{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T21:10:27Z","timestamp":1784581827540,"version":"3.55.0"},"reference-count":64,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Eye Research"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1016\/j.exer.2025.110712","type":"journal-article","created":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T00:08:55Z","timestamp":1761782935000},"page":"110712","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Optimizing RT-qPCR for sex-specific analysis of iron-induced retinal gene expression"],"prefix":"10.1016","volume":"262","author":[{"given":"Joan-Feliu","family":"Calafat","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miquel","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gemma","family":"Manich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santiago","family":"Rojas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9454-0734","authenticated-orcid":false,"given":"Judit","family":"Pampalona","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"8","key":"10.1016\/j.exer.2025.110712_bib1","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1111\/gtc.12254","article-title":"Stage\u2010specific reference genes significant for quantitative PCR during mouse retinal development","volume":"20","author":"Adachi","year":"2015","journal-title":"Genes Cells"},{"key":"10.1016\/j.exer.2025.110712_bib2","author":"Al-Shabrawey"},{"issue":"15","key":"10.1016\/j.exer.2025.110712_bib3","doi-asserted-by":"crossref","first-page":"5245","DOI":"10.1158\/0008-5472.CAN-04-0496","article-title":"Normalization of real-time quantitative reverse Transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and Colon cancer data sets","volume":"64","author":"Andersen","year":"2004","journal-title":"Cancer Res."},{"issue":"9","key":"10.1016\/j.exer.2025.110712_bib4","doi-asserted-by":"crossref","first-page":"1814","DOI":"10.1016\/j.ajpath.2019.05.022","article-title":"Liver-specific, but not retina-specific, hepcidin knockout causes retinal iron accumulation and degeneration","volume":"189","author":"Baumann","year":"2019","journal-title":"Am. J. Pathol."},{"issue":"3","key":"10.1016\/j.exer.2025.110712_bib5","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1167\/iovs.64.3.22","article-title":"Ferritin but not iron increases in retina upon systemic iron overload in diabetic and iron-dextran injected mice","volume":"64","author":"Bonet","year":"2023","journal-title":"Investigative Opthalmol. Visual Sci."},{"issue":"4","key":"10.1016\/j.exer.2025.110712_bib6","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments","volume":"55","author":"Bustin","year":"2009","journal-title":"Clin. Chem."},{"issue":"1","key":"10.1016\/j.exer.2025.110712_bib7","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1186\/1471-2199-9-49","article-title":"Exercise induced stress in horses: selection of the most stable reference genes for quantitative RT-PCR normalization","volume":"9","author":"Cappelli","year":"2008","journal-title":"BMC Mol. Biol."},{"issue":"8","key":"10.1016\/j.exer.2025.110712_bib8","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0136056","article-title":"Differences in retinal structure and function between aging Male and female sprague-dawley rats are strongly influenced by the estrus cycle","volume":"10","author":"Chaychi","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.exer.2025.110712_bib9","first-page":"1","article-title":"Selection of suitable reference genes for qPCR gene expression analysis of HepG2 and L02 in four different liver cell injured models","author":"Chen","year":"2020","journal-title":"BioMed Res. Int."},{"issue":"2","key":"10.1016\/j.exer.2025.110712_bib10","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.bcmd.2003.11.003","article-title":"Strain and gender modulate hepatic hepcidin 1 and 2 mRNA expression in mice","volume":"32","author":"Courselaud","year":"2004","journal-title":"Blood Cell Mol. Dis."},{"issue":"1","key":"10.1016\/j.exer.2025.110712_bib11","doi-asserted-by":"crossref","first-page":"9518","DOI":"10.1038\/s41598-024-58863-5","article-title":"Reference genes to study the sex-biased expression of genes regulating Drosophila metabolism","volume":"14","author":"De Groef","year":"2024","journal-title":"Sci. Rep."