{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T21:31:40Z","timestamp":1767994300150,"version":"3.49.0"},"reference-count":32,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Parasitology"],"published-print":{"date-parts":[[2011,1]]},"DOI":"10.1016\/j.exppara.2010.06.017","type":"journal-article","created":{"date-parts":[[2010,6,20]],"date-time":"2010-06-20T20:25:03Z","timestamp":1277065503000},"page":"18-24","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":22,"title":["Trypanosoma brucei: Immunisation with plasmid DNA encoding invariant surface glycoprotein gene is able to induce partial protection in experimental African trypanosomiasis"],"prefix":"10.1016","volume":"127","author":[{"given":"Andreia Sofia Cruz","family":"Lan\u00e7a","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karina Pires","family":"de Sousa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Atouguia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duarte Miguel F.","family":"Prazeres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel Amaro","family":"Monteiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo Sousa","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.exppara.2010.06.017_b0005","doi-asserted-by":"crossref","first-page":"6017","DOI":"10.1128\/IAI.73.9.6017-6025.2005","article-title":"CD8+-T-cell-dependent control of Trypanosoma cruzi infection in a highly susceptible mouse strain after immunization with recombinant proteins based on amastigote surface protein 2","volume":"73","author":"Ara\u00fajo","year":"2005","journal-title":"Infec. Immun."},{"key":"10.1016\/j.exppara.2010.06.017_b0010","series-title":"Infectious Diseases of the Nervous System","first-page":"321","article-title":"Neurological aspects of human African trypanosomiasis","author":"Atouguia","year":"2000"},{"key":"10.1016\/j.exppara.2010.06.017_b0015","first-page":"309422","article-title":"The genome of the African trypanosome Trypanosoma brucei","volume":"309416","author":"Berriman","year":"2005","journal-title":"Science"},{"key":"10.1016\/j.exppara.2010.06.017_b0020","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1038\/362603a0","article-title":"A structural motif in the variant surface glycoproteins of Trypanosoma brucei","volume":"362","author":"Blum","year":"1993","journal-title":"Nature"},{"key":"10.1016\/j.exppara.2010.06.017_b0025","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1586\/erv.09.158","article-title":"DNA vaccines: a rational design against parasitic diseases","volume":"9","author":"Carvalho","year":"2010","journal-title":"Expert. Rev. Vaccines"},{"key":"10.1016\/j.exppara.2010.06.017_b0030","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1146\/annurev.iy.08.040190.000503","article-title":"Cellular and genetic aspects on antigenic variation in trypanosomes","volume":"8","author":"Cross","year":"1990","journal-title":"Annu. Rev. Immunol."},{"key":"10.1016\/j.exppara.2010.06.017_b0035","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1002\/(SICI)1097-0290(20000605)68:5<576::AID-BIT13>3.0.CO;2-5","article-title":"Purification of a cystic fibrosis plasmid vector for gene therapy using hydrophobic interaction chromatography","volume":"68","author":"Diogo","year":"2000","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.exppara.2010.06.017_b0040","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.mib.2003.09.007","article-title":"Treatment of infectious diseases with immunostimulatory oligodeoxynucleotides containing CpG motifs","volume":"6","author":"Dittmer","year":"2003","journal-title":"Curr. Opin. Microbiol."},{"key":"10.1016\/j.exppara.2010.06.017_b0045","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1128\/IAI.72.1.46-53.2004","article-title":"Immunotherapy of Trypanosoma cruzi infection with DNA vaccines in mice","volume":"72","author":"Dumonteil","year":"2004","journal-title":"Infect. Immun."},{"key":"10.1016\/j.exppara.2010.06.017_b0050","series-title":"Principles and Practice of Clinical Parasitology","author":"Gillespie","year":"2001"},{"key":"10.1016\/j.exppara.2010.06.017_b0055","doi-asserted-by":"crossref","first-page":"3011","DOI":"10.1016\/S0264-410X(00)00109-2","article-title":"Therapy of murine cutaneous leishmaniasis by DNA vaccination","volume":"18","author":"Handman","year":"2000","journal-title":"Vaccine"},{"key":"10.1016\/j.exppara.2010.06.017_b0060","doi-asserted-by":"crossref","first-page":"2366","DOI":"10.1128\/IAI.01649-06","article-title":"CpG oligodeoxynucleotide treatment enhances innate resistance and acquired immunity to African trypanosomes","volume":"75","author":"Harris","year":"2007","journal-title":"Infect. Immun."},{"key":"10.1016\/j.exppara.2010.06.017_b0065","doi-asserted-by":"crossref","first-page":"6775","DOI":"10.4049\/jimmunol.161.12.6775","article-title":"Resistance to the African trypanosomes is IFN-gamma dependent","volume":"161","author":"Hertz","year":"1998","journal-title":"J. Immunol."},{"key":"10.1016\/j.exppara.2010.06.017_b0070","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/0001-706X(93)90093-Q","article-title":"Combination chemotherapy of CNS trypanosomiasis","volume":"54","author":"Jennings","year":"1993","journal-title":"Acta Trop."},{"key":"10.1016\/j.exppara.2010.06.017_b0075","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/S0165-5728(99)00196-4","article-title":"The pathogenesis and modulation of post-treatment reactive encephalopathy in a mouse model of human African trypanosomiasis","volume":"100","author":"Kennedy","year":"2000","journal-title":"J. Neuroimmunol."},{"key":"10.1016\/j.exppara.2010.06.017_b0080","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1038\/nri1329","article-title":"Immunotherapy uses of CpG oligodeoxynucleotides","volume":"4","author":"Klinman","year":"2004","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.exppara.2010.06.017_b0085","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1080\/08830180600785827","article-title":"From plasmids to protection: a review of DNA vaccines against infectious diseases","volume":"25","author":"Laddy","year":"2006","journal-title":"Intern. Rev. Immunol."