{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T22:14:38Z","timestamp":1784758478142,"version":"3.55.0"},"reference-count":37,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,8,1]],"date-time":"2019-08-01T00:00:00Z","timestamp":1564617600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Rede Mineira de Biomol\u00e9culas","award":["CCB-RED00012-14"],"award-info":[{"award-number":["CCB-RED00012-14"]}]},{"DOI":"10.13039\/501100007375","name":"Pr\u00f3-Reitoria de Pesquisa, Universidade Federal de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007375","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Parasitology"],"published-print":{"date-parts":[[2019,8]]},"DOI":"10.1016\/j.exppara.2019.05.006","type":"journal-article","created":{"date-parts":[[2019,5,28]],"date-time":"2019-05-28T08:17:49Z","timestamp":1559031469000},"page":"23-29","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"special_numbering":"C","title":["Development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of Leishmania amazonensis in skin samples"],"prefix":"10.1016","volume":"203","author":[{"given":"Jordanna Luiza de Lima","family":"Celeste","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta Lima","family":"Caldeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone da Fonseca","family":"Pires","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine Dias","family":"Silveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo Pedro","family":"Soares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9lida Monteiro","family":"de Andrade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.exppara.2019.05.006_bib1","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.actatropica.2016.06.009","article-title":"Optimization of loop-mediated isothermal amplification (LAMP) assays for the detection of Leishmania DNA in human blood samples","volume":"162","author":"Abbasi","year":"2016","journal-title":"Acta Trop."},{"key":"10.1016\/j.exppara.2019.05.006_bib2","doi-asserted-by":"crossref","first-page":"591","DOI":"10.4269\/ajtmh.2010.09-0369","article-title":"Development of a reverse transcriptase loop-mediated isothermal amplification (LAMP) assay for the sensitive detection of Leishmania parasites in clinical samples","volume":"82","author":"Adams","year":"2010","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.exppara.2019.05.006_bib3","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1016\/j.resmic.2011.04.015","article-title":"Mitochondrial RNA processing in trypanosomes","volume":"162","author":"Aphasizhev","year":"2011","journal-title":"Res. Microbiol."},{"key":"10.1016\/j.exppara.2019.05.006_bib4","doi-asserted-by":"crossref","first-page":"732","DOI":"10.4269\/ajtmh.1986.35.732","article-title":"Isolation of Leishmania mexicana amazonensis from the bone marrow in a case of American visceral leishmaniasis","volume":"35","author":"Barral","year":"1986","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.exppara.2019.05.006_bib5","doi-asserted-by":"crossref","first-page":"536","DOI":"10.4269\/ajtmh.1991.44.536","article-title":"Leishmaniasis in Bahia, Brazil: evidence that Leishmania amazonensis produces a wide spectrum of clinical disease","volume":"44","author":"Barral","year":"1991","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.exppara.2019.05.006_bib6","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1128\/CDLI.8.4.828-831.2001","article-title":"Real-time PCR as a new tool for quantifying Leishmania infantum in liver in infected mice","volume":"8","author":"Bretagne","year":"2001","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"10.1016\/j.exppara.2019.05.006_bib7","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1016\/S0020-7519(01)00265-X","article-title":"Detection of malaria liver-stages in mice infected through the bite of a single Anopheles mosquito using a highly sensitive real-time PCR","volume":"31","author":"Bruna-Romero","year":"2001","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.exppara.2019.05.006_bib8","article-title":"Experimental mixed infection of Leishmania (Leishmania) amazonensis and Leishmania (L.) infantum in hamsters (Mesocricetus auratus)","author":"Celeste","year":"2017","journal-title":"Parasitology"},{"key":"10.1016\/j.exppara.2019.05.006_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetpar.2013.07.038","article-title":"Development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of Leishmania infantum in canine leishmaniasis based on cysteine protease B genes","author":"Chaouch","year":"2013","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.exppara.2019.05.006_bib10","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1007\/s00436-017-5726-1","article-title":"New primers for the detection Leishmania species by multiplex polymerase chain reaction","volume":"117","author":"Conter","year":"2018","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.exppara.2019.05.006_bib11","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.vetpar.2010.11.014","article-title":"Eco-epidemiology