{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T22:02:22Z","timestamp":1793397742679,"version":"4.5.0"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004320","name":"Shiraz University of Medical Sciences","doi-asserted-by":"publisher","award":["29360"],"award-info":[{"award-number":["29360"]}],"id":[{"id":"10.13039\/501100004320","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004883","name":"Shahrekord University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004883","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Eye Research"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.exer.2026.111123","type":"journal-article","created":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T19:57:36Z","timestamp":1781207856000},"page":"111123","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Development and evaluation of a multi-panel real-time PCR assay for rapid detection of six bacterial species causing endophthalmitis"],"prefix":"10.1016","volume":"270","author":[{"given":"Mohaddese","family":"Hajinezhadian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samira","family":"Ghaedmohammadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Hossein","family":"Nowroozzadeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amir","family":"Khosravi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Garshasb","family":"Rigi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9508-2632","authenticated-orcid":false,"given":"Fatemeh","family":"Sanie-Jahromi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.exer.2026.111123_bib1","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1128\/CMR.15.1.111-124.2002","article-title":"Bacterial endophthalmitis: epidemiology, therapeutics, and bacterium-host interactions","volume":"15","author":"Callegan","year":"2002","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.exer.2026.111123_bib2","doi-asserted-by":"crossref","first-page":"1971","DOI":"10.1167\/iovs.07-1377","article-title":"Eubacterial PCR for bacterial detection and identification in 100 acute postcataract surgery endophthalmitis","volume":"49","author":"Chiquet","year":"2008","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"10.1016\/j.exer.2026.111123_bib3","article-title":"Staphylococcus aureus alpha-toxin contributes to increased incidence in a murine model of experimental endogenous bacterial endophthalmitis","volume":"64","author":"Coburn","year":"2023","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"10.1016\/j.exer.2026.111123_bib4","first-page":"301","article-title":"Comparison of blood culture and multiplex real-time PCR for the diagnosis of nosocomial sepsis","volume":"82","author":"Dinc","year":"2016","journal-title":"Minerva Anestesiol."},{"key":"10.1016\/j.exer.2026.111123_bib5","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1128\/CMR.00113-16","article-title":"Bacterial and fungal endophthalmitis","volume":"30","author":"Durand","year":"2017","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.exer.2026.111123_bib6","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/S0161-6420(80)35241-X","article-title":"Management of infectious endophthalmitis","volume":"87","author":"Forster","year":"1980","journal-title":"Ophthalmology"},{"key":"10.1016\/j.exer.2026.111123_bib7","article-title":"A SYBR green based multiplex real-time PCR assay for rapid detection and differentiation of ocular bacterial pathogens","volume":"171","author":"Geetha","year":"2020","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.exer.2026.111123_bib8","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2024.1363437","article-title":"Spectrum and antibiotic sensitivity of bacterial keratitis: a retrospective analysis of eight years in a tertiary referral hospital in Southwest China","volume":"14","author":"Guo","year":"2024","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.exer.2026.111123_bib9","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.ajo.2019.11.026","article-title":"Specific PCR and quantitative real-time PCR in ocular samples from acute and delayed-onset postoperative endophthalmitis","volume":"212","author":"Kosacki","year":"2020","journal-title":"Am. J. Ophthalmol."},{"key":"10.1016\/j.exer.2026.111123_bib10","first-page":"10","article-title":"Practical guidance for clinical microbiology laboratories: diagnosis of ocular infections","volume":"34","author":"Leal Jr","year":"2021","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.exer.2026.111123_bib11","doi-asserted-by":"crossref","DOI":"10.1155\/2022\/1265556","article-title":"Endophthalmitis caused by Pseudomonas aeruginosa: clinical characteristics, outcomes, and antibiotics sensitivities","volume":"2022","author":"Lin","year":"2022","journal-title":"Journal of Ophthalmology"},{"key":"10.1016\/j.exer.2026.111123_bib12","doi-asserted-by":"crossref","first-page":"3005","DOI":"10.2147\/IDR.S367222","article-title":"Ten-year analysis of pathogenic factors and etiological characteristics of endophthalmitis from a tertiary eye center in North China","author":"Liu","year":"2022","journal-title":"Infect. Drug Resist."},{"key":"10.1016\/j.exer.2026.111123_bib13","doi-asserted-by":"crossref","first-page":"3509","DOI":"10.1007\/s00216-024-05301-0","article-title":"A dual-mode platform for the rapid detection of Escherichia coli O157: H7 based on CRISPR\/Cas12a and RPA","volume":"416","author":"Luo","year":"2024","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.exer.2026.111123_bib14","doi-asserted-by":"crossref","first-page":"697","DOI":"10.3109\/02713683.2014.957327","article-title":"DNA extraction methods for panbacterial and panfungal PCR detection in intraocular fluids","volume":"40","author":"Mazoteras","year":"2015","journal-title":"Curr. Eye Res."