{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T15:06:30Z","timestamp":1790262390409,"version":"4.1.0"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T00:00:00Z","timestamp":1512086400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100005851","name":"Shahid Beheshti University of Medical Sciences","doi-asserted-by":"publisher","award":["6231"],"award-info":[{"award-number":["6231"]}],"id":[{"id":"10.13039\/501100005851","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015528","name":"Universidad de La Laguna","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015528","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Parasitology"],"published-print":{"date-parts":[[2017,12]]},"DOI":"10.1016\/j.exppara.2017.09.016","type":"journal-article","created":{"date-parts":[[2017,9,13]],"date-time":"2017-09-13T05:01:01Z","timestamp":1505278861000},"page":"240-244","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":47,"special_numbering":"C","title":["Molecular identification of waterborne free living amoebae (Acanthamoeba, Naegleria and Vermamoeba) isolated from municipal drinking water and environmental sources, Semnan province, north half of Iran"],"prefix":"10.1016","volume":"183","author":[{"given":"Ehsan","family":"Javanmard","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Niyyati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob","family":"Lorenzo-Morales","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zohreh","family":"Lasjerdi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4596-3789","authenticated-orcid":false,"given":"Hamed","family":"Behniafar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamed","family":"Mirjalali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.exppara.2017.09.016_bib1","first-page":"481","article-title":"First report of Hartmannella keratitis in a cosmetic soft contact lens wearer in Iran","volume":"8","author":"Abedkhojasteh","year":"2013","journal-title":"Iran. J. Parasitol."},{"key":"10.1016\/j.exppara.2017.09.016_bib2","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.ejop.2014.10.001","article-title":"Identifying differentially expressed genes in trophozoites and cysts of Acanthamoeba T4 genotype: implications for developing new treatments for Acanthamoeba keratitis","volume":"51","author":"Abedkhojasteh","year":"2015","journal-title":"Eur. J. protistology"},{"key":"10.1016\/j.exppara.2017.09.016_bib3","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1128\/JCM.41.1.453-455.2003","article-title":"Identification of Balamuthia mandrillaris by PCR assay using the mitochondrial 16S rRNA gene as a target","volume":"41","author":"Booton","year":"2003","journal-title":"J.\u00a0Clin. Microbiol."},{"key":"10.1016\/j.exppara.2017.09.016_bib4","doi-asserted-by":"crossref","first-page":"S2","DOI":"10.1016\/j.exppara.2014.07.011","article-title":"What do we know by now about the genus Naegleria?","volume":"145","author":"De Jonckheere","year":"2014","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.exppara.2017.09.016_bib5","doi-asserted-by":"crossref","first-page":"3147","DOI":"10.1007\/s00436-016-5072-8","article-title":"Isolation and genotyping of Acanthamoeba strains (T4, T9, and T11) from amoebic keratitis patients in Iran","volume":"115","author":"Hajialilo","year":"2016","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.exppara.2017.09.016_bib6","first-page":"541","article-title":"Pathogenic free-living amoebae isolated from contact lenses of keratitis patients","volume":"10","author":"Hajialilo","year":"2015","journal-title":"Iran. J. Parasitol."},{"key":"10.1016\/j.exppara.2017.09.016_bib7","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1111\/j.1574-6976.2006.00023.x","article-title":"Acanthamoeba: biology and increasing importance in human health","volume":"30","author":"Khan","year":"2006","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.exppara.2017.09.016_bib8","series-title":"Acanthamoeba: Biology and Pathogenesis","author":"Khan","year":"2009"},{"key":"10.1016\/j.exppara.2017.09.016_bib9","doi-asserted-by":"crossref","DOI":"10.1007\/s00436-015-4549-1","article-title":"The role of domestic tap water on Acanthamoeba keratitis in non-contact lens wearers and validation of laboratory methods","volume":"114","author":"Koltas","year":"2015","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.exppara.2017.09.016_bib10","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.diagmicrobio.2015.04.007","article-title":"Novel culture medium for the axenic growth of Balamuthia mandrillaris","volume":"82","author":"Lares-Jim\u00e9nez","year":"2015","journal-title":"Diagnostic Microbiol. Infect. Dis."