},{"issue":"3","key":"10.1016\/j.exer.2025.110712_bib12","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0122515","article-title":"Reference gene validation for RT-qPCR, a note on different available software packages","volume":"10","author":"De Spiegelaere","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.exer.2025.110712_bib13","doi-asserted-by":"crossref","DOI":"10.1146\/annurev-biochem-050410-100030","article-title":"Structure and regulation of soluble guanylate cyclase","volume":"81","author":"Derbyshire","year":"2012","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.exer.2025.110712_bib14","doi-asserted-by":"crossref","DOI":"10.1186\/1741-7007-6-49","article-title":"A comprehensive functional analysis of tissue specificity of human gene expression","volume":"6","author":"Dezs\u00f6","year":"2008","journal-title":"BMC Biol."},{"issue":"2","key":"10.1016\/j.exer.2025.110712_bib15","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/0006-8993(94)91316-1","article-title":"The influences of age, retinal topography, and gender on retinal degeneration in the Fischer 344 rat","volume":"647","author":"DiLoreto","year":"1994","journal-title":"Brain Res."},{"issue":"7","key":"10.1016\/j.exer.2025.110712_bib16","article-title":"Reporting animal research: explanation and elaboration for the arrive guidelines 2.0","volume":"18","author":"du Sert","year":"2020","journal-title":"PLoS Biol."},{"issue":"1","key":"10.1016\/j.exer.2025.110712_bib17","doi-asserted-by":"crossref","first-page":"29","DOI":"10.3390\/molecules28010029","article-title":"Mitochondrial Iron metabolism: the crucial actors in diseases","volume":"28","author":"Duan","year":"2022","journal-title":"Molecules"},{"issue":"4","key":"10.1016\/j.exer.2025.110712_bib18","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/j.exer.2008.01.010","article-title":"Subtype specific estrogen receptor action protects against changes in MMP-2 activation in mouse retinal pigmented epithelial cells","volume":"86","author":"Elliot","year":"2008","journal-title":"Exp. Eye Res."},{"issue":"7912","key":"10.1016\/j.exer.2025.110712_bib19","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1038\/s41586-022-04686-1","article-title":"Gene regulation by gonadal hormone receptors underlies brain sex differences","volume":"606","author":"Gegenhuber","year":"2022","journal-title":"Nature"},{"issue":"1\u20132","key":"10.1016\/j.exer.2025.110712_bib20","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.brainres.2004.04.040","article-title":"Retinal oxidation, apoptosis and age- and sex-differences in the mnd mutant mouse, a model of neuronal ceroid lipofuscinosis","volume":"1014","author":"Guarneri","year":"2004","journal-title":"Brain Res."},{"issue":"2","key":"10.1016\/j.exer.2025.110712_bib21","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/S0014-5793(01)03189-1","article-title":"Iron\u2010dependent regulation of the divalent metal ion transporter","volume":"509","author":"Gunshin","year":"2001","journal-title":"FEBS (Fed. Eur. Biochem. Soc.) Lett."},{"issue":"6641","key":"10.1016\/j.exer.2025.110712_bib22","doi-asserted-by":"crossref","DOI":"10.1038\/41343","article-title":"Cloning and characterization of a mammalian proton-coupled metal-ion transporter","volume":"388","author":"Gunshin","year":"1997","journal-title":"Nature"},{"issue":"1","key":"10.1016\/j.exer.2025.110712_bib23","article-title":"Age-dependent retinal iron accumulation and degeneration in hepcidin knockout mice","volume":"52","author":"Hadziahmetovic","year":"2011","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"issue":"2","key":"10.1016\/j.exer.2025.110712_bib24","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1167\/iovs.10-6207","article-title":"The oral iron chelator deferiprone protects against iron overload\u2013induced retinal degeneration","volume":"52","author":"Hadziahmetovic","year":"2011","journal-title":"Investigative Opthalmol. Visual Sci."},{"issue":"1","key":"10.1016\/j.exer.2025.110712_bib25","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.ajpath.2011.03.033","article-title":"Bmp6 regulates retinal iron homeostasis and has altered expression in age-related macular degeneration","volume":"179","author":"Hadziahmetovic","year":"2011","journal-title":"Am. J. Pathol."