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The activity decreased to below 10% when caspase\u201014 was removed by immunodepletion showing that caspase\u201014 is the predominant caspase in SC. In contrast to normal SC, where caspase\u201014 was present exclusively in its processed form, incompletely matured SC of parakeratotic skin from psoriasis and seborrheic dermatitis contained both procaspase\u201014 and caspase\u201014 subunits. Fractionation of extract from parakeratotic SC revealed that the peak caspase activity coeluted with processed caspase\u201014 but not with procaspase\u201014. Our results suggest that during regular terminal keratinocyte differentiation, endogenous procaspase\u201014 is converted to caspase\u201014 subunits that are catalytically active in the outermost layers of normal human skin.<\/jats:p>","DOI":"10.1016\/j.febslet.2004.10.046","type":"journal-article","created":{"date-parts":[[2004,12,2]],"date-time":"2004-12-02T00:24:18Z","timestamp":1101947058000},"page":"446-450","source":"Crossref","is-referenced-by-count":52,"title":["Stratum corneum\u2010derived caspase\u201014 is catalytically active"],"prefix":"10.1002","volume":"577","author":[{"given":"Heinz","family":"Fischer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Stichenwirth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Dockal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minoo","family":"Ghannadan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Buchberger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juergen","family":"Bach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Kapetanopoulos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wim","family":"Declercq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erwin","family":"Tschachler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leopold","family":"Eckhart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,10,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207107"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.5315"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4400989"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(02)00289-9"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401364"},{"key":"e_1_2_6_7_1","first-page":"5201","article-title":"Identification and characterization of murine caspase-14, a new member of the caspase family","volume":"58","author":"Ahmad M.","year":"1998","journal-title":"Cancer Res"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.45.29648"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4400444"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2000.00205.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.3698"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4400785"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4400897"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401141"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2002.19532.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(02)02015-6"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi0498048"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(03)00051-0"},{"key":"e_1_2_6_19_1","doi-asserted-by":"crossref","unstructured":"Alibardi L. 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