{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T11:33:43Z","timestamp":1790249623792,"version":"4.1.0"},"reference-count":36,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2013,6,1]],"date-time":"2013-06-01T00:00:00Z","timestamp":1370044800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["30972579"],"award-info":[{"award-number":["30972579"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["30972492"],"award-info":[{"award-number":["30972492"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81141084"],"award-info":[{"award-number":["81141084"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81271860"],"award-info":[{"award-number":["81271860"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Parasitology"],"published-print":{"date-parts":[[2013,6]]},"DOI":"10.1016\/j.exppara.2013.03.004","type":"journal-article","created":{"date-parts":[[2013,3,15]],"date-time":"2013-03-15T14:35:12Z","timestamp":1363358112000},"page":"148-154","source":"Crossref","is-referenced-by-count":81,"title":["Cloning, expression and characterization of a Trichinella spiralis serine protease gene encoding a 35.5kDa protein"],"prefix":"10.1016","volume":"134","author":[{"given":"Bin","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong Quan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui Jun","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li Na","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.exppara.2013.03.004_b0005","first-page":"487","article-title":"Rapid expulsion of Trichinella spiralis in suckling rats: mediation by monoclonal antibodies","volume":"65","author":"Appleton","year":"1988","journal-title":"Immunology"},{"key":"10.1016\/j.exppara.2013.03.004_b0010","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/S0166-6851(97)00145-X","article-title":"Identification of serine\/threonine protein kinases secreted by Trichinella spiralis infective larvae","volume":"90","author":"Arden","year":"1997","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.exppara.2013.03.004_b0015","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/1477-5956-10-10","article-title":"Comparative analysis of excretory-secretory antigens of Trichinella spiralis and Trichinella britovi muscle larvae by two-dimensional difference gel electrophoresis and immunoblotting","volume":"10","author":"Bien","year":"2012","journal-title":"Proteome Sci."},{"key":"10.1016\/j.exppara.2013.03.004_b0020","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.rvsc.2006.01.002","article-title":"TSL-1 antigens of Trichinella: an overview of their potential role in parasite invasion, survival and serodiagnosis of trichinellosis","volume":"81","author":"Bol\u00e1s-Fernandez","year":"2006","journal-title":"Res. Vet. Sci."},{"key":"10.1016\/j.exppara.2013.03.004_b0025","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/0304-4017(92)90035-8","article-title":"Proteolytic enzymes from Trichinella spiralis larvae","volume":"45","author":"Criado-Fornelio","year":"1992","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.exppara.2013.03.004_b0030","first-page":"235","article-title":"Seroepidemiologicl study of Trichinella spiralis infection in central China","volume":"36","author":"Cui","year":"1999","journal-title":"Helminthologia"},{"key":"10.1016\/j.exppara.2013.03.004_b0035","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.vetpar.2008.10.036","article-title":"Cloning and analysis of a Trichinella pseudospiralis muscle larva secreted serine protease gene","volume":"59","author":"Cwiklinski","year":"2009","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.exppara.2013.03.004_b0040","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/0166-6851(90)90187-Q","article-title":"Characterization of Trichinella spiralis antigens sharing an immunodominant, carbohydrate-associated determinant distinct from phosphorylcholine","volume":"41","author":"Denkers","year":"1990","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.exppara.2013.03.004_b0045","doi-asserted-by":"crossref","first-page":"933","DOI":"10.2307\/3278441","article-title":"A circular thermal migration device for the rapid collection of large numbers of intestinal helminths","volume":"59","author":"Despommier","year":"1973","journal-title":"J. Parasitol."