},{"issue":"6","key":"10.1016\/j.exer.2025.110712_bib26","doi-asserted-by":"crossref","first-page":"E582","DOI":"10.1152\/ajpendo.1983.245.6.E582","article-title":"Suppression of norepinephrine-induced thermogenesis in brown adipose tissue by fasting","volume":"245","author":"Hayashi","year":"1983","journal-title":"Am. J. Physiol. Endocrinol. Metabol."},{"issue":"2","key":"10.1016\/j.exer.2025.110712_bib27","doi-asserted-by":"crossref","first-page":"R19","DOI":"10.1186\/gb-2007-8-2-r19","article-title":"qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data","volume":"8","author":"Hellemans","year":"2007","journal-title":"Genome Biol."},{"key":"10.1016\/j.exer.2025.110712_bib28","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2020.593514","article-title":"Changes in retinal structure and ultrastructure in the aged mice correlate with differences in the expression of selected retinal miRNAs","volume":"11","author":"Hermenean","year":"2021","journal-title":"Front. Pharmacol."},{"issue":"1","key":"10.1016\/j.exer.2025.110712_bib29","doi-asserted-by":"crossref","first-page":"3278","DOI":"10.1038\/s41598-021-82800-5","article-title":"Assessment of common housekeeping genes as reference for gene expression studies using RT-qPCR in mouse choroid plexus","volume":"11","author":"Ho","year":"2021","journal-title":"Sci. Rep."},{"issue":"19","key":"10.1016\/j.exer.2025.110712_bib30","doi-asserted-by":"crossref","first-page":"12345","DOI":"10.1073\/pnas.192423399","article-title":"Previously uncharacterized isoforms of divalent metal transporter (DMT)-1: implications for regulation and cellular function","volume":"99","author":"Hubert","year":"2002","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.exer.2025.110712_bib31","first-page":"1","article-title":"Real-time quantitative PCR","author":"Johnson","year":"2013","journal-title":"Pathogen Detect. MIQE"},{"issue":"15","key":"10.1016\/j.exer.2025.110712_bib32","doi-asserted-by":"crossref","first-page":"R642","DOI":"10.1016\/j.cub.2013.05.033","article-title":"The essential nature of iron usage and regulation","volume":"23","author":"Kaplan","year":"2013","journal-title":"Curr. Biol."},{"issue":"6","key":"10.1016\/j.exer.2025.110712_bib33","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1016\/j.jhep.2004.02.029","article-title":"Different expression pattern of hepcidin genes in the liver and pancreas of C57BL\/6N and DBA\/2N mice","volume":"40","author":"Krijt","year":"2004","journal-title":"J. Hepatol."},{"issue":"1","key":"10.1016\/j.exer.2025.110712_bib34","doi-asserted-by":"crossref","DOI":"10.1080\/21623945.2024.2330355","article-title":"A comparative assessment of reference genes in mouse brown adipocyte differentiation and thermogenesis in vitro","volume":"13","author":"Lai","year":"2024","journal-title":"Adipocyte"},{"issue":"2","key":"10.1016\/j.exer.2025.110712_bib35","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1002\/hep.26648","article-title":"Testosterone perturbs systemic iron balance through activation of epidermal growth factor receptor signaling in the liver and repression of hepcidin","volume":"59","author":"Latour","year":"2014","journal-title":"Hepatology"},{"issue":"1","key":"10.1016\/j.exer.2025.110712_bib36","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.jneumeth.2011.05.026","article-title":"Normalization of gene expression using SYBR green qPCR: a case for paraoxonase 1 and 2 in Alzheimer's disease brains","volume":"200","author":"Leduc","year":"2011","journal-title":"J. Neurosci. Methods"},{"key":"10.1016\/j.exer.2025.110712_bib37","doi-asserted-by":"crossref","DOI":"10.1016\/j.exer.2019.107773","article-title":"Sex-related differences in the progressive retinal degeneration of the rd10 mouse","volume":"187","author":"Li","year":"2019","journal-title":"Exp. Eye Res."