{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T08:02:25Z","timestamp":1792828945349,"version":"4.5.0"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170148"],"award-info":[{"award-number":["32170148"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32470100"],"award-info":[{"award-number":["32470100"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Eye Research"],"published-print":{"date-parts":[[2025,2]]},"DOI":"10.1016\/j.exer.2025.110245","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T03:21:44Z","timestamp":1737429704000},"page":"110245","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Screening of a retinal-targeting Adeno-Associated Virus (AAV) via DNA shuffling"],"prefix":"10.1016","volume":"251","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-0047-9298","authenticated-orcid":false,"given":"Yixin","family":"Yu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangwei","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingzhu","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zicheng","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunsheng","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"10","key":"10.1016\/j.exer.2025.110245_bib1","doi-asserted-by":"crossref","DOI":"10.3390\/ijms24108503","article-title":"Boosting the Separation of adeno-associated virus capsid proteins by Liquid chromatography and capillary electrophoresis approaches","volume":"24","author":"Aebischer","year":"2023","journal-title":"Int. J. Mol. Sci."},{"issue":"19\u201320","key":"10.1016\/j.exer.2025.110245_bib2","doi-asserted-by":"crossref","first-page":"6505","DOI":"10.1007\/s00018-021-03917-4","article-title":"A look into retinal organoids: methods, analytical techniques, and applications","volume":"78","author":"Afanasyeva","year":"2021","journal-title":"Cell. Mol. Life Sci."},{"issue":"14","key":"10.1016\/j.exer.2025.110245_bib3","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1007\/s40265-022-01788-y","article-title":"Valoctocogene roxaparvovec: first approval","volume":"82","author":"Blair","year":"2022","journal-title":"Drugs"},{"issue":"24","key":"10.1016\/j.exer.2025.110245_bib4","doi-asserted-by":"crossref","first-page":"9109","DOI":"10.1073\/pnas.0403149101","article-title":"Knock-in human rhodopsin-GFP fusions as mouse models for human disease and targets for gene therapy","volume":"101","author":"Chan","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"4","key":"10.1016\/j.exer.2025.110245_bib5","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1038\/86744","article-title":"DNA shuffling method for generating highly recombined genes and evolved enzymes","volume":"19","author":"Coco","year":"2001","journal-title":"Nat. Biotechnol."},{"issue":"8","key":"10.1016\/j.exer.2025.110245_bib6","doi-asserted-by":"crossref","first-page":"2433","DOI":"10.1016\/j.ymthe.2024.07.001","article-title":"In vivo genome editing for inherited retinal disease: opportunities and challenges","volume":"32","author":"Collin","year":"2024","journal-title":"Mol. Ther."},{"key":"10.1016\/j.exer.2025.110245_bib8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.omtm.2018.05.001","article-title":"Helper-free production of laboratory grade AAV and purification by iodixanol density gradient centrifugation","volume":"10","author":"Crosson","year":"2018","journal-title":"Mol Ther Methods Clin Dev"},{"issue":"9\u201310","key":"10.1016\/j.exer.2025.110245_bib9","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1038\/s41434-024-00477-7","article-title":"The AAV2.7m8 capsid packages a higher degree of heterogeneous vector genomes than AAV2","volume":"31","author":"Cui","year":"2024","journal-title":"Gene Ther."},{"issue":"189","key":"10.1016\/j.exer.2025.110245_bib10","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.3005708","article-title":"In vivo-directed evolution of a new adeno-associated virus for therapeutic outer retinal gene delivery from the vitreous","volume":"5","author":"Dalkara","year":"2013","journal-title":"Sci. Transl. Med."},{"issue":"11","key":"10.1016\/j.exer.2025.110245_bib11","doi-asserted-by":"crossref","DOI":"10.3390\/ijms25115734","article-title":"The ocular glymphatic system-current understanding and future perspectives","volume":"25","author":"Delle","year":"2024","journal-title":"Int. J. Mol. Sci."},{"issue":"7341","key":"10.1016\/j.exer.2025.110245_bib12","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1038\/nature09941","article-title":"Self-organizing optic-cup morphogenesis in three-dimensional culture","volume":"472","author":"Eiraku","year":"2011","journal-title":"Nature"},{"issue":"13\u201314","key":"10.1016\/j.exer.2025.110245_bib13","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1089\/hum.2020.070","article-title":"Engineered capsids for efficient gene delivery to the retina and cornea","volume":"31","author":"Frederick","year":"2020","journal-title":"Hum. Gene Ther."},{"issue":"3","key":"10.1016\/j.exer.2025.110245_bib14","doi-asserted-by":"crossref","first-page":"285","DOI":"10.2174\/1566523054065057","article-title":"New recombinant serotypes of AAV vectors","volume":"5","author":"Gao","year":"2005","journal-title":"Curr. Gene Ther."