{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T17:43:24Z","timestamp":1792086204489,"version":"4.4.0"},"reference-count":73,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,11,1]],"date-time":"2016-11-01T00:00:00Z","timestamp":1477958400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,11,1]],"date-time":"2016-11-01T00:00:00Z","timestamp":1477958400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2017,11,2]],"date-time":"2017-11-02T00:00:00Z","timestamp":1509580800000},"content-version":"am","delay-in-days":366,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100010679","name":"Pasteur Institute of Iran","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100010679","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Iran National Science FoundationIran National Science Foundation (INSF)","award":["93004137"],"award-info":[{"award-number":["93004137"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Parasitology"],"published-print":{"date-parts":[[2016,11]]},"DOI":"10.1016\/j.exppara.2016.09.008","type":"journal-article","created":{"date-parts":[[2016,9,20]],"date-time":"2016-09-20T23:16:52Z","timestamp":1474413412000},"page":"236-246","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":29,"special_numbering":"C","title":["Synergistic effect of rSAG1 and rGRA2 antigens formulated in PLGA microspheres in eliciting immune protection against Toxoplasama gondii"],"prefix":"10.1016","volume":"170","author":[{"given":"Mojgan","family":"Allahyari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reyhaneh","family":"Mohabati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samira","family":"Amiri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad Reza","family":"Esmaeili Rastaghi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jalal","family":"Babaie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehdi","family":"Mahdavi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alireza","family":"Vatanara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Majid","family":"Golkar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.exppara.2016.09.008_bib1","series-title":"Immunological Control of Toxoplasma Gondii and Appropriate Vaccine Design, Toxoplasma Gondii","first-page":"183","author":"Alexander","year":"1996"},{"issue":"3","key":"10.1016\/j.exppara.2016.09.008_bib2","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1080\/21645515.2015.1102804","article-title":"Peptide\/protein vaccine delivery system based on PLGA particles","volume":"12","author":"Allahyari","year":"2016","journal-title":"Hum. Vaccines Immunother."},{"key":"10.1016\/j.exppara.2016.09.008_bib3","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.jim.2014.11.012","article-title":"Production of in-vitro refolded and highly antigenic SAG1 for development of a sensitive and specific Toxoplasma IgG ELISA","volume":"416","author":"Allahyari","year":"2015","journal-title":"J.\u00a0Immunol. Methods"},{"key":"10.1016\/j.exppara.2016.09.008_bib4","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1038\/nri2868","article-title":"Vaccine delivery: a matter of size, geometry, kinetics and molecular patterns","volume":"10","author":"Bachmann","year":"2010","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.exppara.2016.09.008_bib5","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/j.jconrel.2014.06.027","article-title":"Designing and building the next generation of improved vaccine adjuvants","volume":"190","author":"Brito","year":"2014","journal-title":"J.\u00a0Control Release"},{"key":"10.1016\/j.exppara.2016.09.008_bib6","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1016\/j.vaccine.2003.10.020","article-title":"Potent, long lasting systemic antibody levels and mixed Th1\/Th2 immune response after nasal immunization with malaria antigen loaded PLGA microparticles","volume":"22","author":"Carcaboso","year":"2004","journal-title":"Vaccine"},{"key":"10.1016\/j.exppara.2016.09.008_bib7","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2016.00609","article-title":"Evaluation of immunoprotection conferred by the subunit vaccines of GRA2 and GRA5 against acute Toxoplasmosis in BALB\/c mice","volume":"7","author":"Ching","year":"2016","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.exppara.2016.09.008_bib8","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1016\/j.exppara.2013.04.002","article-title":"Encapsulation of chimeric protein rSAG1\/2 into poly (lactide-co-glycolide) microparticles