{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T06:46:13Z","timestamp":1792565173126,"version":"4.5.0"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100017962","name":"Jiangsu Commission of Health","doi-asserted-by":"publisher","award":["Z2022027"],"award-info":[{"award-number":["Z2022027"]}],"id":[{"id":"10.13039\/100017962","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Food and Chemical Toxicology"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.fct.2026.116427","type":"journal-article","created":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T22:30:18Z","timestamp":1791066618000},"page":"116427","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["5-Hydroxymethylfurfural induces skeletal toxicity associated with ROS accumulation by disrupting cartilage and bone remodeling in zebrafish larvae"],"prefix":"10.1016","volume":"218","author":[{"given":"Meiling","family":"Lai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fct.2026.116427_bib1","doi-asserted-by":"crossref","first-page":"3576","DOI":"10.3390\/ijms20143576","article-title":"Reactive oxygen species in osteoclast differentiation and possible pharmaceutical targets of ROS-mediated osteoclast diseases","volume":"20","author":"Agidigbi","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.fct.2026.116427_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.cbi.2025.111886","article-title":"5-Hydroxymethylfurfural triggers autophagy and apoptosis in sertoli and spermatogonial cells via suppression of the PI3K\/AKT\/mTOR pathway","volume":"424","author":"Aydin","year":"2026","journal-title":"Chem. Biol. Interact."},{"key":"10.1016\/j.fct.2026.116427_bib3","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1080\/15376516.2025.2537319","article-title":"5-Hydroxymethylfurfural causes reproductive toxicity in male mice by increasing oxidative stress and apoptosis through the Nrf2\/HO-1 signaling pathway","volume":"35","author":"Aydin","year":"2025","journal-title":"Toxicol. Mech. Methods"},{"key":"10.1016\/j.fct.2026.116427_bib4","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1038\/s41423-022-00959-x","article-title":"RANKL-responsive epigenetic mechanism reprograms macrophages into bone-resorbing osteoclasts","volume":"20","author":"Bae","year":"2023","journal-title":"Cell. Mol. Immunol."},{"key":"10.1016\/j.fct.2026.116427_bib5","doi-asserted-by":"crossref","DOI":"10.1126\/science.abc1048","article-title":"Developmental chromatin programs determine oncogenic competence in melanoma","volume":"373","author":"Baggiolini","year":"2021","journal-title":"Science"},{"key":"10.1016\/j.fct.2026.116427_bib6","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.bone.2015.04.035","article-title":"Bone development","volume":"80","author":"Berendsen","year":"2015","journal-title":"Bone"},{"key":"10.1016\/j.fct.2026.116427_bib7","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.jphs.2020.06.001","article-title":"Liquiritigenin reduces osteoclast activity in zebrafish model of glucocorticoid-induced osteoporosis","volume":"143","author":"Carnovali","year":"2020","journal-title":"J. Pharmacol. Sci."},{"key":"10.1016\/j.fct.2026.116427_bib8","doi-asserted-by":"crossref","DOI":"10.1016\/j.fct.2024.114738","article-title":"5-Hydroxymethylfurfural mediated developmental toxicity in Drosophila melanogaster","volume":"189","author":"Chen","year":"2024","journal-title":"Food Chem. Toxicol."},{"key":"10.1016\/j.fct.2026.116427_bib9","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1038\/s12276-021-00571-5","article-title":"Zebrafish as an animal model for biomedical research","volume":"53","author":"Choi","year":"2021","journal-title":"Exp. Mol. Med."},{"key":"10.1016\/j.fct.2026.116427_bib10","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1002\/dvg.20639","article-title":"Zebrafish sp7:EGFP: a transgenic for studying otic vesicle formation, skeletogenesis, and bone regeneration","volume":"48","author":"DeLaurier","year":"2010","journal-title":"Genesis"},{"key":"10.1016\/j.fct.2026.116427_bib11","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1002\/jbmr.4256","article-title":"Skeletal biology and disease modeling in zebrafish","volume":"36","author":"Dietrich","year":"2020","journal-title":"J. Bone Miner. Res."