{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T08:50:56Z","timestamp":1791363056975,"version":"4.3.1"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2014\/50928-2"],"award-info":[{"award-number":["2014\/50928-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["RED-00053-21"],"award-info":[{"award-number":["RED-00053-21"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ-04449-22"],"award-info":[{"award-number":["APQ-04449-22"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["303547\/2023-5"],"award-info":[{"award-number":["303547\/2023-5"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["465687\/2014-8"],"award-info":[{"award-number":["465687\/2014-8"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Eye Research"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.exer.2026.111205","type":"journal-article","created":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T21:56:22Z","timestamp":1786917382000},"page":"111205","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Investigating the therapeutic role of Leuculectin, a C-type lectin from Bothrops leucurus snake venom, in corneal neovascularization"],"prefix":"10.1016","volume":"272","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5287-455X","authenticated-orcid":false,"given":"Lays Fernanda Nunes","family":"Dourado","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1382-2487","authenticated-orcid":false,"given":"Carolina Nunes","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9378-0980","authenticated-orcid":false,"given":"Carolina Meireles Bastos","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2845-0447","authenticated-orcid":false,"given":"Eladio Flores","family":"Sanchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2784-5658","authenticated-orcid":false,"given":"Luciana Souza","family":"de Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9148-8915","authenticated-orcid":false,"given":"Catarina","family":"Pacheco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5763-7553","authenticated-orcid":false,"given":"Bruno","family":"Sarmento","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3878-5192","authenticated-orcid":false,"given":"Marina Fran\u00e7a","family":"Dias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8068-5211","authenticated-orcid":false,"given":"Silvia Lig\u00f3rio","family":"Fialho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.exer.2026.111205_bib1","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/j.ijbiomac.2018.08.064","article-title":"In vitro effect of Bothrops leucurus lectin (BLL) against Leishmania amazonensis and Leishmania braziliensis infection","volume":"120","author":"Aranda-Souza","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.exer.2026.111205_bib2","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.toxicon.2014.02.018","article-title":"A lectin from Bothrops leucurus snake venom raises cytosolic calcium levels and promotes B16-F10 melanoma necrotic cell death via mitochondrial permeability transition","volume":"82","author":"Aranda-Souza","year":"2014","journal-title":"Toxicon"},{"key":"10.1016\/j.exer.2026.111205_bib3","doi-asserted-by":"crossref","DOI":"10.1016\/j.tiv.2021.105150","article-title":"Comparative evaluation of HET-CAM and ICE methods for objective assessment of ocular irritation caused by selected pesticide products","volume":"74","author":"Budai","year":"2021","journal-title":"Toxicol. Vitro"},{"key":"10.1016\/j.exer.2026.111205_bib4","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.ijbiomac.2017.04.012","article-title":"Angiogenenic effects of BpLec, a C-type lectin isolated from Bothrops pauloensis snake venom","volume":"102","author":"Castanheira","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.exer.2026.111205_bib5","doi-asserted-by":"crossref","first-page":"2105","DOI":"10.1182\/blood-2003-07-2483","article-title":"Aggretin, a snake venom-derived endothelial integrin alpha 2 beta 1 agonist, induces angiogenesis via expression of vascular endothelial growth factor","volume":"103","author":"Chung","year":"2004","journal-title":"Blood"},{"key":"10.1016\/j.exer.2026.111205_bib6","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1111\/j.1538-7836.2008.03233.x","article-title":"Registry of exogenous hemostatic factors of, S.S.C.o.t.I., 2009. Scientific and standardization committee communications: classification and nomenclature of snake venom C-type lectins and related proteins","volume":"7","author":"Clemetson","year":"2009","journal-title":"J. Thromb. Haemostasis"},{"key":"10.1016\/j.exer.2026.111205_bib7","doi-asserted-by":"crossref","DOI":"10.1016\/j.exer.2021.108457","article-title":"Corneal angiogenic privilege and its failure","volume":"204","author":"Di Zazzo","year":"2021","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2026.111205_bib8","doi-asserted-by":"crossref","DOI":"10.1016\/j.xops.2024.100492","article-title":"Suppression of neovascularization by topical and subconjunctival Bevacizumab after high-risk corneal transplantation","volume":"4","author":"Dohlman","year":"2024","journal-title":"Ophthalmol Sci."