induces long-term protective immunity against Toxoplasma gondii in mice","volume":"134","author":"Chuang","year":"2013","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.exppara.2016.09.008_bib9","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1186\/1756-3305-6-34","article-title":"Induction of long-lasting protective immunity against Toxoplasma gondii in BALB\/c mice by recombinant surface antigen 1 protein encapsulated in poly (lactide-co-glycolide) microparticles","volume":"6","author":"Chuang","year":"2013","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.exppara.2016.09.008_bib10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1756-3305-6-63","article-title":"Compound DNA vaccine encoding SAG1\/SAG3 with A 2\/B subunit of cholera toxin as a genetic adjuvant protects BALB\/c mice against Toxoplasma gondii","volume":"6","author":"Cong","year":"2013","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.exppara.2016.09.008_bib11","doi-asserted-by":"crossref","first-page":"4957","DOI":"10.1016\/j.biomaterials.2012.03.041","article-title":"Role of sustained antigen release from nanoparticle vaccines in shaping the T cell memory phenotype","volume":"33","author":"Demento","year":"2012","journal-title":"Biomaterials"},{"key":"10.1016\/j.exppara.2016.09.008_bib12","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1128\/CMR.11.4.569","article-title":"Regulation and function of T-cell-mediated immunity during Toxoplasma gondii infection","volume":"11","author":"Denkers","year":"1998","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.exppara.2016.09.008_bib13","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.biomaterials.2015.01.056","article-title":"Porous nanoparticles as delivery system of complex antigens for an effective vaccine against acute and chronic Toxoplasma gondii infection","volume":"50","author":"Dimier-Poisson","year":"2015","journal-title":"Biomaterials"},{"key":"10.1016\/j.exppara.2016.09.008_bib14","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1111\/j.1550-7408.2008.00345.x","article-title":"The history of Toxoplasma gondii\u2014the first 100 years","volume":"55","author":"Dubey","year":"2008","journal-title":"J.\u00a0Eukaryot. Microbiol."},{"key":"10.1016\/j.exppara.2016.09.008_bib15","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1016\/j.vaccine.2010.11.002","article-title":"Evaluation of three recombinant multi-antigenic vaccines composed of surface and secretory antigens of Toxoplasma gondii in murine models of experimental toxoplasmosis","volume":"29","author":"Dziadek","year":"2011","journal-title":"Vaccine"},{"key":"10.1016\/j.exppara.2016.09.008_bib16","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.ijpharm.2011.05.049","article-title":"The mechanisms of drug release in poly (lactic-co-glycolic acid)-based drug delivery systems\u2014a review","volume":"415","author":"Fredenberg","year":"2011","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.exppara.2016.09.008_bib17","series-title":"Current Progress toward Vaccines against Toxoplasma Gondii","author":"Garcia","year":"2014"},{"key":"10.1016\/j.exppara.2016.09.008_bib18","doi-asserted-by":"crossref","first-page":"2446","DOI":"10.1128\/iai.58.8.2446-2451.1990","article-title":"Isotypic profiles of antibody responses to Toxoplasma gondii infection in rats and mice: kinetic study and characterization of target antigens of immunoglobulin A antibodies","volume":"58","author":"Godard","year":"1990","journal-title":"Infect. Immun."},{"key":"10.1016\/j.exppara.2016.09.008_bib19","first-page":"1","article-title":"Construction, expression and preliminary immunological evaluation of a DNA plasmid encoding the GRA2 protein of Toxoplasma gondii","volume":"9","author":"Golkar","year":"2005","journal-title":"Iran. Biomed. J."