{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:24:23Z","timestamp":1791271463081,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2005,5,11]],"date-time":"2005-05-11T00:00:00Z","timestamp":1115769600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2005,6,6]]},"abstract":"<jats:p>Somatostatin (SST) peptide is a potent inhibitor of insulin secretion and its effect is mediated via somatostatin receptor 5 (SSTR5) in the endocrine pancreas. To investigate the consequences of gene ablation of SSTR5 in the mouse pancreas, we have generated a mouse model in which the SSTR5 gene was specifically knocked down in the pancreatic beta cells (\u03b2SSTR5<jats:sup>Kd<\/jats:sup>) using the Cre\u2010lox system. Immunohistochemistry analysis showed that SSTR5 gene expression was absent in beta cells at three months of age. At the time of gene ablation, \u03b2SSTR5<jats:sup>Kd<\/jats:sup> mice demonstrated glucose intolerance with lack of insulin response and significantly reduced serum insulin levels. Insulin tolerance test demonstrated a significant increase of insulin clearance in vivo at the same age. In vitro studies demonstrated an absence of response to SST\u201028 stimulation in the \u03b2SSTR5<jats:sup>Kd<\/jats:sup> mouse islet, which was associated with a significantly reduced SST expression level in \u03b2SSTR5<jats:sup>Kd<\/jats:sup> mice pancreata. In addition, \u03b2SSTR5<jats:sup>Kd<\/jats:sup> mice had significantly reduced serum glucose levels and increased serum insulin levels at 12 months of age. Glucose tolerance test at an older age also indicated a persistently higher insulin level in \u03b2SSTR5<jats:sup>Kd<\/jats:sup> mice. Further studies of \u03b2SSTR5<jats:sup>Kd<\/jats:sup> mice had revealed elevated serum C\u2010peptide levels at both 3 and 12 months of age, suggesting that these mice are capable of producing and releasing insulin to the periphery. These results support the hypothesis that SSTR5 plays a pivotal role in the regulation of insulin secretion in the mouse pancreas.<\/jats:p>","DOI":"10.1016\/j.febslet.2005.04.069","type":"journal-article","created":{"date-parts":[[2005,5,12]],"date-time":"2005-05-12T11:44:59Z","timestamp":1115898299000},"page":"3107-3114","source":"Crossref","is-referenced-by-count":37,"title":["SSTR5 ablation in islet results in alterations in glucose homeostasis in mice"],"prefix":"10.1002","volume":"579","author":[{"given":"X.P.","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.A.","family":"Norman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Magnusson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.J.","family":"DeMayo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.C.","family":"Brunicardi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,5,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-109-6-1943"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.179.4068.77"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(20)81928-0"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)35416-X"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.1.251"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)88719-7"},{"key":"e_1_2_6_8_1","first-page":"838","article-title":"Clone somatostatin receptors: identification of subtype-selective peptides and demonstration of high affinity binding of linear peptides","volume":"43","author":"Raynor K.","year":"1993","journal-title":"Mol. 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