},{"issue":"4","key":"10.1016\/j.exer.2025.110712_bib38","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"issue":"23","key":"10.1016\/j.exer.2025.110712_bib39","doi-asserted-by":"crossref","DOI":"10.3390\/ijms242316661","article-title":"Animal models in eye research: focus on corneal pathologies","volume":"24","author":"Loiseau","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.exer.2025.110712_bib40","article-title":"Retinal gene expression responses to aging are sexually divergent","volume":"23","author":"Mei","year":"2017","journal-title":"Mol. Vis."},{"issue":"9","key":"10.1016\/j.exer.2025.110712_bib41","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0106974","article-title":"L-ferritin binding to Scara5: a new iron traffic pathway potentially implicated in retinopathy","volume":"9","author":"Mendes-Jorge","year":"2014","journal-title":"PLoS One"},{"issue":"4","key":"10.1016\/j.exer.2025.110712_bib42","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1261\/rna.063941.117","article-title":"Regulation of transferrin receptor-1 mRNA by the interplay between IRE-binding proteins and miR-7\/miR-141 in the 3\u2032-IRE stem\u2013loops","volume":"24","author":"Miyazawa","year":"2018","journal-title":"RNA"},{"issue":"5","key":"10.1016\/j.exer.2025.110712_bib43","doi-asserted-by":"crossref","DOI":"10.1074\/jbc.M508903200","article-title":"RPE65 is an iron(II)-dependent isomerohydrolase in the retinoid visual cycle","volume":"281","author":"Moiseyev","year":"2006","journal-title":"J. Biol. Chem."},{"issue":"4","key":"10.1016\/j.exer.2025.110712_bib44","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0284764","article-title":"Identification of reference genes for the normalization of retinal mRNA expression by RT-qPCR in oxygen induced retinopathy, anemia, and erythropoietin administration","volume":"18","author":"Molomjamts","year":"2023","journal-title":"PLoS One"},{"issue":"6","key":"10.1016\/j.exer.2025.110712_bib45","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1023\/B:BILE.0000019559.84305.47","article-title":"Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: bestkeeper \u2013 excel-based tool using pair-wise correlations","volume":"26","author":"Pfaffl","year":"2004","journal-title":"Biotechnol. Lett."},{"issue":"Issue 3","key":"10.1016\/j.exer.2025.110712_bib46","article-title":"From rust to quantum biology: the role of iron in retina physiopathology","volume":"9","author":"Picard","year":"2020","journal-title":"Cells"},{"issue":"8","key":"10.1016\/j.exer.2025.110712_bib47","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0043028","article-title":"Avoiding pitfalls of internal controls: validation of reference genes for analysis by qRT-PCR and Western blot throughout rat retinal development","volume":"7","author":"Rocha-Martins","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.exer.2025.110712_bib48","doi-asserted-by":"crossref","DOI":"10.1038\/srep37116","article-title":"Assessment of brain reference genes for RT-qPCR studies in neurodegenerative diseases","volume":"6","author":"Rydbirk","year":"2016","journal-title":"Sci. Rep."},{"issue":"1","key":"10.1016\/j.exer.2025.110712_bib49","doi-asserted-by":"crossref","DOI":"10.1038\/srep37116","article-title":"Assessment of brain reference genes for RT-qPCR studies in neurodegenerative diseases","volume":"6","author":"Rydbirk","year":"2016","journal-title":"Sci. Rep."},{"issue":"3","key":"10.1016\/j.exer.2025.110712_bib50","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1167\/iovs.64.3.18","article-title":"Tissue-specific sex difference in mouse eye and brain metabolome under fed and fasted states","volume":"64","author":"Saravanan","year":"2023","journal-title":"Investigative Opthalmol. Visual Sci."},{"issue":"13","key":"10.1016\/j.exer.2025.110712_bib51","article-title":"Iron accumulates in retinal vascular endothelial cells but has minimal retinal penetration after IP iron dextran injection in mice","volume":"60","author":"Shu","year":"2019","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"issue":"1","key":"10.1016\/j.exer.2025.110712_bib52","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/1471-2199-7-33","article-title":"Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR","volume":"7","author":"Silver","year":"2006","journal-title":"BMC Mol. Biol."},{"issue":"JUN","key":"10.1016\/j.exer.2025.110712_bib53","article-title":"Retinal iron homeostasis in health and disease","volume":"5","author":"Song","year":"2013","journal-title":"Front. Aging Neurosci."