},{"key":"10.1016\/j.exppara.2016.09.008_bib20","doi-asserted-by":"crossref","first-page":"4301","DOI":"10.1016\/j.vaccine.2007.02.057","article-title":"Evaluation of protective effect of recombinant dense granule antigens GRA2 and GRA6 formulated in monophosphoryl lipid A (MPL) adjuvant against Toxoplasma chronic infection in mice","volume":"25","author":"Golkar","year":"2007","journal-title":"Vaccine"},{"key":"10.1016\/j.exppara.2016.09.008_bib21","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1016\/j.addr.2011.05.021","article-title":"Targeting dendritic cells with nano-particulate PLGA cancer vaccine formulations","volume":"63","author":"Hamdy","year":"2011","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.exppara.2016.09.008_bib22","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1073\/pnas.1011549108","article-title":"Critical coordination of innate immune defense against Toxoplasma gondii by dendritic cells responding via their Toll-like receptors","volume":"108","author":"Hou","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.exppara.2016.09.008_bib23","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1211\/0022357044418","article-title":"Development of a single-dose stabilized poly (d, l-lactic-co-glycolic acid) microspheres-based vaccine against hepatitis B","volume":"56","author":"Jaganathan","year":"2004","journal-title":"J.\u00a0Pharm. Pharmacol."},{"key":"10.1016\/j.exppara.2016.09.008_bib24","doi-asserted-by":"crossref","first-page":"1731","DOI":"10.1586\/erv.11.126","article-title":"The long-term potential of biodegradable poly(lactide-co-glycolide) microparticles as the next-generation vaccine adjuvant","volume":"10","author":"Jain","year":"2011","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.exppara.2016.09.008_bib25","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1590\/S0074-02762009000200019","article-title":"Vaccines against Toxoplasma gondii: challenges and opportunities","volume":"104","author":"Jongert","year":"2009","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"10.1016\/j.exppara.2016.09.008_bib26","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1590\/S0074-02762009000200019","article-title":"Vaccines against Toxoplasma gondii: challenges and opportunities","volume":"104","author":"Jongert","year":"2009","journal-title":"Memorias do Inst. Oswaldo Cruz"},{"key":"10.1016\/j.exppara.2016.09.008_bib27","doi-asserted-by":"crossref","first-page":"2629","DOI":"10.4049\/jimmunol.164.5.2629","article-title":"Decreased resistance of B cell-deficient mice to infection with Toxoplasma gondii despite unimpaired expression of IFN-\u03b3, TNF-\u03b1, and inducible nitric oxide synthase","volume":"164","author":"Kang","year":"2000","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.exppara.2016.09.008_bib28","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1016\/j.jconrel.2005.10.010","article-title":"Encapsulation of the immune potentiators MPL and RC529 in PLG microparticles enhances their potency","volume":"110","author":"Kazzaz","year":"2006","journal-title":"J.\u00a0Control Release"},{"key":"10.1016\/j.exppara.2016.09.008_bib29","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1080\/10611860801900462","article-title":"PLGA microspheres for the delivery of a novel subunit TB vaccine","volume":"16","author":"Kirby","year":"2008","journal-title":"J.\u00a0Drug Target."},{"key":"10.1016\/j.exppara.2016.09.008_bib30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.colsurfb.2009.09.001","article-title":"Biodegradable polymeric nanoparticles based drug delivery systems","volume":"75","author":"Kumari","year":"2010","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"10.1016\/j.exppara.2016.09.008_bib31","first-page":"369","article-title":"Preparation and in-vitro evaluation of controlled release PLGA microparticles containing triptoreline","volume":"9","author":"Mahboubian","year":"2010","journal-title":"Iran. J. Pharm. Res. IJPR"},{"key":"10.1016\/j.exppara.2016.09.008_bib32","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/S0927-7765(00)00148-X","article-title":"Pulsatile drug release from poly (lactide-co-glycolide) microspheres how does the composition the polymer matrices affect the time interval between the initial burst the pulsatile release drugs?","volume":"19","author":"Makino","year":"2000","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"10.1016\/j.exppara.2016.09.008_bib33","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.1002\/eji.200737984","article-title":"Nanoparticles target distinct dendritic cell populations according to their size","volume":"38","author":"Manolova","year":"2008","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/j.exppara.2016.09.008_bib34","first-page":"704","article-title":"Recombinant GRA4 or ROP2 protein combined with alum or the gra4 gene provides partial protection in chronic murine models of toxoplasmosis","volume":"11","author":"Martin","year":"2004","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"10.1016\/j.exppara.2016.09.008_bib35","doi-asserted-by":"crossref","first-page":"4489","DOI":"10.1016\/j.vaccine.2005.04.025","article-title":"Evaluation