},{"key":"10.1016\/j.exppara.2010.06.017_b0090","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/0014-4894(70)90120-7","article-title":"Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose","volume":"28","author":"Lanham","year":"1970","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.exppara.2010.06.017_b0095","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1128\/iai.34.2.513-518.1981","article-title":"Genetics of resistance to African trypanosomes: role of the H-2 locus in determining resistance to infection with Trypanosoma rhodesiense","volume":"34","author":"Levine","year":"1981","journal-title":"Infect. Immun."},{"key":"10.1016\/j.exppara.2010.06.017_b0100","doi-asserted-by":"crossref","first-page":"1564","DOI":"10.4049\/jimmunol.133.3.1564","article-title":"Genetics of resistance to the African trypanosomes. III. Variant-specific antibody responses of H-2-compatible resistant and susceptible mice","volume":"133","author":"Levine","year":"1984","journal-title":"J. Immunol."},{"key":"10.1016\/j.exppara.2010.06.017_b0105","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1038\/22326","article-title":"Therapy of tuberculosis in mice by DNA vaccination","volume":"400","author":"Lowrie","year":"1999","journal-title":"Nature"},{"key":"10.1016\/j.exppara.2010.06.017_b0110","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1111\/j.1365-3024.2005.00791.x","article-title":"Regulation of innate and acquired immunity in African trypanosomiasis","volume":"27","author":"Mansfield","year":"2005","journal-title":"Parasite Immunol."},{"key":"10.1016\/j.exppara.2010.06.017_b0115","doi-asserted-by":"crossref","first-page":"2910","DOI":"10.4049\/jimmunol.150.7.2910","article-title":"Characterization of T helper cell responses to the trypanosome variant surface glycoprotein","volume":"150","author":"Schleifer","year":"1993","journal-title":"J. Immunol."},{"key":"10.1016\/j.exppara.2010.06.017_b0120","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1128\/IAI.66.2.451-461.1998","article-title":"Interleukin-4-dependent immunoglobulin G1 isotype switch in the presence of a polarized antigen-specific Th1-cell response to the trypanosome variant surface glycoprotein","volume":"66","author":"Schopf","year":"1998","journal-title":"Infect. Immun."},{"key":"10.1016\/j.exppara.2010.06.017_b0125","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1371\/journal.pmed.0050055","article-title":"Eliminating human African trypanosomiasis: where do we stand and what comes next?","volume":"5","author":"Simarro","year":"2008","journal-title":"PLoS Med."},{"key":"10.1016\/j.exppara.2010.06.017_b0130","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1038\/35021239","article-title":"Vaccine against intracellular infections requiring cellular immunity","volume":"406","author":"Seder","year":"2000","journal-title":"Nature"},{"key":"10.1016\/j.exppara.2010.06.017_b0135","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1007\/s00436-009-1542-6","article-title":"Trans-sialidase from Trypanosoma brucei as a potential target for DNA vaccine development against African trypanosomiasis","volume":"105","author":"Silva","year":"2009","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.exppara.2010.06.017_b0140","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1590\/S0001-37652006000400004","article-title":"Immunology and immunopathology of African trypanosomiasis","volume":"78","author":"Vicendeau","year":"2006","journal-title":"Ann. Braz. Acad. Sci."},{"key":"10.1016\/j.exppara.2010.06.017_b0145","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/0166-6851(94)00194-R","article-title":"Genomic organization of an invariant surface glycoprotein gene family of Trypanosoma brucei","volume":"69","author":"Ziegelbauer","year":"1995","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.exppara.2010.06.017_b0150","doi-asserted-by":"crossref","first-page":"10791","DOI":"10.1016\/S0021-9258(19)50088-6","article-title":"Identification of invariant surface glycoprotein in the bloodstream stage of Trypanosoma brucei","volume":"267","author":"Ziegelbauer","year":"1992","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.exppara.2010.06.017_b0155","doi-asserted-by":"crossref","first-page":"10797","DOI":"10.1016\/S0021-9258(19)50089-8","article-title":"Molecular characterization of two invariant surface glycoproteins specific for the bloodstream stage of Trypanoma brucei","volume":"267","author":"Ziegelbauer","year":"1992","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.exppara.2010.06.017_b0160","doi-asserted-by":"crossref","first-page":"10817","DOI":"10.1073\/pnas.181123498","article-title":"Induction of CD4+ T cell-dependent CD8+ type 1 responses in humans by a malaria DNA vaccine","volume":"11","author":"Wang","year":"2001","journal-title":"PNAS"}],"container-title":["Experimental Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489410002122?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489410002122?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T06:01:41Z","timestamp":1762149701000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014489410002122"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,1]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,1]]}},"alternative-id":["S0014489410002122"],"URL":"https:\/\/doi.org\/10.1016\/j.exppara.2010.06.017","relation":{},"ISSN":["0014-4894"],"issn-type":[{"value":"0014-4894","type":"print"}],"subject":[],"published":{"date-parts":[[2011,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Trypanosoma brucei: Immunisation with plasmid DNA encoding invariant surface glycoprotein gene is able to induce partial protection in experimental African trypanosomiasis","name":"articletitle","label":"Article Title"},{"value":"Experimental Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exppara.2010.06.017","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2010 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}