{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T08:34:42Z","timestamp":1768466082327,"version":"3.49.0"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T00:00:00Z","timestamp":1721088000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003762","name":"Institut Pasteur","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003762","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Parasitology"],"published-print":{"date-parts":[[2024,8]]},"DOI":"10.1016\/j.exppara.2024.108803","type":"journal-article","created":{"date-parts":[[2024,7,14]],"date-time":"2024-07-14T10:13:44Z","timestamp":1720952024000},"page":"108803","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Production and evaluation of a new set of recombinant antigens for the serological diagnosis of human cysticercosis"],"prefix":"10.1016","volume":"263-264","author":[{"given":"Jihen","family":"Melki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thierry-Borel N'dri","family":"Kouadio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mireille","family":"Nowakowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zara","family":"Razafiarimanga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Man-Koumba","family":"Soumahoro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephane","family":"Peltres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9908-1378","authenticated-orcid":false,"given":"Ronan","family":"Jambou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"6943","key":"10.1016\/j.exppara.2024.108803_bib1","doi-asserted-by":"crossref","first-page":"1552","DOI":"10.1136\/bmj.308.6943.1552","article-title":"Diagnostic tests. 1: sensitivity and specificity","volume":"308","author":"Altman","year":"1994","journal-title":"BMJ (Clinical research ed.)"},{"issue":"1","key":"10.1016\/j.exppara.2024.108803_bib2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.pep.2005.12.002","article-title":"Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins","volume":"48","author":"Arnau","year":"2006","journal-title":"Protein Expr. Purif."},{"issue":"7","key":"10.1016\/j.exppara.2024.108803_bib3","doi-asserted-by":"crossref","first-page":"953","DOI":"10.4014\/jmb.1412.12079","article-title":"Production of biopharmaceuticals in E. coli: current scenario and future Perspectives","volume":"25","author":"Baeshen","year":"2015","journal-title":"J. Microbiol. Biotechnol."},{"issue":"3","key":"10.1016\/j.exppara.2024.108803_bib4","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1021\/bp070011x","article-title":"Enhanced bacterial protein expression during auto-induction obtained by alteration of lac repressor dosage and medium composition","volume":"23","author":"Blommel","year":"2007","journal-title":"Biotechnol. Prog."},{"issue":"5","key":"10.1016\/j.exppara.2024.108803_bib5","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1371\/journal.pntd.0001152","article-title":"Clinical manifestations associated with neurocysticercosis: a Systematic review","volume":"5","author":"Carabin","year":"2011","journal-title":"PLoS Neglected Trop. Dis."},{"issue":"1","key":"10.1016\/j.exppara.2024.108803_bib6","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0378-1097(98)00444-3","article-title":"Dynamics of in vivo protein aggregation: building inclusion bodies in recombinant bacteria","volume":"169","author":"Carri\u00f3","year":"1998","journal-title":"FEMS Microbiol. Lett."},{"issue":"2","key":"10.1016\/j.exppara.2024.108803_bib7","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/S0732-8893(00)00137-1","article-title":"A quantitative enzyme-linked immunosorbent assay (ELISA) for the immunodiagnosis of neurocysticercosis using a purified fraction from Taenia solium cysticerci","volume":"37","author":"da Silva","year":"2000","journal-title":"Diagn. Microbiol. Infect. Dis."},{"issue":"3","key":"10.1016\/j.exppara.2024.108803_bib8","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.actatropica.2006.10.009","article-title":"Recombinant expression of Taenia solium TS14 antigen and its utilization for immunodiagnosis of neurocysticercosis","volume":"100","author":"da Silva","year":"2006","journal-title":"Acta Trop."