of protective effect of DNA vaccination with genes encoding antigens GRA4 and SAG1 associated with GM-CSF plasmid, against acute, chronical and congenital toxoplasmosis in mice","volume":"23","author":"M\u00e9v\u00e9lec","year":"2005","journal-title":"Vaccine"},{"key":"10.1016\/j.exppara.2016.09.008_bib36","doi-asserted-by":"crossref","first-page":"1965","DOI":"10.1016\/S0140-6736(04)16412-X","article-title":"Toxoplasmosis","volume":"363","author":"Montoya","year":"2004","journal-title":"Lancet"},{"key":"10.1016\/j.exppara.2016.09.008_bib37","doi-asserted-by":"crossref","first-page":"e31472","DOI":"10.1371\/journal.pone.0031472","article-title":"Antigen-displaying lipid-enveloped PLGA nanoparticles as delivery agents for a Plasmodium vivax malaria vaccine","volume":"7","author":"Moon","year":"2012","journal-title":"PloS One"},{"key":"10.1016\/j.exppara.2016.09.008_bib38","doi-asserted-by":"crossref","first-page":"S29","DOI":"10.3347\/kjp.2009.47.S.S29","article-title":"GRA proteins of Toxoplasma gondii: maintenance of host-parasite interactions across the parasitophorous vacuolar membrane","volume":"47","author":"Nam","year":"2009","journal-title":"Korean J. Parasitol."},{"key":"10.1016\/j.exppara.2016.09.008_bib39","doi-asserted-by":"crossref","first-page":"1675","DOI":"10.1128\/iai.65.5.1675-1682.1997","article-title":"Role of interleukin-10 in regulation of T-cell-dependent and T-cell-independent mechanisms of resistance to Toxoplasma gondii","volume":"65","author":"Neyer","year":"1997","journal-title":"Infect. Immun."},{"key":"10.1016\/j.exppara.2016.09.008_bib40","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/S0169-409X(98)00012-X","article-title":"Poly (lactide-co-glycolide) microparticles for the development of single-dose controlled-release vaccines","volume":"32","author":"O'Hagan","year":"1998","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.exppara.2016.09.008_bib41","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.ymeth.2006.05.017","article-title":"Microparticle-based technologies for vaccines","volume":"40","author":"O'Hagan","year":"2006","journal-title":"Methods"},{"key":"10.1016\/j.exppara.2016.09.008_bib42","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1016\/S0264-410X(98)00039-5","article-title":"Immunization with E. coli produced recombinant T. gondii SAG1 with alum as adjuvant protect mice against lethal infection with Toxoplasma gondii","volume":"16","author":"Petersen","year":"1998","journal-title":"Vaccine"},{"key":"10.1016\/j.exppara.2016.09.008_bib43","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1016\/j.pt.2011.03.009","article-title":"Innate responses to Toxoplasma gondii in mice and humans","volume":"27","author":"Pifer","year":"2011","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.exppara.2016.09.008_bib44","doi-asserted-by":"crossref","first-page":"4445","DOI":"10.1016\/S0264-410X(01)00192-X","article-title":"Biodegradable PLGA microspheres as a delivery system for malaria synthetic peptide SPf66","volume":"19","author":"Rosas","year":"2001","journal-title":"Vaccine"},{"key":"10.1016\/j.exppara.2016.09.008_bib45","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.ijpharm.2011.01.045","article-title":"Comparison of humoral and cell-mediated immune responses to cationic PLGA microspheres containing recombinant hepatitis B antigen","volume":"408","author":"Saini","year":"2011","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.exppara.2016.09.008_bib46","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1016\/j.vaccine.2011.11.057","article-title":"Combination of immune stimulating adjuvants with poly(lactide-co-glycolide) microspheres enhances the immune response of vaccines","volume":"30","author":"Salvador","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.exppara.2016.09.008_bib47","first-page":"2115","article-title":"Towards development of novel immunization strategies against leishmaniasis using PLGA nanoparticles loaded with kinetoplastid membrane protein-11","volume":"7","author":"Santos","year":"2012","journal-title":"Int. J. Nanomedicine"},{"key":"10.1016\/j.exppara.2016.09.008_bib48","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1016\/j.nano.2013.04.003","article-title":"PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania","volume":"9","author":"Santos","year":"2013","journal-title":"Nanomedicine Nanotechnol. Biol. Med."