":"10.1111\/jcmm.14975","article-title":"Endogenous ocular lipids as potential modulators of intraocular pressure","volume":"24","author":"Edwards","year":"2020","journal-title":"J. Cell Mol. Med."},{"key":"10.1016\/j.exer.2023.109562_bib10","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.biochi.2014.01.020","article-title":"Phospholipid profiles of control and glaucomatous human aqueous humor","volume":"101","author":"Edwards","year":"2014","journal-title":"Biochimie"},{"key":"10.1016\/j.exer.2023.109562_bib11","first-page":"417","article-title":"In vitro and in vivo characterization of disulfide bond use in myocilin complex formation","volume":"10","author":"Fautsch","year":"2004","journal-title":"Mol. Vis."},{"key":"10.1016\/j.exer.2023.109562_bib12","doi-asserted-by":"crossref","first-page":"52","DOI":"10.2174\/1874364101004010052","article-title":"Aqueous humor dynamics: a review","volume":"4","author":"Goel","year":"2010","journal-title":"Open Ophthalmol. J."},{"key":"10.1016\/j.exer.2023.109562_bib13","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0034309","article-title":"Cochlin, intraocular pressure regulation and mechanosensing","volume":"7","author":"Goel","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.exer.2023.109562_bib14","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0170628","article-title":"A comparative study of serum exosome isolation using differential ultracentrifugation and three commercial reagents","volume":"12","author":"Helwa","year":"2017","journal-title":"PLoS One"},{"key":"10.1016\/j.exer.2023.109562_bib15","doi-asserted-by":"crossref","first-page":"38","DOI":"10.3389\/fonc.2012.00038","article-title":"The genomic and proteomic content of cancer cell-derived exosomes","volume":"2","author":"Henderson","year":"2012","journal-title":"Front. Oncol."},{"key":"10.1016\/j.exer.2023.109562_bib16","doi-asserted-by":"crossref","first-page":"237","DOI":"10.12659\/MSMBR.889061","article-title":"Collagen: a potential factor involved in the pathogenesis of glaucoma","volume":"19","author":"Huang","year":"2013","journal-title":"Med. Sci. Monit Basic Res."},{"key":"10.1016\/j.exer.2023.109562_bib17","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1159\/000265263","article-title":"Lipid composition of human aqueous humor","volume":"15","author":"Jahn","year":"1983","journal-title":"Ophthalmic Res."},{"key":"10.1016\/j.exer.2023.109562_bib18","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1111\/ceo.14027","article-title":"Pathogenesis of glaucoma: extracellular matrix dysfunction in the trabecular meshwork-A review","volume":"50","author":"Keller","year":"2022","journal-title":"Clin. Exp. Ophthalmol."},{"key":"10.1016\/j.exer.2023.109562_bib19","doi-asserted-by":"crossref","first-page":"6483","DOI":"10.1167\/iovs.10-5410","article-title":"Intralysosomal iron induces lysosomal membrane permeabilization and cathepsin D-mediated cell death in trabecular meshwork cells exposed to oxidative stress","volume":"51","author":"Lin","year":"2010","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2023.109562_bib20","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1007\/s00216-022-04174-5","article-title":"A review on comparative studies addressing exosome isolation methods from body fluids","volume":"415","author":"Martins","year":"2023","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.exer.2023.109562_bib21","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.ophtha.2021.09.013","article-title":"High-density lipoprotein 3 cholesterol and primary open-angle glaucoma: metabolomics and mendelian randomization analyses","volume":"129","author":"Nusinovici","year":"2022","journal-title":"Ophthalmology"},{"key":"10.1016\/j.exer.2023.109562_bib22","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.1167\/iovs.10-5472","article-title":"Proteomic analyses of corneal tissue subjected to alkali exposure","volume":"52","author":"Parikh","year":"2011","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2023.109562_bib23","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.exer.2006.09.020","article-title":"Myocilin-associated exosomes in human ocular samples","volume":"84","author":"Perkumas","year":"2007","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2023.109562_bib24","doi-asserted-by":"crossref","first-page":"3860","DOI":"10.1167\/iovs.14-16220","article-title":"Increased endoplasmic reticulum stress in human glaucomatous trabecular meshwork cells and tissues","volume":"56","author":"Peters","year":"2015","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2023.109562_bib25","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1038\/nrn2233","article-title":"A neuroscientist's guide to lipidomics","volume":"8","author":"Piomelli","year":"2007","journal-title":"Nat. Rev. Neurosci."