},{"key":"10.1016\/j.fct.2026.116427_bib12","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1089\/zeb.2009.0641","article-title":"A quick whole-mount staining protocol for bone deposition and resorption","volume":"7","author":"Edsall","year":"2010","journal-title":"Zebrafish"},{"key":"10.1016\/j.fct.2026.116427_bib13","doi-asserted-by":"crossref","first-page":"1941","DOI":"10.3390\/molecules25081941","article-title":"The toxicological aspects of the heat-borne toxicant 5-Hydroxymethylfurfural in animals: a review","volume":"25","author":"Farag","year":"2020","journal-title":"Molecules"},{"key":"10.1016\/j.fct.2026.116427_bib14","doi-asserted-by":"crossref","first-page":"6948","DOI":"10.1038\/s41467-024-50780-5","article-title":"Cellular transitions during cranial suture establishment in zebrafish","volume":"15","author":"Farmer","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.fct.2026.116427_bib15","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1146\/annurev-pathmechdis-051222-121126","article-title":"ENPP1 in blood and bone: skeletal and soft tissue diseases induced by ENPP1 deficiency","volume":"19","author":"Ferreira","year":"2024","journal-title":"Annu. Rev. Pathol."},{"key":"10.1016\/j.fct.2026.116427_bib16","unstructured":"Fish models of induced osteoporosis - PubMed, (n.d.). https:\/\/pubmed.ncbi.nlm.nih.gov\/34179000\/(accessed June 26, 2026)."},{"key":"10.1016\/j.fct.2026.116427_bib17","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1002\/mnfr.201100574","article-title":"Study of 5-hydroxymethylfurfural and its metabolite 5-sulfooxymethylfurfural on induction of colonic aberrant crypt foci in wild-type mice and transgenic mice expressing human sulfotransferases 1A1 and 1A2","volume":"56","author":"Florian","year":"2012","journal-title":"Mol. Nutr. Food Res."},{"key":"10.1016\/j.fct.2026.116427_bib18","doi-asserted-by":"crossref","DOI":"10.3791\/58429","article-title":"Adult zebrafish injury models to study the effects of prednisolone in regenerating bone tissue","author":"Geurtzen","year":"2018","journal-title":"J. Vis. Exp."},{"key":"10.1016\/j.fct.2026.116427_bib19","doi-asserted-by":"crossref","first-page":"480","DOI":"10.3390\/biom11030480","article-title":"Zebrafish mafbb mutants display osteoclast over-activation and bone deformity resembling osteolysis in MCTO patients","volume":"11","author":"Han","year":"2021","journal-title":"Biomolecules"},{"key":"10.1016\/j.fct.2026.116427_bib20","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1016\/j.biopha.2018.02.082","article-title":"Possible mechanisms of prednisolone-induced osteoporosis in zebrafish larva","volume":"101","author":"He","year":"2018","journal-title":"Biomed. Pharmacother."},{"key":"10.1016\/j.fct.2026.116427_bib21","doi-asserted-by":"crossref","first-page":"1814","DOI":"10.3390\/foods11121814","article-title":"Comparison of the effects of 5-Hydroxymethylfurfural in milk powder matrix and standard water on oxidative stress system of zebrafish","volume":"11","author":"Hou","year":"2022","journal-title":"Foods"},{"key":"10.1016\/j.fct.2026.116427_bib22","doi-asserted-by":"crossref","first-page":"16955","DOI":"10.1021\/acsomega.1c01894","article-title":"Adsorption of 5-Hydroxymethylfurfural, levulinic acid, formic acid, and glucose using polymeric resins modified with different functional groups","volume":"6","author":"Hu","year":"2021","journal-title":"ACS Omega"},{"key":"10.1016\/j.fct.2026.116427_bib23","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1038\/nbt.2501","article-title":"Efficient genome editing in zebrafish using a CRISPR-cas system","volume":"31","author":"Hwang","year":"2013","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.fct.2026.116427_bib24","doi-asserted-by":"crossref","first-page":"3772","DOI":"10.3390\/ijms24043772","article-title":"Oxidative stress and inflammation in osteoporosis: molecular mechanisms involved and the relationship with microRNAs","volume":"24","author":"Iantomasi","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.fct.2026.116427_bib25","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.taap.2018.09.031","article-title":"Identification of 5-hydroxymethylfurfural in