{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T01:31:45Z","timestamp":1783474305131,"version":"3.55.0"},"reference-count":37,"publisher":"Elsevier BV","content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Parasitology"],"published-print":{"date-parts":[[2014,5]]},"DOI":"10.1016\/j.exppara.2014.03.013","type":"journal-article","created":{"date-parts":[[2014,3,17]],"date-time":"2014-03-17T16:30:38Z","timestamp":1395073838000},"page":"33-38","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":20,"special_numbering":"C","title":["Trypanosoma cruzi chemical proteomics using immobilized benznidazole"],"prefix":"10.1016","volume":"140","author":[{"given":"Andrea","family":"Trochine","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guzm\u00e1n","family":"Alvarez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Corre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Faral-Tello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosario","family":"Dur\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos I.","family":"Batthyany","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo","family":"Cerecetto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercedes","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Robello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.exppara.2014.03.013_b0005","doi-asserted-by":"crossref","first-page":"2357","DOI":"10.1021\/pr700659m","article-title":"Proteomic analysis of Trypanosoma cruzi resistance to Benznidazole","volume":"7","author":"Andrade","year":"2008","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.exppara.2014.03.013_b0010","first-page":"721","article-title":"Evaluation of chemotherapy with benznidazole and nifurtimox in mice infected with Trypanosoma cruzi strains of different types","volume":"63","author":"Andrade","year":"1985","journal-title":"Bull. World Health Organ."},{"key":"10.1016\/j.exppara.2014.03.013_b0015","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.molbiopara.2010.11.010","article-title":"Genome-wide RNAi screens in African trypanosomes identify the nifurtimox activator NTR and the eflornithine transporter AAT6","volume":"176","author":"Baker","year":"2011","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.exppara.2014.03.013_b0020","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1016\/0092-8674(94)90138-4","article-title":"COPII: a membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum","volume":"77","author":"Barlowe","year":"1994","journal-title":"Cell"},{"key":"10.1016\/j.exppara.2014.03.013_b0025","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/S0166-6851(00)00302-9","article-title":"Identification of six Trypanosoma cruzi lineages by sequence-characterised amplified region markers","volume":"111","author":"Brisse","year":"2000","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.exppara.2014.03.013_b0030","first-page":"93","article-title":"Growth and differentiation in Trypanosoma Cruzi. I. Origin of metacyclic trypanosomes in liquid media","volume":"6","author":"Camargo","year":"1964","journal-title":"Rev. Inst. Med. Trop. Sao Paulo"},{"key":"10.1016\/j.exppara.2014.03.013_b0035","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1590\/S0074-02761988000100016","article-title":"Biological aspects of the Dm 28c clone of Trypanosoma cruzi after metacyclogenesis in chemically defined media","volume":"83","author":"Contreras","year":"1988","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"10.1016\/j.exppara.2014.03.013_b0040","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1007\/BF01941187","article-title":"Interaction of benznidazole reactive metabolites with nuclear and kinetoplastic DNA, proteins and lipids from Trypanosoma cruzi","volume":"44","author":"Diaz de Toranzo","year":"1988","journal-title":"Experientia"},{"issue":"Suppl. 1","key":"10.1016\/j.exppara.2014.03.013_b0045","first-page":"10","article-title":"Mechanism of the trypanocidal action of nifurtimox and other nitro-derivatives on Trypanosoma cruzi","volume":"40","author":"Docampo","year":"1980","journal-title":"Medicina (B Aires)"},{"key":"10.1016\/j.exppara.2014.03.013_b0050","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.molbiopara.2010.05.019","article-title":"Identification, cloning and characterization of an aldo-keto reductase from Trypanosoma cruzi with quinone oxido-reductase activity","volume":"173","author":"Garavaglia","year":"2010","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.exppara.2014.03.013_b0055","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1021\/jm049368n","article-title":"3-Hydroxymethyl-7-(N-substituted aminosulfonyl)-1,2,3,4-tetrahydroisoquinoline inhibitors of phenylethanolamine N-methyltransferase that display remarkable potency and selectivity","volume":"48","author":"Grunewald","year":"2005","journal-title":"J. Med. Chem."