},{"key":"10.1016\/j.exer.2023.109562_bib26","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1136\/bjo.2005.081224","article-title":"The number of people with glaucoma worldwide in 2010 and 2020","volume":"90","author":"Quigley","year":"2006","journal-title":"Br. J. Ophthalmol."},{"key":"10.1016\/j.exer.2023.109562_bib27","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1007\/978-1-61779-289-2_13","article-title":"Isolation of exosomes for subsequent mRNA, MicroRNA, and protein profiling","volume":"784","author":"Rani","year":"2011","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.exer.2023.109562_bib28","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1091\/mbc.e05-02-0100","article-title":"Cholesterol is required for efficient endoplasmic reticulum-to-Golgi transport of secretory membrane proteins","volume":"17","author":"Ridsdale","year":"2006","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.exer.2023.109562_bib29","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.molcel.2012.04.015","article-title":"STT3B-dependent posttranslational N-glycosylation as a surveillance system for secretory protein","volume":"47","author":"Sato","year":"2012","journal-title":"Mol. Cell"},{"key":"10.1016\/j.exer.2023.109562_bib30","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/0014-5793(78)80602-4","article-title":"Cathepsin D: cleavage of soluble collagen and crosslinked peptides","volume":"88","author":"Scott","year":"1978","journal-title":"FEBS Lett."},{"key":"10.1016\/j.exer.2023.109562_bib31","doi-asserted-by":"crossref","first-page":"2610","DOI":"10.1016\/j.bbadis.2018.04.016","article-title":"Extracellular vesicle-mediated long-range communication in stressed retinal pigment epithelial cell monolayers","volume":"1864","author":"Shah","year":"2018","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.exer.2023.109562_bib32","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1080\/1354750X.2022.2067233","article-title":"Role of exosomes and its emerging therapeutic applications in the pathophysiology of non-infectious diseases","volume":"27","author":"Shivji","year":"2022","journal-title":"Biomarkers"},{"key":"10.1016\/j.exer.2023.109562_bib33","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1002\/prca.201300031","article-title":"Aberrant glycosylation in the human trabecular meshwork","volume":"8","author":"Sienkiewicz","year":"2014","journal-title":"Proteonomics Clin. Appl."},{"key":"10.1016\/j.exer.2023.109562_bib34","doi-asserted-by":"crossref","first-page":"4258","DOI":"10.1038\/srep04258","article-title":"Inhibition of post-translational N-glycosylation by HRD1 that controls the fate of ABCG5\/8 transporter","volume":"4","author":"Suzuki","year":"2014","journal-title":"Sci. Rep."},{"key":"10.1016\/j.exer.2023.109562_bib35","doi-asserted-by":"crossref","first-page":"3339","DOI":"10.1111\/jcmm.16408","article-title":"Trabecular meshwork's collagen network formation is inhibited by non-pigmented ciliary epithelium-derived extracellular vesicles","volume":"25","author":"Tabak","year":"2021","journal-title":"J. Cell Mol. Med."},{"key":"10.1016\/j.exer.2023.109562_bib36","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1016\/j.exer.2008.11.025","article-title":"Structural changes of the trabecular meshwork in different kinds of glaucoma","volume":"88","author":"Tektas","year":"2009","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2023.109562_bib37","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.biochi.2015.03.019","article-title":"Aqueous humor phospholipids of DBA\/2J and DBA\/2J-Gpnmb(+)\/SjJ mice","volume":"113","author":"Wang","year":"2015","journal-title":"Biochimie"},{"key":"10.1016\/j.exer.2023.109562_bib38","doi-asserted-by":"crossref","first-page":"1901","DOI":"10.1001\/jama.2014.3192","article-title":"The pathophysiology and treatment of glaucoma: a review","volume":"311","author":"Weinreb","year":"2014","journal-title":"JAMA"}],"container-title":["Experimental Eye Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483523001835?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483523001835?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T05:24:19Z","timestamp":1758000259000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014483523001835"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9]]},"references-count":38,"alternative-id":["S0014483523001835"],"URL":"https:\/\/doi.org\/10.1016\/j.exer.2023.109562","relation":{},"ISSN":["0014-4835"],"issn-type":[{"value":"0014-4835","type":"print"}],"subject":[],"published":{"date-parts":[[2023,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Glaucomatous aqueous humor vesicles are smaller and differ in composition compared to controls","name":"articletitle","label":"Article Title"},{"value":"Experimental Eye Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exer.2023.109562","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"109562"}}