{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T06:27:07Z","timestamp":1793600827548,"version":"4.5.0"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Korea Ministry of Science and ICT","doi-asserted-by":"publisher","award":["NRF-2021R1C1C2009518"],"award-info":[{"award-number":["NRF-2021R1C1C2009518"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Eye Research"],"published-print":{"date-parts":[[2026,6]]},"DOI":"10.1016\/j.exer.2026.110980","type":"journal-article","created":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T00:08:41Z","timestamp":1774051721000},"page":"110980","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Experimental modeling of Acanthamoeba keratitis in mice: Isolation and cultivation of Acanthamoeba castellanii"],"prefix":"10.1016","volume":"267","author":[{"given":"Hae-Jin","family":"Sohn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suk-Yul","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong-Hyun","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.exer.2026.110980_bib1","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.clae.2015.11.004","article-title":"Targeting cyst wall is an effective strategy in improving the efficacy of marketed contact lens disinfecting solutions against Acanthamoeba castellanii cysts","volume":"39","author":"Abjani","year":"2016","journal-title":"Contact Lens Anterior Eye"},{"key":"10.1016\/j.exer.2026.110980_bib2","article-title":"Perspectives on the incidence of Acanthamoeba keratitis: a systematic review and meta-analysis","author":"Aiello","year":"2024","journal-title":"Ophthalmology"},{"issue":"2","key":"10.1016\/j.exer.2026.110980_bib3","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1097\/00003226-199503000-00012","article-title":"Successful immunization against Acanthamoeba keratitis in a pig model","volume":"14","author":"Alizadeh","year":"1995","journal-title":"Cornea"},{"issue":"6","key":"10.1016\/j.exer.2026.110980_bib4","doi-asserted-by":"crossref","DOI":"10.3390\/diagnostics12061344","article-title":"Intelligent diagnosis and classification of Keratitis","volume":"12","author":"Alquran","year":"2022","journal-title":"Diagnostics"},{"key":"10.1016\/j.exer.2026.110980_bib5","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s40135-020-00252-y","article-title":"Photodynamic therapy for infectious keratitis","volume":"8","author":"Altamirano","year":"2020","journal-title":"Current ophthalmol. reports"},{"issue":"3","key":"10.1016\/j.exer.2026.110980_bib6","doi-asserted-by":"crossref","DOI":"10.1111\/pim.12692","article-title":"Experimental keratitis in rats caused by Acanthamoeba griffini: a kinetic histopathological study","volume":"42","author":"\u00c1vila\u2010Blanco","year":"2020","journal-title":"Parasite Immunol."},{"issue":"1-2","key":"10.1016\/j.exer.2026.110980_bib7","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.molbiopara.2016.03.009","article-title":"Characterisation and expression analysis of trophozoite and cyst proteins of Acanthamoeba spp. isolated from Acanthamoeba keratitis (AK) patient","volume":"205","author":"Behera","year":"2016","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.exer.2026.110980_bib8","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1159\/000414336","article-title":"Genetic studies on resistant and susceptibility genes controlling the mouse cornea to infection with Pseudomonas aeruginosa","volume":"39","author":"Berk","year":"1987","journal-title":"Antibiot. Chemother."},{"issue":"2","key":"10.1016\/j.exer.2026.110980_bib9","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1097\/ICO.0b013e31826429bd","article-title":"Prognostic factors associated with the need for surgical treatments in Acanthamoeba keratitis","volume":"32","author":"Bouheraoua","year":"2013","journal-title":"Cornea"},{"issue":"21","key":"10.1016\/j.exer.2026.110980_bib10","doi-asserted-by":"crossref","first-page":"3358","DOI":"10.3390\/diagnostics13213358","article-title":"Updates in diagnostic imaging for infectious keratitis: a review","volume":"13","author":"Cabrera-Aguas","year":"2023","journal-title":"Diagnostics"},{"issue":"4","key":"10.1016\/j.exer.2026.110980_bib11","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.ajo.2009.06.009","article-title":"Acanthamoeba keratitis: diagnosis and treatment update 2009","volume":"148","author":"Dart","year":"2009","journal-title":"Am. J. Ophthalmol."