},{"key":"10.1016\/j.exppara.2014.03.013_b0060","doi-asserted-by":"crossref","first-page":"13088","DOI":"10.1074\/jbc.M111.230847","article-title":"Nifurtimox activation by trypanosomal type I nitroreductases generates cytotoxic nitrile metabolites","volume":"286","author":"Hall","year":"2011","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.exppara.2014.03.013_b0065","doi-asserted-by":"crossref","first-page":"5821","DOI":"10.1128\/AAC.01227-12","article-title":"Targeting the substrate preference of a type I nitroreductase to develop antitrypanosomal quinone-based prodrugs","volume":"56","author":"Hall","year":"2012","journal-title":"Antimicrob. Agents Chemother"},{"key":"10.1016\/j.exppara.2014.03.013_b0070","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1128\/AAC.05135-11","article-title":"Activation of benznidazole by trypanosomal type I nitroreductases results in glyoxal formation","volume":"56","author":"Hall","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.exppara.2014.03.013_b0075","doi-asserted-by":"crossref","first-page":"2188","DOI":"10.1021\/bi036046x","article-title":"Crystal structure of human prostaglandin F synthase (AKR1C3)","volume":"43","author":"Komoto","year":"2004","journal-title":"Biochemistry"},{"key":"10.1016\/j.exppara.2014.03.013_b0080","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1084\/jem.20020885","article-title":"A key role for old yellow enzyme in the metabolism of drugs by Trypanosoma cruzi","volume":"196","author":"Kubata","year":"2002","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.exppara.2014.03.013_b0085","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.exppara.2014.03.013_b0090","doi-asserted-by":"crossref","first-page":"41246","DOI":"10.1074\/jbc.C111.310847","article-title":"Extremely conserved ATP- or ADP-dependent enzymatic system for nicotinamide nucleotide repair","volume":"286","author":"Marbaix","year":"2011","journal-title":"J. Biol. Chem."},{"issue":"Suppl. 1","key":"10.1016\/j.exppara.2014.03.013_b0095","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1590\/S0074-02762009000900042","article-title":"The BENEFIT trial: testing the hypothesis that trypanocidal therapy is beneficial for patients with chronic Chagas heart disease","volume":"104","author":"Marin-Neto","year":"2009","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"10.1016\/j.exppara.2014.03.013_b0100","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1016\/j.cbpa.2006.03.004","article-title":"Mode of action of natural and synthetic drugs against Trypanosoma cruzi and their interaction with the mammalian host","volume":"146","author":"Maya","year":"2007","journal-title":"Comp. Biochem. Physiol. A: Mol. Integr. Physiol."},{"key":"10.1016\/j.exppara.2014.03.013_b0105","first-page":"101","article-title":"Effects of nifurtimox and benznidazole upon glutathione and trypanothione content in epimastigote, trypomastigote and amastigote forms of Trypanosoma cruzi","volume":"86","author":"Maya","year":"1997","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.exppara.2014.03.013_b0110","doi-asserted-by":"crossref","first-page":"255","DOI":"10.2174\/13894501113146660218","article-title":"Proteomic and Bioinformatic Analysis of Trypanosoma Cruzi Chemotherapy and Potential Drug Targets: New Pieces for an Old Puzzle","volume":"15","author":"Menna-Barreto","year":"2014","journal-title":"Curr. Drug Targets"},{"key":"10.1016\/j.exppara.2014.03.013_b0115","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.molbiopara.2005.12.001","article-title":"Deletion of copies of the gene encoding old yellow enzyme (TcOYE), a NAD(P)H flavin oxidoreductase, associates with in vitro-induced benznidazole resistance in Trypanosoma cruzi","volume":"146","author":"Murta","year":"2006","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.exppara.2014.03.013_b0120","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1002\/pmic.200300637","article-title":"Proteomic analysis of the human pathogen Trypanosoma cruzi","volume":"4","author":"Paba","year":"2004","journal-title":"Proteomics"},{"key":"10.1016\/j.exppara.2014.03.013_b0125","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1016\/j.ijpara.2004.05.002","article-title":"Proteome analysis of the causative agent of Chagas disease: Trypanosoma cruzi","volume":"34","author":"Parodi-Talice","year":"2004","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.exppara.2014.03.013_b0130","doi-asserted-by":"crossref","first-page":"1422","DOI":"10.1002\/jms.1267","article-title":"Proteomic analysis of metacyclic trypomastigotes undergoing Trypanosoma cruzi metacyclogenesis","volume":"42","author":"Parodi-Talice","year":"2007","journal-title":"J. Mass Spectrom."