},{"key":"10.1016\/j.exer.2026.111205_bib9","doi-asserted-by":"crossref","DOI":"10.3390\/ijms25105479","article-title":"Available therapeutic options for corneal neovascularization: a review","volume":"25","author":"Drzyzga","year":"2024","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.exer.2026.111205_bib10","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1007\/s10456-023-09876-7","article-title":"Pathological angiogenesis: mechanisms and therapeutic strategies","volume":"26","author":"Dudley","year":"2023","journal-title":"Angiogenesis"},{"key":"10.1016\/j.exer.2026.111205_bib11","doi-asserted-by":"crossref","DOI":"10.3390\/toxins11030136","article-title":"Structurally robust and functionally highly Versatile-C-Type lectin (-Related) proteins in snake venoms","volume":"11","author":"Eble","year":"2019","journal-title":"Toxins (Basel)"},{"key":"10.1016\/j.exer.2026.111205_bib12","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/s40662-017-0094-6","article-title":"Therapeutic approaches for corneal neovascularization","volume":"4","author":"Feizi","year":"2017","journal-title":"Eye Vis (Lond)"},{"key":"10.1016\/j.exer.2026.111205_bib13","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.2174\/1389450121666200319111710","article-title":"Anti-VEGF treatment in corneal diseases","volume":"21","author":"Giannaccare","year":"2020","journal-title":"Curr. Drug Targets"},{"key":"10.1016\/j.exer.2026.111205_bib14","series-title":"In Vitro Test Methods Proposed for Identifying Eye Injury Hazard Potential of Chemicals and Products","first-page":"B31","article-title":"ICCVAM-recommended test method protocol: hen's egg test \u2013 chorioallantoic membrane (HET-CAM) test method, ICCVAM test method evaluation report: current validation status of","year":"2010"},{"key":"10.1016\/j.exer.2026.111205_bib15","doi-asserted-by":"crossref","first-page":"349","DOI":"10.4142\/jvs.2015.16.3.349","article-title":"Air assisted lamellar keratectomy for the corneal haze model","volume":"16","author":"Kim","year":"2015","journal-title":"J. Vet. Sci."},{"key":"10.1016\/j.exer.2026.111205_bib16","doi-asserted-by":"crossref","first-page":"335","DOI":"10.4161\/cc.7.3.5234","article-title":"Integrin affinity modulation in angiogenesis","volume":"7","author":"Mahabeleshwar","year":"2008","journal-title":"Cell Cycle"},{"key":"10.1016\/j.exer.2026.111205_bib17","doi-asserted-by":"crossref","DOI":"10.3390\/ph14101042","article-title":"Ten-year outcomes of intravitreal Bevacizumab for myopic choroidal neovascularization: analysis of prognostic factors","volume":"14","author":"Mallone","year":"2021","journal-title":"Pharmaceuticals (Basel)"},{"key":"10.1016\/j.exer.2026.111205_bib18","doi-asserted-by":"crossref","first-page":"862","DOI":"10.3390\/toxins4100862","article-title":"Vixapatin (VP12), a c-type lectin-protein from Vipera xantina palestinae venom: characterization as a novel anti-angiogenic compound","volume":"4","author":"Momic","year":"2012","journal-title":"Toxins (Basel)"},{"key":"10.1016\/j.exer.2026.111205_bib19","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1096\/fj.201600351R","article-title":"Lebecetin, a C-type lectin, inhibits choroidal and retinal neovascularization","volume":"31","author":"Montassar","year":"2017","journal-title":"FASEB J."},{"key":"10.1016\/j.exer.2026.111205_bib20","doi-asserted-by":"crossref","DOI":"10.1016\/j.exer.2020.108363","article-title":"Corneal neovascularization","volume":"202","author":"Nicholas","year":"2021","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.exer.2026.111205_bib21","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.cbpb.2011.02.001","article-title":"Purification of a lectin with antibacterial activity from Bothrops leucurus snake venom","volume":"159","author":"Nunes Edos","year":"2011","journal-title":"Comp. Biochem. Physiol. B Biochem. Mol. Biol."},{"key":"10.1016\/j.exer.2026.111205_bib22","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.ijbiomac.2020.01.033","article-title":"Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma","volume":"148","author":"Petrovic","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.exer.2026.111205_bib23","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1002\/jcp.20935","article-title":"Lebectin, a Macrovipera lebetina venom-derived C-type lectin, inhibits angiogenesis both in vitro and in vivo","volume":"211","author":"Pilorget","year":"2007","journal-title":"J. Cell. Physiol."