{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T15:04:00Z","timestamp":1790089440346,"version":"4.0.1"},"reference-count":27,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Parasitology"],"published-print":{"date-parts":[[2013,11]]},"DOI":"10.1016\/j.exppara.2013.09.003","type":"journal-article","created":{"date-parts":[[2013,9,13]],"date-time":"2013-09-13T06:45:50Z","timestamp":1379054750000},"page":"551-557","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":52,"title":["A new set of primers directed to 18S rRNA gene for molecular identification of Cryptosporidium spp. and their performance in the detection and differentiation of oocysts shed by synanthropic rodents"],"prefix":"10.1016","volume":"135","author":[{"given":"Sheila O.S.","family":"Silva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo J.","family":"Richtzenhain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iracema N.","family":"Barros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra M.M. C.","family":"Gomes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aristeu V.","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noemila D.","family":"Kozerski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaqueline B.","family":"de Ara\u00fajo Ceranto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara B.","family":"Keid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo M.","family":"Soares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.exppara.2013.09.003_b0005","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1111\/j.1574-6968.1996.tb07987.x","article-title":"Comparison of assays for Cryptosporidium parvum oocysts viability after chemical disinfection","volume":"135","author":"Black","year":"1996","journal-title":"Microbiology Letters"},{"key":"10.1016\/j.exppara.2013.09.003_b0010","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1645\/GE-1020R.1","article-title":"A rapid method for producing highly purified Cryptosporidium parvum oocyts","volume":"93","author":"O\u2019Brien","year":"2007","journal-title":"Journal of Parasitology"},{"key":"10.1016\/j.exppara.2013.09.003_b0015","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1645\/0022-3395(2001)087[1415:CCNSFD]2.0.CO;2","article-title":"Cryptosporidium canis sp from domestic dogs","volume":"87","author":"Fayer","year":"2001","journal-title":"Journal of Parasitology"},{"key":"10.1016\/j.exppara.2013.09.003_b0020","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.exppara.2008.11.005","article-title":"Cryptosporidium in wild placental mammals","volume":"124","author":"Feng","year":"2008","journal-title":"Experimental Parasitology"},{"key":"10.1016\/j.exppara.2013.09.003_b0025","doi-asserted-by":"crossref","first-page":"7693","DOI":"10.1128\/AEM.00848-07","article-title":"Novel Cryptosporidium genotype in wild Australian mice (Mus domesticus)","volume":"73","author":"Foo","year":"2007","journal-title":"Applied Environmental Microbiology"},{"key":"10.1016\/j.exppara.2013.09.003_b0030","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1590\/S1807-59322006000200006","article-title":"Multilocus genotyping of Cryptosporidium hominis associated with diarrhea outbreak in a day care unit in S\u00e3o Paulo","volume":"61","author":"Gon\u00e7alves","year":"2006","journal-title":"Clinics"},{"key":"10.1016\/j.exppara.2013.09.003_b0035","first-page":"95","article-title":"Biedit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","volume":"41","author":"Hall","year":"1999","journal-title":"Nucleic Acids Symposium Series"},{"key":"10.1016\/j.exppara.2013.09.003_b0040","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/S0166-6851(97)00181-3","article-title":"Ribosomal RNA gene organization in Cryptosporidium parvum","volume":"90","author":"Le Blanq","year":"1997","journal-title":"Molecular and Biochemical Parasitology"},{"key":"10.1016\/j.exppara.2013.09.003_b0045","doi-asserted-by":"crossref","first-page":"2335","DOI":"10.1128\/JCM.40.7.2335-2338.2002","article-title":"Detection and differentiation of Cryptosporidium parasites that are pathogenic for humans by real-time PCR","volume":"40","author":"Limor","year":"2002","journal-title":"Journal of Clinical Microbiology"},{"key":"10.1016\/j.exppara.2013.09.003_b0050","doi-asserted-by":"crossref","first-page":"7692","DOI":"10.1128\/AEM.01386-09","article-title":"Cryptosporidium spp. in wild, laboratory, and pet rodents in China: prevalence and molecular characterization","volume":"75","author":"Lv","year":"2009","journal-title":"Applied and Environmental Microbiology"},{"key":"10.1016\/j.exppara.2013.09.003_b0055","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1590\/S0036-46652009000600006","article-title":"Molecular characterization of Cryptosporidium spp. from HIV infected patients from an urban area of Brazil","volume":"51","author":"Lucca","year":"2009","journal-title":"Journal of the Institute of Tropical Medicine of S\u00e3o Paulo"},{"key":"10.1016\/j.exppara.2013.09.003_b0060","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.2307\/3285678","article-title":"Phylogenetic relationships among isolates of Cryptosporidium: evidence for several new species","volume":"85","author":"Morgan","year":"1999","journal-title":"Journal of Parasitology"},{"key":"10.1016\/j.exppara.2013.09.003_b0065","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1017\/S0031182099005703","article-title":"Molecular and phylogenetic analysis of Cryptosporidium muris from various hosts","volume":"120","author":"Morgan","year":"2000","journal-title":"Parasitology"},{"key":"10.1016\/j.exppara.2013.09.003_b0070","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1128\/JCM.44.2.632-634.2006","article-title":"Multilocus