{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T01:01:21Z","timestamp":1780275681773,"version":"3.54.0"},"reference-count":54,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":547,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","exphem.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Hematology"],"published-print":{"date-parts":[[2024,12]]},"DOI":"10.1016\/j.exphem.2024.104652","type":"journal-article","created":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T19:22:09Z","timestamp":1727810529000},"page":"104652","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["A new era of functional experimentation in human hematopoiesis and leukemia research"],"prefix":"10.1016","volume":"140","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7454-6282","authenticated-orcid":false,"given":"Thomas","family":"K\u00f6hnke","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravindra","family":"Majeti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.exphem.2024.104652_bib0001","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1002\/jcp.1030620313","article-title":"The distribution of colony-forming cells among spleen colonies","volume":"62","author":"Siminovitch","year":"1963","journal-title":"J Cell Comp Physiol"},{"key":"10.1016\/j.exphem.2024.104652_bib0002","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1038\/197452a0","article-title":"Cytological demonstration of the clonal nature of spleen colonies derived from transplanted mouse marrow cells","volume":"197","author":"Becker","year":"1963","journal-title":"Nature"},{"key":"10.1016\/j.exphem.2024.104652_bib0003","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1038\/nm1005-1026","article-title":"Perspectives on the properties of stem cells","volume":"11","author":"McCulloch","year":"2005","journal-title":"Nat Med"},{"key":"10.1016\/j.exphem.2024.104652_bib0004","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1126\/science.144.3620.844","article-title":"Spleen-colony formation in anemic mice of genotype Ww","volume":"144","author":"McCulloch","year":"1964","journal-title":"Science"},{"key":"10.1016\/j.exphem.2024.104652_bib0005","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1073\/pnas.51.1.29","article-title":"A stochastic model of stem cell proliferation, based on the growth of spleen colony-forming cells","volume":"51","author":"Till","year":"1964","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.exphem.2024.104652_bib0006","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/1074-7613(94)90037-X","article-title":"The long-term repopulating subset of hematopoietic stem cells is deterministic and isolatable by phenotype","volume":"1","author":"Morrison","year":"1994","journal-title":"Immunity"},{"key":"10.1016\/j.exphem.2024.104652_bib0007","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1126\/science.2898810","article-title":"Purification and characterization of mouse hematopoietic stem cells","volume":"241","author":"Spangrude","year":"1988","journal-title":"Science"},{"key":"10.1016\/j.exphem.2024.104652_bib0008","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1038\/347188a0","article-title":"Separation of pluripotent haematopoietic stem cells from spleen colony-forming cells","volume":"347","author":"Jones","year":"1990","journal-title":"Nature"},{"key":"10.1016\/j.exphem.2024.104652_bib0009","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1084\/jem.175.1.175","article-title":"Searching for hematopoietic stem cells: evidence that Thy-1.1lo Lin- Sca-1+ cells are the only stem cells in C57BL\/Ka-Thy-1.1 bone marrow","volume":"175","author":"Uchida","year":"1992","journal-title":"J Exp Med"},{"key":"10.1016\/j.exphem.2024.104652_bib0010","doi-asserted-by":"crossref","first-page":"2804","DOI":"10.1073\/pnas.89.7.2804","article-title":"Isolation of a candidate human hematopoietic stem-cell population","volume":"89","author":"Baum","year":"1992","journal-title":"Proc Natl Acad Sci U S A."