{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T01:13:44Z","timestamp":1784250824814,"version":"3.55.0"},"reference-count":25,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,5,7]],"date-time":"2022-05-07T00:00:00Z","timestamp":1651881600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Food and Waterborne Parasitology"],"published-print":{"date-parts":[[2022,6]]},"DOI":"10.1016\/j.fawpar.2022.e00163","type":"journal-article","created":{"date-parts":[[2022,5,13]],"date-time":"2022-05-13T04:02:45Z","timestamp":1652414565000},"page":"e00163","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["A Simple Alcohol-based Method of Oocyst Inactivation for Use in the Development of Detection Assays for Cryptosporidium"],"prefix":"10.1016","volume":"27","author":[{"given":"Biniam","family":"Hagos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert E.","family":"Molestina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fawpar.2022.e00163_bb0005","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.actatropica.2018.01.011","article-title":"Methods for the detection of Cryptosporidium and Giardia: from microscopy to nucleic acid based tools in clinical and environmental regimes","volume":"184","author":"Adeyemo","year":"2018","journal-title":"Acta Trop."},{"key":"10.1016\/j.fawpar.2022.e00163_bb0010","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0216040","article-title":"Efficiency of chlorine and UV in the inactivation of Cryptosporidium and Giardia in wastewater","volume":"14","author":"Adeyemo","year":"2019","journal-title":"PLoS One"},{"key":"10.1016\/j.fawpar.2022.e00163_bb0015","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0051232","article-title":"Cryptosporidium parvum infection in SCID mice infected with only one oocyst: qPCR assessment of parasite replication in tissues and development of digestive cancer","volume":"7","author":"Benamrouz","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.fawpar.2022.e00163_bb0020","doi-asserted-by":"crossref","first-page":"4737","DOI":"10.1016\/S0043-1354(03)00435-4","article-title":"Synergistic inactivation of Cryptosporidium parvum using ozone followed by free chlorine in natural water","volume":"37","author":"Biswas","year":"2003","journal-title":"Water Res."},{"key":"10.1016\/j.fawpar.2022.e00163_bb0025","doi-asserted-by":"crossref","first-page":"2732","DOI":"10.1128\/aem.60.8.2732-2735.1994","article-title":"Effect of high temperature on infectivity of Cryptosporidium parvum oocysts in water","volume":"60","author":"Fayer","year":"1994","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.fawpar.2022.e00163_bb0030","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.mimet.2010.01.017","volume":"80","author":"Garvey","year":"2010","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.fawpar.2022.e00163_bb0035","doi-asserted-by":"crossref","first-page":"R193","DOI":"10.1016\/j.cub.2017.11.070","article-title":"Cryptosporidium","volume":"28","author":"Gibson","year":"2018","journal-title":"Curr. Biol."},{"key":"10.1016\/j.fawpar.2022.e00163_bb0040","doi-asserted-by":"crossref","first-page":"713","DOI":"10.4269\/ajtmh.2007.76.713","article-title":"Multiplex real-time PCR assay for detection of Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium spp","volume":"76","author":"Haque","year":"2007","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.fawpar.2022.e00163_bb0045","doi-asserted-by":"crossref","first-page":"2505","DOI":"10.1128\/AEM.69.5.2505-2511.2003","article-title":"Cell culture-Taqman PCR assay for evaluation of Cryptosporidium parvum disinfection","volume":"69","author":"Keegan","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.fawpar.2022.e00163_bb0050","doi-asserted-by":"crossref","first-page":"e758","DOI":"10.1016\/S2214-109X(18)30283-3","article-title":"Morbidity, mortality, and long-term consequences associated with diarrhoea from Cryptosporidium infection in children younger than 5 years: a meta-analyses study","volume":"6","author":"Khalil","year":"2018","journal-title":"Lancet Glob. Health"},{"key":"10.1016\/j.fawpar.2022.e00163_bb0055","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1017\/S0031182011000217","article-title":"Cryptosporidium cell culture infectivity assay design","volume":"138","author":"King","year":"2011","journal-title":"Parasitology"},{"key":"10.1016\/j.fawpar.2022.e00163_bb0060","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.mimet.2012.11.015","article-title":"Quantification of Cryptosporidium parvum in natural soil matrices and soil solutions using qPCR","volume":"92","author":"Koken","year":"2013","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.fawpar.2022.e00163_bb0065","series-title":"Cryptosporidium: Parasite and Disease","first-page":"515","article-title":"Removal and inactivation of Cryptosporidium from water","author":"Monis","year":"2014"},{"key":"10.1016\/j.fawpar.2022.e00163_bb0070","article-title":"Cryptosporidium spp. diagnosis and research in the 21(st) century","volume":"24","author":"O'Leary","year":"2021","journal-title":"Food Waterborne Parasitol."