genotyping of Cryptosporidium sp isolates from human immunodeficiency virus-infected individuals in South India","volume":"44","author":"Muthusamy","year":"2006","journal-title":"Journal of Clinical Microbiology"},{"key":"10.1016\/j.exppara.2013.09.003_b0075","doi-asserted-by":"crossref","first-page":"7548","DOI":"10.1128\/AEM.01352-06","article-title":"Identification of novel Cryptosporidium genotypes from avian hosts","volume":"72","author":"Ng","year":"2006","journal-title":"Applied and Environmental Microbiology"},{"key":"10.1016\/j.exppara.2013.09.003_b0080","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.meegid.2013.01.011","article-title":"Molecular characterization of Cryptosporidium spp. from wild rats and mice from rural communities in the Philippines","volume":"16","author":"Ng-Hublin","year":"2013","journal-title":"Infection, Genetics and Evolution"},{"issue":"4","key":"10.1016\/j.exppara.2013.09.003_b0085","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1016\/j.exppara.2012.05.009","article-title":"Multiple Cryptosporidium genotypes detected in wild black rats (Rattus rattus) from northern Australia","volume":"131","author":"Paparini","year":"2012","journal-title":"Experimental Parasitology"},{"key":"10.1016\/j.exppara.2013.09.003_b0090","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1093\/molehr\/gag051","article-title":"Detailed investigation of factors influencing amplification efficiency and allele drop-out in single cell PCR: implications for preimplantation genetic diagnosis","volume":"9","author":"Piyamongkol","year":"2003","journal-title":"Molecular Human Reproduction"},{"key":"10.1016\/j.exppara.2013.09.003_b0095","doi-asserted-by":"crossref","first-page":"4992","DOI":"10.1128\/AEM.71.9.4992-4997.2005","article-title":"Sheep may not be an important zoonotic reservoir for Cryptosporidium and Giardia parasites","volume":"71","author":"Ryan","year":"2005","journal-title":"Applied and Evironmental Microbiology"},{"key":"10.1016\/j.exppara.2013.09.003_b0100","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.vetpar.2010.09.031","article-title":"Genotyping of Cryptosporidium spp. from free-living wild birds from Brazil","volume":"175","author":"Sev\u00e1","year":"2011","journal-title":"Veterinary Parasitology"},{"issue":"388\u2013394","key":"10.1016\/j.exppara.2013.09.003_b0105","first-page":"2002","article-title":"Molecular phylogeny and evolutionary relationships of Cryptosporidium parasites at the actin locus","volume":"88","author":"Sulaiman","year":"2002","journal-title":"Journal of Parasitology"},{"key":"10.1016\/j.exppara.2013.09.003_b0110","doi-asserted-by":"crossref","first-page":"4876","DOI":"10.1093\/nar\/25.24.4876","article-title":"The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools","volume":"25","author":"Thompson","year":"1997","journal-title":"Nucleic Acids Researchs"},{"key":"10.1016\/j.exppara.2013.09.003_b0115","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0732-8893(01)00276-0","article-title":"Rapid Methods to isolate Cryptosporidium from frozen feces for PCR","volume":"41","author":"Ward","year":"2001","journal-title":"Diagnostic Microbiology and Infectious Disease"},{"key":"10.1016\/j.exppara.2013.09.003_b0120","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1111\/j.1751-0813.2003.tb14602.x","article-title":"Cryptosporidium muris infection in bilbies (Macrotis lagotis)","volume":"81","author":"Warren","year":"2003","journal-title":"Journal Veterinary Australia"},{"key":"10.1016\/j.exppara.2013.09.003_b0125","doi-asserted-by":"crossref","first-page":"1578","DOI":"10.1128\/AEM.65.4.1578-1583.1999","article-title":"Phylogenetic analysis of Cryptosporidium parasites based on the small subunit ribosomal RNA gene locus","volume":"65","author":"Xiao","year":"1999","journal-title":"Applied and Environmental Microbiology"},{"key":"10.1016\/j.exppara.2013.09.003_b0130","doi-asserted-by":"crossref","first-page":"5492","DOI":"10.1128\/AEM.66.12.5492-5498.2000","article-title":"Identification of species and sources of Cryptosporidium oocysts in storm waters with a small-subnit rRNA \u2013 based diagnostic and genotyping tool","volume":"66","author":"Xiao","year":"2000","journal-title":"Applied and Environmental Microbiology"},{"key":"10.1016\/j.exppara.2013.09.003_b0135","first-page":"1239","article-title":"Molecular characterization of species and genotypes of Cryptosporidium and Giardia and assessment of zoonotic transmission","volume":"38","author":"Xiao","year":"2008","journal-title":"Journal of Parasitology"}],"container-title":["Experimental Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489413002415?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489413002415?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T23:37:39Z","timestamp":1646523459000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014489413002415"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11]]},"references-count":27,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,11]]}},"alternative-id":["S0014489413002415"],"URL":"https:\/\/doi.org\/10.1016\/j.exppara.2013.09.003","relation":{},"ISSN":["0014-4894"],"issn-type":[{"value":"0014-4894","type":"print"}],"subject":[],"published":{"date-parts":[[2013,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A new set of primers directed to 18S rRNA gene for molecular identification of Cryptosporidium spp. and their performance in the detection and differentiation of oocysts shed by synanthropic rodents","name":"articletitle","label":"Article Title"},{"value":"Experimental Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exppara.2013.09.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}