},{"key":"10.1016\/j.exppara.2016.09.008_bib49","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1128\/IAI.68.3.1026-1033.2000","article-title":"B\u00a0cells are essential for vaccination-induced resistance to virulent Toxoplasma gondii","volume":"68","author":"Sayles","year":"2000","journal-title":"Infect. Immun."},{"key":"10.1016\/j.exppara.2016.09.008_bib50","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/S0923-2494(05)80095-1","article-title":"Macrophage interactions in toxoplasmosis","volume":"144","author":"Sibley","year":"1993","journal-title":"Res. Immunol."},{"key":"10.1016\/j.exppara.2016.09.008_bib51","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1002\/jps.10538","article-title":"Anionic microparticles are a potent delivery system for recombinant antigens from Neisseria meningitidis serotype B","volume":"93","author":"Singh","year":"2004","journal-title":"J.\u00a0Pharm. Sci."},{"key":"10.1016\/j.exppara.2016.09.008_bib52","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.jconrel.2009.08.023","article-title":"Strong antibody responses induced by protein antigens conjugated onto the surface of lecithin-based nanoparticles","volume":"141","author":"Sloat","year":"2010","journal-title":"J.\u00a0Control. Release"},{"key":"10.1016\/j.exppara.2016.09.008_bib53","doi-asserted-by":"crossref","first-page":"3090","DOI":"10.1128\/IAI.01722-14","article-title":"Complex immune cell interplay in the gamma interferon response during Toxoplasma gondii infection","volume":"82","author":"Sturge","year":"2014","journal-title":"Infect. Immun."},{"key":"10.1016\/j.exppara.2016.09.008_bib54","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1126\/science.3128869","article-title":"Interferon-gamma: the major mediator of resistance against Toxoplasma gondii","volume":"240","author":"Suzuki","year":"1988","journal-title":"Science"},{"key":"10.1016\/j.exppara.2016.09.008_bib55","doi-asserted-by":"crossref","first-page":"5375","DOI":"10.4049\/jimmunol.164.10.5375","article-title":"IL-10 is required for prevention of necrosis in the small intestine and mortality in both genetically resistant BALB\/c and susceptible C57BL\/6 mice following peroral infection with Toxoplasma gondii","volume":"164","author":"Suzuki","year":"2000","journal-title":"J.\u00a0Immunol."},{"key":"10.1016\/j.exppara.2016.09.008_bib56","doi-asserted-by":"crossref","first-page":"e31","DOI":"10.1017\/S1462399411002018","article-title":"Interferon-gamma-and perforin-mediated immune responses for resistance against Toxoplasma gondii in the brain","volume":"13","author":"Suzuki","year":"2011","journal-title":"Expert Rev. Mol. Med."},{"key":"10.1016\/j.exppara.2016.09.008_bib57","first-page":"639","article-title":"Immunization against leishmaniasis by PLGA nanospheres loaded with an experimental Autoclaved Leishmania major (ALM) and Quillajasaponins","volume":"27","author":"Tafaghodi","year":"2010","journal-title":"Trop. Biomed."},{"key":"10.1016\/j.exppara.2016.09.008_bib58","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1016\/S0020-7519(00)00124-7","article-title":"Toxoplasma gondii: from animals to humans","volume":"30","author":"Tenter","year":"2000","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.exppara.2016.09.008_bib59","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1021\/mp100255c","article-title":"Aerosolized PLA and PLGA nanoparticles enhance humoral, mucosal and cytokine responses to hepatitis B vaccine","volume":"8","author":"Thomas","year":"2011","journal-title":"Mol. Pharm."},{"key":"10.1016\/j.exppara.2016.09.008_bib60","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1023\/A:1026498209874","article-title":"Protein instability in poly (lactic-co-glycolic acid) microparticles","volume":"17","author":"van de Weert","year":"2000","journal-title":"Pharm. Res."},{"key":"10.1016\/j.exppara.2016.09.008_bib61","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.addr.2014.05.011","article-title":"Combatting infectious diseases; nanotechnology as a platform for rational vaccine design","volume":"74","author":"van Riet","year":"2014","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.exppara.2016.09.008_bib62","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1017\/S0031182001008460","article-title":"Anti-SAG1 peptide antibodies inhibit the penetration of Toxoplasma gondii tachyzoites into enterocyte cell lines","volume":"123","author":"Velge-Roussel","year":"2001","journal-title":"Parasitology"},{"key":"10.1016\/j.exppara.2016.09.008_bib63","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/j.addr.2004.09.007","article-title":"PLGA