},{"issue":"1\u20132","key":"10.1016\/j.exppara.2024.108803_bib9","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/0304-4017(88)90019-2","article-title":"The histopathology of porcine cysticercosis","volume":"28","author":"de Aluja","year":"1988","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.exppara.2024.108803_bib10","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1186\/1472-6750-7-32","article-title":"Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli","volume":"7","author":"De Marco","year":"2007","journal-title":"BMC Biotechnol."},{"issue":"2","key":"10.1016\/j.exppara.2024.108803_bib11","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1212\/WNL.57.2.177","article-title":"Proposed diagnostic criteria for neurocysticercosis","volume":"57","author":"Del Brutto","year":"2001","journal-title":"Neurology"},{"issue":"3","key":"10.1016\/j.exppara.2024.108803_bib12","first-page":"151","article-title":"Hallazgos neuropatol\u00f3gicos en la neurocisticercosis [Neuropathologic findings in neurocysticercosis]","volume":"48","author":"Escobar","year":"1985","journal-title":"Arch. Neurobiol."},{"key":"10.1016\/j.exppara.2024.108803_bib13","first-page":"77","article-title":"Taeniasis and cysticercosis due to Taenia solium","volume":"4","author":"Flisser","year":"1994","journal-title":"Prog. Clin. Parasitol."},{"issue":"9383","key":"10.1016\/j.exppara.2024.108803_bib14","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/S0140-6736(03)14117-7","article-title":"Taenia solium cysticercosis","volume":"362","author":"Garc\u00eda","year":"2003","journal-title":"Lancet"},{"issue":"12","key":"10.1016\/j.exppara.2024.108803_bib15","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.1016\/S1474-4422(14)70094-8","article-title":"Clinical symptoms, diagnosis, and treatment of neurocysticercosis","volume":"13","author":"Garcia","year":"2014","journal-title":"Lancet Neurol."},{"issue":"2","key":"10.1016\/j.exppara.2024.108803_bib16","doi-asserted-by":"crossref","first-page":"152","DOI":"10.4269\/ajtmh.1994.50.152","article-title":"Discrepancies between cerebral Computed tomography and western blot in the diagnosis of neurocysticercosis","volume":"50","author":"Garc\u013aa","year":"1994","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"5","key":"10.1016\/j.exppara.2024.108803_bib17","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1645\/0022-3395(2000)086[1001:TSMCAS]2.0.CO;2","article-title":"Taenia solium: molecular cloning and serologic evaluation of 14- and 18-kda related, diagnostic antigens","volume":"86","author":"Greene","year":"2000","journal-title":"J. Parasitol."},{"issue":"2","key":"10.1016\/j.exppara.2024.108803_bib18","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/S0166-6851(99)00004-3","article-title":"Diagnostic glycoproteins of Taenia solium cysts share homologous 14- and 18-kDa subunits","volume":"99","author":"Greene","year":"1999","journal-title":"Mol. Biochem. Parasitol."},{"issue":"1","key":"10.1016\/j.exppara.2024.108803_bib19","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1080\/15321810903405068","article-title":"Development and evaluation of porcine cysticercosis QuickELISA in Triturus EIA analyzer","volume":"31","author":"Handali","year":"2010","journal-title":"J. Immunoassay Immunochem."},{"key":"10.1016\/j.exppara.2024.108803_bib20","doi-asserted-by":"crossref","DOI":"10.1016\/j.actatropica.2021.106294","article-title":"Evaluation of recombinant glutathione transferase 26 kDa, thioredoxin-1, and endophilin B1 of Taenia solium in the diagnosis of human neurocysticercosis","volume":"227","author":"Jim\u00e9nez","year":"2022","journal-title":"Acta Trop."},{"issue":"6","key":"10.1016\/j.exppara.2024.108803_bib21","doi-asserted-by":"crossref","first-page":"2411","DOI":"10.1007\/s00253-020-10402-8","article-title":"Fusion tags to enhance heterologous protein expression","volume":"104","author":"Ki","year":"2020","journal-title":"Appl. Microbiol. Biotechnol."