{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T21:04:57Z","timestamp":1780088697643,"version":"3.54.0"},"reference-count":28,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004429","name":"University of Crete","doi-asserted-by":"publisher","award":["2561\/2007"],"award-info":[{"award-number":["2561\/2007"]}],"id":[{"id":"10.13039\/501100004429","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Parasitology"],"published-print":{"date-parts":[[2010,8]]},"DOI":"10.1016\/j.exppara.2010.03.006","type":"journal-article","created":{"date-parts":[[2010,3,23]],"date-time":"2010-03-23T06:13:54Z","timestamp":1269324834000},"page":"384-388","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"title":["Leishmania donovani s.l.: Evaluation of the proliferation potential of promastigotes using CFSE staining and flow cytometry"],"prefix":"10.1016","volume":"125","author":[{"given":"I.","family":"Messaritakis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Mazeris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Koutala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Antoniou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.exppara.2010.03.006_bib1","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1126\/science.1169464","article-title":"Demonstration of genetic exchange during cyclical development of Leishmania in the sand fly vector","volume":"324","author":"Akopyants","year":"2009","journal-title":"Science"},{"key":"10.1016\/j.exppara.2010.03.006_bib2","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/S0966-842X(98)01324-9","article-title":"The biogenesis and properties of the parasitophorous vacuoles that harbour Leishmania in murine macrophages","volume":"6","author":"Antoine","year":"1998","journal-title":"Trends in Microbiology"},{"key":"10.1016\/j.exppara.2010.03.006_bib3","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/S1473-3099(07)70297-9","article-title":"Leishmania donovani leishmaniasis in Cyprus","volume":"8","author":"Antoniou","year":"2008","journal-title":"Lancet Infectious Diseases"},{"key":"10.1016\/j.exppara.2010.03.006_bib4","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/S1473-3099(09)70004-0","article-title":"Leishmania donovani leishmaniasis in Cyprus","volume":"9","author":"Antoniou","year":"2009","journal-title":"Lancet Infectious Diseases"},{"key":"10.1016\/j.exppara.2010.03.006_bib5","doi-asserted-by":"crossref","first-page":"601","DOI":"10.2174\/1566524043360285","article-title":"New insights into the developmental biology and transmission mechanisms of Leishmania","volume":"4","author":"Bates","year":"2004","journal-title":"Current Molecular Medicine"},{"key":"10.1016\/j.exppara.2010.03.006_bib6","doi-asserted-by":"crossref","first-page":"3102","DOI":"10.4049\/jimmunol.155.6.3102","article-title":"Role of the Leishmania surface protease gp63 in complement fixation, cell adhesion, and resistance to complement-mediated lysis","volume":"155","author":"Brittingham","year":"1995","journal-title":"Journal of Immunology"},{"key":"10.1016\/j.exppara.2010.03.006_bib7","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1016\/S0020-7519(01)00259-4","article-title":"Life in vacuoles \u2013 nutrient acquisition by Leishmania amastigotes","volume":"31","author":"Burchmore","year":"2001","journal-title":"International Journal for Parasitology"},{"key":"10.1016\/j.exppara.2010.03.006_bib8","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1016\/j.tim.2004.10.006","article-title":"Rocking the curve","volume":"12","author":"Choisy","year":"2004","journal-title":"Trends in Microbiology"},{"key":"10.1016\/j.exppara.2010.03.006_bib9","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/S0925-4439(99)00065-4","article-title":"Glycoconjugates in Leishmania infectivity","volume":"1455","author":"Descoteaux","year":"1999","journal-title":"Biochimica et Biophysica Acta"},{"key":"10.1016\/j.exppara.2010.03.006_bib10","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1016\/S0020-7519(03)00142-5","article-title":"Two separate growth phases during the development of Leishmania in sand flies: implications for understanding the life cycle","volume":"33","author":"Gossage","year":"2003","journal-title":"International Journal for Parasitology"},{"key":"10.1016\/j.exppara.2010.03.006_bib11","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1080\/00034983.1992.11812717","article-title":"Decreased sensitivity to meglumine antimoniate (Glucantime) of Leishmania infantum isolated from dogs after several courses of drug treatment","volume":"86","author":"Gramiccia","year":"1992","journal-title":"Annals of Tropical Medicine and Parasitology"},{"key":"10.1016\/j.exppara.2010.03.006_bib12","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1002\/1097-0320(20000801)40:4<353::AID-CYTO11>3.0.CO;2-0","article-title":"Flow cytometric assessment of allopurinol susceptibility in Leishmania infantum promastigote","volume":"40","author":"Kamau","year":"2000","journal-title":"Cytometry"},{"key":"10.1016\/j.exppara.2010.03.006_bib13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2210-1-1","article-title":"Flow cytometry analysis of the effect of allopurinol and the dinitroaniline compound (Chloralin) on the viability and proliferation of Leishmania infantum promastigotes","volume":"1","author":"Kamau","year":"2001","journal-title":"BMC Pharmacology"},{"key":"10.1016\/j.exppara.2010.03.006_bib14","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.1016\/S1286-4579(00)01331-9","article-title":"The biological and immunomodulatory properties of sand fly saliva and its role in the establishment of Leishmania