{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T22:51:56Z","timestamp":1791327116230,"version":"4.3.1"},"reference-count":32,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004612","name":"Araucaria Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004612","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["CNPq-406572\/2022-4"],"award-info":[{"award-number":["CNPq-406572\/2022-4"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["404898\/2024-6"],"award-info":[{"award-number":["404898\/2024-6"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["408391\/2022-7"],"award-info":[{"award-number":["408391\/2022-7"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Experimental Parasitology"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.exppara.2026.109209","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T15:36:55Z","timestamp":1789745815000},"page":"109209","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Evaluation of tissue cyst protection in pigs immunized by intradermal and intramuscular routes with a DNA vaccine (rop18 + sag1) against Toxoplasma gondii"],"prefix":"10.1016","volume":"290","author":[{"given":"Ana Paula Molinari","family":"Candeias","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Cl\u00e9cia","family":"dos Santos Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafaela Maria Bolson","family":"Jurkevicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Carolina","family":"Cavallieri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Pedro","family":"Sasse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"de Souza Lima Nino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando Pietrini","family":"Soufen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"de Souza Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Daniel","family":"de Barros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1826-7582","authenticated-orcid":false,"given":"Jo\u00e3o Lu\u00eds","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"3-4","key":"10.1016\/j.exppara.2026.109209_bib1","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/S0264-410X(99)00329-1","article-title":"Predictive estimation of protein linear epitopes by using the program PEOPLE","author":"Alix","year":"1999","journal-title":"Vaccine"},{"key":"10.1016\/j.exppara.2026.109209_bib2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetpar.2019.108963","article-title":"Cell mediated and innate immune responses in pigs following vaccination and challenge with Toxoplasma parasites","volume":"275","author":"Bartley","year":"2019","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.exppara.2026.109209_bib3","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1186\/s13567-015-0177-0","article-title":"Vaccination of pigs with the S48 strain of Toxoplasma gondii\u2014safer meat for human consumption","volume":"46","author":"Burrells","year":"2015","journal-title":"Vet. Res."},{"key":"10.1016\/j.exppara.2026.109209_bib4","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1136\/vr.129.5.89","article-title":"Vaccination of sheep with a live incomplete strain (S48) of Toxoplasma gondii and their immunity to challenge when pregnant","volume":"129","author":"Buxton","year":"1991","journal-title":"Veterinary Record"},{"key":"10.1016\/j.exppara.2026.109209_bib5","first-page":"143","article-title":"Introdu\u00e7\u00e3o \u00e0s t\u00e9cnicas de imunofluoresc\u00eancia","volume":"10","author":"Camargo","year":"1973","journal-title":"Revista Brasileira de Patologia Cl\u00ednica"},{"key":"10.1016\/j.exppara.2026.109209_bib6","doi-asserted-by":"crossref","first-page":"6659","DOI":"10.1016\/j.vaccine.2020.08.054","article-title":"Adjuvants for swine vaccines: mechanisms of action and adjuvant effects","volume":"38","author":"Charerntantanakul","year":"2020","journal-title":"Vaccine"},{"key":"10.1016\/j.exppara.2026.109209_bib7","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.vetpar.2011.11.034","article-title":"Humoral and cellular immune responses in pigs immunized intranasally with crude rhoptry proteins of Toxoplasma gondii plus Quil-A","volume":"186","author":"Cunha","year":"2012","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.exppara.2026.109209_bib8","doi-asserted-by":"crossref","DOI":"10.1590\/s1984-29612020077","article-title":"Protection against Toxoplasma gondii cysts in pigs immunized with rROP2 plus Iscomatrix","volume":"29","author":"Cunha","year":"2020","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"10.1016\/j.exppara.2026.109209_bib9","doi-asserted-by":"crossref","first-page":"749","DOI":"10.2307\/3284582","article-title":"Immunity to toxoplasmosis in pigs fed irradiated Toxoplasma gondii oocysts","volume":"84","author":"Dubey","year":"1998","journal-title":"J. Parasitol."},{"key":"10.1016\/j.exppara.2026.109209_bib10","doi-asserted-by":"crossref","DOI":"10.1128\/CMR.00057-17","article-title":"Treatment of toxoplasmosis: historical perspective, animal models, and current clinical practice","volume":"31","author":"Dunay","year":"2018","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.exppara.2026.109209_bib11","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1590\/S0102-09352003000400002","article-title":"Vaccination of pigs with Toxoplasma gondii antigens incorporated in immunostimulating complexes (ISCOMs)","volume":"55","author":"Freire","year":"2003","journal-title":"Arq. Bras. Med. Vet. Zootec."},{"key":"10.1016\/j.exppara.2026.109209_bib12","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.rvsc.2007.04.014","article-title":"Evaluation of serological tests for Toxoplasma gondii detection","volume":"84","author":"Garcia","year":"2008","journal-title":"Res. Vet. Sci."},{"key":"10.1016\/j.exppara.2026.109209_bib13","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1016\/j.rvsc.2017.07.022","article-title":"Diagnosis of ocular toxoplasmosis in pigs","volume":"115","author":"Garcia","year":"2017","journal-title":"Res. Vet. Sci."