{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:40:15Z","timestamp":1790509215734,"version":"4.1.0"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T00:00:00Z","timestamp":1661904000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Parasitology"],"published-print":{"date-parts":[[2022,10]]},"DOI":"10.1016\/j.exppara.2022.108353","type":"journal-article","created":{"date-parts":[[2022,8,19]],"date-time":"2022-08-19T11:06:05Z","timestamp":1660907165000},"page":"108353","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Effects of selected condensed tannins on Cryptosporidium parvum growth and proliferation in HCT-8\u00a0cell cultures"],"prefix":"10.1016","volume":"241","author":[{"given":"Ian David","family":"Woolsey","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wayne E.","family":"Zeller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1429-4305","authenticated-orcid":false,"given":"Berit Marie","family":"Blomstrand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8661-9765","authenticated-orcid":false,"given":"\u00d8ivind","family":"\u00d8ines","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7858-5745","authenticated-orcid":false,"given":"Heidi L.","family":"Enemark","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.exppara.2022.108353_bib1","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/j.exppara.2012.04.021","article-title":"Pomegranate (Punica granatum) peel is effective in a murine model of experimental Cryptosporidium parvum","volume":"131","author":"Al-Mathal","year":"2012","journal-title":"Exp. Parasitol."},{"issue":"6","key":"10.1016\/j.exppara.2022.108353_bib2","doi-asserted-by":"crossref","first-page":"1919","DOI":"10.1128\/aem.56.6.1919-1925.1990","article-title":"Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations","volume":"56","author":"Amann","year":"1990","journal-title":"Appl. Environ. Microbiol."},{"issue":"8","key":"10.1016\/j.exppara.2022.108353_bib3","doi-asserted-by":"crossref","first-page":"2919","DOI":"10.1007\/s00436-021-07220-w","article-title":"Extracts of pine bark (Pinus sylvestris) inhibit Cryptosporidium parvum growth in cell culture","volume":"120","author":"Blomstrand","year":"2021","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.exppara.2022.108353_bib4","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1017\/S0031182009990837","article-title":"Morphological characterization of Cryptosporidium parvum life-cycle stages in an in vitro model system","volume":"137","author":"Borowski","year":"2010","journal-title":"Parasitology"},{"issue":"36","key":"10.1016\/j.exppara.2022.108353_bib5","doi-asserted-by":"crossref","first-page":"8072","DOI":"10.1021\/acs.jafc.7b03489","article-title":"Facile purification of milligram to gram quantities of condensed tannins according to mean degree of polymerization and flavan-3-ol subunit composition","volume":"65","author":"Brown","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.exppara.2022.108353_bib6","series-title":"The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments","author":"Bustin","year":"2009"},{"issue":"11","key":"10.1016\/j.exppara.2022.108353_bib7","doi-asserted-by":"crossref","first-page":"3488","DOI":"10.1128\/aem.58.11.3488-3493.1992","article-title":"Viability of Cryptosporidium parvum oocysts: correlation of in vitro excystation with inclusion or exclusion of fluorogenic vital dyes","volume":"58","author":"Campbell","year":"1992","journal-title":"Appl. Environ. Microbiol."},{"issue":"5","key":"10.1016\/j.exppara.2022.108353_bib8","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.1111\/j.1365-2672.2010.04764.x","article-title":"Zoonotic cryptosporidiosis in the UK\u2013challenges for control","volume":"109","author":"Chalmers","year":"2010","journal-title":"J. Appl. Microbiol."