},{"key":"10.1016\/j.exppara.2017.09.016_bib11","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1007\/s00436-011-2288-5","article-title":"Potentially pathogenic free-living amoebae isolated from hospital wards with immunodeficient patients in Tehran, Iran","volume":"109","author":"Lasjerdi","year":"2011","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.exppara.2017.09.016_bib12","doi-asserted-by":"crossref","first-page":"2456","DOI":"10.1017\/S095026881600073X","article-title":"Presence of Balamuthia mandrillaris in hot springs from Mazandaran province, northern Iran","volume":"144","author":"Latifi","year":"2016","journal-title":"Epidemiol. Infect."},{"key":"10.1016\/j.exppara.2017.09.016_bib13","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1515\/ap-2017-0012","article-title":"Occurrence of Naegleria species in therapeutic geothermal water sources, Northern Iran","volume":"62","author":"Latifi","year":"2017","journal-title":"Acta Parasitol."},{"key":"10.1016\/j.exppara.2017.09.016_bib14","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1097\/ICL.0b013e31827a79ee","article-title":"Acanthamoeba keratitis associated with tap water use during contact lens cleaning: manufacturer guidelines need to change","volume":"39","author":"Legarreta","year":"2013","journal-title":"Eye contact lens"},{"key":"10.1016\/j.exppara.2017.09.016_bib15","doi-asserted-by":"crossref","DOI":"10.1051\/parasite\/2015010","article-title":"An update on Acanthamoeba keratitis: diagnosis, pathogenesis and treatment","volume":"22","author":"Lorenzo-Morales","year":"2015","journal-title":"Parasite"},{"key":"10.1016\/j.exppara.2017.09.016_bib16","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.pt.2013.01.006","article-title":"Acanthamoeba keratitis: an emerging disease gathering importance worldwide?","volume":"29","author":"Lorenzo-Morales","year":"2013","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.exppara.2017.09.016_bib17","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1007\/s00436-007-0754-x","article-title":"Early diagnosis of amoebic keratitis due to a mixed infection with Acanthamoeba and Hartmannella","volume":"102","author":"Lorenzo-Morales","year":"2007","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.exppara.2017.09.016_bib18","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1007\/s00436-005-1301-2","article-title":"Isolation and identification of pathogenic Acanthamoeba strains in Tenerife, Canary Islands, Spain from water sources","volume":"95","author":"Lorenzo-Morales","year":"2005","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.exppara.2017.09.016_bib19","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1099\/jmm.0.45970-0","article-title":"Acanthamoeba genotype T4 from the UK and Iran and isolation of the T2 genotype from clinical isolates","volume":"54","author":"Maghsood","year":"2005","journal-title":"J.\u00a0Med. Microbiol."},{"key":"10.1016\/j.exppara.2017.09.016_bib20","doi-asserted-by":"crossref","first-page":"e0169448","DOI":"10.1371\/journal.pone.0169448","article-title":"Pathogenic waterborne free-living amoebae: an update from selected Southeast Asian countries","volume":"12","author":"Majid","year":"2017","journal-title":"PloS one"},{"key":"10.1016\/j.exppara.2017.09.016_bib21","doi-asserted-by":"crossref","first-page":"5864","DOI":"10.1128\/AEM.69.10.5864-5869.2003","article-title":"Identification of Naegleria fowleri in domestic water sources by nested PCR","volume":"69","author":"Marciano-Cabral","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.exppara.2017.09.016_bib22","doi-asserted-by":"crossref","DOI":"10.1155\/2012\/782854","article-title":"Primary amoebic meningoencephalitis in an Iranian infant","volume":"2012","author":"Movahedi","year":"2012","journal-title":"Case Rep. Med."},{"key":"10.1016\/j.exppara.2017.09.016_bib23","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1007\/s00436-012-2906-x","article-title":"Molecular identification of Hartmannella vermiformis and Vannella persistens from man-made recreational water environments, Tehran","volume":"111","author":"Nazar","year":"2012","journal-title":"Iran. Parasitol. Res."},{"key":"10.1016\/j.exppara.2017.09.016_bib24","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1007\/s00436-015-4770-y","article-title":"Isolation of Balamuthia mandrillaris from soil samples in North-Western Iran","volume":"115","author":"Niyyati","year":"2016","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.exppara.2017.09.016_bib25","doi-asserted-by":"crossref","first-page":"140","DOI":"10.2166\/wh.2011.068","article-title":"Screening of recreational areas of rivers for potentially pathogenic free-living amoebae in the suburbs of Tehran, Iran","volume":"10","author":"Niyyati","year":"2012","journal-title":"J.