{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T03:23:28Z","timestamp":1791429808362,"version":"4.3.3"},"reference-count":43,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2005,4,14]],"date-time":"2005-04-14T00:00:00Z","timestamp":1113436800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2005,5,9]]},"abstract":"<jats:p>Exposure to limiting oxygen in cells and tissues induce the stabilization and transcriptional activation of the hypoxia\u2010inducible factor 1 alpha (HIF\u20101\u03b1) protein, a key regulator of the hypoxic response. Reactive oxygen species (ROS) generation has been implicated in the stabilization of HIF\u20101\u03b1 during this response, but this is still a matter of some debate. In this study we utilize a mitochondria\u2010targeted antioxidant, mitoubiquinone (MitoQ), and examine its effects on the hypoxic stabilization of HIF\u20101\u03b1. Our results show that under conditions of reduced oxygen (3% O<jats:sub>2<\/jats:sub>), MitoQ ablated the hypoxic induction of ROS generation and destabilized HIF\u20101\u03b1 protein. This in turn led to an abrogation of HIF\u20101 transcriptional activity. Normoxic stabilization of HIF\u20101\u03b1, on the other hand, was unchanged in the presence of MitoQ suggesting that ROS were not involved. This study strongly suggests that mitochondrial ROS contribute to the hypoxic stabilization of HIF\u20101\u03b1.<\/jats:p>","DOI":"10.1016\/j.febslet.2005.03.088","type":"journal-article","created":{"date-parts":[[2005,4,15]],"date-time":"2005-04-15T11:24:43Z","timestamp":1113564283000},"page":"2669-2674","source":"Crossref","is-referenced-by-count":112,"title":["A targeted antioxidant reveals the importance of mitochondrial reactive oxygen species in the hypoxic signaling of HIF\u20101\u03b1"],"prefix":"10.1002","volume":"579","author":[{"given":"Alejandra","family":"Sanju\u00e1n-Pla","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana 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