},{"issue":"4","key":"10.1016\/j.exppara.2022.108353_bib9","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1017\/S0031182015001912","article-title":"Impact of chemical structure of flavanol monomers and condensed tannins on in vitro anthelmintic activity against bovine nematodes","volume":"143","author":"Desrues","year":"2016","journal-title":"Parasitology"},{"issue":"1","key":"10.1016\/j.exppara.2022.108353_bib10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13071-016-1617-z","article-title":"Anti-parasitic activity of pelleted sainfoin (Onobrychis viciifolia) against Ostertagia ostertagi and Cooperia oncophora in calves","volume":"9","author":"Desrues","year":"2016","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.exppara.2022.108353_bib11","volume":"vol. 1","author":"Derbakova","year":"2016"},{"issue":"1\u20132","key":"10.1016\/j.exppara.2022.108353_bib12","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.vetpar.2004.09.004","article-title":"Cryptosporidium: a water-borne zoonotic parasite","volume":"126","author":"Fayer","year":"2004","journal-title":"Vet. Parasitol."},{"issue":"2","key":"10.1016\/j.exppara.2022.108353_bib13","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.parint.2017.11.001","article-title":"Effect of oregano essential oil and carvacrol on Cryptosporidium parvum infectivity in HCT-8 cells","volume":"67","author":"Gaur","year":"2018","journal-title":"Parasitol. Int."},{"issue":"10","key":"10.1016\/j.exppara.2022.108353_bib14","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1016\/S0020-7519(01)00212-0","article-title":"Complete development and long-term maintenance of Cryptosporidium parvum human and cattle genotypes in cell culture","volume":"31","author":"Hijjawi","year":"2001","journal-title":"Int. J. Parasitol."},{"issue":"3","key":"10.1016\/j.exppara.2022.108353_bib15","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1093\/jac\/dkg357","article-title":"Effect of antiretroviral protease inhibitors alone, and in combination with paromomycin, on the excystation, invasion and in vitro development of Cryptosporidium parvum","volume":"52","author":"Hommer","year":"2003","journal-title":"J. Antimicrob. Chemother."},{"issue":"1\u20132","key":"10.1016\/j.exppara.2022.108353_bib16","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.vetpar.2011.11.042","article-title":"Direct and indirect effects of bioactive tannin-rich tropical and temperate legumes against nematode infections","volume":"186","author":"Hoste","year":"2012","journal-title":"Vet. Parasitol."},{"issue":"1\u20132","key":"10.1016\/j.exppara.2022.108353_bib17","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.vetpar.2015.06.026","article-title":"Tannin containing legumes as a model for nutraceuticals against digestive parasites in livestock","volume":"212","author":"Hoste","year":"2015","journal-title":"Vet. Parasitol."},{"issue":"1","key":"10.1016\/j.exppara.2022.108353_bib18","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1128\/CMR.15.1.145-154.2002","article-title":"Epidemiology and clinical features of Cryptosporidium infection in immunocompromised patients","volume":"15","author":"Hunter","year":"2002","journal-title":"Clin. Microbiol. Rev."},{"issue":"5","key":"10.1016\/j.exppara.2022.108353_bib19","doi-asserted-by":"crossref","first-page":"2505","DOI":"10.1128\/AEM.69.5.2505-2511.2003","article-title":"Cell culture-Taqman PCR assay for evaluation of Cryptosporidium parvum disinfection","volume":"69","author":"Keegan","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"issue":"1\u20133","key":"10.1016\/j.exppara.2022.108353_bib20","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0169-409X(96)00423-1","article-title":"Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings","volume":"23","author":"Lipinski","year":"1997","journal-title":"Adv. Drug Deliv. Rev."},{"issue":"4","key":"10.1016\/j.exppara.2022.108353_bib21","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.ddtec.2004.11.007","article-title":"Lead-and drug-like compounds: the rule-of-five revolution","volume":"1","author":"Lipinski","year":"2004","journal-title":"Drug Discov. Today Technol."