{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T00:39:49Z","timestamp":1772843989387,"version":"3.50.1"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T00:00:00Z","timestamp":1459468800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100005156","name":"Alexander von Humboldt-Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005156","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["01KI0765"],"award-info":[{"award-number":["01KI0765"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["01KI1002F"],"award-info":[{"award-number":["01KI1002F"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["Toxonet01"],"award-info":[{"award-number":["Toxonet01"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["Toxonet02"],"award-info":[{"award-number":["Toxonet02"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006181","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado da Bahia","doi-asserted-by":"publisher","award":["PET0003\/2012"],"award-info":[{"award-number":["PET0003\/2012"]}],"id":[{"id":"10.13039\/501100006181","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Parasitology"],"published-print":{"date-parts":[[2016,4]]},"DOI":"10.1016\/j.exppara.2016.01.014","type":"journal-article","created":{"date-parts":[[2016,2,4]],"date-time":"2016-02-04T18:48:36Z","timestamp":1454611716000},"page":"46-56","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"special_numbering":"C","title":["Characterization of an IgG monoclonal antibody targeted to both tissue cyst and sporocyst walls of Toxoplasma gondii"],"prefix":"10.1016","volume":"163","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3741-121X","authenticated-orcid":false,"given":"Lu\u00eds F.P.","family":"Gondim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Wolf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Majda G.","family":"Vrhovec","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikola","family":"Pantchev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Bauer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin C.","family":"Langenmayer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Bohne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens P.","family":"Teifke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jitender P.","family":"Dubey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franz J.","family":"Conraths","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gereon","family":"Schares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.exppara.2016.01.014_bib1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1111\/j.1550-7408.1984.tb04304.x","article-title":"Antigenic similarity between the coccidian parasites Toxoplasma gondii and Hammondia hammondi","volume":"31","author":"Araujo","year":"1984","journal-title":"J.\u00a0Protozool."},{"key":"10.1016\/j.exppara.2016.01.014_bib2","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1016\/j.pt.2006.07.004","article-title":"The coccidian oocyst: a tough nut to crack!","volume":"22","author":"Belli","year":"2006","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.exppara.2016.01.014_bib3","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1136\/vr.112.5.98","article-title":"Simple technique for the direct isolation of Toxoplasma tissue cysts from fetal ovine brain","volume":"112","author":"Blewett","year":"1983","journal-title":"Vet. Rec."},{"key":"10.1016\/j.exppara.2016.01.014_bib4","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1128\/EC.05182-11","article-title":"Identification of tissue cyst wall components by transcriptome analysis of in\u00a0vivo and in\u00a0vitro Toxoplasma gondii bradyzoites","volume":"10","author":"Buchholz","year":"2011","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.exppara.2016.01.014_bib5","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00258-12","article-title":"\u03b2-1,3-glucan, which can be targeted by drugs, forms a trabecular scaffold in the oocyst walls of Toxoplasma and Eimeria","volume":"3","author":"Bushkin","year":"2012","journal-title":"MBio"},{"key":"10.1016\/j.exppara.2016.01.014_bib6","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1016\/S0020-7519(98)00023-X","article-title":"Advances in the life cycle of Toxoplasma gondii","volume":"28","author":"Dubey","year":"1998","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.exppara.2016.01.014_bib7","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0304-4017(97)00135-0","article-title":"Refinement of pepsin digestion method for isolation of Toxoplasma gondii from infected tissues","volume":"74","author":"Dubey","year":"1998","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.exppara.2016.01.014_bib8","series-title":"Toxoplasmosis of Animals and Humans","author":"Dubey","year":"2010"},{"key":"10.1016\/j.exppara.2016.01.014_bib9","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1645\/12-128.1","article-title":"Development of early tissue cysts and associated pathology of Besnoitia besnoiti in a naturally infected bull (Bos taurus) from South Africa","volume":"99","author":"Dubey","year":"2013","journal-title":"J.\u00a0Parasitol."