},{"key":"10.1016\/j.exer.2026.111205_bib24","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1586\/eop.13.8","article-title":"Corneal neovascularization: a review of the molecular biology and current therapies","volume":"8","author":"Rolfsen","year":"2013","journal-title":"Expert Rev. Ophthalmol."},{"key":"10.1016\/j.exer.2026.111205_bib25","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.biochi.2026.05.013","article-title":"Neuwlectin, a novel C-type lectin from Bothrops neuwiedi venom, inhibits platelet aggregation induced by cancer cells and angiogenesis","volume":"247","author":"Rosa","year":"2026","journal-title":"Biochimie"},{"key":"10.1016\/j.exer.2026.111205_bib26","doi-asserted-by":"crossref","first-page":"4491","DOI":"10.1007\/s11033-023-08355-y","article-title":"Inhibitory potency of the nettle lectin on neovascularization: a biomolecule for carbohydrate-mediated targeting of angiogenesis","volume":"50","author":"Samadian","year":"2023","journal-title":"Mol. Biol. Rep."},{"key":"10.1016\/j.exer.2026.111205_bib27","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.matbio.2007.01.001","article-title":"Lebectin and lebecetin, two C-type lectins from snake venom, inhibit alpha5beta1 and alphaV-containing integrins","volume":"26","author":"Sarray","year":"2007","journal-title":"Matrix Biol."},{"key":"10.1016\/j.exer.2026.111205_bib28","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/s40409-015-0038-3","article-title":"Snake venom galactoside-binding lectins: a structural and functional overview","volume":"21","author":"Sartim","year":"2015","journal-title":"J. Venom. Anim. Toxins Incl. Trop. Dis."},{"key":"10.1016\/j.exer.2026.111205_bib29","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1167\/tvst.10.8.32","article-title":"Effect of Bevacizumab on the viability and metabolism of human corneal epithelial and endothelial cells: an in vitro study","volume":"10","author":"Shokoohi","year":"2021","journal-title":"Transl. Vis. Sci. Technol."},{"key":"10.1016\/j.exer.2026.111205_bib30","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.jtos.2012.01.005","article-title":"Corneal neovascularization and the utility of topical VEGF inhibition: ranibizumab (Lucentis) vs bevacizumab (Avastin)","volume":"10","author":"Stevenson","year":"2012","journal-title":"Ocul. Surf."},{"key":"10.1016\/j.exer.2026.111205_bib31","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1016\/j.bbrc.2008.02.091","article-title":"Agglucetin, a tetrameric C-type lectin-like venom protein, regulates endothelial cell survival and promotes angiogenesis by activating integrin alphavbeta3 signaling","volume":"369","author":"Wang","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.exer.2026.111205_bib32","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1186\/s12951-024-02510-8","article-title":"A SU6668 pure nanoparticle-based eyedrops: toward its high drug accumulation and long-time treatment for corneal neovascularization","volume":"22","author":"Wu","year":"2024","journal-title":"J. Nanobiotechnol."},{"key":"10.1016\/j.exer.2026.111205_bib33","doi-asserted-by":"crossref","DOI":"10.1016\/j.bj.2023.03.005","article-title":"Targeted dexamethasone nano-prodrug for corneal neovascularization management","volume":"47","author":"Yin","year":"2024","journal-title":"Biomed. J."},{"key":"10.1016\/j.exer.2026.111205_bib34","first-page":"955","article-title":"Expression of integrins and MMPs during alkaline-burn-induced corneal angiogenesis","volume":"43","author":"Zhang","year":"2002","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.exer.2026.111205_bib35","doi-asserted-by":"crossref","DOI":"10.1136\/bmjopen-2020-040906","article-title":"Comparative efficacy and safety of different regimens of ranibizumab for neovascular age-related macular degeneration: a network meta-analysis of randomised controlled trials","volume":"11","author":"Zhao","year":"2021","journal-title":"BMJ Open"}],"container-title":["Experimental Eye Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483526003611?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014483526003611?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T08:04:58Z","timestamp":1791360298000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014483526003611"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":35,"alternative-id":["S0014483526003611"],"URL":"https:\/\/doi.org\/10.1016\/j.exer.2026.111205","relation":{},"ISSN":["0014-4835"],"issn-type":[{"value":"0014-4835","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Investigating the therapeutic role of Leuculectin, a C-type lectin from Bothrops leucurus snake venom, in corneal neovascularization","name":"articletitle","label":"Article Title"},{"value":"Experimental Eye Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exer.2026.111205","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"111205"}}