},{"key":"10.1016\/j.exppara.2013.03.004_b0050","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/S0169-4758(98)01287-3","article-title":"How does Trichinella spiralis make itself at home?","volume":"14","author":"Despommier","year":"1998","journal-title":"Parasitol. Today"},{"key":"10.1016\/j.exppara.2013.03.004_b0055","doi-asserted-by":"crossref","first-page":"33","DOI":"10.18388\/abp.2006_3361","article-title":"Molecules released by helminth parasites involved in host colonization","volume":"53","author":"Dzik","year":"2006","journal-title":"Acta. Biochim. Pol."},{"key":"10.1016\/j.exppara.2013.03.004_b0060","article-title":"Vaccination of mice with an antigenic serine protease-like protein elicits a protective immune response against Trichinella spiralis infection","author":"Feng","year":"2012","journal-title":"J. Parasitol."},{"key":"10.1016\/j.exppara.2013.03.004_b0065","series-title":"Antibodies: A laboratory manual Cold Spring Harbor Laboratory","author":"Harlow","year":"1988"},{"key":"10.1016\/j.exppara.2013.03.004_b0070","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1017\/S003118200300310X","article-title":"Monoclonal IgA antibody mediated expulsion of Trichinella from the intestine of mice","volume":"126","author":"Inaba","year":"2003","journal-title":"Parasitology"},{"key":"10.1016\/j.exppara.2013.03.004_b0075","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1089\/fpd.2011.0985","article-title":"Comparison of artificial digestion and Baermann\u2019s methods for detection of Trichinella spiralis pre-encapsulated larvae in muscles with low-level infections","volume":"9","author":"Jiang","year":"2012","journal-title":"Foodborne Pathog. Dis."},{"key":"10.1016\/j.exppara.2013.03.004_b0080","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1089\/fpd.2009.0445","article-title":"Factors affecting the sensitivity of artificial digestion and its optimization for inspection of Trichinella spiralis in meat","volume":"7","author":"Li","year":"2010","journal-title":"Foodborne Pathog. Dis."},{"key":"10.1016\/j.exppara.2013.03.004_b0085","doi-asserted-by":"crossref","first-page":"4806","DOI":"10.1128\/IAI.65.11.4806-4812.1997","article-title":"Invasion of intestinal epithelia in vitro by the parasitic nematode Trichinella spiralis","volume":"65","author":"ManWarren","year":"1997","journal-title":"Infect. Immun."},{"key":"10.1016\/j.exppara.2013.03.004_b0090","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1042\/bj2400001","article-title":"The purification of eukaryotic polypeptides synthesized in Escherichia coli","volume":"240","author":"Marston","year":"1986","journal-title":"Biochem. J."},{"key":"10.1016\/j.exppara.2013.03.004_b0100","doi-asserted-by":"crossref","first-page":"1941","DOI":"10.1128\/IAI.66.5.1941-1945.1998","article-title":"Participation of parasite surface glycoproteins in antibody-mediated protection of epithelia against Trichinella spiralis","volume":"66","author":"McVay","year":"1998","journal-title":"Infect. Immun."},{"key":"10.1016\/j.exppara.2013.03.004_b0095","doi-asserted-by":"crossref","first-page":"1912","DOI":"10.1128\/IAI.68.4.1912-1918.2000","article-title":"Antibodies to tyvelose exhibit multiple modes of interference with the epithelial niche of Trichinella spiralis","volume":"68","author":"McVay","year":"2000","journal-title":"Infect. Immun."},{"key":"10.1016\/j.exppara.2013.03.004_b0105","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/s004360050506","article-title":"Trichinella spiralis: proteinases in the larvae","volume":"85","author":"Moczon","year":"1999","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.exppara.2013.03.004_b0110","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1007\/s00436-008-1248-1","article-title":"Functional genes and proteins of Trichinella spp","volume":"104","author":"Nagano","year":"2009","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.exppara.2013.03.004_b0115","doi-asserted-by":"crossref","first-page":"4525","DOI":"10.1002\/pmic.200402057","article-title":"Proteomic analysis of the excretory-secretory proteins of the Trichinella spiralis L1 larva, a nematode parasite of skeletal muscle","volume":"5","author":"Robinson","year":"2005","journal-title":"Proteomics"},{"key":"10.1016\/j.exppara.2013.03.004_b0120","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.ijpara.2006.08.007","article-title":"Comparative analysis of the excretory-secretory proteome of the muscle larva of Trichinella pseudospiralis and Trichinella