},{"key":"10.1016\/j.exppara.2022.108353_bib22","series-title":"Annals of the New York","first-page":"102","article-title":"Cryptosporidiois: a global challenge","volume":"vol. 916","author":"Moisier","year":"2000"},{"issue":"5","key":"10.1016\/j.exppara.2022.108353_bib23","doi-asserted-by":"crossref","first-page":"825","DOI":"10.2307\/3284275","article-title":"Differentiation between human and animal isolates of Cryptosporidium parvum using rDNA sequencing and direct PCR analysis","volume":"83","author":"Morgan","year":"1997","journal-title":"J. Parasitol."},{"issue":"9","key":"10.1016\/j.exppara.2022.108353_bib24","doi-asserted-by":"crossref","first-page":"2123","DOI":"10.3390\/molecules23092123","article-title":"Relationships between structures of condensed tannins from Texas legumes and methane production during in vitro rumen digestion","volume":"23","author":"Naumann","year":"2018","journal-title":"Molecules"},{"issue":"2\u20134","key":"10.1016\/j.exppara.2022.108353_bib25","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.vetpar.2011.06.003","article-title":"Condensed tannins act against cattle nematodes","volume":"182","author":"Novobilsk\u00fd","year":"2011","journal-title":"Vet. Parasitol."},{"issue":"6","key":"10.1016\/j.exppara.2022.108353_bib27","doi-asserted-by":"crossref","first-page":"363","DOI":"10.17236\/sat00163","article-title":"The significance of cryptosporidiosis for the health of calves in Switzerland","volume":"160","author":"Olias","year":"2018","journal-title":"Schweizer Archiv fur Tierheilkunde"},{"issue":"1","key":"10.1016\/j.exppara.2022.108353_bib28","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1007\/s12639-014-0455-1","article-title":"Effect of condensed tannins supplementation through leaf meal mixture on voluntary feed intake, immune response and worm burden in Haemonchus contortus infected sheep","volume":"40","author":"Pathak","year":"2016","journal-title":"J. Parasit. Dis."},{"issue":"4","key":"10.1016\/j.exppara.2022.108353_bib29","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1128\/AAC.42.4.843","article-title":"Cyclosporin analogs inhibit in vitro growth of Cryptosporidium parvum","volume":"42","author":"Perkins","year":"1998","journal-title":"Antimicrob. Agents Chemother."},{"issue":"3","key":"10.1016\/j.exppara.2022.108353_bib30","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1089\/fpd.2017.2348","article-title":"Viability assessment of Cryptosporidium parvum oocysts by vital dyes: dry mounts overestimate the number of \u201cGhost\u201d oocysts","volume":"15","author":"Petersen","year":"2018","journal-title":"Foodborne Pathogens and Disease"},{"issue":"8","key":"10.1016\/j.exppara.2022.108353_bib31","doi-asserted-by":"crossref","first-page":"3809","DOI":"10.1128\/AEM.68.8.3809-3817.2002","article-title":"Comparison of in vitro cell culture and a mouse assay for measuring infectivity of Cryptosporidium parvum","volume":"68","author":"Rochelle","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"issue":"11","key":"10.1016\/j.exppara.2022.108353_bib32","doi-asserted-by":"crossref","first-page":"3494","DOI":"10.1128\/aem.58.11.3494-3500.1992","article-title":"Survival of Cryptosporidium parvum oocysts under various environmental pressures","volume":"58","author":"Robertson","year":"1992","journal-title":"Appl. Environ. Microbiol."},{"issue":"4","key":"10.1016\/j.exppara.2022.108353_bib33","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1007\/s00436-009-1535-5","article-title":"Effects of curcumin on Cryptosporidium parvum in vitro","volume":"105","author":"Shahiduzzaman","year":"2009","journal-title":"Parasitol. Res."},{"issue":"9","key":"10.1016\/j.exppara.2022.108353_bib34","doi-asserted-by":"crossref","first-page":"3669","DOI":"10.1128\/aem.63.9.3669-3675.1997","article-title":"An in vitro method for detecting infectious Cryptosporidium oocysts with cell culture","volume":"63","author":"Slifko","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"issue":"3\u20134","key":"10.1016\/j.exppara.2022.108353_bib35","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.anifeedsci.2011.07.004","article-title":"Effects of condensed tannins from Leucaena on methane production, rumen fermentation and populations of methanogens and protozoa in vitro","volume":"169","author":"Tan","year":"2011","journal-title":"Anim. Feed Sci. Technol."