{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:28:16Z","timestamp":1760646496941},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Sunway University, Malaysia"},{"DOI":"10.13039\/501100004681","name":"Higher Education Commission, Pakistan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004681","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Parasitology"],"published-print":{"date-parts":[[2016,9]]},"DOI":"10.1016\/j.exppara.2016.06.006","type":"journal-article","created":{"date-parts":[[2016,6,20]],"date-time":"2016-06-20T07:53:22Z","timestamp":1466409202000},"page":"16-24","update-policy":"http:\/\/dx.doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"special_numbering":"C","title":["The effect of peptidic and non-peptidic proteasome inhibitors on the biological properties of Acanthamoeba castellanii belonging to the T4 genotype"],"prefix":"10.1016","volume":"168","author":[{"given":"Ruqaiyyah","family":"Siddiqui","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sahreena","family":"Saleem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naveed Ahmed","family":"Khan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.exppara.2016.06.006_bib1","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1007\/s002390010117","article-title":"Phylogenomic analysis of the alpha proteasome gene family from early-diverging eukaryotes","volume":"51","author":"Bouzat","year":"2000","journal-title":"J.\u00a0Mol. Evol."},{"key":"10.1016\/j.exppara.2016.06.006_bib2","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/0092-8674(94)90396-4","article-title":"The ubiquitin-proteasome proteolytic pathway","volume":"79","author":"Ciechanover","year":"1994","journal-title":"Cell"},{"key":"10.1016\/j.exppara.2016.06.006_bib3","doi-asserted-by":"crossref","first-page":"13437","DOI":"10.1074\/jbc.272.20.13437","article-title":"Lactacystin and clasto-lactacystin \u03b2-lactone modify multiple proteasome \u03b2-subunits and inhibit intracellular protein degradation and major histocompatibility complex class I antigen presentation","volume":"272","author":"Craiu","year":"1997","journal-title":"Biol. Chem."},{"key":"10.1016\/j.exppara.2016.06.006_bib4","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1096\/fasebj.1.5.2824267","article-title":"Molecular determinants of protein half-lives in eukaryotic cells","volume":"1","author":"Dice","year":"1987","journal-title":"FASEB J."},{"key":"10.1016\/j.exppara.2016.06.006_bib5","doi-asserted-by":"crossref","first-page":"7273","DOI":"10.1074\/jbc.271.13.7273","article-title":"Mechanistic studies on the inactivation of the proteasome by lactacysting","volume":"271","author":"Dick","year":"1996","journal-title":"Biol. Chem."},{"key":"10.1016\/j.exppara.2016.06.006_bib6","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.actatropica.2005.05.004","article-title":"Acanthamoeba isolates belonging to T1, T2, T3, T4 but not T7 encyst in response to increased osmolarity and cysts do not bind to human corneal epithelial cells","volume":"95","author":"Dudley","year":"2005","journal-title":"Acta Trop."},{"key":"10.1016\/j.exppara.2016.06.006_bib7","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/j.1574-6968.2008.01249.x","article-title":"The role of proteases in the differentiation of Acanthamoeba castellanii","volume":"286","author":"Dudley","year":"2008","journal-title":"FEMS Microb. Lett."},{"key":"10.1016\/j.exppara.2016.06.006_bib8","first-page":"857","article-title":"Apoptosis induced in hepatoblastoma HepG2 cells by the proteasome inhibitor MG 132 is associated with hydrogen peroxide production, expression of Bcl-Xs and activation of caspase-3","volume":"21","author":"Emanuele","year":"2002","journal-title":"Int. J. Oncol."},{"key":"10.1016\/j.exppara.2016.06.006_bib9","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1126\/science.7732382","article-title":"Inhibition of proteasome activities and subunit-specific aminoterminal threonine modification by lactacystin","volume":"268","author":"Fenteany","year":"1995","journal-title":"Science"},{"key":"10.1016\/j.exppara.2016.06.006_bib10","doi-asserted-by":"crossref","first-page":"2731","DOI":"10.1128\/AAC.42.10.2731","article-title":"Proteasome inhibitors block development of Plasmodium spp.","volume":"42","author":"Gantt","year":"1998","journal-title":"Antimicrob. Agents Chem."},{"key":"10.1016\/j.exppara.2016.06.006_bib11","doi-asserted-by":"crossref","DOI":"10.1152\/physrev.00027.2001","article-title":"The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction","volume":"82","author":"Glickman","year":"2002","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.exppara.2016.06.006_bib12","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1126\/science.7725095","article-title":"Functions of the proteasome: the lysis at the end of the tunnel","volume":"268","author":"Goldberg","year":"1995","journal-title":"Science"},{"key":"10.1016\/j.exppara.2016.06.006_bib13","doi-asserted-by":"crossref","first-page":"1909","DOI":"10.1084\/jem.184.5.1909","article-title":"Proteasome activity is required for the stage-specific transformation of a protozoan parasite","volume":"184","author":"Gonzalez","year":"1996","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.exppara.2016.06.006_bib14","first-page":"S139","article-title":"Proteasome function id required for encystation of Entamoeba invadens","volume":"28","author":"Gonzalez","year":"1997","journal-title":"Arch. Med. Res."