},{"issue":"1","key":"10.1016\/j.exer.2026.110980_bib12","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1111\/opo.12752","article-title":"Acanthamoeba keratitis: a review of biology, pathophysiology and epidemiology","volume":"41","author":"de Lacerda","year":"2021","journal-title":"Ophthalmic Physiol. Opt."},{"issue":"3","key":"10.1016\/j.exer.2026.110980_bib13","doi-asserted-by":"crossref","first-page":"323","DOI":"10.3390\/pathogens10030323","article-title":"Acanthamoeba keratitis, pathology, diagnosis and treatment","volume":"10","author":"Fanselow","year":"2021","journal-title":"Pathogens"},{"key":"10.1016\/j.exer.2026.110980_bib14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12348-018-0161-8","article-title":"Comparative in vitro effectiveness of a novel contact lens multipurpose solution on Acanthamoeba castellanii","volume":"8","author":"Fears","year":"2018","journal-title":"Journal of ophthalmic inflammation and infection"},{"issue":"4","key":"10.1016\/j.exer.2026.110980_bib15","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1111\/j.1574-6976.2006.00023.x","article-title":"Acanthamoeba: biology and increasing importance in human health","volume":"30","author":"Khan","year":"2006","journal-title":"FEMS Microbiol. Rev."},{"issue":"4","key":"10.1016\/j.exer.2026.110980_bib16","doi-asserted-by":"crossref","first-page":"235","DOI":"10.3109\/09273949709085064","article-title":"Systemic immune response to Acanthamoeba keratitis in the Chinese hamster","volume":"5","author":"Klink","year":"1997","journal-title":"Ocul. Immunol. Inflamm."},{"issue":"1","key":"10.1016\/j.exer.2026.110980_bib17","article-title":"Total penetrating keratoplasty: indications, therapeutic approach, and long\u2010term Follow\u2010Up","volume":"2018","author":"Krysik","year":"2018","journal-title":"J. ophthalmol."},{"key":"10.1016\/j.exer.2026.110980_bib18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12886-019-1210-2","article-title":"Acanthamoeba keratitis related to contact lens use in a tertiary hospital in China","volume":"19","author":"Li","year":"2019","journal-title":"BMC Ophthalmol."},{"issue":"1","key":"10.1016\/j.exer.2026.110980_bib19","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.ajo.2007.08.040","article-title":"Comparison of polyhexamethylene biguanide and chlorhexidine as monotherapy agents in the treatment of Acanthamoeba keratitis","volume":"145","author":"Lim","year":"2008","journal-title":"Am. J. Ophthalmol."},{"issue":"4","key":"10.1016\/j.exer.2026.110980_bib20","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1097\/00003226-198804000-00013","article-title":"Acanthamoeba-contaminated hydrogel contact lenses","volume":"7","author":"Lindquist","year":"1988","journal-title":"Cornea"},{"key":"10.1016\/j.exer.2026.110980_bib21","doi-asserted-by":"crossref","DOI":"10.1051\/parasite\/2015010","article-title":"An update on Acanthamoeba keratitis: diagnosis, pathogenesis and treatment","volume":"22","author":"Lorenzo-Morales","year":"2015","journal-title":"Parasite"},{"issue":"2","key":"10.1016\/j.exer.2026.110980_bib22","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1128\/CMR.16.2.273-307.2003","article-title":"Acanthamoeba spp. as agents of disease in humans","volume":"16","author":"Marciano-Cabral","year":"2003","journal-title":"Clin. Microbiol. Rev."},{"issue":"1","key":"10.1016\/j.exer.2026.110980_bib23","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1111\/j.1550-7408.2000.tb00007.x","article-title":"The increasing importance of Acanthamoeba infections","volume":"47","author":"Marciano-Cabral","year":"2000","journal-title":"J. Eukaryot. Microbiol."},{"issue":"5","key":"10.1016\/j.exer.2026.110980_bib24","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1097\/ICO.0000000000000804","article-title":"Update on Acanthamoeba keratitis: diagnosis, treatment, and outcomes","volume":"35","author":"Maycock","year":"2016","journal-title":"Cornea"},{"issue":"4","key":"10.1016\/j.exer.2026.110980_bib25","article-title":"Assessment of in vitro dynamics of pathogenic Acanthamoeba strains originating from contact lens wearers with infectious keratitis","volume":"62","author":"Padzik","year":"2016","journal-title":"Annals of Parasitology"},{"issue":"4","key":"10.1016\/j.exer.2026.110980_bib26","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1136\/bjophthalmol-2019-314485","article-title":"Acanthamoeba keratitis therapy: time to cure and visual outcome analysis for different antiamoebic therapies in 227 cases","volume":"104","author":"Papa","year":"2020","journal-title":"Br. J. Ophthalmol."