{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T04:18:46Z","timestamp":1781756326166,"version":"3.54.5"},"reference-count":31,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100006668","name":"Fund for Scientific and Technological Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006668","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT 2017-1288"],"award-info":[{"award-number":["PICT 2017-1288"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT 2020-0297"],"award-info":[{"award-number":["PICT 2020-0297"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PUE 0055"],"award-info":[{"award-number":["PUE 0055"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008392","name":"Nelia and Amadeo Barletta Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","exphem.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Hematology"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1016\/j.exphem.2025.105288","type":"journal-article","created":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T02:24:35Z","timestamp":1762482275000},"page":"105288","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Cytoreductive treatment differentially affects platelet size and cytoskeletal megakaryocyte organization during thrombopoiesis in myeloproliferative neoplasms"],"prefix":"10.1016","volume":"153","author":[{"given":"Adela S.","family":"Cellucci","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danila B.","family":"Ya\u00f1uk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola R.","family":"Lev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana C.","family":"Glembotsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nora P.","family":"Goette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda C.","family":"Lira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9091-1240","authenticated-orcid":false,"given":"Geraldine","family":"De Luca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laureano J.","family":"Kamiya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula G.","family":"Heller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0403-9565","authenticated-orcid":false,"given":"Rosana F.","family":"Marta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.exphem.2025.105288_bib0001","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1182\/blood-2016-10-695957","article-title":"Diagnosis, risk stratification, and response evaluation in classical myeloproliferative neoplasms","volume":"129","author":"Rumi","year":"2017","journal-title":"Blood"},{"key":"10.1016\/j.exphem.2025.105288_bib0002","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1182\/blood-2016-10-695940","article-title":"Genetic basis and molecular pathophysiology of classical myeloproliferative neoplasms","volume":"129","author":"Vainchenker","year":"2017","journal-title":"Blood"},{"key":"10.1016\/j.exphem.2025.105288_bib0003","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1097\/MOH.0000000000000664","article-title":"Thrombosis in myeloproliferative neoplasms: update in pathophysiology","volume":"28","author":"Reeves","year":"2021","journal-title":"Curr Opin Hematol"},{"key":"10.1016\/j.exphem.2025.105288_bib0004","doi-asserted-by":"crossref","first-page":"3551","DOI":"10.3390\/cells10123551","article-title":"Progression of myeloproliferative neoplasms (MPN): diagnostic and therapeutic perspectives","volume":"10","author":"Baumeister","year":"2021","journal-title":"Cells"},{"key":"10.1016\/j.exphem.2025.105288_bib0005","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1038\/s41375-018-0077-1","article-title":"Philadelphia chromosome-negative classical myeloproliferative neoplasms: revised management recommendations from European LeukemiaNet","volume":"32","author":"Barbui","year":"2018","journal-title":"Leukemia"},{"key":"10.1016\/j.exphem.2025.105288_bib0006","first-page":"1","article-title":"Mechanism of action of hydroxyurea","volume":"19","author":"Yarbro","year":"1992","journal-title":"Semin Oncol"},{"key":"10.1016\/j.exphem.2025.105288_bib0007","doi-asserted-by":"crossref","first-page":"1931","DOI":"10.1182\/blood.V79.8.1931.1931","article-title":"Analysis of the mechanism of anagrelide-induced thrombocytopenia in humans","volume":"79","author":"Mazur","year":"1992","journal-title":"Blood"},{"key":"10.1016\/j.exphem.2025.105288_bib0008","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1111\/jth.12850","article-title":"Anagrelide platelet-lowering effect is due to inhibition of both megakaryocyte maturation and proplatelet formation: insight into potential