{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T18:41:38Z","timestamp":1792089698708,"version":"4.4.0"},"reference-count":52,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,8,12]],"date-time":"2022-08-12T00:00:00Z","timestamp":1660262400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Food and Chemical Toxicology"],"published-print":{"date-parts":[[2022,10]]},"DOI":"10.1016\/j.fct.2022.113329","type":"journal-article","created":{"date-parts":[[2022,8,7]],"date-time":"2022-08-07T18:26:02Z","timestamp":1659896762000},"page":"113329","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"special_numbering":"C","title":["Food safety assessment and toxicity study of the synbiotic consortium SBD111"],"prefix":"10.1016","volume":"168","author":[{"suffix":"Jr.","given":"D. Davidson","family":"Easson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2203-9921","authenticated-orcid":false,"given":"Vincent A.","family":"Murphy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicia E.","family":"Ballok","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria J.","family":"Soto-Giron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric M.","family":"Schott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7752-9880","authenticated-orcid":false,"given":"Joseph","family":"Rodricks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8033-2149","authenticated-orcid":false,"given":"Gerardo V.","family":"Toledo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fct.2022.113329_bib1","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/j.foodres.2015.09.016","article-title":"New insights in antibiotic resistance of Lactobacillus species from fermented foods","volume":"78","author":"Abriouel","year":"2015","journal-title":"Food Res. Int."},{"key":"10.1016\/j.fct.2022.113329_bib2","series-title":"Principles of Plant\u2013Microbe Interactions: Microbes for Sustainable Agriculture","first-page":"419","article-title":"The edible plant microbiome: importance and health issues","author":"Berg","year":"2015"},{"issue":"6275","key":"10.1016\/j.fct.2022.113329_bib3","doi-asserted-by":"crossref","DOI":"10.1126\/science.aad3311","article-title":"Gut bacteria that prevent growth impairments transmitted by microbiota from malnourished children","volume":"351","author":"Blanton","year":"2016","journal-title":"Science"},{"issue":"11","key":"10.1016\/j.fct.2022.113329_bib4","doi-asserted-by":"crossref","first-page":"1822","DOI":"10.1002\/jcp.24636","article-title":"Probiotic L. reuteri treatment prevents bone loss in a menopausal ovariectomized mouse model","volume":"229","author":"Britton","year":"2014","journal-title":"J. Cell. Physiol."},{"issue":"1","key":"10.1016\/j.fct.2022.113329_bib5","article-title":"BRAKER2: automatic eukaryotic genome annotation with GeneMark-EP+ and AUGUSTUS supported by a protein database","volume":"3","author":"Br\u016fna","year":"2021","journal-title":"Nucleic Acids Res. Genom. Bioinf."},{"issue":"7","key":"10.1016\/j.fct.2022.113329_bib6","doi-asserted-by":"crossref","first-page":"1761","DOI":"10.2337\/db06-1491","article-title":"Metabolic endotoxemia initiates obesity and insulin resistance","volume":"56","author":"Cani","year":"2007","journal-title":"Diabetes"},{"issue":"4","key":"10.1016\/j.fct.2022.113329_bib7","doi-asserted-by":"crossref","first-page":"e73","DOI":"10.1038\/cti.2016.17","article-title":"Regulation of immune cell function by short-chain fatty acids","volume":"5","author":"Corr\u00eaa-Oliveira","year":"2016","journal-title":"Transl. Clin. Immunol."},{"key":"10.1016\/j.fct.2022.113329_bib8","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1186\/1471-2105-11-485","article-title":"SolexaQA: at-a-glance quality assessment of Illumina second-generation sequencing data","volume":"11","author":"Cox","year":"2010","journal-title":"BMC Bioinf."},{"key":"10.1016\/j.fct.2022.113329_bib9","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.nut.2018.02.013","article-title":"Bacillus clausii inhibits bone loss by skewing Treg-Th17 cell equilibrium in postmenopausal osteoporotic mice model","volume":"54","author":"Dar","year":"2018","journal-title":"Nutrition"},{"key":"10.1016\/j.fct.2022.113329_bib10","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.bonr.2018.02.001","article-title":"Lactobacillus acidophilus inhibits bone loss and increases bone heterogeneity in osteoporotic mice via modulating Treg-Th17 cell balance","volume":"8","author":"Dar","year":"2018","journal-title":"BoneKEy Rep."