{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T07:27:05Z","timestamp":1781162825882,"version":"3.54.1"},"reference-count":22,"publisher":"Wiley","issue":"1-3","license":[{"start":{"date-parts":[[2004,8,18]],"date-time":"2004-08-18T00:00:00Z","timestamp":1092787200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2004,9,10]]},"abstract":"<jats:p>3C\u2010like (3CL) protease is essential for the life cycle of severe acute respiratory syndrome\u2010coronavirus (SARS\u2010CoV) and therefore represents a key anti\u2010viral target. A compound library consisting of 960 commercially available drugs and biologically active substances was screened for inhibition of SARS\u2010CoV 3CL protease. Potent inhibition was achieved using the mercury\u2010containing compounds thimerosal and phenylmercuric acetate, as well as hexachlorophene. As well, 1\u201310 \u03bcM of each compound inhibited viral replication in Vero E6 cell culture. Detailed mechanism studies using a fluorescence\u2010based protease assay demonstrated that the three compounds acted as competitive inhibitors (<jats:italic>K<\/jats:italic>\n<jats:sub>i<\/jats:sub>=0.7, 2.4, and 13.7 \u03bcM for phenylmercuric acetate, thimerosal, and hexachlorophene, respectively). A panel of metal ions including Zn<jats:sup>2+<\/jats:sup> and its conjugates were then evaluated for their anti\u20103CL protease activities. Inhibition was more pronounced using a zinc\u2010conjugated compound (1\u2010hydroxypyridine\u20102\u2010thione zinc; <jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/feb2s0014579304010087-math-si1.gif\" xlink:title=\"urn:x-wiley:00145793:media:feb2s0014579304010087:feb2s0014579304010087-math-si1\"\/>M) than using the ion alone (<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/feb2s0014579304010087-math-si2.gif\" xlink:title=\"urn:x-wiley:00145793:media:feb2s0014579304010087:feb2s0014579304010087-math-si2\"\/>M).<\/jats:p>","DOI":"10.1016\/j.febslet.2004.08.015","type":"journal-article","created":{"date-parts":[[2004,8,19]],"date-time":"2004-08-19T13:10:26Z","timestamp":1092921026000},"page":"116-120","source":"Crossref","is-referenced-by-count":84,"title":["Evaluation of metal\u2010conjugated compounds as inhibitors of 3CL protease of SARS\u2010CoV"],"prefix":"10.1002","volume":"574","author":[{"given":"John T.-A.","family":"Hsu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Jung","family":"Kuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsing-Pang","family":"Hsieh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeau-Ching","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuo-Kuei","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coney P.-C.","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping-Fang","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Po-Huang","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,8,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa030747"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/423240a"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa030781"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)13077-2"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M310875200"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdf327"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1085658"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1835675100"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2004.03.035"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2003.08.066"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi0361766"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.04.098"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0403596101"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3430029"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/248588a0"},{"key":"e_1_2_6_17_1","first-page":"425","volume":"66","author":"Merluzzi V.J.","year":"1989","journal-title":"Res. 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