},{"key":"10.1016\/j.exphem.2024.104652_bib0011","doi-asserted-by":"crossref","first-page":"4793","DOI":"10.1182\/blood-2008-08-077941","article-title":"Stem cell concepts renew cancer research","volume":"112","author":"Dick","year":"2008","journal-title":"Blood"},{"key":"10.1016\/j.exphem.2024.104652_bib0012","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1182\/blood-2016-10-696054","article-title":"Biology and relevance of human acute myeloid leukemia stem cells","volume":"129","author":"Thomas","year":"2017","journal-title":"Blood"},{"key":"10.1016\/j.exphem.2024.104652_bib0013","first-page":"125","article-title":"Experimental evaluation of potential anticancer agents. XXI. Scheduling of arabinosylcytosine to take advantage of its S-phase specificity against leukemia cells","volume":"51","author":"Skipper","year":"1967","journal-title":"Cancer Chemother Rep"},{"key":"10.1016\/j.exphem.2024.104652_bib0014","doi-asserted-by":"crossref","DOI":"10.3389\/fonc.2022.854973","article-title":"Murine models of acute myeloid leukemia","volume":"12","author":"Kurtz","year":"2022","journal-title":"Front Oncol"},{"key":"10.1016\/j.exphem.2024.104652_bib0015","first-page":"20","article-title":"Murine models of acute myeloid leukaemia","author":"Almosailleakh","year":"2019","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.exphem.2024.104652_bib0016","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.cell.2005.09.025","article-title":"The menin tumor suppressor protein is an essential oncogenic cofactor for MLL-associated leukemogenesis","volume":"123","author":"Yokoyama","year":"2005","journal-title":"Cell"},{"key":"10.1016\/j.exphem.2024.104652_bib0017","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1038\/nchembio.773","article-title":"Menin-MLL inhibitors reverse oncogenic activity of MLL fusion proteins in leukemia","volume":"8","author":"Grembecka","year":"2012","journal-title":"Nat Chem Biol"},{"key":"10.1016\/j.exphem.2024.104652_bib0018","article-title":"Menin inhibition with revumenib for kmt2a-rearranged relapsed or refractory acute leukemia (AUGMENT-101)","author":"Issa","year":"2024","journal-title":"J Clin Oncol"},{"key":"10.1016\/j.exphem.2024.104652_bib0019","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1038\/s41586-023-05806-1","article-title":"Aberrant activation of TCL1A promotes stem cell expansion in clonal haematopoiesis","volume":"616","author":"Weinstock","year":"2023","journal-title":"Nature"},{"key":"10.1016\/j.exphem.2024.104652_bib0020","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1038\/s41588-022-01121-z","article-title":"Genome-wide analyses of 200,453 individuals yields new insights into the causes and consequences of clonal hematopoiesis","volume":"54","author":"Kar","year":"2022","journal-title":"Nat Genet"},{"key":"10.1016\/j.exphem.2024.104652_bib0021","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.93019","article-title":"Convergent epigenetic evolution drives relapse in acute myeloid leukemia","volume":"13","author":"Nuno","year":"2024","journal-title":"Elife"},{"key":"10.1016\/j.exphem.2024.104652_bib0022","doi-asserted-by":"crossref","first-page":"18","DOI":"10.7554\/eLife.27873","article-title":"Multiplexed genetic engineering of human hematopoietic stem and progenitor cells using CRISPR\/Cas9 and AAV6","volume":"6","author":"Bak","year":"2017","journal-title":"Elife"},{"key":"10.1016\/j.exphem.2024.104652_bib0023","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/j.stem.2017.07.015","article-title":"Multiplex CRISPR\/Cas9-based genome editing in human hematopoietic stem cells models clonal hematopoiesis and myeloid neoplasia","volume":"21","author":"Tothova","year":"2017","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.exphem.2024.104652_bib0024","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1158\/2643-3230.BCD-23-0195","article-title":"IDH1-mutant preleukemic hematopoietic stem cells can be eliminated by inhibition of oxidative phosphorylation","volume":"5","author":"Landberg","year":"2024","journal-title":"Blood Cancer Discov"},{"key":"10.1016\/j.exphem.2024.104652_bib0025","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1158\/2643-3230.BCD-21-0143","article-title":"The cell type\u2013specific 5hmC landscape and dynamics of healthy human hematopoiesis and TET2-mutant preleukemia","volume":"3","author":"Nakauchi","year":"2022","journal-title":"Blood Cancer Discov"},{"key":"10.1016\/j.exphem.2024.104652_bib0026","first-page":"133","article-title":"RUNX1 loss renders hematopoietic and leukemic cells dependent on IL-3 and sensitive to JAK inhibition","author":"Fan","year":"2023","journal-title":"J Clin Invest"},{"key":"10.1016\/j.exphem.2024.104652_bib0027","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1158\/2643-3230.BCD-23-0235","article-title":"Human ASXL1-mutant hematopoiesis is driven by a truncated protein associated with aberrant deubiquitination of H2AK119","volume":"5","author":"Kohnke","year":"2024","journal-title":"Blood Cancer Discov"},{"key":"10.1016\/j.exphem.2024.104652_bib0028","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1038\/nm.4103","article-title":"A humanized bone marrow ossicle xenotransplantation model enables improved