},{"key":"10.1016\/j.fawpar.2022.e00163_bb0075","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1111\/jam.14881","article-title":"Inactivation of Cryptosporidium parvum oocysts and faecal indicator bacteria in cattle slurry by addition of ammonia","volume":"130","author":"Petersen","year":"2021","journal-title":"J. Appl. Microbiol."},{"key":"10.1016\/j.fawpar.2022.e00163_bb0080","article-title":"Cryptosporidium: host-parasite interactions and pathogenesis","volume":"1-6","author":"Pinto","year":"2021","journal-title":"Curr. Clin. Microbiol. Rep."},{"key":"10.1016\/j.fawpar.2022.e00163_bb0085","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1046\/j.1472-765X.1999.00654.x","article-title":"In situ reverse transcription for the specific detection of bacteria and protozoa","volume":"29","author":"Prescott","year":"1999","journal-title":"Lett. Appl. Microbiol."},{"key":"10.1016\/j.fawpar.2022.e00163_bb0090","doi-asserted-by":"crossref","first-page":"2479","DOI":"10.1128\/AEM.71.5.2479-2483.2005","article-title":"Efficacy of two peroxygen-based disinfectants for inactivation of Cryptosporidium parvum oocysts","volume":"71","author":"Quilez","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.fawpar.2022.e00163_bb0095","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.vetpar.2009.03.009","article-title":"Combination of cell culture and quantitative PCR for screening of drugs against Cryptosporidium parvum","volume":"162","author":"Shahiduzzaman","year":"2009","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.fawpar.2022.e00163_bb0100","doi-asserted-by":"crossref","first-page":"3669","DOI":"10.1128\/aem.63.9.3669-3675.1997","article-title":"An in vitro method for detecting infectious Cryptosporidium oocysts with cell culture","volume":"63","author":"Slifko","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.fawpar.2022.e00163_bb0105","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0066562","article-title":"The applicability of TaqMan-based quantitative real-time PCR assays for detecting and enumerating Cryptosporidium spp. oocysts in the environment","volume":"8","author":"Staggs","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.fawpar.2022.e00163_bb0110","doi-asserted-by":"crossref","first-page":"1598","DOI":"10.1128\/aem.63.4.1598-1601.1997","article-title":"Inactivation of Cryptosporidium parvum oocysts and Clostridium perfringens spores by a mixed-oxidant disinfectant and by free chlorine","volume":"63","author":"Venczel","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.fawpar.2022.e00163_bb0115","doi-asserted-by":"crossref","first-page":"2576","DOI":"10.1128\/AEM.68.5.2576-2579.2002","article-title":"Efficacy of common laboratory disinfectants on the infectivity of Cryptosporidium parvum oocysts in cell culture","volume":"68","author":"Weir","year":"2002","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.fawpar.2022.e00163_bb0120","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1186\/s13071-019-3851-7","article-title":"Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR","volume":"12","author":"Woolsey","year":"2019","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.fawpar.2022.e00163_bb0125","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1016\/j.ijpara.2014.08.004","article-title":"Comparison of next-generation droplet digital PCR (ddPCR) with quantitative PCR (qPCR) for enumeration of Cryptosporidium oocysts in faecal samples","volume":"44","author":"Yang","year":"2014","journal-title":"Int. J. Parasitol."}],"container-title":["Food and Waterborne Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405676622000208?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405676622000208?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T08:44:10Z","timestamp":1762505050000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2405676622000208"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6]]},"references-count":25,"alternative-id":["S2405676622000208"],"URL":"https:\/\/doi.org\/10.1016\/j.fawpar.2022.e00163","relation":{},"ISSN":["2405-6766"],"issn-type":[{"value":"2405-6766","type":"print"}],"subject":[],"published":{"date-parts":[[2022,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A Simple Alcohol-based Method of Oocyst Inactivation for Use in the Development of Detection Assays for Cryptosporidium","name":"articletitle","label":"Article Title"},{"value":"Food and Waterborne Parasitology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fawpar.2022.e00163","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Authors. Published by Elsevier Inc. on behalf of International Association of Food and Waterborne Parasitology.","name":"copyright","label":"Copyright"}],"article-number":"e00163"}}