microspheres for improved antigen delivery to dendritic cells as cellular vaccines","volume":"57","author":"Waeckerle-Men","year":"2005","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.exppara.2016.09.008_bib64","doi-asserted-by":"crossref","first-page":"e0126334","DOI":"10.1371\/journal.pone.0126334","article-title":"Prime-boost vaccination with Toxoplasma lysate antigen, but not with a mixture of recombinant protein antigens, leads to reduction of brain cyst formation in BALB\/c mice","volume":"10","author":"Wagner","year":"2015","journal-title":"PloS One"},{"key":"10.1016\/j.exppara.2016.09.008_bib65","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1186\/1756-3305-7-180","article-title":"Research progress on surface antigen 1 (SAG1) of Toxoplasma gondii","volume":"7","author":"Wang","year":"2014","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.exppara.2016.09.008_bib66","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1208\/s12248-009-9089-0","article-title":"Screening for potential adjuvants administered by the pulmonary route for tuberculosis vaccines","volume":"11","author":"Wang","year":"2009","journal-title":"AAPS J."},{"key":"10.1016\/j.exppara.2016.09.008_bib67","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/j.ijpharm.2008.04.042","article-title":"Principles of encapsulating hydrophobic drugs in PLA\/PLGA microparticles","volume":"364","author":"Wischke","year":"2008","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.exppara.2016.09.008_bib68","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.exppara.2011.10.006","article-title":"Separation and purification of Toxoplasma gondii tachyzoites from in\u00a0vitro and in\u00a0vivo culture systems","volume":"130","author":"Wu","year":"2012","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.exppara.2016.09.008_bib69","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1038\/nri3598","article-title":"Innate immunity to Toxoplasma gondii infection","volume":"14","author":"Yarovinsky","year":"2014","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.exppara.2016.09.008_bib70","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1046\/j.1365-3024.1999.00229.x","article-title":"Protective immunity in the rat model of congenital toxoplasmosis and the potential of excreted-secreted antigens as vaccine components","volume":"21","author":"Zenner","year":"1999","journal-title":"Parasite Immunol."},{"key":"10.1016\/j.exppara.2016.09.008_bib71","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1586\/14760584.2013.844652","article-title":"Vaccines against Toxoplasma gondii: new developments and perspectives","volume":"12","author":"Zhang","year":"2013","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.exppara.2016.09.008_bib72","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1007\/s00436-006-0341-6","article-title":"Toxoplasma gondii: expression and characterization of a recombinant protein containing SAG1 and GRA2 in Pichia pastoris","volume":"100","author":"Zhou","year":"2007","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.exppara.2016.09.008_bib73","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.jconrel.2007.05.034","article-title":"Effect of acidic pH on PLGA microsphere degradation and release","volume":"122","author":"Zolnik","year":"2007","journal-title":"J.\u00a0Control. Release"}],"container-title":["Experimental Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489416301904?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489416301904?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T06:24:10Z","timestamp":1758003850000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014489416301904"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11]]},"references-count":73,"alternative-id":["S0014489416301904"],"URL":"https:\/\/doi.org\/10.1016\/j.exppara.2016.09.008","relation":{},"ISSN":["0014-4894"],"issn-type":[{"value":"0014-4894","type":"print"}],"subject":[],"published":{"date-parts":[[2016,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Synergistic effect of rSAG1 and rGRA2 antigens formulated in PLGA microspheres in eliciting immune protection against Toxoplasama gondii","name":"articletitle","label":"Article Title"},{"value":"Experimental Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exppara.2016.09.008","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}