},{"issue":"15","key":"10.1016\/j.fct.2022.113329_bib11","doi-asserted-by":"crossref","first-page":"2666","DOI":"10.1093\/bioinformatics\/bty149","article-title":"NanoPack: visualizing and processing long-read sequencing data","volume":"34","author":"De Coster","year":"2018","journal-title":"Bioinformatics"},{"issue":"6","key":"10.1016\/j.fct.2022.113329_bib12","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1093\/mmy\/myz121","article-title":"Susceptibility of uncommon Candida species to systemic antifungals by the EUCAST methodology","volume":"58","author":"D\u00edaz-Garc\u00eda","year":"2019","journal-title":"Med. Mycol."},{"issue":"7","key":"10.1016\/j.fct.2022.113329_bib13","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1007138","article-title":"Population genomics shows no distinction between pathogenic Candida krusei and environmental Pichia kudriavzevii: one species, four names","volume":"14","author":"Douglass","year":"2018","journal-title":"PLoS Pathog."},{"issue":"1","key":"10.1016\/j.fct.2022.113329_bib14","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0145203","article-title":"Antibiotic susceptibility profiles of dairy leuconostoc, analysis of the genetic basis of atypical resistances and transfer of genes in vitro and in a food matrix","volume":"11","author":"Fl\u00f3rez","year":"2016","journal-title":"PLoS One"},{"issue":"9","key":"10.1016\/j.fct.2022.113329_bib15","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.4315\/0362-028X-72.9.1923","article-title":"Assessment of antibiotic resistance in probiotic strain Lactobacillus brevis KB290","volume":"72","author":"Fukao","year":"2009","journal-title":"J. Food Protect."},{"issue":"5","key":"10.1016\/j.fct.2022.113329_bib16","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1016\/j.chom.2016.04.017","article-title":"Genetic determinants of the gut microbiome in UK twins","volume":"19","author":"Goodrich","year":"2016","journal-title":"Cell Host Microbe"},{"issue":"8","key":"10.1016\/j.fct.2022.113329_bib17","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1093\/bioinformatics\/btt086","article-title":"QUAST: quality assessment tool for genome assemblies","volume":"29","author":"Gurevich","year":"2013","journal-title":"Bioinformatics"},{"key":"10.1016\/j.fct.2022.113329_bib18","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1186\/1471-2105-11-119","article-title":"Prodigal: prokaryotic gene recognition and translation initiation site identification","volume":"11","author":"Hyatt","year":"2010","journal-title":"BMC Bioinf."},{"issue":"3","key":"10.1016\/j.fct.2022.113329_bib19","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/S2665-9913(19)30068-2","article-title":"Probiotic treatment using a mix of three Lactobacillus strains for lumbar spine bone loss in postmenopausal women: a randomized, double-blind, placebo-controlled, multicentre trial","volume":"1","author":"Jansson","year":"2019","journal-title":"Lancet Rheumatol."},{"issue":"7351","key":"10.1016\/j.fct.2022.113329_bib21","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1038\/nature10213","article-title":"Human nutrition, the gut microbiome and the immune system","volume":"474","author":"Kau","year":"2011","journal-title":"Nature"},{"issue":"5","key":"10.1016\/j.fct.2022.113329_bib22","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1038\/s41587-019-0072-8","article-title":"Assembly of long, error-prone reads using repeat graphs","volume":"37","author":"Kolmogorov","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.fct.2022.113329_bib23","first-page":"503","article-title":"Emendation of the genus Issatchenkia Kudriavzev and comparison of species by deoxyribonucleic acid reassociation, mating reaction, and ascospore ultrastructure","volume":"30","author":"Kurtzman","year":"1980","journal-title":"Int. J. Syst. Evol. Microbiol."},{"issue":"3","key":"10.1016\/j.fct.2022.113329_bib24","doi-asserted-by":"crossref","first-page":"909","DOI":"10.3945\/ajcn.117.153353","article-title":"Combined bioavailable isoflavones and probiotics improve bone status and estrogen metabolism in postmenopausal osteopenic women: a randomized controlled trial","volume":"106","author":"Lambert","year":"2017","journal-title":"Am. J. Clin. Nutr."},{"issue":"4","key":"10.1016\/j.fct.2022.113329_bib25","doi-asserted-by":"crossref","first-page":"E344","DOI":"10.1152\/ajpendo.00366.2021","article-title":"Development of a synbiotic that protects against ovariectomy-induced trabecular bone loss","volume":"322","author":"Lawenius","year":"2022","journal-title":"Am. J. Physiol. Endocrinol. Metabol."