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This leads to actin polymerization through the effector, N\u2010WASP and the Arp2\/3 complex. Here, we have used reconstitution of vesicle budding to test whether Arp2\/3 is recruited to membranes during the formation of COPI vesicles. Our results revealed that ARF1 activation leads to greatly increased Arp3 levels on the membranes. Coatomer\u2010bound Cdc42 and pre\u2010existing F\u2010actin are important for Arp2\/3 binding. ARF1\u2010dependent Arp2\/3 recruitment and actin polymerization can be reconstituted on liposomal membranes, indicating that no membrane proteins are necessary. These results show that activated ARF1 can stimulate Arp2\/3 recruitment to Golgi membranes through coatomer, Cdc42 or Rac, and N\u2010WASP.<\/jats:p>","DOI":"10.1016\/j.febslet.2004.04.061","type":"journal-article","created":{"date-parts":[[2004,5,8]],"date-time":"2004-05-08T06:52:39Z","timestamp":1083999159000},"page":"281-286","source":"Crossref","is-referenced-by-count":47,"title":["Cytosol\u2010derived proteins are sufficient for Arp2\/3 recruitment and ARF\/coatomer\u2010dependent actin polymerization on Golgi 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