{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T01:36:22Z","timestamp":1772501782724,"version":"3.50.1"},"reference-count":30,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T00:00:00Z","timestamp":1504224000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Robert Koch Institute"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Parasitology"],"published-print":{"date-parts":[[2017,9]]},"DOI":"10.1016\/j.exppara.2016.12.015","type":"journal-article","created":{"date-parts":[[2016,12,21]],"date-time":"2016-12-21T06:16:23Z","timestamp":1482300983000},"page":"19-26","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Toxoplasma gondii plaque assays revisited: Improvements for ultrastructural and quantitative evaluation of lytic parasite growth"],"prefix":"10.1016","volume":"180","author":[{"given":"Christoph-Martin","family":"Ufermann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florian","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nora","family":"Frohnecke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Laue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2271-3699","authenticated-orcid":false,"given":"Frank","family":"Seeber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.exppara.2016.12.015_bib1","doi-asserted-by":"crossref","first-page":"e2973","DOI":"10.1371\/journal.pntd.0002973","article-title":"Auranofin is highly efficacious against Toxoplasma gondii in\u00a0vitro and in an in\u00a0vivo experimental model of acute toxoplasmosis","volume":"8","author":"Andrade","year":"2014","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.exppara.2016.12.015_bib2","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1128\/EC.00229-14","article-title":"Genetic mapping reveals that sinefungin resistance in Toxoplasma gondii is controlled by a putative amino acid transporter locus that can be used as a negative selectable marker","volume":"14","author":"Behnke","year":"2015","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.exppara.2016.12.015_bib3","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1017\/S0031182004006821","article-title":"Host serum modifies the drug susceptibility of Babesia bovis in\u00a0vitro","volume":"130","author":"Bork","year":"2005","journal-title":"Parasitology"},{"key":"10.1016\/j.exppara.2016.12.015_bib4","doi-asserted-by":"crossref","first-page":"53","DOI":"10.14573\/altex.2010.1.53","article-title":"Serum-free cell culture: the serum-free media interactive online database","volume":"27","author":"Brunner","year":"2010","journal-title":"ALTEX"},{"key":"10.1016\/j.exppara.2016.12.015_bib5","doi-asserted-by":"crossref","first-page":"431","DOI":"10.3181\/00379727-102-25275","article-title":"Plaque assay of Toxoplasma on monolayers of chick embryo fibroblasts","volume":"102","author":"Chaparas","year":"1959","journal-title":"Proc. Soc. Exp. Biol. Med."},{"key":"10.1016\/j.exppara.2016.12.015_bib6","doi-asserted-by":"crossref","first-page":"3214","DOI":"10.1038\/sj.emboj.7601189","article-title":"Toxoplasma gondii scavenges host-derived lipoic acid despite its de novo synthesis in the apicoplast","volume":"25","author":"Crawford","year":"2006","journal-title":"EMBO J."},{"key":"10.1016\/j.exppara.2016.12.015_bib7","doi-asserted-by":"crossref","first-page":"5749","DOI":"10.1073\/pnas.92.12.5749","article-title":"Insertional mutagenesis and marker rescue in a protozoan parasite: cloning of the uracil phosphoribosyltransferase locus from Toxoplasma gondii","volume":"92","author":"Donald","year":"1995","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.exppara.2016.12.015_bib8","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1083\/jcb.115.2.423","article-title":"Fiber type- and position-dependent expression of a myosin light chain-CAT transgene detected with a novel histochemical stain for CAT","volume":"115","author":"Donoghue","year":"1991","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.exppara.2016.12.015_bib9","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1016\/j.pt.2009.06.006","article-title":"Reporter genes facilitating discovery of drugs targeting protozoan parasites","volume":"25","author":"Dube","year":"2009","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.exppara.2016.12.015_bib10","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1016\/j.ijpara.2009.02.009","article-title":"The role played by electron microscopy in advancing our understanding of Toxoplasma gondii and other apicomplexans","volume":"39","author":"Dubremetz","year":"2009","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.exppara.2016.12.015_bib11","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1084\/jem.99.2.167","article-title":"Plaque formation and isolation of pure lines with poliomyelitis viruses","volume":"99","author":"Dulbecco","year":"1954","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.exppara.2016.12.015_bib12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/JB.98.1.1-3.1969","article-title":"Plaquing of Toxoplasma gondii in secondary cultures of chick embryo fibroblasts","volume":"98","author":"Foley","year":"1969","journal-title":"J.\u00a0Bacteriol."