},{"issue":"6","key":"10.1016\/j.fct.2022.113329_bib26","doi-asserted-by":"crossref","first-page":"2049","DOI":"10.1172\/JCI86062","article-title":"Sex steroid deficiency\u2013associated bone loss is microbiota dependent and prevented by probiotics","volume":"126","author":"Li","year":"2016","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.fct.2022.113329_bib27","doi-asserted-by":"crossref","DOI":"10.1590\/1981-6723.19718","article-title":"Yeasts isolated from tropical fruit ice creams: diversity, antifungal susceptibility and adherence to buccal epithelial cells","volume":"22","author":"Lima","year":"2019","journal-title":"Braz. J. Food Technol."},{"issue":"5","key":"10.1016\/j.fct.2022.113329_bib28","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/S0895-3988(10)60018-9","article-title":"Antibiotic resistance of probiotic strains of lactic acid bacteria isolated from marketed foods and drugs","volume":"22","author":"Liu","year":"2009","journal-title":"Biomed. Environ. Sci."},{"issue":"1","key":"10.1016\/j.fct.2022.113329_bib29","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1038\/s41467-017-02490-4","article-title":"Short-chain fatty acids regulate systemic bone mass and protect from pathological bone loss","volume":"9","author":"Lucas","year":"2018","journal-title":"Nat. Commun."},{"issue":"6297","key":"10.1016\/j.fct.2022.113329_bib30","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1126\/science.aaf3951","article-title":"Cospeciation of gut microbiota with hominids","volume":"353","author":"Moeller","year":"2016","journal-title":"Science"},{"issue":"3","key":"10.1016\/j.fct.2022.113329_bib32","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1111\/joim.12805","article-title":"Lactobacillus reuteri reduces bone loss in older women with low bone mineral density: a randomized, placebo-controlled, double-blind, clinical trial","volume":"284","author":"Nilsson","year":"2018","journal-title":"J. Intern. Med."},{"key":"10.1016\/j.fct.2022.113329_bib33","series-title":"Guide for the Care and Use of Laboratory Animals. Committee for the Update of the Guide for the Care and Use of Laboratory Animals","year":"2011"},{"issue":"3","key":"10.1016\/j.fct.2022.113329_bib34","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0092368","article-title":"Probiotics protect mice from ovariectomy-induced cortical bone loss","volume":"9","author":"Ohlsson","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.fct.2022.113329_bib35","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.coph.2018.03.007","article-title":"Acquired and congenital forms of heterotopic ossification: new pathogenic insights and therapeutic opportunities","volume":"40","author":"Pacifici","year":"2018","journal-title":"Curr. Opin. Pharmacol."},{"issue":"7","key":"10.1016\/j.fct.2022.113329_bib36","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1101\/gr.186072.114","article-title":"CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes","volume":"25","author":"Parks","year":"2015","journal-title":"Genome Res."},{"key":"10.1016\/j.fct.2022.113329_bib37","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.1093\/bioinformatics\/bts174","article-title":"IDBA-UD: a de novo assembler for single-cell and metagenomic sequencing data with highly uneven depth","volume":"28","author":"Peng","year":"2012","journal-title":"Bioinformatics"},{"key":"10.1016\/j.fct.2022.113329_bib38","series-title":"Medical Microbiology","article-title":"Bacterial pathogenesis","author":"Peterson","year":"1996"},{"issue":"6","key":"10.1016\/j.fct.2022.113329_bib39","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1016\/j.cell.2016.11.018","article-title":"Gut microbiota regulate motor deficits and neuroinflammation in a model of Parkinson's Disease","volume":"167","author":"Sampson","year":"2016","journal-title":"Cell"},{"issue":"14","key":"10.1016\/j.fct.2022.113329_bib40","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: rapid prokaryotic genome annotation","volume":"30","author":"Seemann","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.fct.2022.113329_bib41","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/978-1-4939-9173-0_14","article-title":"BUSCO: assessing genome assembly and annotation completeness","volume":"1962","author":"Seppey","year":"2019","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.fct.2022.113329_bib42","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.foodcont.2014.09.003","article-title":"Comparative studies on antibiotic resistance in Lactobacillus casei and Lactobacillus plantarum","volume":"50","author":"Shao","year":"2015","journal-title":"Food Control"},{"issue":"1","key":"10.1016\/j.fct.2022.113329_bib43","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-03334-4","article-title":"The Edible Plant Microbiome represents a diverse genetic reservoir with functional potential in the human host","volume":"11","author":"Soto-Giron","year":"2021","journal-title":"Sci. Rep."