},{"issue":"12","key":"10.1016\/j.exer.2026.110980_bib27","doi-asserted-by":"crossref","first-page":"2377","DOI":"10.3390\/microorganisms10122377","article-title":"The anti-amoebic activity of a peptidomimetic against Acanthamoeba castellanii","volume":"10","author":"Peguda","year":"2022","journal-title":"Microorganisms"},{"key":"10.1016\/j.exer.2026.110980_bib28","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1007\/s00436-007-0704-7","article-title":"Clinical and histologic evaluations of experimental Acanthamoeba keratitis","volume":"101","author":"Polat","year":"2007","journal-title":"Parasitol. Res."},{"issue":"1","key":"10.1016\/j.exer.2026.110980_bib29","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1186\/s13063-024-08376-3","article-title":"A double-masked, sham-controlled trial of rose bengal photodynamic therapy for the treatment of fungal and acanthamoeba keratitis: Rose Bengal Electromagnetic Activation with Green Light for Infection Reduction (REAGIR) study","volume":"25","author":"Prajna","year":"2024","journal-title":"Trials"},{"issue":"1","key":"10.1016\/j.exer.2026.110980_bib30","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0011878","article-title":"The role of naturally acquired intracellular Pseudomonas aeruginosa in the development of Acanthamoeba keratitis in an animal model","volume":"18","author":"Rayamajhee","year":"2024","journal-title":"PLoS Neglected Trop. Dis."},{"issue":"12","key":"10.1016\/j.exer.2026.110980_bib31","doi-asserted-by":"crossref","first-page":"1484","DOI":"10.1080\/02713683.2020.1772830","article-title":"Development and assessment of a simulator for in vivo confocal microscopy in fungal and Acanthamoeba keratitis","volume":"45","author":"Roth","year":"2020","journal-title":"Curr. Eye Res."},{"issue":"6","key":"10.1016\/j.exer.2026.110980_bib32","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1080\/02713680490522470","article-title":"Local and systemic humoral immune response during acute and chronic Acanthamoeba keratitis in rabbits","volume":"29","author":"Said","year":"2004","journal-title":"Curr. Eye Res."},{"issue":"4","key":"10.1016\/j.exer.2026.110980_bib33","doi-asserted-by":"crossref","DOI":"10.3390\/biom14040389","article-title":"Infectious keratitis: characterization of microbial diversity through Species richness and Shannon diversity index","volume":"14","author":"Schiano-Lomoriello","year":"2024","journal-title":"Biomolecules"},{"issue":"9","key":"10.1016\/j.exer.2026.110980_bib34","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1016\/j.ijpara.2004.06.004","article-title":"Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals","volume":"34","author":"Schuster","year":"2004","journal-title":"Int. J. Parasitol."},{"issue":"12","key":"10.1016\/j.exer.2026.110980_bib35","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.3390\/biology12121489","article-title":"New frontiers in Acanthamoeba Keratitis diagnosis and management","volume":"12","author":"Shareef","year":"2023","journal-title":"Biology"},{"issue":"3","key":"10.1016\/j.exer.2026.110980_bib36","doi-asserted-by":"crossref","first-page":"233","DOI":"10.3347\/kjp.2017.55.3.233","article-title":"Efficient liquid media for encystation of pathogenic free-living Amoebae","volume":"55","author":"Sohn","year":"2017","journal-title":"Kor. J. Parasitol."