\u00a0water health"},{"key":"10.1016\/j.exppara.2017.09.016_bib26","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1007\/s00436-009-1592-9","article-title":"Isolation of Balamuthia mandrillaris from urban dust, free of known infectious involvement","volume":"106","author":"Niyyati","year":"2009","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.exppara.2017.09.016_bib27","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.exppara.2009.10.009","article-title":"First report of a mixed infection due to Acanthamoeba genotype T3 and Vahlkampfia in a cosmetic soft contact lens wearer in Iran","volume":"126","author":"Niyyati","year":"2010","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.exppara.2017.09.016_bib28","first-page":"157","article-title":"Current status of Acanthamoeba in Iran: a narrative review article","volume":"10","author":"Niyyati","year":"2015","journal-title":"Iran. J. Parasitol."},{"issue":"Suppl 1","key":"10.1016\/j.exppara.2017.09.016_bib35","first-page":"1","article-title":"Detection and molecular characterization of potentially pathogenic free-living amoebae from water sources in Kish Island, Southern Iran","volume":"8","author":"Niyyati","year":"2015","journal-title":"Microbiol. Insights"},{"key":"10.1016\/j.exppara.2017.09.016_bib29","doi-asserted-by":"crossref","first-page":"2061","DOI":"10.1128\/AEM.68.4.2061-2065.2002","article-title":"Use of multiplex PCR and PCR restriction enzyme analysis for detection and exploration of the variability in the free-living amoeba Naegleria in the environment","volume":"68","author":"P\u00e9landakis","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.exppara.2017.09.016_bib30","doi-asserted-by":"crossref","first-page":"1903","DOI":"10.1128\/JCM.39.5.1903-1911.2001","article-title":"Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of acanthamoebae from humans with keratitis and from sewage sludge","volume":"39","author":"Schroeder","year":"2001","journal-title":"J.\u00a0Clin. Microbiol."},{"key":"10.1016\/j.exppara.2017.09.016_bib31","doi-asserted-by":"crossref","first-page":"99","DOI":"10.2166\/wh.2007.014","article-title":"Prevalence of Acanthamoeba and other naked amoebae in South Florida domestic water","volume":"6","author":"Shoff","year":"2008","journal-title":"J.\u00a0Water Health"},{"key":"10.1016\/j.exppara.2017.09.016_bib32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-695X.2007.00232.x","article-title":"Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea","volume":"50","author":"Visvesvara","year":"2007","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"10.1016\/j.exppara.2017.09.016_bib33","doi-asserted-by":"crossref","first-page":"371","DOI":"10.2166\/wh.2013.112","article-title":"Status of free-living amoebae (Acanthamoeba spp., Naegleria fowleri, Balamuthia mandrillaris) in drinking water supplies in Karachi, Pakistan","volume":"11","author":"Yousuf","year":"2013","journal-title":"J.\u00a0water health"},{"key":"10.1016\/j.exppara.2017.09.016_bib34","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1099\/jmm.0.016667-0","article-title":"Genotyping of Acanthamoeba isolates and clinical characteristics of patients with Acanthamoeba keratitis in China","volume":"59","author":"Zhao","year":"2010","journal-title":"J.\u00a0Med. Microbiol."}],"container-title":["Experimental Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489417304198?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489417304198?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T04:42:45Z","timestamp":1668573765000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014489417304198"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12]]},"references-count":35,"alternative-id":["S0014489417304198"],"URL":"https:\/\/doi.org\/10.1016\/j.exppara.2017.09.016","relation":{},"ISSN":["0014-4894"],"issn-type":[{"value":"0014-4894","type":"print"}],"subject":[],"published":{"date-parts":[[2017,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular identification of waterborne free living amoebae (Acanthamoeba, Naegleria and Vermamoeba) isolated from municipal drinking water and environmental sources, Semnan province, north half of Iran","name":"articletitle","label":"Article Title"},{"value":"Experimental Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exppara.2017.09.016","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}