{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T09:21:04Z","timestamp":1770888064036,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2005,2,28]]},"abstract":"<jats:p>Persistent tumour necrosis factor alpha (TNF\u2010\u03b1) exposure uncouples proximal T\u2010cell receptor (TCR)\u2010signalling events. Here, we demonstrate that chronic TNF\u2010\u03b1 exposure also attenuates signalling distal to the TCR, by specifically inhibiting Ca<jats:sup>2+<\/jats:sup> influx evoked by thapsigargin in CD4+ T\u2010cells. Mitogen\u2010induced Ca<jats:sup>2+<\/jats:sup> responses were impaired in a dose dependent manner, and TCR\u2010induced Ca<jats:sup>2+<\/jats:sup> responses were also significantly reduced. The impairment of Ca<jats:sup>2+<\/jats:sup> influx strongly correlated with poor function as proliferative responses to both mitogen and anti\u2010CD3\/CD28 stimulation were suppressed. Our findings show that persistent TNF\u2010\u03b1 exposure of T\u2010cells specifically inhibits store operated Ca<jats:sup>2+<\/jats:sup> influx. This may affect gene activation and contribute to the poor T\u2010cell function in chronic inflammatory disease.<\/jats:p>","DOI":"10.1016\/j.febslet.2005.01.051","type":"journal-article","created":{"date-parts":[[2005,2,8]],"date-time":"2005-02-08T15:14:38Z","timestamp":1107875678000},"page":"1539-1544","source":"Crossref","is-referenced-by-count":10,"title":["Persistent TNF\u2010\u03b1 exposure impairs store operated calcium influx in CD4+ T lymphocytes"],"prefix":"10.1002","volume":"579","author":[{"given":"Leigh D.","family":"Church","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John E.","family":"Goodall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"Rider","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul A.","family":"Bacon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen P.","family":"Young","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar557"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.193.4.427"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.bmb.a072967"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117394"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.185.9.1573"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.9.5495"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.60.2.133"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.156.4.1646"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0308231100"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/86318"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830250612"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1996.d01-768.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200006)43:6<1257::AID-ANR8>3.0.CO;2-Q"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.7.2873"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/386855a0"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(86)90026-6"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0019-2805.2001.01321.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)31639-9"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2003.11.020"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.13.040195.003203"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.24.13795"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(00)01725-4"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(03)00763-0"},{"key":"e_1_2_6_25_1","first-page":"OP165","article-title":"TNF-alpha modulates TCR-induced intracellular calcium signalling in T cells: implications for sphingomyelinase activation","volume":"104","author":"Church L.D.","year":"2001","journal-title":"Immunology"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.febslet.2005.01.051","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.febslet.2005.01.051","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T13:24:49Z","timestamp":1697117089000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/j.febslet.2005.01.051"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,2,4]]},"references-count":24,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2005,2,28]]}},"alternative-id":["10.1016\/j.febslet.2005.01.051"],"URL":"https:\/\/doi.org\/10.1016\/j.febslet.2005.01.051","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,2,4]]}}}