{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T12:15:46Z","timestamp":1791807346649,"version":"4.3.4"},"reference-count":40,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2005,6,8]],"date-time":"2005-06-08T00:00:00Z","timestamp":1118188800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2005,7,4]]},"abstract":"<jats:p>Several anti\u2010angiogenic factors are derived from proteolytic processing of large molecules including endostatin from type XVIII collagen and angiostatin from plasminogen. In previous studies we showed that neostatin\u20107, the C\u2010terminal 28 kDa endostatin\u2010spanning proteolytic fragment, is generated from the proteolytic action of matrix metalloproteinase matrilysin (MMP)\u20107 on type XVIII collagen. Now, we report a second member of the neostatin family of proteins, neostatin\u201014. Given the small quantities of neostatin\u20107 and \u201014 generated by the breakdown of naturally occurring collagen XVIII (using MMP\u20107 and \u201014, respectively), we used two other approaches to characterize the anti\u2010angiogenic properties of these molecules: murine recombinant neostatin in vitro, and gene therapy. We demonstrate that murine recombinant neostatin\u20107 inhibits calf pulmonary artery endothelial cell proliferation and that microinjection of neostatin\u20107 and neostatin\u201014 naked DNA into the corneal stroma of mice results in significant reduction of basic fibroblast growth factor\u2010induced corneal neovascularization. These results provide supportive evidence of the possible anti\u2010angiogenic effect of neostatins.<\/jats:p>","DOI":"10.1016\/j.febslet.2005.05.043","type":"journal-article","created":{"date-parts":[[2005,6,10]],"date-time":"2005-06-10T15:47:16Z","timestamp":1118418436000},"page":"3601-3606","source":"Crossref","is-referenced-by-count":69,"title":["Functional characterization of neostatins, the MMP\u2010derived, enzymatic cleavage products of type XVIII collagen"],"prefix":"10.1002","volume":"579","author":[{"given":"Jin-Hong","family":"Chang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel A.D.","family":"Javier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gene-Yuan","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailton B.","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitri T.","family":"Azar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,6,8]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.39.25404"},{"key":"e_1_2_5_3_1","first-page":"2520","article-title":"Anti-angiogenic cues from vascular basement membrane collagen","volume":"60","author":"Colorado P.C.","year":"2000","journal-title":"Cancer Res."},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M209276200"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M206358200"},{"key":"e_1_2_5_6_1","first-page":"2517","article-title":"Matrilysin cleavage of corneal collagen type XVIII NC1 domain and generation of a 28-kDa fragment","volume":"42","author":"Lin H.C.","year":"2001","journal-title":"Invest. 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