{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T17:38:50Z","timestamp":1760204330404,"version":"3.44.0"},"reference-count":44,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2009,2,1]],"date-time":"2009-02-01T00:00:00Z","timestamp":1233446400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2009,2,1]],"date-time":"2009-02-01T00:00:00Z","timestamp":1233446400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2009,2,1]],"date-time":"2009-02-01T00:00:00Z","timestamp":1233446400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2009,2,1]],"date-time":"2009-02-01T00:00:00Z","timestamp":1233446400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2009,2,1]],"date-time":"2009-02-01T00:00:00Z","timestamp":1233446400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2009,2,1]],"date-time":"2009-02-01T00:00:00Z","timestamp":1233446400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2009,2,1]],"date-time":"2009-02-01T00:00:00Z","timestamp":1233446400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Parasitology"],"published-print":{"date-parts":[[2009,2]]},"DOI":"10.1016\/j.exppara.2008.10.018","type":"journal-article","created":{"date-parts":[[2008,11,9]],"date-time":"2008-11-09T04:12:15Z","timestamp":1226203935000},"page":"137-143","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":8,"title":["Trypanosoma brucei brucei: Effects of ferrous iron and heme on ecto-nucleoside triphosphate diphosphohydrolase activity"],"prefix":"10.1016","volume":"121","author":[{"given":"Milane S.","family":"Leite","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel","family":"Thomaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Henrique M.","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro L.","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Roberto","family":"Meyer-Fernandes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.exppara.2008.10.018_bib1","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1126\/science.1112642","article-title":"The genome of the African trypanosome Trypanosoma brucei","volume":"309","author":"Berriman","year":"2005","journal-title":"Science"},{"key":"10.1016\/j.exppara.2008.10.018_bib111","first-page":"210","article-title":"Polymers for delivering peptides and proteins","volume":"51","author":"Burnham","year":"1994","journal-title":"American Journal of Hospital Pharmacy"},{"key":"10.1016\/j.exppara.2008.10.018_bib2","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/BF00420912","article-title":"Oxidative damage to sarcoplasmic reticulum Ca2+ pump induced by Fe2+\/H2O2\/ascorbato is not mediated by lipid peroxidation or thiol oxidation and leads to protein fragmentation","volume":"159","author":"Castilho","year":"1996","journal-title":"Molecular Cell Biochemistry"},{"key":"10.1016\/j.exppara.2008.10.018_bib3","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1172\/JCI110302","article-title":"Mechanism of hemolysis induced by ferriprotoporphyrin IX","volume":"68","author":"Chou","year":"1981","journal-title":"Journal of Clinical Investigation"},{"key":"10.1016\/j.exppara.2008.10.018_bib4","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/S0169-4758(96)10073-9","article-title":"Metabolic compartmentation in African trypanosomes","volume":"12","author":"Clayton","year":"1996","journal-title":"Parasitology Today"},{"key":"10.1016\/j.exppara.2008.10.018_bib5","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1016\/j.freeradbiomed.2004.02.008","article-title":"Validation of Trypanosoma brucei trypanothione synthetase as drug target","volume":"36","author":"Comini","year":"2004","journal-title":"Free Radical Biology and Medicine"},{"key":"10.1016\/j.exppara.2008.10.018_bib6","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1111\/j.1550-7408.1977.tb00987.x","article-title":"New culture medium for maintenance of tsetse tissues and growth of trypanosomatids","volume":"24","author":"Cunningham","year":"1977","journal-title":"Journal of Protozoology"},{"key":"10.1016\/j.exppara.2008.10.018_bib7","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.exppara.2006.04.011","article-title":"Leishmania amazonensis: characterization of an ecto-phosphatase activity","volume":"114","author":"De Almeida-Amaral","year":"2006","journal-title":"Experimental