},{"key":"10.1016\/j.exppara.2016.06.006_bib15","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1128\/mr.56.4.592-621.1992","article-title":"Regulation by proteolysis: energy dependent proteases and their targets","volume":"56","author":"Gottesman","year":"1992","journal-title":"Microbiol. Rev."},{"key":"10.1016\/j.exppara.2016.06.006_bib16","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1016\/j.sbi.2003.10.005","article-title":"Molecular shredders: how proteasomes fulfill their role","volume":"13","author":"Groll","year":"2003","journal-title":"Curr. Opin. Struct. Biol."},{"key":"10.1016\/j.exppara.2016.06.006_bib17","first-page":"215","article-title":"The effect of MG 132, a proteasome inhibitor on HeLa cells in relation to cell growth, reactive oxygen species and GSH","volume":"22","author":"Han","year":"2009","journal-title":"Oncol. Rep."},{"key":"10.1016\/j.exppara.2016.06.006_bib18","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/0955-0674(95)80031-X","article-title":"Ubiquitin, proteasomes, and the regulation of intracellular protein degradation","volume":"7","author":"Hochstrasser","year":"1995","journal-title":"Curr. Opin. Cell Biol."},{"key":"10.1016\/j.exppara.2016.06.006_bib19","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1111\/j.1574-6976.2006.00023.x","article-title":"Acanthamoeba: biology and increasing importance in human health","volume":"30","author":"Khan","year":"2006","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.exppara.2016.06.006_bib20","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.1016\/j.ijpara.2009.06.004","article-title":"Acanthamoeba affects the integrity of human brain microvascular endothelial cells and degrades the tight junction proteins","volume":"39","author":"Khan","year":"2009","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.exppara.2016.06.006_bib21","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/S0163-4453(98)93054-7","article-title":"Acanthamoeba castellanii: growth, encystment, excystment and biocide susceptibility","volume":"36","author":"Khunkitti","year":"1998","journal-title":"J.\u00a0Infect."},{"key":"10.1016\/j.exppara.2016.06.006_bib22","doi-asserted-by":"crossref","first-page":"27280","DOI":"10.1074\/jbc.271.44.27280","article-title":"Selective inhibitors of the proteasome-dependent and vacuolar pathways of protein degradation in Saccharomyces cerevisiae","volume":"271","author":"Lee","year":"1996","journal-title":"Biol. Chem."},{"key":"10.1016\/j.exppara.2016.06.006_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/S0962-8924(98)01346-4","article-title":"Proteasome inhibitors: valuable new tools for cell biologists","volume":"8","author":"Lee","year":"1998","journal-title":"Trend. Cell Biol."},{"key":"10.1016\/j.exppara.2016.06.006_bib24","first-page":"841","article-title":"PS-341, a novel proteasome inhibitor, induces Bcl-2 phosphorylation and cleavage in association with G2-M phase arrest and apoptosis","volume":"1","author":"Ling","year":"2002","journal-title":"Mol. Cancer Ther."},{"key":"10.1016\/j.exppara.2016.06.006_bib25","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1007\/s00436-002-0601-z","article-title":"Effect of proteasome inhibitors on the growth, encystation, and excystation of Entamoeba histolytica and Entamoeba invadens","volume":"88","author":"Makioka","year":"2002","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.exppara.2016.06.006_bib26","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1128\/CMR.16.2.273-307.2003","article-title":"Acanthamoeba spp. as agents of disease in humans","volume":"16","author":"Marciano-Cabral","year":"2003","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.exppara.2016.06.006_bib27","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1111\/j.1750-3639.1997.tb01076.x","article-title":"Free-living, amphizoic and opportunistic amebas","volume":"7","author":"Martinez","year":"1997","journal-title":"Brain Pathol."},{"key":"10.1016\/j.exppara.2016.06.006_bib28","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1111\/j.1574-695X.2006.00065.x","article-title":"Balamuthia mandrillaris exhibits matalloprotease activities","volume":"47","author":"Matin","year":"2006","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"10.1016\/j.exppara.2016.06.006_bib29","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.exppara.2008.01.001","article-title":"Acanthamoeba castellanii: gene profile of encystation by ESTs analysis and KOG assignment","volume":"119","author":"Moon","year":"2008","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.exppara.2016.06.006_bib30","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/S0166-6851(97)00197-7","article-title":"Inhibition of proteasome activity blocks cell cycle progression at specific phase boundaries in african trypanosomes","volume":"90","author":"Mutomba","year":"1997","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.exppara.2016.06.006_bib31","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/S0166-6851(98)00012-7","article-title":"The role of proteolysis during differentiation of Trypanosoma brucei from the bloodstream to the procyclic form","volume":"93","author":"Mutomba","year":"1998","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.exppara.2016.06.006_bib32","doi-asserted-by":"crossref","first-page":"113","DOI":"10.7164\/antibiotics.44.113","article-title":"Lactacystin, a novel microbial metabolite, induces neuritogenesis of neuroblastoma cells","volume":"44","author":"Omura","year":"1991","journal-title":"J.