{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T13:47:25Z","timestamp":1792504045610,"version":"4.5.0"},"reference-count":19,"publisher":"Wiley","issue":"1-3","license":[{"start":{"date-parts":[[2004,7,4]],"date-time":"2004-07-04T00:00:00Z","timestamp":1088899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2004,7,30]]},"abstract":"<jats:p>Wheat gliadin is the triggering agent in coeliac disease. In this study, we documented that proteolytic fragments of gliadin, in contrast to other food antigens, induced interleukin (IL)\u20108 and tumour necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) production and significantly increased interferon (IFN)\u2010\u03b3\u2010induced cytokine secretion in human monocytic line THP\u20101 cells. Stimulation with gliadin resulted in elevated phosphorylation of the I\u03baB\u03b1 molecule and increased NF\u2010\u03baB\/DNA binding activity that was inhibited by sulfasalazine, <jats:sc>l<\/jats:sc>\u20101\u2010tosylamido\u20102\u2010phenylethyl chloromethyl ketone and pyrrolidine dithiocarbamate (PDTC). The activation pathway was shown to be independent of the CD14 molecule. Less mature U\u2010937 monocytes responded to gliadin stimulation by low IL\u20108 secretion, TNF\u2010\u03b1 production was not detectable. We propose that gliadin\u2010induced activation of monocytes\/macrophages can participate in mechanisms leading to the impairment of intestinal mucosa in coeliac patients.<\/jats:p>","DOI":"10.1016\/j.febslet.2004.06.057","type":"journal-article","created":{"date-parts":[[2004,7,7]],"date-time":"2004-07-07T15:49:56Z","timestamp":1089215396000},"page":"81-85","source":"Crossref","is-referenced-by-count":86,"title":["Gliadin stimulates human monocytes to production of IL\u20108 and TNF\u2010\u03b1 through a mechanism involving NF\u2010\u03baB"],"prefix":"10.1002","volume":"571","author":[{"given":"Lenka","family":"Jel\u0131\u0301nkov\u00e1","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludmila","family":"Tu\u010dkov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Cinov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuzana","family":"Flegelov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena","family":"Tlaskalov\u00e1-Hogenov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,7,4]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc883"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(98)70134-9"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.67.3.312"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.71.4.625"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.16.1.225"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)13803-2"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(03)01047-3"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-003-0771-y"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(03)00236-9"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)13843-3"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1074129"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.3.1174-1180.1998"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02041244"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(96)00073-7"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.4.1173-1180.1996"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI992"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-200204000-00011"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.14.1.649"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.febslet.2004.06.057","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.febslet.2004.06.057","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T16:52:03Z","timestamp":1697129523000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/j.febslet.2004.06.057"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,7,4]]},"references-count":19,"journal-issue":{"issue":"1-3","published-print":{"date-parts":[[2004,7,30]]}},"alternative-id":["10.1016\/j.febslet.2004.06.057"],"URL":"https:\/\/doi.org\/10.1016\/j.febslet.2004.06.057","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,7,4]]}}}