{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:52:02Z","timestamp":1791305522884,"version":"4.3.1"},"reference-count":27,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:00:00Z","timestamp":1172707200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Parasitology"],"published-print":{"date-parts":[[2007,3]]},"DOI":"10.1016\/j.exppara.2006.09.010","type":"journal-article","created":{"date-parts":[[2006,10,26]],"date-time":"2006-10-26T07:16:59Z","timestamp":1161847019000},"page":"291-295","source":"Crossref","is-referenced-by-count":41,"title":["Clonorchis sinensis: Development and evaluation of a nested polymerase chain reaction (PCR) assay"],"prefix":"10.1016","volume":"115","author":[{"given":"Ammini","family":"Parvathi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Sanath Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Kenchanna Prakasha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jieyuan","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuenian","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Indrani","family":"Karunasagar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iddya","family":"Karunasagar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.exppara.2006.09.010_bib1","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: a new generation of protein database search programs","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Research"},{"key":"10.1016\/j.exppara.2006.09.010_bib2","first-page":"17","article-title":"Nucleotide sequence of mitochondrial Co1 and ribosomal ITS II genes of Opisthorchis viverrini in North East Thailand","volume":"32","author":"Ando","year":"2001","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"10.1016\/j.exppara.2006.09.010_bib3","first-page":"R9","article-title":"Progress in assessment of morbidity due to Clonorchis sinensis infection: a review of recent literature","volume":"91","author":"Chen","year":"1994","journal-title":"Tropical Disease Bulletin"},{"key":"10.1016\/j.exppara.2006.09.010_bib4","first-page":"3","article-title":"Species determination of eggs of opisthorchiid and heterophyid flukes using scanning electron microscopy","volume":"31","author":"Ditrich","year":"1990","journal-title":"Angewandte Parasitologie"},{"key":"10.1016\/j.exppara.2006.09.010_bib5","unstructured":"FAO, 2004. Assessment and management of seafood quality and safety. Fisheries technical paper, 444."},{"key":"10.1016\/j.exppara.2006.09.010_bib6","doi-asserted-by":"crossref","first-page":"468","DOI":"10.4269\/ajtmh.1998.59.468","article-title":"Development and laboratory evaluation of a polymerase chain reaction for monitoring Schistosoma mansoni infestation of water","volume":"59","author":"Hamburger","year":"1998","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"10.1016\/j.exppara.2006.09.010_bib7","doi-asserted-by":"crossref","first-page":"907","DOI":"10.4269\/ajtmh.2001.65.907","article-title":"Polymerase chain reaction assay based on a highly repeated sequence of Schistosoma haematobium: a potential tool for monitoring schistosome-infested water","volume":"65","author":"Hamburger","year":"2001","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"10.1016\/j.exppara.2006.09.010_bib8","doi-asserted-by":"crossref","first-page":"87","DOI":"10.3347\/kjp.1997.35.2.87","article-title":"Immunoblot patterns of clonorchiasis","volume":"35","author":"Hong","year":"1997","journal-title":"Korean Journal of Parasitology"},{"key":"10.1016\/j.exppara.2006.09.010_bib9","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/S0014-4894(02)00112-1","article-title":"Clonorchis sinensis: glutathione S-transferase as a serodiagnostic antigen for detecting IgG and IgE antibodies","volume":"101","author":"Hong","year":"2002","journal-title":"Experimental Parasitology"},{"key":"10.1016\/j.exppara.2006.09.010_bib10","series-title":"Molecular Biology: A Project Approach","author":"Karcher","year":"1995"},{"key":"10.1016\/j.exppara.2006.09.010_bib11","doi-asserted-by":"crossref","first-page":"171","DOI":"10.3347\/kjp.1984.22.2.171","article-title":"Comparative morphology of heterophyids and Clonorchis sinensis causing human infections in Korea","volume":"22","author":"Lee","year":"1984","journal-title":"Korean Journal of Parasitology"},{"key":"10.1016\/j.exppara.2006.09.010_bib12","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.exppara.2005.09.012","article-title":"Clonorchis sinensis and Opisthorchis viverrini: development of a mitochondrial-based multiplex PCR for their identification and discrimination","volume":"112","author":"Le","year":"2006","journal-title":"Experimental Parasitology"},{"key":"10.1016\/j.exppara.2006.09.010_bib13","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1097\/00007611-198707000-00030","article-title":"Clonorchiasis: an update","volume":"80","author":"Lin","year":"1987","journal-title":"The Southern Medical Journal"},{"key":"10.1016\/j.exppara.2006.09.010_bib14","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S1473-3099(04)01252-6","article-title":"Clonorchiasis: a key foodborne zoonosis in