},{"issue":"Suppl. 2","key":"10.1016\/j.fct.2022.113329_bib44","doi-asserted-by":"crossref","first-page":"W435","DOI":"10.1093\/nar\/gkl200","article-title":"AUGUSTUS: ab initio prediction of alternative transcripts","volume":"34","author":"Stanke","year":"2006","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.fct.2022.113329_bib47","doi-asserted-by":"crossref","first-page":"1979","DOI":"10.1101\/gr.081612.108","article-title":"Gene prediction in novel fungal genomes using an ab initio algorithm with unsupervised training","volume":"18","author":"Ter-Hovhannisyan","year":"2008","journal-title":"Genome Res."},{"issue":"2","key":"10.1016\/j.fct.2022.113329_bib48","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1038\/nm.3444","article-title":"Gut microbiota metabolism of dietary fiber influences allergic airway disease and hematopoiesis","volume":"20","author":"Trompette","year":"2014","journal-title":"Nat. Med."},{"issue":"7122","key":"10.1016\/j.fct.2022.113329_bib49","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1038\/nature05414","article-title":"An obesity-associated gut microbiome with increased capacity for energy harvest","volume":"444","author":"Turnbaugh","year":"2006","journal-title":"Nature"},{"key":"10.1016\/j.fct.2022.113329_bib45","doi-asserted-by":"crossref","first-page":"190","DOI":"10.3389\/fmicb.2011.00190","article-title":"Human health and disease in a microbial world","volume":"2","author":"Turnbaugh","year":"2011","journal-title":"Front. Microbiol."},{"issue":"6","key":"10.1016\/j.fct.2022.113329_bib50","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.1016\/j.immuni.2018.10.013","article-title":"The microbial metabolite butyrate stimulates bone formation via t regulatory cell-mediated regulation of WNT10B expression","volume":"49","author":"Tyagi","year":"2018","journal-title":"Immunity"},{"key":"10.1016\/j.fct.2022.113329_bib51","series-title":"Toxicological Principles for the Safety Assessment of Food Ingredients. Redbook 2000 Chapter IV.C.3.a. Short-Term Toxicity Studies with Rodents. United States Food and Drug Administration","first-page":"2003","year":"2003"},{"key":"10.1016\/j.fct.2022.113329_bib52","series-title":"Guidance for Industry: Estimating the Maximum Safe Starting Dose in Initial Clinical Trials for Therapeutics in Adult Healthy Volunteers. United States Department of Health and Human Services","year":"2005"},{"issue":"11","key":"10.1016\/j.fct.2022.113329_bib53","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0112963","article-title":"Pilon: an integrated tool for comprehensive microbial variant detection and genome assembly improvement","volume":"9","author":"Walker","year":"2014","journal-title":"PLoS One"},{"issue":"1","key":"10.1016\/j.fct.2022.113329_bib54","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-6968.2001.tb10724.x","article-title":"Bacterial virulence: can we draw the line?","volume":"201","author":"Wassenaar","year":"2001","journal-title":"FEMS (Fed. Eur. Microbiol. Soc.) Microbiol. Lett."},{"issue":"12","key":"10.1016\/j.fct.2022.113329_bib55","doi-asserted-by":"crossref","first-page":"4104","DOI":"10.1002\/art.23138","article-title":"Treg cells suppress osteoclast formation: a new link between the immune system and bone","volume":"56","author":"Zaiss","year":"2007","journal-title":"Arthritis Rheum."}],"container-title":["Food and Chemical Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0278691522005270?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0278691522005270?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T22:21:27Z","timestamp":1759530087000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0278691522005270"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10]]},"references-count":52,"alternative-id":["S0278691522005270"],"URL":"https:\/\/doi.org\/10.1016\/j.fct.2022.113329","relation":{},"ISSN":["0278-6915"],"issn-type":[{"value":"0278-6915","type":"print"}],"subject":[],"published":{"date-parts":[[2022,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Food safety assessment and toxicity study of the synbiotic consortium SBD111","name":"articletitle","label":"Article Title"},{"value":"Food and Chemical Toxicology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fct.2022.113329","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"113329"}}