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The cDNA encoding batroxobin was cloned, expressed in <jats:italic>Pichia pastoris<\/jats:italic> and the molecular function of purified recombinant protein was also characterized. The recombinant batroxobin had an apparent molecular weight of 33 kDa by SDS\u2013PAGE analysis and biochemical activities similar to those of native batroxobin. The purified recombinant protein strongly converted fibrinogen into fibrin clot in vitro, and shortened bleeding time and whole blood coagulation time in vivo. However, it did not make any considerable alterations on other blood coagulation factors. Several lines of experimental evidence in this study suggest that the recombinant batroxobin is a potent pro\u2010coagulant agent.<\/jats:p>","DOI":"10.1016\/j.febslet.2004.06.060","type":"journal-article","created":{"date-parts":[[2004,7,7]],"date-time":"2004-07-07T15:49:56Z","timestamp":1089215396000},"page":"67-73","source":"Crossref","is-referenced-by-count":43,"title":["Functional characterization of recombinant batroxobin, a snake venom thrombin\u2010like enzyme, expressed from <i>Pichia pastoris<\/i>"],"prefix":"10.1002","volume":"571","author":[{"given":"Weon-Kyoo","family":"You","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won-Seok","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You-Seok","family":"Koh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang-Cheol","family":"Shin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangsoo","family":"Jang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang-Hoe","family":"Chung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,7,2]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(00)01178-0"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(99)00268-X"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(98)00126-3"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1614965"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1645668"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(76)45021-8"},{"key":"e_1_2_5_8_1","first-page":"3125","volume":"262","author":"Itoh N.","year":"1987","journal-title":"J. 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