},{"issue":"2","key":"10.1016\/j.exppara.2024.108803_bib22","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1148\/radiology.171.2.2704811","article-title":"Cerebral cysticercosis: documentation of natural history with CT","volume":"171","author":"Kramer","year":"1989","journal-title":"Radiology"},{"issue":"4","key":"10.1016\/j.exppara.2024.108803_bib23","doi-asserted-by":"crossref","first-page":"587","DOI":"10.4269\/ajtmh.2011.10-0079","article-title":"Serologic diagnosis of human Taenia solium cysticercosis by using recombinant and synthetic antigens in QuickELISATM","volume":"84","author":"Lee","year":"2011","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"1","key":"10.1016\/j.exppara.2024.108803_bib24","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1110\/ps.051812706","article-title":"Comparison of SUMO fusion technology with traditional gene fusion systems: enhanced expression and solubility with SUMO","volume":"15","author":"Marblestone","year":"2006","journal-title":"Protein Sci. : a publication of the Protein Society"},{"key":"10.1016\/j.exppara.2024.108803_bib25","doi-asserted-by":"crossref","first-page":"111321","DOI":"10.1016\/j.molbiopara.2020.111321","article-title":"Characterization and evaluation of three new recombinant antigens of Taenia solium for the immunodiagnosis of cysticercosis","volume":"240","author":"Morillo","year":"2020","journal-title":"Mol. Biochem. Parasitol."},{"issue":"11","key":"10.1016\/j.exppara.2024.108803_bib26","doi-asserted-by":"crossref","first-page":"2121","DOI":"10.3390\/diagnostics11112121","article-title":"Evaluation of an antibody detecting point of care test for diagnosis of Taenia solium cysticercosis in a Zambian rural community: a prospective diagnostic accuracy study","volume":"11","author":"Mubanga","year":"2021","journal-title":"Diagnostics"},{"key":"10.1016\/j.exppara.2024.108803_bib27","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.vetpar.2016.02.021","article-title":"Bio-guided identification of proteins for the diagnosis of cysticercosis in swine","volume":"220","author":"Nativel","year":"2016","journal-title":"Vet. Parasitol."},{"issue":"5","key":"10.1016\/j.exppara.2024.108803_bib28","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1017\/S0022149X15000747","article-title":"Performance of Tsol-p27 antigen for the serological diagnosis of cysticercosis in Mozambique","volume":"90","author":"Nhancupe","year":"2016","journal-title":"J. Helminthol."},{"issue":"4","key":"10.1016\/j.exppara.2024.108803_bib29","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1645\/GE-3046","article-title":"In situ detection of antigenic glycoproteins in Taenia solium metacestodes","volume":"89","author":"Obreg\u00f3n-Henao","year":"2003","journal-title":"J. Parasitol."},{"key":"10.1016\/j.exppara.2024.108803_bib30","doi-asserted-by":"crossref","first-page":"837","DOI":"10.3390\/bios13090837","article-title":"Lateral flow assay: a summary of recent progress for improving assay performance","volume":"13","author":"Omidfar","year":"2023","journal-title":"Biosensors"},{"issue":"4","key":"10.1016\/j.exppara.2024.108803_bib31","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1016\/S0020-7519(99)00021-1","article-title":"Vaccination against Taenia solium cysticercosis in pigs using native and recombinant oncosphere antigens","volume":"29","author":"Plancarte","year":"1999","journal-title":"Int. J. Parasitol."},{"issue":"1","key":"10.1016\/j.exppara.2024.108803_bib32","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1002\/biot.201100459","article-title":"The two faces of His-tag: immune response versus ease of protein purification","volume":"7","author":"Randolph","year":"2012","journal-title":"Biotechnol. J."},{"issue":"5","key":"10.1016\/j.exppara.2024.108803_bib33","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1179\/2047773212Y.0000000048","article-title":"Immunological and molecular diagnosis of cysticercosis","volume":"106","author":"Rodriguez","year":"2012","journal-title":"Pathog. Glob. Health"},{"key":"10.1016\/j.exppara.2024.108803_bib34","doi-asserted-by":"crossref","DOI":"10.1016\/j.fawpar.2023.e00211","article-title":"Development and evaluation of an immunochromatography-based point-of-care test kit for a rapid diagnosis of human cysticercosis","volume":"33","author":"Sadaow","year":"2023","journal-title":"Food and waterborne parasitology"},{"issue":"4","key":"10.1016\/j.exppara.2024.108803_bib35","doi-asserted-by":"crossref","first-page":"771","DOI":"10.4269\/ajtmh.2005.73.771","article-title":"Serodiagnosis of neurocysticercosis using synthetic 8-kD proteins: comparison of assay formats","volume":"73","author":"Scheel","year":"2005","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"14","key":"10.1016\/j.exppara.2024.108803_bib36","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1016\/j.ijpara.2013.07.009","article-title":"Immune responses to viable and degenerative metacestodes of Taenia solium in naturally infected swine","volume":"43","author":"Singh","year":"2013","journal-title":"Int. J. Parasitol."