spiralis","volume":"37","author":"Robinson","year":"2007","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.exppara.2013.03.004_b0125","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.molbiopara.2006.09.008","article-title":"Secretion and processing of a novel multi-domain cystatin-like protein by intracellular stages of Trichinella spiralis","volume":"151","author":"Robinson","year":"2007","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.exppara.2013.03.004_b0130","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0166-6851(02)00094-4","article-title":"A putative serine protease among the excretory-secretory glycoproteins of L1 Trichinella spiralis","volume":"122","author":"Romaris","year":"2002","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.exppara.2013.03.004_b0135","first-page":"251","article-title":"Collection of newborn larvae of Trichinella spiralis in vitro","volume":"7","author":"Takada","year":"1988","journal-title":"Jpn. J. Parasitol"},{"key":"10.1016\/j.exppara.2013.03.004_b0140","doi-asserted-by":"crossref","first-page":"141","DOI":"10.14411\/fp.2000.027","article-title":"Proteolytic enzymes secreted by larval stage of the parasitic nematode Trichinella spiralis","volume":"47","author":"Todorova","year":"2000","journal-title":"Folia Parasitol."},{"key":"10.1016\/j.exppara.2013.03.004_b0145","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications","volume":"76","author":"Towbin","year":"1979","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.exppara.2013.03.004_b0160","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1016\/j.vaccine.2005.08.104","article-title":"Vaccination of mice with DNA vaccine induces the immune response and partial protection against T. spiralis infection","volume":"24","author":"Wang","year":"2006","journal-title":"Vaccine"},{"key":"10.1016\/j.exppara.2013.03.004_b0150","first-page":"81","article-title":"Effect of immune sera on the invasion of T. spiralis infective larvae into intestinal epithelial cells and their development in vitro","volume":"28","author":"Wang","year":"2010","journal-title":"Chin. J. Parasitol. Parasit. Dis."},{"key":"10.1016\/j.exppara.2013.03.004_b0170","first-page":"901","article-title":"Observation on invasion of intestinal epithelial cells in vitro by Trichinella spiralis larvae and development","volume":"5","author":"Wang","year":"2010","journal-title":"Chin. Pathog. Biol."},{"key":"10.1016\/j.exppara.2013.03.004_b0155","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1007\/s00436-010-2102-9","article-title":"Protein change of intestinal epithelial cells induced in vitro by Trichinella spiralis infective larvae","volume":"108","author":"Wang","year":"2011","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.exppara.2013.03.004_b0165","doi-asserted-by":"crossref","first-page":"2375","DOI":"10.1016\/j.jprot.2012.02.005","article-title":"Proteomic analysis of Trichinella spiralis proteins in intestinal epithelial cells after culture with their larvae by shotgun LC\u2013MS\/MS approach","volume":"75","author":"Wang","year":"2012","journal-title":"J. Proteomics"},{"key":"10.1016\/j.exppara.2013.03.004_b0175","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/0166-6851(93)90155-Q","article-title":"Characterization of novel fucosyland tyvelosyl-containing glycoconjugates from Trichinella spiralis muscle stage larvae","volume":"61","author":"Wisnewski","year":"1993","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.exppara.2013.03.004_b0180","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/j.exppara.2009.12.010","article-title":"Trichinella spiralis: immune response and protective immunity elicited by recombinant paramyosin formulated with different adjuvants","volume":"124","author":"Yang","year":"2010","journal-title":"Exp. Parasitol."}],"container-title":["Experimental Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S001448941300074X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S001448941300074X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T23:00:53Z","timestamp":1595545253000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S001448941300074X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,6]]},"references-count":36,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,6]]}},"alternative-id":["S001448941300074X"],"URL":"https:\/\/doi.org\/10.1016\/j.exppara.2013.03.004","relation":{},"ISSN":["0014-4894"],"issn-type":[{"value":"0014-4894","type":"print"}],"subject":[],"published":{"date-parts":[[2013,6]]}}}