of visceral leishmaniasis in the urban area of Paracatu, state of Minas Gerais, Brazil","volume":"176","author":"Dias","year":"2011","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.exppara.2019.05.006_bib12","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1590\/S0037-86822006000100008","article-title":"Occurrence of American tegumentary leishmaniasis in the mato grosso do sul state associated to the infection for Leishmania (Leishmania) amazonensis","volume":"39","author":"Dorval","year":"2006","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"10.1016\/j.exppara.2019.05.006_bib13","doi-asserted-by":"crossref","DOI":"10.1186\/s13071-015-0991-2","article-title":"Development of a LAMP assay for detection of Leishmania infantum infection in dogs using conjunctival swab samples","author":"Gao","year":"2015","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.exppara.2019.05.006_bib14","first-page":"50","article-title":"Development and assessment of loop-mediated isothermal amplification (LAMP) assay for the diagnosis of human visceral leishmaniasis in Iran","volume":"9","author":"Ghasemian","year":"2014","journal-title":"Irian. J. Parasitol."},{"key":"10.1016\/j.exppara.2019.05.006_bib15","doi-asserted-by":"crossref","first-page":"3265","DOI":"10.5433\/1679-0359.2012v33Supl2p3265","article-title":"Leishmania amazonensis em c\u00e3o com quadro cl\u00ednico de leishmaniose visceral no Estado do Paran\u00e1, Brasil \u2013 relato de caso","volume":"33","author":"Hoffmann","year":"2012","journal-title":"Semina Ci\u00eancias Agr\u00e1rias"},{"key":"10.1016\/j.exppara.2019.05.006_bib16","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1186\/s13071-018-2836-2","article-title":"Evaluation of fluorimetry and direct visualization to interpret results of a loop mediated isothermal amplification kit to detect Leishmania DNA","volume":"11","author":"Ibarra-Meneses","year":"2018","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.exppara.2019.05.006_bib17","article-title":"Non-invasive diagnosis of cutaneous leishmaniasis by the direct boil loop-mediated isothermal amplification method and MinION\u2122 nanopore sequencing","author":"Imai","year":"2017","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.exppara.2019.05.006_bib18","article-title":"Bench-scale experiments for the development of a unified loop-mediated isothermal amplification (LAMP) assay for the in vitro diagnosis of Leishmania species' promastigotes","author":"Karani","year":"2013","journal-title":"Epidemiol. Infect."},{"key":"10.1016\/j.exppara.2019.05.006_bib19","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1186\/1756-3305-5-280","article-title":"Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for detection of Leishmania DNA in buffy coat from visceral leishmaniasis patients","volume":"5","author":"Khan","year":"2012","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.exppara.2019.05.006_bib20","doi-asserted-by":"crossref","first-page":"68","DOI":"10.4038\/cmj.v61i2.8286","article-title":"Loop-mediated isothermal amplification assay as a sensitive diagnostic tool for Leishmania donovani infections in Sri Lanka","volume":"61","author":"Kothalawala","year":"2016","journal-title":"Ceylon Med. J."},{"key":"10.1016\/j.exppara.2019.05.006_bib21","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0006264","article-title":"Sensitive and less invasive confirmatory diagnosis of visceral leishmaniasis in Sudan using loop-mediated isothermal amplification (LAMP)","author":"Mukhtar","year":"2018","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.exppara.2019.05.006_bib22","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1006\/mcpr.2002.0415","article-title":"Accelerated reaction by loop-mediated isothermal amplification using loop primers","volume":"16","author":"Nagamine","year":"2002","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.exppara.2019.05.006_bib23","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"Notomi","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.exppara.2019.05.006_bib24","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.actatropica.2015.10.013","article-title":"A rapid molecular diagnosis of cutaneous leishmaniasis bycolorimetric malachite green-loop-mediated isothermal amplification (LAMP) combined with an FTA card as a direct sampling tool","volume":"153","author":"Nzelu","year":"2016","journal-title":"Acta Trop."},{"key":"10.1016\/j.exppara.2019.05.006_bib25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.actatropica.2013.12.016","article-title":"Development of a loop-mediated isothermal amplification method for rapid mass-screening of sand flies for Leishmania infection","volume":"132","author":"Nzelu","year":"2014","journal-title":"Acta Trop."