},{"issue":"Pt 5","key":"10.1016\/j.exer.2025.110245_bib15","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1099\/0022-1317-83-5-973","article-title":"The VP1 capsid protein of adeno-associated virus type 2 is carrying a phospholipase A2 domain required for virus infectivity","volume":"83","author":"Girod","year":"2002","journal-title":"J. Gen. Virol."},{"issue":"15","key":"10.1016\/j.exer.2025.110245_bib16","doi-asserted-by":"crossref","first-page":"7833","DOI":"10.1128\/JVI.00580-07","article-title":"Surface-exposed adeno-associated virus Vp1-NLS capsid fusion protein rescues infectivity of noninfectious wild-type Vp2\/Vp3 and Vp3-only capsids but not that of fivefold pore mutant virions","volume":"81","author":"Grieger","year":"2007","journal-title":"J. Virol."},{"issue":"4","key":"10.1016\/j.exer.2025.110245_bib17","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1007\/s40265-023-01845-0","article-title":"Etranacogene dezaparvovec: first approval","volume":"83","author":"Heo","year":"2023","journal-title":"Drugs"},{"issue":"11","key":"10.1016\/j.exer.2025.110245_bib18","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1007\/s40265-019-01162-5","article-title":"Onasemnogene abeparvovec: first global approval","volume":"79","author":"Hoy","year":"2019","journal-title":"Drugs"},{"issue":"14","key":"10.1016\/j.exer.2025.110245_bib19","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1007\/s40265-023-01929-x","article-title":"Delandistrogene moxeparvovec: first approval","volume":"83","author":"Hoy","year":"2023","journal-title":"Drugs"},{"key":"10.1016\/j.exer.2025.110245_bib20","doi-asserted-by":"crossref","DOI":"10.1002\/0471140864.ps2809s79","article-title":"Analysis of protein stability and ligand interactions by thermal shift assay","volume":"79","author":"Huynh","year":"2015","journal-title":"Curr Protoc Protein Sci"},{"issue":"5","key":"10.1016\/j.exer.2025.110245_bib21","doi-asserted-by":"crossref","first-page":"569","DOI":"10.3109\/02713683.2013.779720","article-title":"Direct comparison of administration routes for AAV8-mediated ocular gene therapy","volume":"38","author":"Igarashi","year":"2013","journal-title":"Curr. Eye Res."},{"issue":"7","key":"10.1016\/j.exer.2025.110245_bib22","doi-asserted-by":"crossref","first-page":"596","DOI":"10.4103\/ijo.IJO_992_16","article-title":"Real-world evidence of safety profile of intravitreal bevacizumab (Avastin) in an Indian scenario","volume":"65","author":"Jain","year":"2017","journal-title":"Indian J. Ophthalmol."},{"key":"10.1016\/j.exer.2025.110245_bib23","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.omtm.2022.07.011","article-title":"Structural basis of receptor usage by the engineered capsid AAV-PHP.eB","volume":"26","author":"Jang","year":"2022","journal-title":"Mol Ther Methods Clin Dev"},{"issue":"3","key":"10.1016\/j.exer.2025.110245_bib24","doi-asserted-by":"crossref","first-page":"235","DOI":"10.2174\/1566523217666171003170348","article-title":"Comparative study of adeno-associated virus, adenovirus, bacu lovirus and lentivirus vectors for gene therapy of the eyes","volume":"17","author":"Kalesnykas","year":"2017","journal-title":"Curr. Gene Ther."},{"issue":"13","key":"10.1016\/j.exer.2025.110245_bib25","first-page":"1427","article-title":"Eladocagene exuparvovec: first approval","volume":"82","author":"Keam","year":"2022","journal-title":"Drugs"},{"key":"10.1016\/j.exer.2025.110245_bib26","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.omtm.2018.11.008","article-title":"Adeno-associated viral vector 2 and 9 transduction is enhanced in streptozotocin-induced diabetic mouse retina","volume":"13","author":"Lee","year":"2019","journal-title":"Mol Ther Methods Clin Dev"},{"issue":"2","key":"10.1016\/j.exer.2025.110245_bib27","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1089\/hgtb.2013.131","article-title":"Absolute determination of single-stranded and self-complementary adeno-associated viral vector genome titers by droplet digital PCR","volume":"25","author":"Lock","year":"2014","journal-title":"Hum. Gene Ther. Methods"},{"issue":"5","key":"10.1016\/j.exer.2025.110245_bib28","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1038\/nrd3572-c1","article-title":"Glybera and the future of gene therapy in the European Union","volume":"11","author":"Miller","year":"2012","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.exer.2025.110245_bib29","series-title":"CADTH Issues In Emerging Health Technologies, 1-11 Ottawa (ON): Canadian Agency for Drugs and Technologies in Health","article-title":"CADTH horizon scans voretigene neparvovec: an emerging gene therapy for the treatment of inherited blindness","author":"Patel","year":"2016"},{"issue":"4","key":"10.1016\/j.exer.2025.110245_bib30","doi-asserted-by":"crossref","DOI":"10.15252\/emmm.202013392","article-title":"Novel AAV capsids for intravitreal gene therapy of photoreceptor disorders","volume":"13","author":"Pavlou","year":"2021","journal-title":"EMBO Mol. Med."