{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T17:37:55Z","timestamp":1792431475969,"version":"4.5.0"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,7,13]],"date-time":"2024-07-13T00:00:00Z","timestamp":1720828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100019174","name":"Australian Companion Animal Health Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100019174","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014013","name":"UKRI","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014013","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007456","name":"Canine Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007456","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Parasitology"],"published-print":{"date-parts":[[2024,8]]},"DOI":"10.1016\/j.exppara.2024.108806","type":"journal-article","created":{"date-parts":[[2024,7,14]],"date-time":"2024-07-14T08:59:10Z","timestamp":1720947550000},"page":"108806","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Whole genome amplification and sequencing of individual Dirofilaria immitis microfilariae"],"prefix":"10.1016","volume":"263-264","author":[{"given":"Rosemonde I.","family":"Power","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen R.","family":"Doyle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1459-9117","authenticated-orcid":false,"given":"Jan","family":"\u0160lapeta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.exppara.2024.108806_bib1","author":"Andrews"},{"key":"10.1016\/j.exppara.2024.108806_bib2","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1016\/j.ijpddr.2018.06.006","article-title":"Clinical validation of molecular markers of macrocyclic lactone resistance in Dirofilaria immitis","volume":"8","author":"Ballesteros","year":"2018","journal-title":"Int. J. Parasitol. Drugs Drug Resist."},{"key":"10.1016\/j.exppara.2024.108806_bib3","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.1093\/bioinformatics\/btr174","article-title":"BamTools: a C++ API and toolkit for analyzing and managing BAM files","volume":"27","author":"Barnett","year":"2011","journal-title":"Bioinformatics"},{"key":"10.1016\/j.exppara.2024.108806_bib4","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1186\/s13071-016-1476-7","article-title":"Efficacy of four commercially available heartworm preventive products against the JYD-34 laboratory strain of Dirofilaria immitis","volume":"9","author":"Blagburn","year":"2016","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.exppara.2024.108806_bib5","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.vetpar.2010.12.049","article-title":"Comparative efficacy of four commercially available heartworm preventive products against the MP3 laboratory strain of Dirofilaria immitis","volume":"176","author":"Blagburn","year":"2011","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.exppara.2024.108806_bib6","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.exppara.2024.108806_bib7","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1186\/s13071-017-2428-6","article-title":"Genetic profiles of ten Dirofilaria immitis isolates susceptible or resistant to macrocyclic lactone heartworm preventives","volume":"10","author":"Bourguinat","year":"2017","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.exppara.2024.108806_bib8","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.vetpar.2015.04.002","article-title":"Macrocyclic lactone resistance in Dirofilaria immitis: failure of heartworm preventives and investigation of genetic markers for resistance","volume":"210","author":"Bourguinat","year":"2015","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.exppara.2024.108806_bib9","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1186\/s13071-017-2437-5","article-title":"Dirofilaria immitis JYD-34 isolate: whole genome analysis","volume":"10","author":"Bourguinat","year":"2017","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.exppara.2024.108806_bib10","doi-asserted-by":"crossref","DOI":"10.1038\/nmicrobiol.2016.207","article-title":"Genomic diversity in Onchocerca volvulus and its Wolbachia endosymbiont","volume":"2","author":"Choi","year":"2016","journal-title":"Nature Microbiology"},{"key":"10.1016\/j.exppara.2024.108806_bib11","doi-asserted-by":"crossref","DOI":"10.1093\/gigascience\/giab008","article-title":"Twelve years of SAMtools and BCFtools","volume":"10","author":"Danecek","year":"2021","journal-title":"GigaScience"},{"key":"10.1016\/j.exppara.2024.108806_bib12","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1186\/s13071-023-05690-8","article-title":"Heartworm adulticide treatment: a tropical perspective","volume":"16","author":"Dantas-Torres","year":"2023","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.exppara.2024.108806_bib13","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.pt.2019.01.004","article-title":"Genome-wide approaches to investigate anthelmintic resistance","volume":"35","author":"Doyle","year":"2019","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.exppara.2024.108806_bib14","doi-asserted-by":"crossref","DOI":"10.3389\/fgene.2019.00826","article-title":"Evaluation of DNA extraction methods on individual helminth egg and larval stages for whole-genome sequencing","volume":"10","author":"Doyle","year":"2019","journal-title":"Front. Genet."