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In addition to CLIC1, Western blot showed that two additional CLIC family members, CLIC4 and CLIC5, are also present in spermatozoa. CLIC fusion proteins, GST\u2010CLIC1, GST\u2010CLIC4 and GST\u2010CLIC5, were all able to bind to PP1\u03b32 in sperm extracts during pull\u2010down assays. Immunofluorescence microscopy revealed that each of the three isoforms occupies a distinct location within the cell. Given that PP1\u03b32 is a key enzyme regulating sperm motility, PP1\u03b32\u2010binding proteins, such as the CLIC proteins, are likely to play significant roles in sperm function.<\/jats:p>","DOI":"10.1016\/j.febslet.2004.04.017","type":"journal-article","created":{"date-parts":[[2004,4,24]],"date-time":"2004-04-24T09:18:16Z","timestamp":1082798296000},"page":"136-140","source":"Crossref","is-referenced-by-count":21,"title":["Identification of chloride intracellular channel proteins in spermatozoa"],"prefix":"10.1002","volume":"566","author":[{"given":"Kimberley","family":"Myers","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Payaningal R.","family":"Somanath","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Berryman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srinivasan","family":"Vijayaraghavan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,4,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD9960553"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/13.suppl_4.20"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1999.tb02495.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod54.3.709"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod54.3.719"},{"key":"e_1_2_6_7_1","unstructured":"Huang Z. 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