},{"key":"10.1016\/j.exppara.2016.01.014_bib10","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.mimet.2004.11.024","article-title":"Immunomagnetic separation of Toxoplasma gondii oocysts using a monoclonal antibody directed against the oocyst wall","volume":"61","author":"Dum\u00e8tre","year":"2005","journal-title":"J.\u00a0Microbiol. Methods"},{"key":"10.1016\/j.exppara.2016.01.014_bib11","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1007\/s00436-007-0573-0","article-title":"Detection of Toxoplasma gondii in water by an immunomagnetic separation method targeting the sporocysts","volume":"101","author":"Dum\u00e8tre","year":"2007","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.exppara.2016.01.014_bib12","doi-asserted-by":"crossref","first-page":"11535","DOI":"10.1073\/pnas.1308425110","article-title":"Mechanics of the Toxoplasma gondii oocyst wall","volume":"110","author":"Dum\u00e8tre","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.exppara.2016.01.014_bib13","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1078\/S1434-4610(04)70031-0","article-title":"Molecular phylogeny of Besnoitia and the genetic relationships among Besnoitia of cattle, wildebeest and goats","volume":"151","author":"Ellis","year":"2000","journal-title":"Protist"},{"key":"10.1016\/j.exppara.2016.01.014_bib14","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1111\/j.1550-7408.2002.tb00381.x","article-title":"Isolation of purified oocyst walls and sporocysts from Toxoplasma gondii","volume":"49","author":"Everson","year":"2002","journal-title":"J.\u00a0Eukaryot. Microbiol."},{"key":"10.1016\/j.exppara.2016.01.014_bib15","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.exppara.2004.03.014","article-title":"Massive excystation of Toxoplasma gondii sporozoites","volume":"107","author":"Freyre","year":"2004","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.exppara.2016.01.014_bib16","doi-asserted-by":"crossref","first-page":"e29955","DOI":"10.1371\/journal.pone.0029955","article-title":"Proteomic analysis of fractionated Toxoplasma oocysts reveals clues to their environmental resistance","volume":"7","author":"Fritz","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.exppara.2016.01.014_bib17","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.vetpar.2015.03.028","article-title":"In\u00a0vitro cultivation of Hammondia heydorni: generation of tachyzoites, stage conversion into bradyzoites, and evaluation of serologic cross-reaction with Neospora caninum","volume":"210","author":"Gondim","year":"2015","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.exppara.2016.01.014_bib18","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1128\/cdli.2.5.542-548.1995","article-title":"Monoclonal rat antibodies directed against Toxoplasma gondii suitable for studying tachyzoite-bradyzoite interconversion in\u00a0vivo","volume":"2","author":"Gross","year":"1995","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"10.1016\/j.exppara.2016.01.014_bib19","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1645\/GE-2782.1","article-title":"Identification of a sporozoite-specific antigen from Toxoplasma gondii","volume":"97","author":"Hill","year":"2011","journal-title":"J.\u00a0Parasitol."},{"key":"10.1016\/j.exppara.2016.01.014_bib20","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/256495a0","article-title":"Continuous cultures of fused cells secreting antibody of predefined specificity","volume":"256","author":"Kohler","year":"1975","journal-title":"Nature"},{"key":"10.1016\/j.exppara.2016.01.014_bib21","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1007\/s00436-014-4261-6","article-title":"Besnoitia besnoiti infection in cattle and mice: ultrastructural pathology in acute and chronic besnoitiosis","volume":"114","author":"Langenmayer","year":"2015","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.exppara.2016.01.014_bib22","doi-asserted-by":"crossref","first-page":"197S","DOI":"10.1111\/j.1550-7408.2001.tb00517.x","article-title":"Removal of Toxoplasma gondii oocysts from sea water by eastern oysters (Crassostrea virginica)","author":"Lindsay","year":"2001","journal-title":"J.\u00a0Eukaryot. Microbiol."},{"key":"10.1016\/j.exppara.2016.01.014_bib23","doi-asserted-by":"crossref","first-page":"354","DOI":"10.2307\/3284181","article-title":"An immunohistochemical method for detecting bradyzoite antigen (BAG5) in Toxoplasma gondii-infected tissues cross-reacts with a Neospora caninum bradyzoite antigen","volume":"82","author":"McAllister","year":"1996","journal-title":"J.\u00a0Parasitol."},{"key":"10.1016\/j.exppara.2016.01.014_bib24","doi-asserted-by":"crossref","first-page":"2850","DOI":"10.1128\/jcm.34.11.2850-2852.1996","article-title":"Diagnosis of Neospora caninum and Toxoplasma gondii infection by PCR and DNA hybridization immunoassay","volume":"34","author":"M\u00fcller","year":"1996","journal-title":"J.\u00a0Clin. Microbiol."},{"key":"10.1016\/j.exppara.2016.01.014_bib25","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1111\/j.1751-0813.1986.tb02886.x","article-title":"Serological cross-reactivity between Hammondia hammondi and Toxoplasma gondii in experimentally inoculated sheep","volume":"63","author":"Munday","year":"1986","journal-title":"Aust. Vet. J."