engraftment of healthy and leukemic human hematopoietic cells","volume":"22","author":"Reinisch","year":"2016","journal-title":"Nat Med"},{"key":"10.1016\/j.exphem.2024.104652_bib0029","doi-asserted-by":"crossref","DOI":"10.3791\/65874","article-title":"Simplified intrafemoral injections using live mice allow for continuous bone marrow analysis","author":"Nakauchi","year":"2023","journal-title":"J Vis Exp"},{"key":"10.1016\/j.exphem.2024.104652_bib0030","first-page":"329","article-title":"Human AML-iPSCs reacquire leukemic properties after differentiation and model clonal variation of disease","volume":"20","author":"Chao","year":"2017","journal-title":"Stem Cells"},{"key":"10.1016\/j.exphem.2024.104652_bib0031","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1182\/blood-2023-186440","article-title":"Gene correction of DNMT3A:R882H in primary human aml demonstrates that this mutation is not required for disease maintenance, but is associated with increased leukemia stem cell frequency","volume":"142","author":"Koehnke","year":"2023","journal-title":"Blood"},{"key":"10.1016\/j.exphem.2024.104652_bib0032","doi-asserted-by":"crossref","first-page":"1164","DOI":"10.1158\/2159-8290.CD-21-0502","article-title":"Reprogramming cancer into antigen presenting cells as a novel immunotherapy","volume":"13","author":"Linde","year":"2023","journal-title":"Cancer Discov"},{"key":"10.1016\/j.exphem.2024.104652_bib0033","doi-asserted-by":"crossref","first-page":"4074","DOI":"10.1073\/pnas.1413383112","article-title":"Reprogramming of primary human Philadelphia chromosome-positive B cell acute lymphoblastic leukemia cells into nonleukemic macrophages","volume":"112","author":"McClellan","year":"2015","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.exphem.2024.104652_bib0034","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1158\/2159-8290.CD-22-0199","article-title":"Human bone marrow organoids for disease modeling, discovery, and validation of therapeutic targets in hematologic malignancies","volume":"13","author":"Khan","year":"2023","journal-title":"Cancer Discov"},{"key":"10.1016\/j.exphem.2024.104652_bib0035","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1038\/nbt.2858","article-title":"Development and function of human innate immune cells in a humanized mouse model","volume":"32","author":"Rongvaux","year":"2014","journal-title":"Nat Biotechnol"},{"key":"10.1016\/j.exphem.2024.104652_bib0036","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1016\/j.exphem.2015.04.001","article-title":"CD201 and CD27 identify hematopoietic stem and progenitor cells across multiple murine strains independently of Kit and Sca-1","volume":"43","author":"Vazquez","year":"2015","journal-title":"Exp Hematol"},{"key":"10.1016\/j.exphem.2024.104652_bib0037","doi-asserted-by":"crossref","first-page":"3327","DOI":"10.1182\/blood.V82.11.3327.3327","article-title":"Mouse strain variability in the expression of the hematopoietic stem cell antigen Ly-6A\/E by bone marrow cells","volume":"82","author":"Spangrude","year":"1993","journal-title":"Blood"},{"key":"10.1016\/j.exphem.2024.104652_bib0038","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.stemcr.2015.05.008","article-title":"The genetic landscape of hematopoietic stem cell frequency in mice","volume":"5","author":"Zhou","year":"2015","journal-title":"Stem Cell Rep"},{"key":"10.1016\/j.exphem.2024.104652_bib0039","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1038\/s41587-024-02349-2","article-title":"Improving the predictive power of mouse models","volume":"42","author":"Pera","year":"2024","journal-title":"Nat Biotechnol"},{"key":"10.1016\/j.exphem.2024.104652_bib0040","doi-asserted-by":"crossref","first-page":"2110","DOI":"10.1007\/s13346-022-01189-4","article-title":"Modelling acute myeloid leukemia (AML): What's new? A transition from the classical to the modern","volume":"13","author":"Dozzo","year":"2023","journal-title":"Drug Deliv Transl Res"},{"key":"10.1016\/j.exphem.2024.104652_bib0041","series-title":"The Leukemia-Lymphoma Cell Line Factsbook","author":"Drexler","year":"2001"},{"key":"10.1016\/j.exphem.2024.104652_bib0042","doi-asserted-by":"crossref","DOI":"10.1016\/j.drup.2020.100730","article-title":"Ex vivo cultures and drug testing of primary acute myeloid leukemia samples: Current techniques and implications for experimental design and outcome","volume":"53","author":"Cucchi","year":"2020","journal-title":"Drug Resist Updat"},{"key":"10.1016\/j.exphem.2024.104652_bib0043","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.ygeno.2016.04.003","article-title":"Changes