},{"issue":"2","key":"10.1016\/j.exppara.2024.108803_bib37","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1177\/1756285610395654","article-title":"Neurocysticercosis","volume":"4","author":"Singhi","year":"2011","journal-title":"Therapeutic Advances in Neurological Disorders"},{"issue":"8","key":"10.1016\/j.exppara.2024.108803_bib38","doi-asserted-by":"crossref","first-page":"1712","DOI":"10.3390\/microorganisms9081712","article-title":"Seroprevalence of cysticercosis among epileptic patients attending neurological units in the urban area of abidjan","volume":"9","author":"Soumahoro","year":"2021","journal-title":"Microorganisms"},{"issue":"1","key":"10.1016\/j.exppara.2024.108803_bib39","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.pep.2005.01.016","article-title":"Protein production by auto-induction in high density shaking cultures","volume":"41","author":"Studier","year":"2005","journal-title":"Protein Expr. Purif."},{"issue":"1","key":"10.1016\/j.exppara.2024.108803_bib40","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1093\/infdis\/159.1.50","article-title":"An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium)","volume":"159","author":"Tsang","year":"1989","journal-title":"JID (J. Infect. Dis.)"},{"issue":"4","key":"10.1016\/j.exppara.2024.108803_bib41","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/s11604-016-0528-4","article-title":"A comprehensive review of imaging findings in human cysticercosis","volume":"34","author":"Venkat","year":"2016","journal-title":"Jpn. J. Radiol."},{"issue":"6","key":"10.1016\/j.exppara.2024.108803_bib42","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1002\/bit.21218","article-title":"The conformational quality of insoluble recombinant proteins is enhanced at low growth temperatures","volume":"96","author":"Vera","year":"2007","journal-title":"Biotechnol. Bioeng."},{"issue":"5","key":"10.1016\/j.exppara.2024.108803_bib43","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1093\/infdis\/164.5.1007","article-title":"Clinical evaluation of the cysticercosis enzyme-linked immunoelectrotransfer blot in patients with neurocysticercosis","volume":"164","author":"Wilson","year":"1991","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.exppara.2024.108803_bib44","series-title":"Taenia Solium Taeniasis\/cysticercosis Fact Sheet","year":"2022"},{"key":"10.1016\/j.exppara.2024.108803_bib45","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2021.116452","article-title":"Point-of-care COVID-19 diagnostics powered by lateral flow assay","volume":"145","author":"Zhou","year":"2021","journal-title":"Trends in analytical chemistry : TRAC"}],"container-title":["Experimental Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489424001061?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489424001061?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T07:42:37Z","timestamp":1723534957000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014489424001061"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8]]},"references-count":45,"alternative-id":["S0014489424001061"],"URL":"https:\/\/doi.org\/10.1016\/j.exppara.2024.108803","relation":{},"ISSN":["0014-4894"],"issn-type":[{"value":"0014-4894","type":"print"}],"subject":[],"published":{"date-parts":[[2024,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Production and evaluation of a new set of recombinant antigens for the serological diagnosis of human cysticercosis","name":"articletitle","label":"Article Title"},{"value":"Experimental Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exppara.2024.108803","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"108803"}}