},{"key":"10.1016\/j.exppara.2016.01.014_bib26","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/0166-6851(94)90155-4","article-title":"Molecular characterization of a 65-kilodalton Toxoplasma gondii antigen expressed abundantly in the matrix of tissue cysts","volume":"66","author":"Parmley","year":"1994","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.exppara.2016.01.014_bib27","doi-asserted-by":"crossref","first-page":"1639","DOI":"10.1016\/j.ijpara.2010.06.009","article-title":"Molecular characterisation of a novel family of cysteine-rich proteins of Toxoplasma gondii and ultrastructural evidence of oocyst wall localisation","volume":"40","author":"Possenti","year":"2010","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.exppara.2016.01.014_bib28","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1186\/1471-2164-14-183","article-title":"Global proteomic analysis of the oocyst\/sporozoite of Toxoplasma gondii reveals commitment to a host-independent lifestyle","volume":"14","author":"Possenti","year":"2013","journal-title":"BMC Genom."},{"key":"10.1016\/j.exppara.2016.01.014_bib29","doi-asserted-by":"crossref","first-page":"1362","DOI":"10.1645\/0022-3395(2000)086[1362:MATHHA]2.0.CO;2","article-title":"Monoclonal antibodies to Hammondia hammondi allowing immunological differentiation from Toxoplasma gondii","volume":"86","author":"Riahi","year":"2000","journal-title":"J.\u00a0Parasitol."},{"key":"10.1016\/j.exppara.2016.01.014_bib30","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.mcp.2008.04.003","article-title":"Characterization of a repetitive DNA fragment in Hammondia hammondi and its utility for the specific differentiation of H.\u00a0hammondi from Toxoplasma gondii by PCR","volume":"22","author":"Schares","year":"2008","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.exppara.2016.01.014_bib31","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1053\/tvjl.2001.0599","article-title":"Coprodiagnosis of Hammondia heydorni in dogs by PCR based amplification of ITS 1 rRNA: differentiation from morphologically indistinguishable oocysts of Neospora caninum","volume":"163","author":"\u015clapeta","year":"2002","journal-title":"Vet. J."},{"key":"10.1016\/j.exppara.2016.01.014_bib32","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1006\/expr.1994.1039","article-title":"Experimental induction of bradyzoite-specific antigen expression and cyst formation by the RH strain of Toxoplasma gondii in\u00a0vitro","volume":"78","author":"Soete","year":"1994","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.exppara.2016.01.014_bib33","doi-asserted-by":"crossref","first-page":"2131","DOI":"10.1128\/JCM.00128-09","article-title":"Contributions of immunoblotting, real-time PCR, and the Goldmann-Witmer coefficient to diagnosis of atypical toxoplasmic retinochoroiditis","volume":"47","author":"Talabani","year":"2009","journal-title":"J.\u00a0Clin. Microbiol."},{"key":"10.1016\/j.exppara.2016.01.014_bib34","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1016\/S0020-7519(00)00124-7","article-title":"Toxoplasma gondii: from animals to humans","volume":"30","author":"Tenter","year":"2000","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.exppara.2016.01.014_bib35","doi-asserted-by":"crossref","first-page":"e1003823","DOI":"10.1371\/journal.ppat.1003823","article-title":"The Toxoplasma gondii cyst wall protein CST1 is critical for cyst wall integrity and promotes bradyzoite persistence","volume":"9","author":"Tomita","year":"2013","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.exppara.2016.01.014_bib36","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1093\/infdis\/166.1.213","article-title":"Identification of Toxoplasma gondii bradyzoite-specific monoclonal antibodies","volume":"166","author":"Weiss","year":"1992","journal-title":"J.\u00a0Infect. Dis."},{"key":"10.1016\/j.exppara.2016.01.014_bib37","article-title":"A\u00a0purification method for Enrichment of the Toxoplasma gondii cyst Wall","volume":"1","author":"Zhang","year":"2010","journal-title":"J.\u00a0Neuroparasitol."},{"key":"10.1016\/j.exppara.2016.01.014_bib38","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1128\/IAI.69.1.501-507.2001","article-title":"Initial characterization of CST1, a Toxoplasma gondii cyst wall glycoprotein","volume":"69","author":"Zhang","year":"2001","journal-title":"Infect. Immun."}],"container-title":["Experimental Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489416300145?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489416300145?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T02:57:45Z","timestamp":1655953065000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014489416300145"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4]]},"references-count":38,"alternative-id":["S0014489416300145"],"URL":"https:\/\/doi.org\/10.1016\/j.exppara.2016.01.014","relation":{},"ISSN":["0014-4894"],"issn-type":[{"value":"0014-4894","type":"print"}],"subject":[],"published":{"date-parts":[[2016,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Characterization of an IgG monoclonal antibody targeted to both tissue cyst and sporocyst walls of Toxoplasma gondii","name":"articletitle","label":"Article Title"},{"value":"Experimental Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exppara.2016.01.014","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2016 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}