of heterogeneous cell populations in the Ishikawa cell line during long-term culture: Proposal for an in vitro clonal evolution model of tumor cells","volume":"107","author":"Kasai","year":"2016","journal-title":"Genomics"},{"key":"10.1016\/j.exphem.2024.104652_bib0044","doi-asserted-by":"crossref","first-page":"2461","DOI":"10.1182\/blood-2015-01-561373","article-title":"Current challenges in clinical development of \"targeted therapies\": the case of acute myeloid leukemia","volume":"125","author":"Estey","year":"2015","journal-title":"Blood"},{"key":"10.1016\/j.exphem.2024.104652_bib0045","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1016\/j.cell.2017.06.010","article-title":"Defining a cancer dependency map","volume":"170","author":"Tsherniak","year":"2017","journal-title":"Cell"},{"key":"10.1016\/j.exphem.2024.104652_bib0046","doi-asserted-by":"crossref","first-page":"4520","DOI":"10.1016\/j.cell.2024.07.035","article-title":"Toward a foundation model of causal cell and tissue biology with a Perturbation Cell and Tissue Atlas","volume":"187","author":"Rood","year":"2024","journal-title":"Cell."},{"key":"10.1016\/j.exphem.2024.104652_bib0047","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1038\/nm0797-730","article-title":"Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell","volume":"3","author":"Bonnet","year":"1997","journal-title":"Nat Med"},{"key":"10.1016\/j.exphem.2024.104652_bib0048","doi-asserted-by":"crossref","first-page":"1976","DOI":"10.1182\/blood-2009-02-206565","article-title":"Leukemia-initiating cells from some acute myeloid leukemia patients with mutated nucleophosmin reside in the CD34(-) fraction","volume":"115","author":"Taussig","year":"2010","journal-title":"Blood"},{"key":"10.1016\/j.exphem.2024.104652_bib0049","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1038\/s41586-018-0497-0","article-title":"Population dynamics of normal human blood inferred from somatic mutations","volume":"561","author":"Lee-Six","year":"2018","journal-title":"Nature"},{"key":"10.1016\/j.exphem.2024.104652_bib0050","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1016\/j.cell.2019.01.022","article-title":"Lineage tracing in humans enabled by mitochondrial mutations and single-cell genomics","volume":"176","author":"Ludwig","year":"2019","journal-title":"Cell"},{"key":"10.1016\/j.exphem.2024.104652_bib0051","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1038\/nature13824","article-title":"Clonal dynamics of native haematopoiesis","volume":"514","author":"Sun","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.exphem.2024.104652_bib0052","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.stem.2023.12.001","article-title":"A time- and single-cell-resolved model of murine bone marrow hematopoiesis","volume":"31","author":"Kucinski","year":"2024","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.exphem.2024.104652_bib0053","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2317929121","article-title":"Clonal barcoding of endogenous adult hematopoietic stem cells reveals a spectrum of lineage contributions","volume":"121","author":"Feng","year":"2024","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.exphem.2024.104652_bib0054","doi-asserted-by":"crossref","first-page":"2184","DOI":"10.1038\/s41467-023-37167-8","article-title":"DRAG in situ barcoding reveals an increased number of HSPCs contributing to myelopoiesis with age","volume":"14","author":"Urbanus","year":"2023","journal-title":"Nat Commun"}],"container-title":["Experimental Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0301472X24005174?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0301472X24005174?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:37:35Z","timestamp":1780274255000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0301472X24005174"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12]]},"references-count":54,"alternative-id":["S0301472X24005174"],"URL":"https:\/\/doi.org\/10.1016\/j.exphem.2024.104652","relation":{},"ISSN":["0301-472X"],"issn-type":[{"value":"0301-472X","type":"print"}],"subject":[],"published":{"date-parts":[[2024,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A new era of functional experimentation in human hematopoiesis and leukemia research","name":"articletitle","label":"Article Title"},{"value":"Experimental Hematology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exphem.2024.104652","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 International Society for Experimental Hematology. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"104652"}}