mechanisms","volume":"13","author":"Espasandin","year":"2015","journal-title":"J Thromb Haemost"},{"key":"10.1016\/j.exphem.2025.105288_bib0009","doi-asserted-by":"crossref","first-page":"2093","DOI":"10.1182\/blood.V96.6.2093","article-title":"Interferon-\u03b1 directly represses megakaryopoiesis by inhibiting thrombopoietin-induced signaling through induction of SOCS-1","volume":"96","author":"Wang","year":"2000","journal-title":"Blood"},{"key":"10.1016\/j.exphem.2025.105288_bib0010","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1182\/blood-2007-12-125906","article-title":"Interferon-alpha 2b\u2013induced thrombocytopenia is caused by inhibition of platelet production but not proliferation and endomitosis in human megakaryocytes","volume":"112","author":"Yamane","year":"2008","journal-title":"Blood"},{"key":"10.1016\/j.exphem.2025.105288_bib0011","doi-asserted-by":"crossref","first-page":"3109","DOI":"10.1182\/blood-2009-04-214957","article-title":"Preclinical characterization of the selective JAK1\/2 inhibitor INCB018424: therapeutic implications for the treatment of myeloproliferative neoplasms","volume":"115","author":"Quinta\u2019s-Cardama","year":"2010","journal-title":"Blood"},{"key":"10.1016\/j.exphem.2025.105288_bib0012","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1046\/j.1365-2141.1999.01234.x","article-title":"Studies of platelet volume, chemistry and function in patients with essential thrombocythaemia treated with anagrelide","volume":"104","author":"Bellucci","year":"1999","journal-title":"Br J Haematol"},{"key":"10.1016\/j.exphem.2025.105288_bib0013","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1055\/s-0041-1731717","article-title":"Megakaryocyte cytoskeletal proteins in platelet biogenesis and diseases","volume":"122","author":"Mbiandjeu","year":"2022","journal-title":"Thromb Haemost"},{"key":"10.1016\/j.exphem.2025.105288_bib0014","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.jstrokecerebrovasdis.2011.09.009","article-title":"Mean platelet volume in acute phase of ischemic stroke, as predictor of mortality and functional outcome after 1 year","volume":"22","author":"Ar\u00e9valo-Lorido","year":"2013","journal-title":"J Stroke Cerebrovasc Dis"},{"key":"10.1016\/j.exphem.2025.105288_bib0015","doi-asserted-by":"crossref","first-page":"2391","DOI":"10.1182\/blood-2016-03-643544","article-title":"The 2016 revision to the World Health Organization classification of myeloid neoplasms and acute leukemia","volume":"127","author":"Arber","year":"2016","journal-title":"Blood"},{"key":"10.1016\/j.exphem.2025.105288_bib0016","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1038\/sj.leu.2404914","article-title":"Proposed criteria for the diagnosis of post-polycythemia vera and post-essential thrombocythemia myelofibrosis: a consensus statement from the International Working Group for Myelofibrosis Research and Treatment","volume":"22","author":"Barosi","year":"2008","journal-title":"Leukemia"},{"key":"10.1016\/j.exphem.2025.105288_bib0017","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.1182\/blood-2009-09-245837","article-title":"A dynamic prognostic model to predict survival in primary myelofibrosis: a study by the IWG-MRT (International Working Group for Myeloproliferative Neoplasms Research and Treatment)","volume":"115","author":"Passamonti","year":"2010","journal-title":"Blood"},{"key":"10.1016\/j.exphem.2025.105288_bib0018","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1111\/jth.12959","article-title":"The gene expression signature of anagrelide provides an insight into its mechanism of action and uncovers new regulators of megakaryopoiesis","volume":"13","author":"Ahluwalia","year":"2015","journal-title":"J Thromb Haemost"},{"key":"10.1016\/j.exphem.2025.105288_bib0019","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1182\/blood-2015-05-644583","article-title":"CCL5 derived from platelets increases megakaryocyte proplatelet formation","volume":"127","author":"Machlus","year":"2016","journal-title":"Blood"},{"key":"10.1016\/j.exphem.2025.105288_bib0020","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1111\/bjh.12349","article-title":"Platelet size for distinguishing between inherited thrombocytopenias and immune thrombocytopenia: a multicentric, real life study","volume":"162","author":"Noris","year":"2013","journal-title":"Br J Haematol"},{"key":"10.1016\/j.exphem.2025.105288_bib0021","doi-asserted-by":"crossref","first-page":"e268","DOI":"10.1097\/HS9.0000000000000268","article-title":"Anagrelide modulates proplatelet formation resulting