},{"key":"10.1016\/j.exppara.2026.109209_bib14","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.vetpar.2005.01.006","article-title":"Partial protection against tissue cyst formation in pigs vaccinated with crude rhoptry proteins","volume":"129","author":"Garcia","year":"2005","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.exppara.2026.109209_bib15","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1590\/S0103-84781999000100017","article-title":"Seroprevalence of Toxoplasma gondii in farm animals and humans","volume":"29","author":"Garcia","year":"1999","journal-title":"Ci\u00eancia Rural."},{"key":"10.1016\/j.exppara.2026.109209_bib16","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.exppara.2005.12.011","article-title":"Comparison of ELISA with IFAT and MAT in pigs","volume":"113","author":"Garcia","year":"2006","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.exppara.2026.109209_bib17","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/j.exppara.2007.10.002","article-title":"Toxoplasma gondii: evaluation of an intranasal vaccine using recombinant proteins against brain cyst formation in BALB\/c mice","volume":"118","author":"Igarashi","year":"2008","journal-title":"Exp. Parasitol."},{"issue":"1","key":"10.1016\/j.exppara.2026.109209_bib18","doi-asserted-by":"crossref","first-page":"37","DOI":"10.3347\/PHD.24082","article-title":"Anti-tumor effects of Toxoplasma gondii and antigen-pulsed dendritic cells in mice bearing breast cancer","volume":"63","author":"Kim","year":"2025","journal-title":"Parasites Hosts. Dis."},{"key":"10.1016\/j.exppara.2026.109209_bib19","article-title":"Immune responses in piglets infected with T. gondii","volume":"13","author":"Largo-de la Torre","year":"2022","journal-title":"Front. Immunol."},{"key":"10.1016\/j.exppara.2026.109209_bib20","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1186\/s13567-025-01645-2","article-title":"Inactivated vaccine against Toxoplasma gondii in pigs","volume":"56","author":"Largo-de la Torre","year":"2025","journal-title":"Vet. Res."},{"key":"10.1016\/j.exppara.2026.109209_bib21","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1007\/s11845-022-02998-9","article-title":"Vaccine candidates for Toxoplasma gondii: a review","volume":"192","author":"Mamaghani","year":"2023","journal-title":"Ir. J. Med. Sci."},{"key":"10.1016\/j.exppara.2026.109209_bib22","article-title":"DNA vaccine evaluation in cats","volume":"54","author":"Minutti","year":"2025","journal-title":"Vaccine"},{"key":"10.1016\/j.exppara.2026.109209_bib23","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1007\/s00436-019-06274-1","article-title":"Genotyping of Toxoplasma gondii in pigs","volume":"118","author":"Miura","year":"2019","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.exppara.2026.109209_bib24","doi-asserted-by":"crossref","first-page":"2223","DOI":"10.3389\/fimmu.2019.02223","article-title":"QuilA-adjuvanted vaccine responses in pigs","volume":"10","author":"Rahman","year":"2019","journal-title":"Front. Immunol."},{"key":"10.1016\/j.exppara.2026.109209_bib25","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1001\/jama.1941.02820090001001","article-title":"Toxoplasmic encephalitis in children","volume":"116","author":"Sabin","year":"1941","journal-title":"JAMA"},{"key":"10.1016\/j.exppara.2026.109209_bib26","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1186\/1756-3305-4-213","article-title":"DNA vaccination induces protection in mice","volume":"4","author":"Sun","year":"2011","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.exppara.2026.109209_bib27","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.vetpar.2011.02.015","article-title":"IFN-\u03b3 expression in infected pigs","volume":"179","author":"Verhelst","year":"2011","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.exppara.2026.109209_bib28","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1089\/mab.2019.0041","article-title":"Monoclonal antibody production against SAG1","volume":"39","author":"Vishteh","year":"2020","journal-title":"Monoclon. Antibodies Immunodiagn. Immunother."},{"key":"10.1016\/j.exppara.2026.109209_bib29","first-page":"3577","article-title":"Polyclonal antibody production against SAG1","volume":"45","author":"Vishteh","year":"2012","journal-title":"Biol. Res."},{"key":"10.1016\/j.exppara.2026.109209_bib30","doi-asserted-by":"crossref","first-page":"25","DOI":"10.3390\/pathogens7010025","article-title":"Congenital toxoplasmosis","volume":"7","author":"Wallon","year":"2018","journal-title":"Pathogens"},{"key":"10.1016\/j.exppara.2026.109209_bib31","doi-asserted-by":"crossref","first-page":"1387","DOI":"10.1007\/s11686-021-00415-2","article-title":"Combined DNA vaccine against T. gondii","volume":"66","author":"Wu","year":"2021","journal-title":"Acta Parasitol."},{"key":"10.1016\/j.exppara.2026.109209_bib32","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.exppara.2012.04.006","article-title":"Oocyst shedding in vaccinated cats","volume":"131","author":"Zulpo","year":"2012","journal-title":"Exp. Parasitol."}],"container-title":["Experimental Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489426001165?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489426001165?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T22:25:04Z","timestamp":1791325504000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014489426001165"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":32,"alternative-id":["S0014489426001165"],"URL":"https:\/\/doi.org\/10.1016\/j.exppara.2026.109209","relation":{},"ISSN":["0014-4894"],"issn-type":[{"value":"0014-4894","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Evaluation of tissue cyst protection in pigs immunized by intradermal and intramuscular routes with a DNA vaccine (rop18 + sag1) against Toxoplasma gondii","name":"articletitle","label":"Article Title"},{"value":"Experimental Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.exppara.2026.109209","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"109209"}}