{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:31:12Z","timestamp":1791307872803,"version":"4.3.1"},"reference-count":48,"publisher":"Wiley","issue":"1-3","license":[{"start":{"date-parts":[[2004,7,21]],"date-time":"2004-07-21T00:00:00Z","timestamp":1090368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2004,8,13]]},"abstract":"<jats:p>Two novel peptides that inhibit the intra\u2010erythrocyte stage of <jats:italic>Plasmodium falciparum<\/jats:italic> in vitro were identified in the venom of the Trinidad chevron tarantula, <jats:italic>Psalmopoeus cambridgei<\/jats:italic>. Psalmopeotoxin I (PcFK1) is a 33\u2010residue peptide and Psalmopeotoxin II (PcFK2) has 28\u2010amino acid residues; both have three disulfide bridges and belong to the Inhibitor Cystine Knot superfamily. The cDNAs encoding both peptides were cloned, and nucleotide sequence analysis showed that the peptides are synthesized with typical signal peptides and pro\u2010sequences that are cleaved at a basic doublet before secretion of the mature peptides. The IC<jats:sub>5O<\/jats:sub> of PcFK1 for inhibiting <jats:italic>P. falciparum<\/jats:italic> growth was 1.59 \u00b1 1.15 \u03bcM and that of PcFK2 was 1.15 \u00b1 0.95 \u03bcM. PcFK1 was adsorbed strongly to uninfected erythrocytes, but PcFK2 was not. Neither peptide has significant hemolytic activity at 10 \u03bcM. Electrophysiological recordings in isolated frog and mouse neuromuscular preparations revealed that the peptides (at up to 9.3 \u03bcM) do not affect neuromuscular transmission or quantal transmitter release. PcFK1 and PcFK2 do not affect the growth or viability of human epithelial cells, nor do they have any antifungal or antibacterial activity at 20 \u03bcM. Thus, PcFK1 and PcFK2 seem to interact specifically with infected erythrocytes. They could therefore be promising tools for antimalaria research and be the basis for the rational development of antimalarial drugs.<\/jats:p>","DOI":"10.1016\/j.febslet.2004.07.019","type":"journal-article","created":{"date-parts":[[2004,7,22]],"date-time":"2004-07-22T15:34:04Z","timestamp":1090510444000},"page":"109-117","source":"Crossref","is-referenced-by-count":66,"title":["Isolation and characterization of Psalmopeotoxin I and II: two novel antimalarial peptides from the venom of the tarantula <i>Psalmopoeus cambridgei<\/i>"],"prefix":"10.1002","volume":"572","author":[{"given":"Soo-Jin","family":"Choi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romain","family":"Parent","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carole","family":"Guillaume","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christiane","family":"Deregnaucourt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christiane","family":"Delarbre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M","family":"Ojcius","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Jacques","family":"Montagne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Louise","family":"C\u00e9l\u00e9rier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aude","family":"Phelipot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Amiche","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordi","family":"Molgo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Michel","family":"Camadro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Guette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,7,21]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"1353","volume":"40","author":"Pal S.K.","year":"2002","journal-title":"Indian J. 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