},{"issue":"8","key":"10.1016\/j.exer.2026.110980_bib37","doi-asserted-by":"crossref","first-page":"2687","DOI":"10.1128\/JCM.37.8.2687-2693.1999","article-title":"Fluorescent oligonucleotide probes for clinical and environmental detection of Acanthamoeba and the T4 18S rRNA gene sequence type","volume":"37","author":"Stothard","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.exer.2026.110980_bib38","series-title":"Acanthamoeba Keratitis: Diagnosis and Treatment","author":"Sun","year":"2018"},{"issue":"10","key":"10.1016\/j.exer.2026.110980_bib39","doi-asserted-by":"crossref","first-page":"2059","DOI":"10.1016\/j.ophtha.2014.04.013","article-title":"In vitro evaluations of topical agents to treat Acanthamoeba keratitis","volume":"121","author":"Sunada","year":"2014","journal-title":"Ophthalmology"},{"issue":"5","key":"10.1016\/j.exer.2026.110980_bib40","doi-asserted-by":"crossref","first-page":"505","DOI":"10.3347\/kjp.2019.57.5.505","article-title":"The first Acanthamoeba keratitis case of non-contact lens wearer with HIV infection in Thailand","volume":"57","author":"Tananuvat","year":"2019","journal-title":"Kor. J. Parasitol."},{"issue":"7","key":"10.1016\/j.exer.2026.110980_bib41","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1097\/ICO.0b013e31816f27bf","article-title":"The relative value of confocal microscopy and superficial corneal scrapings in the diagnosis of Acanthamoeba keratitis","volume":"27","author":"Tu","year":"2008","journal-title":"Cornea"},{"issue":"1","key":"10.1016\/j.exer.2026.110980_bib42","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.ophtha.2010.05.018","article-title":"Role of confocal microscopy in the diagnosis of fungal and acanthamoeba keratitis","volume":"118","author":"Vaddavalli","year":"2011","journal-title":"Ophthalmology"},{"issue":"6","key":"10.1016\/j.exer.2026.110980_bib43","first-page":"1937","article-title":"The role of contact lenses, trauma, and Langerhans cells in a Chinese hamster model of Acanthamoeba keratitis","volume":"34","author":"van Klink","year":"1993","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"issue":"5","key":"10.1016\/j.exer.2026.110980_bib44","doi-asserted-by":"crossref","first-page":"942","DOI":"10.3390\/jcm10050942","article-title":"Challenges in Acanthamoeba keratitis: a review","volume":"10","author":"Varacalli","year":"2021","journal-title":"J. Clin. Med."},{"issue":"2","key":"10.1016\/j.exer.2026.110980_bib45","doi-asserted-by":"crossref","DOI":"10.1016\/j.clae.2023.102119","article-title":"Clinical outcomes and prognostic factors in Acanthamoeba keratitis","volume":"47","author":"Vilares-Morgado","year":"2024","journal-title":"Contact Lens Anterior Eye"},{"issue":"4","key":"10.1016\/j.exer.2026.110980_bib46","doi-asserted-by":"crossref","first-page":"25s","DOI":"10.1111\/j.1550-7408.1990.tb01142.x","article-title":"Epidemiology of free-living ameba infections","volume":"37","author":"Visvesvara","year":"1990","journal-title":"J. Protozool."},{"issue":"1","key":"10.1016\/j.exer.2026.110980_bib47","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1167\/iovs.02-0446","article-title":"Experimental keratomycosis in a mouse model","volume":"44","author":"Wu","year":"2003","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"issue":"4","key":"10.1016\/j.exer.2026.110980_bib48","doi-asserted-by":"crossref","first-page":"221","DOI":"10.3109\/09286586.2012.681336","article-title":"Acanthamoeba keratitis: the persistence of cases following a multistate outbreak","volume":"19","author":"Yoder","year":"2012","journal-title":"Ophthalmic Epidemiol."},{"issue":"6","key":"10.1016\/j.exer.2026.110980_bib49","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/j.jiph.2023.03.020","article-title":"The global epidemiology and clinical diagnosis of Acanthamoeba keratitis","volume":"16","author":"Zhang","year":"2023","journal-title":"J. Infect. Public Health"}],"container-title":["Experimental Eye Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483526001363?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483526001363?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T05:35:00Z","timestamp":1793597700000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014483526001363"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":49,"alternative-id":["S0014483526001363"],"URL":"https:\/\/doi.org\/10.1016\/j.exer.2026.110980","relation":{},"ISSN":["0014-4835"],"issn-type":[{"value":"0014-4835","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Experimental modeling of Acanthamoeba keratitis in mice: Isolation and cultivation of Acanthamoeba castellanii","name":"articletitle","label":"Article Title"},{"value":"Experimental Eye Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exer.2026.110980","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"110980"}}