cigarette smoke extract as a new substrate metabolically activated by human cytochrome P450 2A13","volume":"359","author":"Ji","year":"2018","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.fct.2026.116427_bib26","doi-asserted-by":"crossref","DOI":"10.1016\/j.cbpc.2022.109452","article-title":"5-HMF affects cardiovascular development in zebrafish larvae via reactive oxygen species and Wnt signaling pathways","volume":"262","author":"Jiang","year":"2022","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"key":"10.1016\/j.fct.2026.116427_bib28","doi-asserted-by":"crossref","DOI":"10.1016\/j.cbpc.2022.109452","article-title":"5-HMF affects cardiovascular development in zebrafish larvae via reactive oxygen species and Wnt signaling pathways","volume":"262","author":"Jiang","year":"2022","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"key":"10.1016\/j.fct.2026.116427_bib27","doi-asserted-by":"crossref","DOI":"10.1016\/j.cbpc.2021.109254","article-title":"5-Hydroxymethyl-2-furaldehyde induces developmental toxicology and decreases bone mineralization in zebrafish larvae","volume":"254","author":"Jiang","year":"2022","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"key":"10.1016\/j.fct.2026.116427_bib29","doi-asserted-by":"crossref","DOI":"10.3390\/cells9092073","article-title":"Osteoblast-osteoclast communication and bone homeostasis","volume":"9","author":"Kim","year":"2020","journal-title":"Cells"},{"key":"10.1016\/j.fct.2026.116427_bib30","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.bone.2019.02.009","article-title":"Using zebrafish to study skeletal genomics","volume":"126","author":"Kwon","year":"2019","journal-title":"Bone"},{"key":"10.1016\/j.fct.2026.116427_bib31","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1038\/s41586-023-06002-x","article-title":"Physiology and diseases of tissue-resident macrophages","volume":"618","author":"Lazarov","year":"2023","journal-title":"Nature"},{"key":"10.1016\/j.fct.2026.116427_bib32","doi-asserted-by":"crossref","first-page":"4344","DOI":"10.1038\/s41467-025-59725-y","article-title":"Streamlined metal-based hydrogel facilitates stem cell differentiation, extracellular matrix homeostasis and cartilage repair in male rats","volume":"16","author":"Li","year":"2025","journal-title":"Nat. Commun."},{"key":"10.1016\/j.fct.2026.116427_bib33","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1038\/s41413-025-00497-y","article-title":"TGF-\u03b2\/BMP signaling in skeletal biology: molecular mechanisms, regulatory networks, and therapeutic implications in development, regeneration, and disease","volume":"14","author":"Liao","year":"2026","journal-title":"Bone Res."},{"key":"10.1016\/j.fct.2026.116427_bib34","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.aaf.2019.12.009","article-title":"Sox9a, not sox9b is required for normal cartilage development in zebrafish","volume":"6","author":"Lin","year":"2021","journal-title":"Aquac. Fish."},{"key":"10.1016\/j.fct.2026.116427_bib35","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a008334","article-title":"Development of the endochondral skeleton","volume":"5","author":"Long","year":"2013","journal-title":"Cold Spring Harbor Perspect. Biol."},{"key":"10.1016\/j.fct.2026.116427_bib36","doi-asserted-by":"crossref","first-page":"6027","DOI":"10.1021\/jf100649z","article-title":"Determination of furan precursors and some thermal damage markers in baby foods: ascorbic acid, dehydroascorbic acid, hydroxymethylfurfural and furfural","volume":"58","author":"Mes\u00edas-Garc\u00eda","year":"2010","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.fct.2026.116427_bib37","doi-asserted-by":"crossref","first-page":"3852","DOI":"10.1021\/acs.jafc.2c00269","article-title":"5-Hydroxymethylfurfural exerts negative effects on gastric mucosal epithelial cells by inducing oxidative stress, apoptosis, and tight junction disruption","volume":"70","author":"Qiu","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.fct.2026.116427_bib38","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2019.135097","article-title":"AHR-mediated ROS production contributes to the cardiac developmental toxicity of PM2.5 in zebrafish