infections","volume":"2","author":"Kamhawi","year":"2000","journal-title":"Microbes and Infection"},{"key":"10.1016\/j.exppara.2010.03.006_bib1a","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1016\/j.ijpara.2007.04.007","article-title":"The amastigote forms of Leishmania are experts at exploiting host cell processes to establish infection and persist","volume":"37","author":"Kima","year":"2007","journal-title":"International Journal for Parasitology"},{"key":"10.1016\/j.exppara.2010.03.006_bib15","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/S1473-3099(08)70132-4","article-title":"Leishmania donovani leishmaniasis in Cyprus","volume":"8","author":"Leger","year":"2008","journal-title":"Lancet Infectious Diseases"},{"key":"10.1016\/j.exppara.2010.03.006_bib16","first-page":"99","article-title":"Requirements of defined cultivation conditions for standard growth of Leishmania promastigotes in vitro","volume":"45","author":"Lemesre","year":"1988","journal-title":"Acta Tropica"},{"key":"10.1016\/j.exppara.2010.03.006_bib17","doi-asserted-by":"crossref","first-page":"296","DOI":"10.4269\/ajtmh.1983.32.296","article-title":"Comparison of microscopy and culture in the detection of Leishmania donovani from splenic aspirates","volume":"32","author":"Lightner","year":"1983","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"10.1016\/j.exppara.2010.03.006_bib18","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1590\/S0074-02762008000400018","article-title":"Isolation and molecular identification of Leishmania chagasi from a bat (Carollia perspicillata) in northeastern Venezuela","volume":"103","author":"Lima","year":"2008","journal-title":"Mem\u00f3rias do Instituto Oswaldo Cruz"},{"key":"10.1016\/j.exppara.2010.03.006_bib19","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0022-1759(00)00231-3","article-title":"Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution","volume":"243","author":"Lyons","year":"2000","journal-title":"Journal of Immunological Methods"},{"key":"10.1016\/j.exppara.2010.03.006_bib1b","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1016\/S0020-7519(07)00216-0","article-title":"The molecular basis of Leishmania pathogenesis","volume":"37","author":"McConville","year":"2007","journal-title":"International Journal for Parasitology"},{"key":"10.1016\/j.exppara.2010.03.006_bib20","first-page":"437","article-title":"Cultivation of Leishmania sp. in nutrient broth","volume":"25","author":"Ozbilgin","year":"1995","journal-title":"Journal of Egyptian Society of Parasitology"},{"key":"10.1016\/j.exppara.2010.03.006_bib21","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1016\/j.ijpara.2006.06.019","article-title":"First report of genetic hybrids between two very divergent Leishmania species: Leishmania infantum and Leishmania major","volume":"36","author":"Ravel","year":"2006","journal-title":"International Journal of Parasitology"},{"key":"10.1016\/j.exppara.2010.03.006_bib22","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/S0169-4758(00)01692-6","article-title":"Leishmania\u2013host\u2013cell interaction: complexities and alternative views","volume":"16","author":"Rittig","year":"2000","journal-title":"Parasitology Today"},{"key":"10.1016\/j.exppara.2010.03.006_bib23","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1017\/S0031182002001439","article-title":"The role of promastigote secretory gel in the origin and transmission of the infective stage of Leishmania mexicana by the sandfly Lutzomyia longipalpis","volume":"124","author":"Rogers","year":"2002","journal-title":"Parasitology"},{"key":"10.1016\/j.exppara.2010.03.006_bib24","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1126\/science.6701528","article-title":"Identification of an infective stage of Leishmania promastigotes","volume":"223","author":"Sacks","year":"1984","journal-title":"Science"},{"key":"10.1016\/j.exppara.2010.03.006_bib25","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1111\/j.1365-2958.2006.05205.x","article-title":"Leishmania histone H1 overexpression delays parasite cell-cycle progression, parasite differentiation and reduces Leishmania infectivity in vivo","volume":"60","author":"Smirlis","year":"2006","journal-title":"Molecular Microbiology"},{"key":"10.1016\/j.exppara.2010.03.006_bib26","unstructured":"WHO, 1981. Geographical Distribution of Arthropod-borne Diseases and their Principal Vectors. Report of the Third Meeting of the Scientific Working Group on Leishmaniases. World Health Organization, Geneva."}],"container-title":["Experimental Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489410000913?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489410000913?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T06:21:03Z","timestamp":1758003663000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014489410000913"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,8]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2010,8]]}},"alternative-id":["S0014489410000913"],"URL":"https:\/\/doi.org\/10.1016\/j.exppara.2010.03.006","relation":{},"ISSN":["0014-4894"],"issn-type":[{"value":"0014-4894","type":"print"}],"subject":[],"published":{"date-parts":[[2010,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Leishmania donovani s.l.: Evaluation of the proliferation potential of promastigotes using CFSE staining and flow cytometry","name":"articletitle","label":"Article Title"},{"value":"Experimental Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exppara.2010.03.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2010 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}