},{"key":"10.1016\/j.exer.2026.111123_bib15","doi-asserted-by":"crossref","first-page":"446","DOI":"10.3928\/15428877-20071101-01","article-title":"Endophthalmitis caused by Staphylococcus Epidermidis: in vitro antibiotic susceptibilities and clinical outcomes","volume":"38","author":"Miller","year":"2007","journal-title":"Ophthalmic Surgery, Lasers and Imaging Retina"},{"key":"10.1016\/j.exer.2026.111123_bib16","article-title":"Clinical characteristics of Staphylococcus Epidermidis: a systematic review","volume":"9","author":"Namvar","year":"2014","journal-title":"GMS hygiene and infection control"},{"key":"10.1016\/j.exer.2026.111123_bib17","doi-asserted-by":"crossref","first-page":"141","DOI":"10.2174\/1874364100802010141","article-title":"Polymerase chain reaction in intraocular inflammation","volume":"2","author":"Nandi","year":"2008","journal-title":"Open Ophthalmol. J."},{"key":"10.1016\/j.exer.2026.111123_bib18","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1076\/0927-3948(200009)831-KFT189","article-title":"Use of PCR in endophthalmitis","volume":"8","author":"Okhravi","year":"2000","journal-title":"Ocul. Immunol. Inflamm."},{"key":"10.1016\/j.exer.2026.111123_bib19","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1136\/bjo.81.9.719","article-title":"Assessment of a standard treatment protocol on visual outcome following presumed bacterial endophthalmitis","volume":"81","author":"Okhravi","year":"1997","journal-title":"British journal of ophthalmology"},{"key":"10.1016\/j.exer.2026.111123_bib20","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1542\/pir.2017-0224","article-title":"Staphylococcus aureus","volume":"39","author":"Ondusko","year":"2018","journal-title":"Pediatr. Rev."},{"key":"10.1016\/j.exer.2026.111123_bib21","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1186\/s12879-017-2264-5","article-title":"Comparison of methods for identifying causative bacterial microorganisms in presumed acute endophthalmitis: conventional culture, blood culture, and PCR","volume":"17","author":"Pongsachareonnont","year":"2017","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.exer.2026.111123_bib22","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1038\/eye.2008.197","article-title":"Microbiological profile of culture-proven cases of exogenous and endogenous endophthalmitis: a 10-year retrospective study","volume":"23","author":"Ramakrishnan","year":"2009","journal-title":"Eye"},{"key":"10.1016\/j.exer.2026.111123_bib23","series-title":"A Review of Molecular Methods Methods Used for Epidemiological Studies of Illogical Agents","author":"Ranjbar","year":"2003"},{"key":"10.1016\/j.exer.2026.111123_bib24","article-title":"Methods of detection of human pathogenic mycobacteria-molecular approach","volume":"41","author":"Roshdi Maleki","year":"2023","journal-title":"Journal of Medical Council of Iran"},{"key":"10.1016\/j.exer.2026.111123_bib25","doi-asserted-by":"crossref","DOI":"10.4414\/smw.2014.13911","article-title":"Incremental value of multiplex real-time PCR for the early diagnosis of sepsis in the emergency department","volume":"144","author":"Schaub","year":"2014","journal-title":"Swiss Med. Wkly."},{"key":"10.1016\/j.exer.2026.111123_bib26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2415-13-41","article-title":"Diagnostic techniques for inflammatory eye disease: past, present and future: a review","volume":"13","author":"Teoh","year":"2013","journal-title":"BMC Ophthalmol."},{"key":"10.1016\/j.exer.2026.111123_bib27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12886-017-0612-2","article-title":"Bacterial profile of ocular infections: a systematic review","volume":"17","author":"Teweldemedhin","year":"2017","journal-title":"BMC Ophthalmol."},{"key":"10.1016\/j.exer.2026.111123_bib28","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.ajo.2016.11.006","article-title":"Endophthalmitis after clear corneal cataract surgery: outcomes over two decades","volume":"174","author":"Yannuzzi","year":"2017","journal-title":"American journal of ophthalmology"},{"key":"10.1016\/j.exer.2026.111123_bib29","doi-asserted-by":"crossref","DOI":"10.1002\/jcla.24686","article-title":"Developing a multiplex PCR\u2010based assay kit for blood stream infection by analyzing genomic big data","volume":"36","author":"Zhang","year":"2022","journal-title":"J. Clin. Lab. Anal."}],"container-title":["Experimental Eye Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483526002794?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483526002794?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T01:30:29Z","timestamp":1785893429000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014483526002794"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":29,"alternative-id":["S0014483526002794"],"URL":"https:\/\/doi.org\/10.1016\/j.exer.2026.111123","relation":{},"ISSN":["0014-4835"],"issn-type":[{"value":"0014-4835","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development and evaluation of a multi-panel real-time PCR assay for rapid detection of six bacterial species causing endophthalmitis","name":"articletitle","label":"Article Title"},{"value":"Experimental Eye Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exer.2026.111123","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"111123"}}