embryos","volume":"719","author":"Ren","year":"2020","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.fct.2026.116427_bib39","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1126\/science.1183090","article-title":"Zebrafish behavioral profiling links drugs to biological targets and rest\/wake regulation","volume":"327","author":"Rihel","year":"2010","journal-title":"Science"},{"key":"10.1016\/j.fct.2026.116427_bib40","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/s13065-018-0408-3","article-title":"5-Hydroxymethylfurfural (HMF) levels in honey and other food products: effects on bees and human health","volume":"12","author":"Shapla","year":"2018","journal-title":"Chem. Cent. J."},{"key":"10.1016\/j.fct.2026.116427_bib41","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1038\/s41586-024-08189-z","article-title":"A multi-omic atlas of human embryonic skeletal development","volume":"635","author":"To","year":"2024","journal-title":"Nature"},{"key":"10.1016\/j.fct.2026.116427_bib42","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/s00774-020-01162-6","article-title":"Osteoclast differentiation by RANKL and OPG signaling pathways","volume":"39","author":"Udagawa","year":"2021","journal-title":"J. Bone Miner. Metabol."},{"key":"10.1016\/j.fct.2026.116427_bib43","article-title":"Endothelial cell-derived lactate triggers bone mesenchymal stem cell histone lactylation to attenuate osteoporosis","volume":"10","author":"Wu","year":"2023","journal-title":"Adv. Sci. (Weinh.)"},{"key":"10.1016\/j.fct.2026.116427_bib44","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1038\/s41422-023-00918-9","article-title":"The roles and regulatory mechanisms of TGF-\u03b2 and BMP signaling in bone and cartilage development, homeostasis and disease","volume":"34","author":"Wu","year":"2024","journal-title":"Cell Res."},{"key":"10.1016\/j.fct.2026.116427_bib45","doi-asserted-by":"crossref","first-page":"220","DOI":"10.3390\/genes8090220","article-title":"Zebrafish xenograft: an evolutionary experiment in tumour biology","volume":"8","author":"Wyatt","year":"2017","journal-title":"Genes"},{"key":"10.1016\/j.fct.2026.116427_bib46","doi-asserted-by":"crossref","first-page":"2417","DOI":"10.1007\/s13197-019-03708-7","article-title":"Pathway of 5-hydroxymethyl-2-furaldehyde formation in honey","volume":"56","author":"Yang","year":"2019","journal-title":"J. Food Sci. Technol."},{"key":"10.1016\/j.fct.2026.116427_bib47","doi-asserted-by":"crossref","DOI":"10.1016\/j.biopha.2023.114834","article-title":"Oxidative stress: a common pathological state in a high-risk population for osteoporosis","volume":"163","author":"Zhang","year":"2023","journal-title":"Biomed. Pharmacother."},{"key":"10.1016\/j.fct.2026.116427_bib48","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1186\/s12967-024-05799-z","article-title":"From dysfunction to healing: advances in mitochondrial therapy for osteoarthritis","volume":"22","author":"Zhang","year":"2024","journal-title":"J. Transl. Med."},{"key":"10.1016\/j.fct.2026.116427_bib49","doi-asserted-by":"crossref","DOI":"10.1016\/j.envpol.2020.116395","article-title":"Exposure to acrylamide induces skeletal developmental toxicity in zebrafish and rat embryos","volume":"271","author":"Zhu","year":"2021","journal-title":"Environ. Pollut."}],"container-title":["Food and Chemical Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0278691526005028?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0278691526005028?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T06:19:33Z","timestamp":1792563573000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0278691526005028"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":49,"alternative-id":["S0278691526005028"],"URL":"https:\/\/doi.org\/10.1016\/j.fct.2026.116427","relation":{},"ISSN":["0278-6915"],"issn-type":[{"value":"0278-6915","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"5-Hydroxymethylfurfural induces skeletal toxicity associated with ROS accumulation by disrupting cartilage and bone remodeling in zebrafish larvae","name":"articletitle","label":"Article Title"},{"value":"Food and Chemical Toxicology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fct.2026.116427","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"116427"}}