},{"key":"10.1016\/j.exppara.2019.05.006_bib26","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1590\/s1984-29612016071","article-title":"Natural canine infection by Leishmania infantum and Leishmania amazonensis and their implications for disease control","volume":"25","author":"Sanches","year":"2016","journal-title":"Braz. J. Vet. Parasitol."},{"key":"10.1016\/j.exppara.2019.05.006_bib27","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/S0732-8893(03)00093-2","article-title":"PCR diagnosis and characterization of Leishmania in local and imported clinical samples","volume":"47","author":"Sch\u00f5nian","year":"2003","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.exppara.2019.05.006_bib28","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/S0001-706X(99)00036-4","article-title":"Characterization of kinetoplast DNA minicircles of an Indian isolate of Leishmania donovani","volume":"73","author":"Singh","year":"1999","journal-title":"Acta Trop."},{"key":"10.1016\/j.exppara.2019.05.006_bib29","doi-asserted-by":"crossref","DOI":"10.1186\/s13071-015-1202-x","article-title":"Development of loop-mediated isothermal amplification (LAMP) for simple detection of Leishmania infection","author":"Sriworarat","year":"2015","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.exppara.2019.05.006_bib30","doi-asserted-by":"crossref","first-page":"578","DOI":"10.4269\/ajtmh.2009.09-0145","article-title":"Sensitive, specific, and rapid detection of Leishmania donovani DNA by loop-mediated isothermal amplification","volume":"81","author":"Takagi","year":"2009","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.exppara.2019.05.006_bib31","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1590\/0074-02760160254","article-title":"Comparison of LAMP and PCR for molecular mass screening of sand flies for Leishmania martiniquensis infection","volume":"112","author":"Tiwananthagorn","year":"2017","journal-title":"Mem. Inst. Oswaldo Cruz"},{"issue":"3\u20134","key":"10.1016\/j.exppara.2019.05.006_bib32","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.vetpar.2007.07.008","article-title":"The first records of Leishmania (Leishmania) amazonensis in dogs (Canis familiaris) diagnosed clinically as having canine visceral leishmaniasis from Ara\u00e7atuba County, S\u00e3o Paulo State, Brazil","volume":"149","author":"Tolezano","year":"2007","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.exppara.2019.05.006_bib33","doi-asserted-by":"crossref","DOI":"10.1038\/srep40804","article-title":"Comparative genomics of canine isolated Leishmania (Leishmania) amazonensis from an endemic focus of visceral leishmaniasis in Governador Valadares, southeastern Brazil","author":"Valdivia","year":"2017","journal-title":"Sci. Rep."},{"issue":"2","key":"10.1016\/j.exppara.2019.05.006_bib34","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1128\/CMR.00104-14","article-title":"Species typing in dermal leishmaniasis","volume":"28","author":"Van der Auwera","year":"2015","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.exppara.2019.05.006_bib35","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/j.diagmicrobio.2013.01.011","article-title":"Application of loop-mediated isothermal amplification assay for the sensitive and rapid diagnosis of visceral leishmaniasis and post-kala-azar dermal leishmaniasis","volume":"75","author":"Verma","year":"2013","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.exppara.2019.05.006_bib36","doi-asserted-by":"crossref","DOI":"10.1186\/s12879-017-2318-8","article-title":"Development of a rapid loop-mediated isothermal amplification assay for diagnosis and assessment of cure of Leishmania infection","author":"Verma","year":"2017","journal-title":"BMC Infect. Dis."},{"issue":"11","key":"10.1016\/j.exppara.2019.05.006_bib37","doi-asserted-by":"crossref","first-page":"3892","DOI":"10.1128\/JCM.r00764-11","article-title":"Serial quantitative PCR assay for detection, species discrimination, and quantification of Leishmania spp. in human samples","volume":"49","author":"Weirather","year":"2011","journal-title":"J. Clin. Microbiol."}],"container-title":["Experimental Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489419300384?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489419300384?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,12,15]],"date-time":"2020-12-15T22:32:21Z","timestamp":1608071541000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014489419300384"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8]]},"references-count":37,"alternative-id":["S0014489419300384"],"URL":"https:\/\/doi.org\/10.1016\/j.exppara.2019.05.006","relation":{},"ISSN":["0014-4894"],"issn-type":[{"value":"0014-4894","type":"print"}],"subject":[],"published":{"date-parts":[[2019,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of Leishmania amazonensis in skin samples","name":"articletitle","label":"Article Title"},{"value":"Experimental Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exppara.2019.05.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}