},{"issue":"1","key":"10.1016\/j.exppara.2022.108353_bib36","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1007\/s00436-012-2824-y","article-title":"In vitro determination of anticryptosporidial activity of phytogenic extracts and compounds","volume":"111","author":"Teichmann","year":"2012","journal-title":"Parasitol. Res."},{"issue":"1","key":"10.1016\/j.exppara.2022.108353_bib37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13567-017-0447-0","article-title":"Bovine cryptosporidiosis: impact, host-parasite interaction and control strategies","volume":"48","author":"Thomson","year":"2017","journal-title":"Vet. Res."},{"issue":"9","key":"10.1016\/j.exppara.2022.108353_bib38","doi-asserted-by":"crossref","first-page":"5800","DOI":"10.3168\/jds.2013-7136","article-title":"Effect of pomegranate-residue supplement on Cryptosporidium parvum oocyst shedding in neonatal calves","volume":"97","author":"Weyl-Feinstein","year":"2014","journal-title":"J. Dairy Sci."},{"issue":"5","key":"10.1016\/j.exppara.2022.108353_bib39","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0097053","article-title":"Direct anthelmintic effects of condensed tannins from diverse plant sources against Ascaris suum","volume":"9","author":"Williams","year":"2014","journal-title":"PLoS One"},{"issue":"1","key":"10.1016\/j.exppara.2022.108353_bib40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-019-56619-0","article-title":"Anti-protozoal activity of extracts from chicory (Cichorium intybus) against Cryptosporidium parvum in cell culture","volume":"9","author":"Woolsey","year":"2019","journal-title":"Sci. Rep."},{"issue":"1","key":"10.1016\/j.exppara.2022.108353_bib41","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13071-019-3851-7","article-title":"Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR","volume":"12","author":"Woolsey","year":"2019","journal-title":"Parasites Vectors"},{"issue":"4","key":"10.1016\/j.exppara.2022.108353_bib42","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1021\/jf504715p","article-title":"Protein precipitation behavior of condensed tannins from Lotus pedunculatus and Trifolium repens with different mean degrees of polymerization","volume":"63","author":"Zeller","year":"2015","journal-title":"J. Agric. Food Chem."},{"issue":"7","key":"10.1016\/j.exppara.2022.108353_bib43","doi-asserted-by":"crossref","first-page":"1967","DOI":"10.1021\/jf504743b","article-title":"1H\u201313C HSQC NMR spectroscopy for estimating procyanidin\/prodelphinidin and cis\/trans-flavan-3-ol ratios of condensed tannin samples: correlation with thiolysis","volume":"63","author":"Zeller","year":"2015","journal-title":"J. Agric. Food Chem."},{"issue":"7","key":"10.1016\/j.exppara.2022.108353_bib44","doi-asserted-by":"crossref","first-page":"2016","DOI":"10.1021\/acs.jafc.9b06173","article-title":"Comparison of protein precipitation ability of structurally diverse Procyanidin-rich condensed tannins in two buffer systems","volume":"68","author":"Zeller","year":"2020","journal-title":"J. Agric. Food Chem."}],"container-title":["Experimental Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489422001473?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489422001473?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T06:03:33Z","timestamp":1762149813000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014489422001473"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10]]},"references-count":43,"alternative-id":["S0014489422001473"],"URL":"https:\/\/doi.org\/10.1016\/j.exppara.2022.108353","relation":{},"ISSN":["0014-4894"],"issn-type":[{"value":"0014-4894","type":"print"}],"subject":[],"published":{"date-parts":[[2022,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Effects of selected condensed tannins on Cryptosporidium parvum growth and proliferation in HCT-8\u00a0cell cultures","name":"articletitle","label":"Article Title"},{"value":"Experimental Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exppara.2022.108353","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"108353"}}