Parasitology"},{"key":"10.1016\/j.exppara.2008.10.018_bib8","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.bbagen.2004.08.006","article-title":"Reevaluating the role of 1,10-phenanthroline in oxidative reactions involving ferrous ions and DNA damage","volume":"1675","author":"De Avellar","year":"2004","journal-title":"Biochimica et Biophysica Acta"},{"key":"10.1016\/j.exppara.2008.10.018_bib9","doi-asserted-by":"crossref","first-page":"9486","DOI":"10.1074\/jbc.273.16.9486","article-title":"Characterization of a nucleoside\/proton symporter in procyclic Trypanosoma brucei brucei","volume":"273","author":"De Koning","year":"1998","journal-title":"Journal of Biological Chemistry"},{"key":"10.1016\/j.exppara.2008.10.018_bib10","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.actatropica.2006.05.014","article-title":"Characterization of ecto-phosphatase activities of Trypanosoma cruzi: a comparative study between Colombiana and Y strains","volume":"100","author":"Dutra","year":"2006","journal-title":"Acta Tropica"},{"key":"10.1016\/j.exppara.2008.10.018_bib11","doi-asserted-by":"crossref","first-page":"20221","DOI":"10.1074\/jbc.M610737200","article-title":"Characterization of heme as activator of Toll-like receptor 4","volume":"282","author":"Figueiredo","year":"2007","journal-title":"Journal of Biological Chemistry"},{"key":"10.1016\/j.exppara.2008.10.018_bib12","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.exppara.2005.09.005","article-title":"Trypanosoma rangeli: characterization of a Mg-dependent ecto ATP-diphosphohydrolase activity","volume":"112","author":"Fonseca","year":"2006","journal-title":"Experimental Parasitology"},{"key":"10.1016\/j.exppara.2008.10.018_bib13","first-page":"161","article-title":"Free radical-induced inhibition of ATP diphosphohydrolase activity (EC 3.6.1.5) from rat blood platelets","volume":"41","author":"Frassetto","year":"1997","journal-title":"Biochemistry and Molecular Biology International"},{"key":"10.1016\/j.exppara.2008.10.018_bib14","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/0166-6851(88)90118-1","article-title":"Heme binding to Leishmania mexicana amazonensis","volume":"29","author":"Galbraith","year":"1988","journal-title":"Molecular and Biochemical Parasitology"},{"key":"10.1016\/j.exppara.2008.10.018_bib15","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1042\/bj0900147","article-title":"A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity","volume":"90","author":"Glynn","year":"1964","journal-title":"Biochemical Journal"},{"key":"10.1016\/j.exppara.2008.10.018_bib16","doi-asserted-by":"crossref","first-page":"9673","DOI":"10.1074\/jbc.274.14.9673","article-title":"Urate synthesis in the blood-sucking insect Rhodnius prolixus: stimulation by hemin is mediated by protein kinase C","volume":"274","author":"Gra\u00e7a-Souza","year":"1999","journal-title":"Journal of Biological Chemistry"},{"key":"10.1016\/j.exppara.2008.10.018_bib17","doi-asserted-by":"crossref","first-page":"4160","DOI":"10.1182\/blood.V99.11.4160","article-title":"Neutrophil activation by heme: implications for inflammatory processes","volume":"99","author":"Gra\u00e7a-Souza","year":"2002","journal-title":"Blood"},{"key":"10.1016\/j.exppara.2008.10.018_bib18","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.ibmb.2006.01.009","article-title":"Adaptations against heme toxicity in blood-feeding arthropods","volume":"36","author":"Gra\u00e7a-Souza","year":"2006","journal-title":"Insect Biochemistry and Molecular Biology"},{"key":"10.1016\/j.exppara.2008.10.018_bib19","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/0014-5793(86)80626-3","article-title":"Iron promoters of the Fenton reaction and lipid peroxidation can be released from haemoglobin by peroxides","volume":"201","author":"Gutteridge","year":"1986","journal-title":"FEBS Letters"},{"key":"10.1016\/j.exppara.2008.10.018_bib20","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1042\/bj2560861","article-title":"Antioxidant protection by haemopexin of haem-stimulated lipid peroxidation","volume":"256","author":"Gutteridge","year":"1988","journal-title":"Biochemical Journal"},{"key":"10.1016\/j.exppara.2008.10.018_bib21","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/0166-6851(84)90117-8","article-title":"Purine and pyrimidine metabolism in the