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Here, we show by fluorescence confocal microscopy on lipid vesicles that the process of hIAPP amyloid formation is accompanied by a loss of barrier function, whereby lipids are extracted from the membrane and taken up in the forming amyloid deposits. No membrane interaction was observed when preformed fibrils were used. It is proposed that lipid uptake from the cell membrane is responsible for amyloid\u2010induced membrane damage and that this represents a general mechanism underlying the cytotoxicity of amyloid forming proteins.<\/jats:p>","DOI":"10.1016\/j.febslet.2004.09.075","type":"journal-article","created":{"date-parts":[[2004,10,13]],"date-time":"2004-10-13T13:53:40Z","timestamp":1097675620000},"page":"117-120","source":"Crossref","is-referenced-by-count":250,"title":["Islet amyloid polypeptide\u2010induced membrane leakage involves uptake of lipids by forming amyloid fibers"],"prefix":"10.1002","volume":"577","author":[{"given":"Emma","family":"Sparr","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maarten F.M.","family":"Engel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitri V.","family":"Sakharov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariette","family":"Sprong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jet","family":"Jacobs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"de Kruijff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo W.M.","family":"H\u00f6ppener","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Antoinette Killian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,10,12]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200008103430607"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02264"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1079469"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2003.09.009"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.48.3.491"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi034889i"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(01)02972-6"},{"key":"e_1_2_5_9_1","unstructured":"Knight J.D. and Miranker A.D. 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