},{"key":"10.1016\/j.exppara.2016.12.015_bib13","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1128\/EC.00357-08","article-title":"Efficient gene replacements in Toxoplasma gondii strains deficient for nonhomologous end joining","volume":"8","author":"Fox","year":"2009","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.exppara.2016.12.015_bib14","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/BF00495720","article-title":"Tetrazoliummethoden zum histochemischen Hydrolasennachweis","volume":"58","author":"Gossrau","year":"1978","journal-title":"Histochemistry"},{"key":"10.1016\/j.exppara.2016.12.015_bib15","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1007\/s10616-013-9633-8","article-title":"A\u00a0plea to reduce or replace fetal bovine serum in cell culture media","volume":"65","author":"Gstraunthaler","year":"2013","journal-title":"Cytotechnology"},{"key":"10.1016\/j.exppara.2016.12.015_bib16","series-title":"Toxoplasma gondii: the Model Apicomplexan - Perspectives and Methods","first-page":"578","article-title":"Genetic manipulation of Toxoplasma gondii","author":"Jacot","year":"2014"},{"key":"10.1016\/j.exppara.2016.12.015_bib17","first-page":"507","article-title":"Predicting plasma protein binding of drugs\u2013revisited","volume":"7","author":"Kratochwil","year":"2004","journal-title":"Curr. Opin. Drug Discov. Devel"},{"key":"10.1016\/j.exppara.2016.12.015_bib18","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/B978-0-12-801075-4.00005-7","article-title":"A\u00a0simple procedure to analyze positions of interest in infectious cell cultures by correlative light and electron microscopy","volume":"124","author":"Madela","year":"2014","journal-title":"Methods Cell Biol."},{"key":"10.1016\/j.exppara.2016.12.015_bib19","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1186\/1743-422X-3-63","article-title":"New low-viscosity overlay medium for viral plaque assays","volume":"3","author":"Matrosovich","year":"2006","journal-title":"Virol. J."},{"key":"10.1016\/j.exppara.2016.12.015_bib20","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1186\/1756-3305-6-334","article-title":"Dark side illuminated: imaging of Toxoplasma gondii through the decades","volume":"6","author":"McGovern","year":"2013","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.exppara.2016.12.015_bib21","doi-asserted-by":"crossref","first-page":"214","DOI":"10.4269\/ajtmh.1994.51.214","article-title":"Determination of fifty percent inhibitory concentrations (IC50) of antimalarial drugs against Plasmodium falciparum parasites in a serum-free medium","volume":"51","author":"Ofulla","year":"1994","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.exppara.2016.12.015_bib22","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1111\/j.1550-7408.1977.tb04774.x","article-title":"Specific labelling of intracellular Toxoplasma gondii with uracil","volume":"24","author":"Pfefferkorn","year":"1977","journal-title":"J.\u00a0Protozool."},{"key":"10.1016\/j.exppara.2016.12.015_bib23","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/0014-4894(76)90040-0","article-title":"Toxoplasma gondii: isolation and preliminary characterization of temperature-sensitive mutants","volume":"39","author":"Pfefferkorn","year":"1976","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.exppara.2016.12.015_bib24","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0091-679X(08)61845-2","article-title":"Molecular tools for genetic dissection of the protozoan parasite Toxoplasma gondii","volume":"45","author":"Roos","year":"1994","journal-title":"Methods Cell Biol."},{"key":"10.1016\/j.exppara.2016.12.015_bib25","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Meth"},{"key":"10.1016\/j.exppara.2016.12.015_bib26","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Meth"},{"key":"10.1016\/j.exppara.2016.12.015_bib27","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/0378-1119(95)00786-5","article-title":"Escherichia coli beta-galactosidase as an in\u00a0vitro and in\u00a0vivo reporter enzyme and stable transfection marker in the intracellular protozoan parasite Toxoplasma gondii","volume":"169","author":"Seeber","year":"1996","journal-title":"Gene"},{"key":"10.1016\/j.exppara.2016.12.015_bib28","doi-asserted-by":"crossref","first-page":"e01114","DOI":"10.1128\/mBio.01114-14","article-title":"Efficient gene disruption in diverse strains of Toxoplasma gondii using CRISPR\/CAS9","volume":"5","author":"Shen","year":"2014","journal-title":"mBio"},{"key":"10.1016\/j.exppara.2016.12.015_bib29","doi-asserted-by":"crossref","first-page":"e100450","DOI":"10.1371\/journal.pone.0100450","article-title":"Efficient genome engineering of Toxoplasma gondii using CRISPR\/Cas9","volume":"9","author":"Sidik","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.exppara.2016.12.015_bib30","doi-asserted-by":"crossref","first-page":"e0157873","DOI":"10.1371\/journal.pone.0157873","article-title":"Development and application of a simple plaque assay for the human malaria parasite Plasmodium falciparum","volume":"11","author":"Thomas","year":"2016","journal-title":"PLoS One"}],"container-title":["Experimental Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489416303939?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489416303939?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,4,3]],"date-time":"2021-04-03T15:06:10Z","timestamp":1617462370000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014489416303939"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9]]},"references-count":30,"alternative-id":["S0014489416303939"],"URL":"https:\/\/doi.org\/10.1016\/j.exppara.2016.12.015","relation":{},"ISSN":["0014-4894"],"issn-type":[{"value":"0014-4894","type":"print"}],"subject":[],"published":{"date-parts":[[2017,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Toxoplasma gondii plaque assays revisited: Improvements for ultrastructural and quantitative evaluation of lytic parasite growth","name":"articletitle","label":"Article Title"},{"value":"Experimental Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exppara.2016.12.015","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}