in decreased number and increased size of platelets","volume":"3","author":"Miyashita","year":"2019","journal-title":"HemaSphere"},{"key":"10.1016\/j.exphem.2025.105288_bib0022","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1007\/s12185-014-1673-0","article-title":"Increased mean platelet volume and immature platelet fraction as potential predictors of thrombotic complications in BCR\/ABL negative myeloproliferative neoplasms","volume":"100","author":"Kissova","year":"2014","journal-title":"Int J Hematol"},{"key":"10.1016\/j.exphem.2025.105288_bib0023","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1097\/MBC.0000000000000717","article-title":"Mean platelet volume and risk of thrombotic and bleeding complications in patients with Philadelphia chromosome negative myeloproliferative neoplasms","volume":"29","author":"Krashin","year":"2018","journal-title":"Blood Coagul Fibrinolysis"},{"key":"10.1016\/j.exphem.2025.105288_bib0024","doi-asserted-by":"crossref","first-page":"4076","DOI":"10.1182\/blood-2005-06-2204","article-title":"Differential roles of microtubule assembly and sliding in proplatelet formation by megakaryocytes","volume":"106","author":"Patel","year":"2005","journal-title":"Blood"},{"key":"10.1016\/j.exphem.2025.105288_bib0025","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1038\/ncomms1838","article-title":"Microtubule and cortical forces determine platelet size during vascular platelet production","volume":"3","author":"Thon","year":"2012","journal-title":"Nat Commun"},{"key":"10.1016\/j.exphem.2025.105288_bib0026","doi-asserted-by":"crossref","first-page":"800","DOI":"10.3324\/haematol.2008.001032","article-title":"Intrinsic impaired proplatelet formation and microtubule coil assembly of megakaryocytes in a mouse model of Bernard\u2013Soulier syndrome","volume":"94","author":"Strassel","year":"2009","journal-title":"Haematologica"},{"key":"10.1016\/j.exphem.2025.105288_bib0027","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1111\/ejh.12252","article-title":"TUBB1 mutation disrupting microtubule assembly impairs proplatelet formation and results in congenital macrothrombocytopenia","volume":"92","author":"Kunishima","year":"2014","journal-title":"Eur J Haematol"},{"key":"10.1016\/j.exphem.2025.105288_bib0028","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1038\/s41598-018-19643-0","article-title":"CCR5\/CCL5 axis interaction promotes migratory and invasiveness of pancreatic cancer cells","volume":"8","author":"Singh","year":"2018","journal-title":"Sci Rep"},{"key":"10.1016\/j.exphem.2025.105288_bib0029","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/j.jprot.2009.11.003","article-title":"In vivo pharmaco-proteomic analysis of hydroxyurea induced changes in the sickle red blood cell membrane proteome","volume":"73","author":"Ghatpande","year":"2010","journal-title":"J Proteomics"},{"key":"10.1016\/j.exphem.2025.105288_bib0030","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1056\/NEJMoa043800","article-title":"Hydroxyurea compared with anagrelide in high-risk essential thrombocythemia","volume":"353","author":"Harrison","year":"2005","journal-title":"N Engl J Med"},{"key":"10.1016\/j.exphem.2025.105288_bib0031","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1007\/s10637-019-00812-5","article-title":"Autophagy inhibition potentiates ruxolitinib-induced apoptosis in JAK2V617F cells","volume":"38","author":"Machado-Neto","year":"2020","journal-title":"Invest New Drugs"}],"container-title":["Experimental Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0301472X25005776?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0301472X25005776?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T13:13:37Z","timestamp":1778505217000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0301472X25005776"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":31,"alternative-id":["S0301472X25005776"],"URL":"https:\/\/doi.org\/10.1016\/j.exphem.2025.105288","relation":{},"ISSN":["0301-472X"],"issn-type":[{"value":"0301-472X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Cytoreductive treatment differentially affects platelet size and cytoskeletal megakaryocyte organization during thrombopoiesis in myeloproliferative neoplasms","name":"articletitle","label":"Article Title"},{"value":"Experimental Hematology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exphem.2025.105288","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 International Society for Experimental Hematology. Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"105288"}}