},{"issue":"2","key":"10.1016\/j.exer.2025.110245_bib31","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1038\/mt.2010.234","article-title":"Novel properties of tyrosine-mutant AAV2 vectors in the mouse retina","volume":"19","author":"Petrs-Silva","year":"2011","journal-title":"Mol. Ther."},{"issue":"5","key":"10.1016\/j.exer.2025.110245_bib32","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a041312","article-title":"Trial by \"firsts\": clinical trial design and regulatory considerations in the development and approval of the first AAV gene therapy product in the United States","volume":"13","author":"Reape","year":"2023","journal-title":"Cold Spring Harb Perspect Med"},{"key":"10.1016\/j.exer.2025.110245_bib33","first-page":"299","article-title":"Stem cell therapy for retinal degeneration: the evidence to date","volume":"15","author":"Sharma","year":"2021","journal-title":"Biologics"},{"key":"10.1016\/j.exer.2025.110245_bib34","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/978-1-4614-3209-8_19","article-title":"What is the nature of the RGC-5 cell line?","volume":"801","author":"Sippl","year":"2014","journal-title":"Adv. Exp. Med. Biol."},{"issue":"11","key":"10.1016\/j.exer.2025.110245_bib35","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1038\/nri1224","article-title":"Ocular immune privilege: therapeutic opportunities from an experiment of nature","volume":"3","author":"Streilein","year":"2003","journal-title":"Nat. Rev. Immunol."},{"issue":"3","key":"10.1016\/j.exer.2025.110245_bib36","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1016\/j.ymthe.2019.12.010","article-title":"AAV vector immunogenicity in humans: a long journey to successful gene transfer","volume":"28","author":"Verdera","year":"2020","journal-title":"Mol. Ther."},{"issue":"14","key":"10.1016\/j.exer.2025.110245_bib37","doi-asserted-by":"crossref","first-page":"2406","DOI":"10.1093\/hmg\/ddac043","article-title":"Choroidal endothelial and macrophage gene expression in atrophic and neovascular macular degeneration","volume":"31","author":"Voigt","year":"2022","journal-title":"Hum. Mol. Genet."},{"issue":"5","key":"10.1016\/j.exer.2025.110245_bib38","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1038\/s41573-019-0012-9","article-title":"Adeno-associated virus vector as a platform for gene therapy delivery","volume":"18","author":"Wang","year":"2019","journal-title":"Nat. Rev. Drug Discov."},{"issue":"12","key":"10.1016\/j.exer.2025.110245_bib39","doi-asserted-by":"crossref","first-page":"6595","DOI":"10.1128\/JVI.78.12.6595-6609.2004","article-title":"Adeno-associated virus type 2 VP2 capsid protein is nonessential and can tolerate large peptide insertions at its N terminus","volume":"78","author":"Warrington","year":"2004","journal-title":"J. Virol."},{"issue":"2","key":"10.1016\/j.exer.2025.110245_bib40","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1111\/joa.13768","article-title":"Retinal organoids provide unique insights into molecular signatures of inherited retinal disease throughout retinogenesis","volume":"243","author":"Watson","year":"2023","journal-title":"J. Anat."},{"issue":"21\u201322","key":"10.1016\/j.exer.2025.110245_bib41","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1089\/hum.2022.109","article-title":"Identification of broadly applicable adeno-associated virus vectors by systematic comparison of commonly used capsid variants in vitro","volume":"33","author":"Weinmann","year":"2022","journal-title":"Hum. Gene Ther."},{"key":"10.1016\/j.exer.2025.110245_bib42","first-page":"1","article-title":"Adeno-associated virus biology","volume":"807","author":"Weitzman","year":"2011","journal-title":"Methods Mol. Biol."},{"issue":"10","key":"10.1016\/j.exer.2025.110245_bib43","doi-asserted-by":"crossref","first-page":"3946","DOI":"10.1073\/pnas.0813207106","article-title":"A myocardium tropic adeno-associated virus (AAV) evolved by DNA shuffling and in vivo selection","volume":"106","author":"Yang","year":"2009","journal-title":"Proc Natl Acad Sci U S A"}],"container-title":["Experimental Eye Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483525000168?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483525000168?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T04:20:25Z","timestamp":1777350025000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014483525000168"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2]]},"references-count":42,"alternative-id":["S0014483525000168"],"URL":"https:\/\/doi.org\/10.1016\/j.exer.2025.110245","relation":{},"ISSN":["0014-4835"],"issn-type":[{"value":"0014-4835","type":"print"}],"subject":[],"published":{"date-parts":[[2025,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Screening of a retinal-targeting Adeno-Associated Virus (AAV) via DNA shuffling","name":"articletitle","label":"Article Title"},{"value":"Experimental Eye Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exer.2025.110245","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"110245"}}