},{"key":"10.1016\/j.exer.2025.110712_bib54","first-page":"317","article-title":"Gender differences in retinal diseases: a review","volume":"vol. 52","author":"Tillmann","year":"2024"},{"issue":"5","key":"10.1016\/j.exer.2025.110712_bib55","doi-asserted-by":"crossref","DOI":"10.1097\/HS9.0000000000000459","article-title":"Control of systemic iron homeostasis by the 3\u2019 iron\u2010responsive element of divalent metal transporter 1 in mice","volume":"4","author":"Tybl","year":"2020","journal-title":"HemaSphere"},{"issue":"15","key":"10.1016\/j.exer.2025.110712_bib56","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gks596","article-title":"Primer3\u2014new capabilities and interfaces","volume":"40","author":"Untergasser","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.exer.2025.110712_bib57","first-page":"1508","article-title":"Stability of housekeeping gene expression in the rat retina during exposure to cyclic hyperoxia","volume":"13","author":"van Wijngaarden","year":"2007","journal-title":"Mol. Vis."},{"issue":"7","key":"10.1016\/j.exer.2025.110712_bib58","doi-asserted-by":"crossref","DOI":"10.1186\/gb-2002-3-7-research0034","article-title":"Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes","volume":"3","author":"Vandesompele","year":"2002","journal-title":"Genome Biol."},{"issue":"2","key":"10.1016\/j.exer.2025.110712_bib59","doi-asserted-by":"crossref","first-page":"263","DOI":"10.3390\/ijms18020263","article-title":"Mrpl10 and tbp are suitable reference genes for peripheral nerve crush injury","volume":"18","author":"Wang","year":"2017","journal-title":"Int. J. Mol. Sci."},{"issue":"2","key":"10.1016\/j.exer.2025.110712_bib60","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/s10142-023-01055-7","article-title":"RefFinder: a web-based tool for comprehensively analyzing and identifying reference genes","volume":"23","author":"Xie","year":"2023","journal-title":"Funct. Integr. Genom."},{"issue":"1","key":"10.1016\/j.exer.2025.110712_bib61","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s11103-012-9885-2","article-title":"miRDeepFinder: a miRNA analysis tool for deep sequencing of plant small RNAs","volume":"80","author":"Xie","year":"2012","journal-title":"Plant Mol. Biol."},{"issue":"1","key":"10.1016\/j.exer.2025.110712_bib62","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/1471-2121-11-39","article-title":"Heme and non-heme iron transporters in non-polarized and polarized cells","volume":"11","author":"Yanatori","year":"2010","journal-title":"BMC Cell Biol."},{"issue":"11","key":"10.1016\/j.exer.2025.110712_bib64","doi-asserted-by":"crossref","first-page":"2862","DOI":"10.1016\/j.ajpath.2014.07.008","article-title":"A high serum iron level causes mouse retinal iron accumulation despite an intact blood-retinal barrier","volume":"184","author":"Zhao","year":"2014","journal-title":"Am. J. Pathol."},{"issue":"4","key":"10.1016\/j.exer.2025.110712_bib65","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1093\/jn\/nxy012","article-title":"Multi-copper ferroxidase\u2013deficient mice have increased brain iron concentrations and learning and memory deficits","volume":"148","author":"Zheng","year":"2018","journal-title":"J. Nutr."}],"container-title":["Experimental Eye Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483525004853?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483525004853?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T08:35:47Z","timestamp":1781253347000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014483525004853"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":64,"alternative-id":["S0014483525004853"],"URL":"https:\/\/doi.org\/10.1016\/j.exer.2025.110712","relation":{},"ISSN":["0014-4835"],"issn-type":[{"value":"0014-4835","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Optimizing RT-qPCR for sex-specific analysis of iron-induced retinal gene expression","name":"articletitle","label":"Article Title"},{"value":"Experimental Eye Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exer.2025.110712","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"110712"}}