{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:17:19Z","timestamp":1770823039375,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2005,6,8]],"date-time":"2005-06-08T00:00:00Z","timestamp":1118188800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2005,7,4]]},"abstract":"<jats:p>The phytochemical 11,11\u2032\u2010dideoxyverticillin, derived from the fungus <jats:italic>Shiraia bambusicola<\/jats:italic>, has been shown to possess potent anticancer activity in vitro and in vivo. Here, we investigated the effect of 11,11\u2032\u2010dideoxyverticillin on cell cycle progression, and explored the potential mechanisms for this effect. A concentration\u2010 and time\u2010dependent cell cycle blockade at G<jats:sub>2<\/jats:sub>\/M phase was observed in human colon cancer cells (HCT\u2010116) following 11,11\u2032\u2010dideoxyverticillin treatment and was associated with marked increases in levels of p53, phospho\u2010p53(ser20) and phospho\u2010Chk2(Thr 68). When wild type p53 expression was specifically inhibited by RNA interference, HCT\u2010116 cells treated with 11,11\u2032\u2010dideoxyverticillin failed to arrest in G<jats:sub>2<\/jats:sub>\/M and did not show increased phospho\u2010Chk2(Thr 68). On the other hand, 11,11\u2032\u2010dideoxyverticillin treatment also elicited p38 MAP kinase activity and expression of phospho\u2010p38 MAPK. Treatment with a specific p38 MAPK inhibitor (SB203580) successfully inhibited p38 MAPK and delayed the onset of G<jats:sub>2<\/jats:sub>\/M arrest induced by 0.5 \u03bcM 11,11\u2032\u2010dideoxyverticillin after approximately 6 h, but did not abolish the induction of G<jats:sub>2<\/jats:sub>\/M arrest. Additionally, SB203580 did not alter the levels of p53, phospho\u2010p53 (ser20), or phospho\u2010Chk2 (Thr68) proteins in 11,11\u2032\u2010dideoxyverticillin\u2010treated cells. Together, these findings indicate that p53\u2010mediated phosphorylation of Chk2 maybe plays a vital role in 11,11\u2032\u2010dideoxyverticillin\u2010induced G<jats:sub>2<\/jats:sub>\/M arrest, and that p38 MAPK might accelerate this progression. Our work suggests a new possibility of interactions among p53, Chk2 and p38 MAPK signaling in G<jats:sub>2<\/jats:sub>\/M arrest.<\/jats:p>","DOI":"10.1016\/j.febslet.2005.05.053","type":"journal-article","created":{"date-parts":[[2005,6,10]],"date-time":"2005-06-10T15:47:16Z","timestamp":1118418436000},"page":"3683-3690","source":"Crossref","is-referenced-by-count":21,"title":["The p53 pathway is synergized by p38 MAPK signaling to mediate 11,11\u2032\u2010dideoxyverticillin\u2010induced G<sub>2<\/sub>\/M arrest"],"prefix":"10.1002","volume":"579","author":[{"given":"Yi","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ze-Hong","family":"Miao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Min","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Ding","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.10.2.8641557"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00690209"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0955-0674(94)90059-0"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(00)01773-6"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.02.115"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001813-200506000-00007"},{"key":"e_1_2_6_8_1","first-page":"230","article-title":"The potential of soybean foods as a chemoprevention approach for human urinary tract cancer","volume":"6","author":"Su S.J.","year":"2000","journal-title":"Clin. 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