Trypanosomatidae","volume":"13","author":"Hammond","year":"1984","journal-title":"Molecular and Biochemical Parasitology"},{"key":"10.1016\/j.exppara.2008.10.018_bib22","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1074\/jbc.272.2.1076","article-title":"Complementary DNA cloning and sequencing of the chicken muscle ecto-ATPase. Homology with the lymphoid cell activation antigen CD39","volume":"272","author":"Kirley","year":"1997","journal-title":"Journal of Biological Chemistry"},{"key":"10.1016\/j.exppara.2008.10.018_bib23","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.bbrc.2006.12.238","article-title":"Heme requirement and intracellular trafficking in Trypanosoma cruzi epimastigotes","volume":"355","author":"Lara","year":"2007","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"10.1016\/j.exppara.2008.10.018_bib24","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/0005-2736(88)90456-7","article-title":"Inhibition of membrane erythrocyte (Ca2++Mg2+)-ATPase by hemin","volume":"946","author":"Leclerc","year":"1988","journal-title":"Biochimica et Biophysica Acta"},{"key":"10.1016\/j.exppara.2008.10.018_bib25","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.exppara.2006.09.002","article-title":"Trypanosoma brucei brucei: biochemical characterization of ecto-nucleoside triphosphate diphosphohydrolase activities","volume":"115","author":"Leite","year":"2007","journal-title":"Experimental Parasitology"},{"key":"10.1016\/j.exppara.2008.10.018_bib26","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/S0021-9258(17)41386-X","article-title":"The determination of inorganic phosphate in the presence of labile phosphate esters","volume":"162","author":"Lowry","year":"1946","journal-title":"Journal of Biological Chemistry"},{"key":"10.1016\/j.exppara.2008.10.018_bib31","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1034\/j.1600-079X.2003.00041.x","article-title":"Melatonin, xanthurenic acid, resveratrol, EGCG, vitamin C and alpha-lipoic acid differentially reduce oxidative DNA damage induced by Fenton reagents: a study of their individual and synergistic actions","volume":"34","author":"L\u00f3pez-Burillo","year":"2003","journal-title":"Journal of Pineal Research"},{"key":"10.1016\/j.exppara.2008.10.018_bib27","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.1016\/0006-2952(77)90167-8","article-title":"Heme lysis of the bloodstream forms of Trypanosoma brucei","volume":"26","author":"Meshinick","year":"1977","journal-title":"Biochemical Pharmacology"},{"key":"10.1016\/j.exppara.2008.10.018_bib28","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/S1383-5769(02)00017-X","article-title":"Ecto-ATPases in protozoa parasites: looking for a function","volume":"51","author":"Meyer-Fernandes","year":"2002","journal-title":"Parasitology International"},{"key":"10.1016\/j.exppara.2008.10.018_bib29","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1016\/S0891-5849(98)00084-7","article-title":"Oxidative damage to sarcoplasmic reticulum Ca2+-ATPase at submicromolar iron concentrations: evidence for metal-catalyzed oxidation","volume":"25","author":"Moreau","year":"1998","journal-title":"Free Radical Biology and Medicine"},{"key":"10.1016\/j.exppara.2008.10.018_bib30","doi-asserted-by":"crossref","first-page":"306","DOI":"10.3177\/jnsv.47.306","article-title":"Xanthurenic acid inhibits metal ion-induced lipid peroxidation and protects NADP-isocitrate dehydrogenase from oxidative inactivation","volume":"47","author":"Murakami","year":"2001","journal-title":"Journal of Nutritional Science and Vitaminology (Tokyo)"},{"key":"10.1016\/j.exppara.2008.10.018_bib32","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1006\/bbrc.2000.3025","article-title":"Heme degradation during the autoxidation of oxyhemoglobin","volume":"273","author":"Nagababu","year":"2000","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"10.1016\/j.exppara.2008.10.018_bib33","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0003-2697(79)90738-3","article-title":"Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction","volume":"95","author":"Ohkawa","year":"1979","journal-title":"Analytical Biochemistry"},{"key":"10.1016\/j.exppara.2008.10.018_bib34","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.exppara.2008.01.003","article-title":"Leishmania amazonensis: effects of heat shock on