},{"key":"10.1016\/j.exppara.2024.108806_bib15","doi-asserted-by":"crossref","first-page":"158","DOI":"10.2307\/3272537","article-title":"The isolation of microfilariae from blood for use as antigen","volume":"31","author":"Franks","year":"1945","journal-title":"J. Parasitol."},{"key":"10.1016\/j.exppara.2024.108806_bib16","series-title":"GNU Datamash","year":"2014"},{"key":"10.1016\/j.exppara.2024.108806_bib17","article-title":"Genome structure and population genomics of the canine heartworm Dirofilaria immitis","author":"Gandasegui","year":"2023","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.exppara.2024.108806_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetpar.2019.108995","article-title":"The prevalence of Dirofilaria immitis and D. repens in the old world","volume":"280","author":"Genchi","year":"2020","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.exppara.2024.108806_bib19","doi-asserted-by":"crossref","first-page":"4650","DOI":"10.1096\/fj.12-205096","article-title":"The genome of the heartworm, Dirofilaria immitis, reveals drug and vaccine targets","volume":"26","author":"Godel","year":"2012","journal-title":"Faseb. J."},{"key":"10.1016\/j.exppara.2024.108806_bib20","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.3390\/ani12111342","article-title":"De novo assembly of the Dirofilaria immitis genome by long-read nanopore-based sequencing technology on an adult worm from a canine cardiopulmonary dirofilariosis case","volume":"12","author":"Gomes-de-S\u00e1","year":"2022","journal-title":"Animals"},{"key":"10.1016\/j.exppara.2024.108806_bib21","author":"Kassambara"},{"key":"10.1016\/j.exppara.2024.108806_bib22","article-title":"Whole-genome reference of Dirofilaria immitis from Australia to determine single nucleotide polymorphisms associated with macrocyclic lactone resistance in the USA","volume":"1","author":"Lau","year":"2021","journal-title":"Curr. Res. Parasitol. Vector. Borne Dis."},{"key":"10.1016\/j.exppara.2024.108806_bib23","article-title":"Aligning sequence reads, clone sequences and assembly contigs with BWA-MEM","author":"Li","year":"2013","journal-title":"arXiv:1303.3997 [q-bio.GN]"},{"key":"10.1016\/j.exppara.2024.108806_bib24","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1186\/s13071-017-2429-5","article-title":"Efficacy of oral moxidectin against susceptible and resistant isolates of Dirofilaria immitis in dogs","volume":"10","author":"McTier","year":"2017","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.exppara.2024.108806_bib25","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1186\/s13071-016-1821-x","article-title":"Mosquito-borne heartworm Dirofilaria immitis in dogs from Australia","volume":"9","author":"Nguyen","year":"2016","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.exppara.2024.108806_bib26","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.ijpddr.2021.03.004","article-title":"Heartworm disease \u2013 overview, intervention, and industry perspective","volume":"16","author":"Noack","year":"2021","journal-title":"Int. J. Parasitol. Drugs Drug Resist."},{"key":"10.1016\/j.exppara.2024.108806_bib27","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1186\/s13071-020-3943-4","article-title":"Pig-hunting dogs are an at-risk population for canine heartworm (Dirofilaria immitis) infection in eastern Australia","volume":"13","author":"Orr","year":"2020","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.exppara.2024.108806_bib28","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1186\/s13071-021-04896-y","article-title":"Multiple diagnostic tests demonstrate an increased risk of canine heartworm disease in northern Queensland, Australia","volume":"14","author":"Panetta","year":"2021","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.exppara.2024.108806_bib29","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.ijpddr.2022.11.003","article-title":"Exploration of the sensitivity to macrocyclic lactones in the canine heartworm (Dirofilaria immitis) in Australia using phenotypic and genotypic approaches","volume":"20","author":"Power","year":"2022","journal-title":"Int. J. Parasitol. Drugs Drug Resist."