\u00a0Antibiot."},{"key":"10.1016\/j.exppara.2016.06.006_bib33","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/S1471-4922(02)00064-8","article-title":"Characterization and role of protozoan parasite proteasomes","volume":"19","author":"Paugam","year":"2003","journal-title":"Trend. Parasitol."},{"key":"10.1016\/j.exppara.2016.06.006_bib34","doi-asserted-by":"crossref","first-page":"2029","DOI":"10.1158\/1535-7163.MCT-11-0745","article-title":"Bortezomib: understanding the mechanism of action","volume":"10","author":"Piperdi","year":"2011","journal-title":"Mol. Cancer Ther."},{"key":"10.1016\/j.exppara.2016.06.006_bib35","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1126\/science.1212868","article-title":"Host proteasomal degradation generates amino acids essential for intracellular bacterial growth","volume":"334","author":"Price","year":"2011","journal-title":"Science"},{"key":"10.1016\/j.exppara.2016.06.006_bib36","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/S0166-6851(96)02770-3","article-title":"The Entamoeba histolytica proteasome \u03b1-subunit gene","volume":"84","author":"Ramos","year":"1997","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.exppara.2016.06.006_bib37","doi-asserted-by":"crossref","first-page":"6212","DOI":"10.1074\/jbc.271.11.6212","article-title":"Evidence for the existence of both proteasomes and a novel high molecular weight peptidae in Entamoeba histolytica","volume":"271","author":"Scholze","year":"1996","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.exppara.2016.06.006_bib38","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1017\/S0031182099006071","article-title":"Proteasome inhibitors block intracellular growth and replication of Toxoplasma gondii","volume":"121","author":"Shaw","year":"2000","journal-title":"Parasitol"},{"key":"10.1016\/j.exppara.2016.06.006_bib39","doi-asserted-by":"crossref","first-page":"2704","DOI":"10.1128\/IAI.73.5.2704-2708.2005","article-title":"Acanthamoeba castellanii induces host cell death via a phosphatidylinositol 3-kinase-dependent mechanism","volume":"73","author":"Sissons","year":"2005","journal-title":"Infect. Immun."},{"key":"10.1016\/j.exppara.2016.06.006_bib40","doi-asserted-by":"crossref","first-page":"2595","DOI":"10.1128\/JCM.00144-06","article-title":"Use of in\u00a0vitro assays to determine effects of human serum on biological characteristics of Acanthamoeba castellanii","volume":"44","author":"Sissons","year":"2006","journal-title":"J.\u00a0Clin. Microbiol."},{"key":"10.1016\/j.exppara.2016.06.006_bib41","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/S0165-5728(97)00036-2","article-title":"Selective expression of adhesion molecules on human brain microvascular endothelial cells","volume":"76","author":"Stins","year":"1997","journal-title":"Neuroimmunol"},{"key":"10.1016\/j.exppara.2016.06.006_bib42","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/jac\/46.1.27","article-title":"Emergence of resistance to biocides during differentiation of Acanthamoeba castellanii","volume":"46","author":"Turner","year":"2000","journal-title":"J.\u00a0Antimicrob. Chem."},{"key":"10.1016\/j.exppara.2016.06.006_bib43","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1146\/annurev.biochem.68.1.1015","article-title":"The 26S proteasome: a molecular machine designed for controlled proteolysis","volume":"68","author":"Voges","year":"1999","journal-title":"Ann. Rev. Biochem."},{"key":"10.1016\/j.exppara.2016.06.006_bib44","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1146\/annurev.mi.30.100176.001201","article-title":"Differentiation in Acanthamoeba castellanii","volume":"30","author":"Weisman","year":"1976","journal-title":"Ann. Rev. Microbiol."}],"container-title":["Experimental Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489416301187?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489416301187?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T19:48:51Z","timestamp":1656704931000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014489416301187"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9]]},"references-count":44,"alternative-id":["S0014489416301187"],"URL":"https:\/\/doi.org\/10.1016\/j.exppara.2016.06.006","relation":{},"ISSN":["0014-4894"],"issn-type":[{"value":"0014-4894","type":"print"}],"subject":[],"published":{"date-parts":[[2016,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The effect of peptidic and non-peptidic proteasome inhibitors on the biological properties of Acanthamoeba castellanii belonging to the T4 genotype","name":"articletitle","label":"Article Title"},{"value":"Experimental Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exppara.2016.06.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}