ecto-ATPase activity","volume":"119","author":"Peres-Sampaio","year":"2008","journal-title":"Experimental Parasitology"},{"key":"10.1016\/j.exppara.2008.10.018_bib35","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/j.exppara.2006.09.019","article-title":"Entamoeba histolytica: an ecto-phosphatase activity regulated by oxidation\u2013reduction reactions","volume":"115","author":"Pinheiro","year":"2007","journal-title":"Experimental Parasitology"},{"key":"10.1016\/j.exppara.2008.10.018_bib36","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.exppara.2008.02.006","article-title":"Giardia lamblia: biochemical characterization of an ecto-ATPase activity","volume":"119","author":"Pinheiro","year":"2008","journal-title":"Experimental Parasitology"},{"key":"10.1016\/j.exppara.2008.10.018_bib37","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.exppara.2006.02.007","article-title":"Leishmania amazonensis: biological and biochemical characterization of ecto-nucleoside triphosphate diphosphohydrolase activities","volume":"114","author":"Pinheiro","year":"2006","journal-title":"Experimental Parasitology"},{"key":"10.1016\/j.exppara.2008.10.018_bib38","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1515\/znc-2000-7-819","article-title":"Carbohydrates protect mitochondrial FoF1ATPase complex against thermal inactivation","volume":"55","author":"Saad-Nehme","year":"2000","journal-title":"Zeitschrift Fur Naturforschung C"},{"key":"10.1016\/j.exppara.2008.10.018_bib39","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/0305-0491(82)90523-5","article-title":"Porphyrin biosynthesis in parasitic hemoflagellates: functional and defective enzymes in Trypanosoma cruzi","volume":"72","author":"Salzman","year":"1982","journal-title":"Comparative Biochemistry and Physiology B"},{"key":"10.1016\/j.exppara.2008.10.018_bib40","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1006\/abbi.1993.1566","article-title":"Hemin-induced lipid membrane disorder and increased permeability: a molecular model for the mechanism of cell lysis","volume":"307","author":"Schmitt","year":"1993","journal-title":"Archives of Biochemistry and Biophysics"},{"key":"10.1016\/j.exppara.2008.10.018_bib41","doi-asserted-by":"crossref","first-page":"25120","DOI":"10.1074\/jbc.M301975200","article-title":"Antimonial-induced increase in intracellular Ca2+ through non-selective cation channels in the host and the parasite is responsible for apoptosis of intracellular Leishmania donovani amastigotes","volume":"278","author":"Sudhandiran","year":"2003","journal-title":"Journal of Biological Chemistry"},{"key":"10.1016\/j.exppara.2008.10.018_bib42","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1093\/oxfordjournals.bmb.a072036","article-title":"Developmental cycles and biology of pathogenic trypanosomes","volume":"41","author":"Vickerman","year":"1985","journal-title":"British Medical Bulletin"},{"key":"10.1016\/j.exppara.2008.10.018_bib43","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1002\/ddr.1097","article-title":"Ectonucleotidases: some recent developments and a note on nomenclature","volume":"52","author":"Zimmermann","year":"2001","journal-title":"Drug Development Research"}],"container-title":["Experimental Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489408002774?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489408002774?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T06:20:38Z","timestamp":1758003638000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014489408002774"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,2]]},"references-count":44,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2009,2]]}},"alternative-id":["S0014489408002774"],"URL":"https:\/\/doi.org\/10.1016\/j.exppara.2008.10.018","relation":{},"ISSN":["0014-4894"],"issn-type":[{"type":"print","value":"0014-4894"}],"subject":[],"published":{"date-parts":[[2009,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Trypanosoma brucei brucei: Effects of ferrous iron and heme on ecto-nucleoside triphosphate diphosphohydrolase activity","name":"articletitle","label":"Article Title"},{"value":"Experimental Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exppara.2008.10.018","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2008 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}