},{"key":"10.1016\/j.exppara.2024.108806_bib30","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1186\/s13071-014-0494-6","article-title":"Establishment of macrocyclic lactone resistant Dirofilaria immitis isolates in experimentally infected laboratory dogs","volume":"7","author":"Pulaski","year":"2014","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.exppara.2024.108806_bib31","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1093\/bioinformatics\/btq033","article-title":"BEDTools: a flexible suite of utilities for comparing genomic features","volume":"26","author":"Quinlan","year":"2010","journal-title":"Bioinformatics"},{"key":"10.1016\/j.exppara.2024.108806_bib32","series-title":"R: A Language and Environment for Statistical Computing","year":"2023"},{"key":"10.1016\/j.exppara.2024.108806_bib33","doi-asserted-by":"crossref","first-page":"4811","DOI":"10.1038\/s41467-019-12695-4","article-title":"The global diversity of Haemonchus contortus is shaped by human intervention and climate","volume":"10","author":"Sall\u00e9","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.exppara.2024.108806_bib34","doi-asserted-by":"crossref","first-page":"39","DOI":"10.2307\/3275668","article-title":"Studies on the microfilariae of the dog heartworm Dirofilaria immitis: separation of parasites from whole blood","volume":"49","author":"Sawyer","year":"1963","journal-title":"J. Parasitol."},{"key":"10.1016\/j.exppara.2024.108806_bib35","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetpar.2020.109225","article-title":"Comparative sequences of the Wolbachia genomes of drug-sensitive and resistant isolates of Dirofilaria immitis","volume":"286","author":"Shin","year":"2020","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.exppara.2024.108806_bib36","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1128\/CMR.00012-12","article-title":"Human and animal dirofilariasis: the emergence of a zoonotic mosaic","volume":"25","author":"Sim\u00f3n","year":"2012","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.exppara.2024.108806_bib37","doi-asserted-by":"crossref","first-page":"1931","DOI":"10.1093\/molbev\/msz116","article-title":"Human migration and the spread of the nematode parasite Wuchereria bancrofti","volume":"36","author":"Small","year":"2019","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.exppara.2024.108806_bib38","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1017\/S0022149X00020290","article-title":"Studies on the microfilariae of Loa loa, Wuchereria bancrofti, Brugia malayi, Dirofilaria immitis, D. repens and D. aethiops","volume":"34","author":"Taylor","year":"1960","journal-title":"J. Helminthol."},{"key":"10.1016\/j.exppara.2024.108806_bib39","series-title":"Stringr: Simple, Consistent Wrappers for Common String Operations","author":"Wickham","year":"2023"},{"key":"10.1016\/j.exppara.2024.108806_bib40","doi-asserted-by":"crossref","first-page":"1686","DOI":"10.21105\/joss.01686","article-title":"Welcome to the tidyverse","volume":"4","author":"Wickham","year":"2019","journal-title":"J. Open Source Softw."},{"key":"10.1016\/j.exppara.2024.108806_bib41","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1017\/S003118201500061X","article-title":"The emergence of macrocyclic lactone resistance in the canine heartworm, Dirofilaria immitis","volume":"142","author":"Wolstenholme","year":"2015","journal-title":"Parasitology"},{"key":"10.1016\/j.exppara.2024.108806_bib42","series-title":"Ggsci: Scientific Journal and Sci-Fi Themed Color Palettes for 'ggplot2","author":"Xiao","year":"2024"}],"container-title":["Experimental Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489424001097?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489424001097?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T07:42:39Z","timestamp":1723534959000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014489424001097"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8]]},"references-count":42,"alternative-id":["S0014489424001097"],"URL":"https:\/\/doi.org\/10.1016\/j.exppara.2024.108806","relation":{},"ISSN":["0014-4894"],"issn-type":[{"value":"0014-4894","type":"print"}],"subject":[],"published":{"date-parts":[[2024,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Whole genome amplification and sequencing of individual Dirofilaria immitis microfilariae","name":"articletitle","label":"Article Title"},{"value":"Experimental Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exppara.2024.108806","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"108806"}}