{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T10:34:20Z","timestamp":1773657260660,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2005,4,9]],"date-time":"2005-04-09T00:00:00Z","timestamp":1113004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2005,5,9]]},"abstract":"<jats:p>\n<jats:italic>Borrelia burgdorferi<\/jats:italic> and other spirochetes contain a class I lysyl\u2010tRNA synthetase (LysRS), in contrast to most eubacteria that have a canonical class II LysRS. We analyzed tRNA<jats:sup>Lys<\/jats:sup> recognition by <jats:italic>B. burgdorferi<\/jats:italic> LysRS, using two complementary approaches. First, the nucleotides of <jats:italic>B. burgdorferi<\/jats:italic> tRNA<jats:sup>Lys<\/jats:sup> in contact with <jats:italic>B. burgdorferi<\/jats:italic> LysRS were determined by enzymatic footprinting experiments. Second, the kinetic parameters for a series of variants of the <jats:italic>B. burgdorferi<\/jats:italic> tRNA<jats:sup>Lys<\/jats:sup> were then determined during aminoacylation by <jats:italic>B. burgdorferi<\/jats:italic> LysRS. The identity elements were found to be mostly located in the anticodon and in the acceptor stem. Transplantation of the identified identity elements into the <jats:italic>Escherichia coli<\/jats:italic> tRNA<jats:sup>Asp<\/jats:sup> scaffold endowed lysylation activity on the resulting chimera, indicating that a functional <jats:italic>B. burgdorferi<\/jats:italic> lysine tRNA identity set had been determined.<\/jats:p>","DOI":"10.1016\/j.febslet.2005.04.001","type":"journal-article","created":{"date-parts":[[2005,4,10]],"date-time":"2005-04-10T11:08:30Z","timestamp":1113131310000},"page":"2629-2634","source":"Crossref","is-referenced-by-count":9,"title":["Transfer RNA recognition by class I lysyl\u2010tRNA synthetase from the Lyme disease pathogen <i>Borrelia burgdorferi<\/i>"],"prefix":"10.1002","volume":"579","author":[{"given":"Alexandre","family":"Ambrogelly","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magali","family":"Frugier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Ibba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dieter","family":"S\u00f6ll","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Gieg\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/embo-reports\/kve095"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5446.1893"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.69.1.617"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/26.22.5017"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0282(1999)52:1<1::AID-BIP1>3.0.CO;2-W"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.278.5340.1119"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.26.14383"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.26.14224"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.16.4594-4600.2002"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.2.418"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/20.9.2335"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80258-X"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.23.9260"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb01022.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1998.1711"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/15.21.8783"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)01096-5"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.19.11295"},{"key":"e_1_2_6_20_1","unstructured":"2003 Landes Biosceinces Kluwer Academic\/Plenum Publishers New York R. 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