},{"key":"10.1016\/j.exppara.2014.03.013_b0135","doi-asserted-by":"crossref","first-page":"3551","DOI":"10.1002\/(SICI)1522-2683(19991201)20:18<3551::AID-ELPS3551>3.0.CO;2-2","article-title":"Probability-based protein identification by searching sequence databases using mass spectrometry data","volume":"20","author":"Perkins","year":"1999","journal-title":"Electrophoresis"},{"key":"10.1016\/j.exppara.2014.03.013_b0140","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1016\/S0140-6736(10)60061-X","article-title":"Chagas disease","volume":"375","author":"Rassi","year":"2010","journal-title":"Lancet"},{"key":"10.1016\/j.exppara.2014.03.013_b9005","series-title":"Disease Control Priorities in Developing Countries","first-page":"433","article-title":"Tropical Diseases Targeted for Elimination: Chagas Disease, Lymphatic Filariasis, Onchocerciasis, and Leprosy","author":"Remme","year":"2006"},{"key":"10.1016\/j.exppara.2014.03.013_b0150","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1016\/j.pt.2006.09.011","article-title":"The future of Chagas disease control","volume":"22","author":"Schofield","year":"2006","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.exppara.2014.03.013_b0155","doi-asserted-by":"crossref","first-page":"4739","DOI":"10.1091\/mbc.E09-07-0542","article-title":"Streamlined architecture and glycosylphosphatidylinositol-dependent trafficking in the early secretory pathway of African trypanosomes","volume":"20","author":"Sevova","year":"2009","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.exppara.2014.03.013_b0160","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1038\/nrd2410","article-title":"Target deconvolution strategies in drug discovery","volume":"6","author":"Terstappen","year":"2007","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.exppara.2014.03.013_b0165","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.actatropica.2009.10.023","article-title":"Specific chemotherapy of Chagas disease: relevance, current limitations and new approaches","volume":"115","author":"Urbina","year":"2010","journal-title":"Acta Trop."},{"key":"10.1016\/j.exppara.2014.03.013_b0170","doi-asserted-by":"crossref","first-page":"2072","DOI":"10.2174\/156802611796575894","article-title":"Trypanocidal activity of nitroaromatic prodrugs: current treatments and future perspectives","volume":"11","author":"Wilkinson","year":"2011","journal-title":"Curr. Top. Med. Chem."},{"key":"10.1016\/j.exppara.2014.03.013_b0175","doi-asserted-by":"crossref","first-page":"e31","DOI":"10.1017\/S1462399409001252","article-title":"Trypanocidal drugs: mechanisms, resistance and new targets","volume":"11","author":"Wilkinson","year":"2009","journal-title":"Expert Rev. Mol. Med."},{"key":"10.1016\/j.exppara.2014.03.013_b0180","doi-asserted-by":"crossref","first-page":"5022","DOI":"10.1073\/pnas.0711014105","article-title":"A mechanism for cross-resistance to nifurtimox and benznidazole in trypanosomes","volume":"105","author":"Wilkinson","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1016\/j.exppara.2014.03.013_b0185","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1107\/S0909049510033595","article-title":"Structure of the inhibitor complex of old yellow enzyme from Trypanosoma cruzi","volume":"18","author":"Yamaguchi","year":"2011","journal-title":"J. Synchrotron Radiat."}],"container-title":["Experimental Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489414000472?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489414000472?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,10,6]],"date-time":"2018-10-06T01:54:33Z","timestamp":1538790873000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014489414000472"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,5]]},"references-count":37,"alternative-id":["S0014489414000472"],"URL":"https:\/\/doi.org\/10.1016\/j.exppara.2014.03.013","relation":{},"ISSN":["0014-4894"],"issn-type":[{"value":"0014-4894","type":"print"}],"subject":[],"published":{"date-parts":[[2014,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Trypanosoma cruzi chemical proteomics using immobilized benznidazole","name":"articletitle","label":"Article Title"},{"value":"Experimental Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exppara.2014.03.013","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}