China","volume":"5","author":"Lun","year":"2005","journal-title":"Lancet Infectious Diseases"},{"key":"10.1016\/j.exppara.2006.09.010_bib15","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1590\/S0074-02762004000400013","article-title":"Detection of Lymnaea columella infection by Fasciola hepatica through multiplex-PCR","volume":"99","author":"Magalhaes","year":"2004","journal-title":"Mem\u00f3rias do Instituto Oswaldo Cruz"},{"key":"10.1016\/j.exppara.2006.09.010_bib16","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1128\/CDLI.11.2.411-416.2004","article-title":"Molecular expression of a cysteine proteinase of Clonorchis sinensis and its application to an enzyme-linked immunosorbent assay for immuno diagnosis of clonorchiasis","volume":"11","author":"Nagano","year":"2004","journal-title":"Clinical and Diagnostic Laboratory Immunology"},{"key":"10.1016\/j.exppara.2006.09.010_bib17","doi-asserted-by":"crossref","first-page":"2286","DOI":"10.1128\/AEM.61.6.2286-2291.1995","article-title":"Identification and phylogenetic analysis of toxigenic cyanobacteria by multiplex randomly amplified polymorphic DNA PCR","volume":"61","author":"Neilan","year":"1995","journal-title":"Applied and Environmental Microbiology"},{"issue":"Suppl. 2","key":"10.1016\/j.exppara.2006.09.010_bib18","first-page":"13","article-title":"Geographical variation of the liver fluke Clonorchis sinensis from Korea and China based on the karyotypes, zymodeme and DNA sequences","volume":"32","author":"Park","year":"2001","journal-title":"South East Asian Journal of Tropical Medicine and Public Health"},{"key":"10.1016\/j.exppara.2006.09.010_bib19","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1046\/j.1365-2621.1998.3320157.x","article-title":"Field investigations on epidemiology and control of fish-borne parasites in Korea","volume":"33","author":"Rim","year":"1998","journal-title":"International Journal of Science and Technology"},{"key":"10.1016\/j.exppara.2006.09.010_bib20","doi-asserted-by":"crossref","first-page":"748","DOI":"10.2307\/3283253","article-title":"Detection of Fasciola hepatica in infected intermediate hosts using RT-PCR","volume":"80","author":"Rognlie","year":"1994","journal-title":"Journal of Parasitology"},{"key":"10.1016\/j.exppara.2006.09.010_bib21","first-page":"631","article-title":"Eggshell morphology of the small eggs of human trematodes in Thailand","volume":"22","author":"Tesana","year":"1991","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"10.1016\/j.exppara.2006.09.010_bib22","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1046\/j.1365-3156.1999.00499.x","article-title":"Little effect of praziquantel or artemisinin on clonorchiasis in northern Vietnam. A pilot study","volume":"4","author":"Tinga","year":"1999","journal-title":"Tropical Medicine and International Health."},{"key":"10.1016\/j.exppara.2006.09.010_bib23","unstructured":"WHO (World Health Organization), 1995. Control of food borne trematode infections: report of a WHO study group. WHO, Geneva, Switzerland. WHO Technical Report Series No. 849."},{"key":"10.1016\/j.exppara.2006.09.010_bib24","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1017\/S0031182001007235","article-title":"Development of a PCR-based method for the detection of Opisthorchis viverrini in experimentally infected hamsters","volume":"122","author":"Wongratanacheewin","year":"2001","journal-title":"Parasitology"},{"key":"10.1016\/j.exppara.2006.09.010_bib25","doi-asserted-by":"crossref","first-page":"3879","DOI":"10.1128\/JCM.40.10.3879-3880.2002","article-title":"Detection of Opisthorchis viverrini in human stool specimens by PCR","volume":"40","author":"Wongratanacheewin","year":"2002","journal-title":"Journal of Clinical Microbiology"},{"key":"10.1016\/j.exppara.2006.09.010_bib26","doi-asserted-by":"crossref","first-page":"183","DOI":"10.3347\/kjp.1998.36.3.183","article-title":"Immunodiagnosis of clonorchiasis using a recombinant antigen","volume":"36","author":"Yong","year":"1998","journal-title":"Korean Journal of Parasitology"},{"key":"10.1016\/j.exppara.2006.09.010_bib27","doi-asserted-by":"crossref","first-page":"814","DOI":"10.1128\/CDLI.11.4.814-817.2004","article-title":"Evaluation of Clonorchis sinensis recombinant 7-kilodalton antigen for serodiagnosis of clonorchiasis","volume":"11","author":"Zhao","year":"2004","journal-title":"Clinical and Diagnostic Laboratory Immunology"}],"container-title":["Experimental Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489406002177?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489406002177?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,4,18]],"date-time":"2020-04-18T15:45:13Z","timestamp":1587224713000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014489406002177"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,3]]},"references-count":27,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2007,3]]}},"alternative-id":["S0014489406002177"],"URL":"https:\/\/doi.org\/10.1016\/j.exppara.2006.09.010","relation":{},"ISSN":["0014-4894"],"issn-type":[{"value":"0014-4894","type":"print"}],"subject":[],"published":{"date-parts":[[2007,3]]}}}