{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T03:14:43Z","timestamp":1761016483163},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,1,4]],"date-time":"2006-01-04T00:00:00Z","timestamp":1136332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2006,1,23]]},"abstract":"<jats:p>The first example of a new sub\u2010family of toxins (\u03b1\u2010KTx20.1) from the scorpion <jats:italic>Tityus trivittatus<\/jats:italic> was purified, sequenced and characterized physiologically. It has 29 amino acid residues, three disulfide bridges assumed to adopt the cysteine\u2010stabilized \u03b1\/\u03b2 scaffold with a p<jats:italic>I<\/jats:italic> value of 8.98. The sequence identities with all the other known \u03b1\u2010KTx are less than 40%. Its effects were verified using seven different cloned K<jats:sup>+<\/jats:sup> channels (vertebrate Kv1.1\u20131.5, <jats:italic>Shaker<\/jats:italic> IR and hERG) expressed in <jats:italic>Xenopus leavis<\/jats:italic> oocytes. The toxin\u2010induced effects show large differences among the different K<jats:sup>+<\/jats:sup> channels and a preference towards Kv1.3 (EC50 = 7.9 \u00b1 1.4 nM).<\/jats:p>","DOI":"10.1016\/j.febslet.2005.12.073","type":"journal-article","created":{"date-parts":[[2006,1,5]],"date-time":"2006-01-05T13:25:35Z","timestamp":1136467535000},"page":"592-596","source":"Crossref","is-referenced-by-count":28,"title":["A novel toxin from the venom of the scorpion <i>Tityus trivittatus<\/i>, is the first member of a new \u03b1\u2010KTX subfamily"],"prefix":"10.1002","volume":"580","author":[{"given":"Yousra","family":"Abdel-Mottaleb","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fredy V.","family":"Coronas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adolfo R.","family":"de Roodt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lourival D.","family":"Possani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Tytgat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,1,4]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_5_2_1","DOI":"10.1016\/S0041-0101(96)00198-5"},{"doi-asserted-by":"publisher","key":"e_1_2_5_3_1","DOI":"10.1016\/S0041-0101(02)00247-7"},{"doi-asserted-by":"publisher","key":"e_1_2_5_4_1","DOI":"10.1016\/j.toxicon.2004.03.022"},{"doi-asserted-by":"publisher","key":"e_1_2_5_5_1","DOI":"10.1111\/j.1749-6632.1999.tb11293.x"},{"doi-asserted-by":"publisher","key":"e_1_2_5_6_1","DOI":"10.1016\/S0165-6147(99)01398-X"},{"doi-asserted-by":"publisher","key":"e_1_2_5_7_1","DOI":"10.1016\/S0041-0101(01)00252-5"},{"doi-asserted-by":"publisher","key":"e_1_2_5_8_1","DOI":"10.1093\/nar\/25.17.3389"},{"doi-asserted-by":"publisher","key":"e_1_2_5_9_1","DOI":"10.1073\/pnas.85.8.2444"},{"doi-asserted-by":"publisher","key":"e_1_2_5_10_1","DOI":"10.1093\/nar\/gkg500"},{"doi-asserted-by":"publisher","key":"e_1_2_5_11_1","DOI":"10.1042\/bj20031324"},{"doi-asserted-by":"publisher","key":"e_1_2_5_12_1","DOI":"10.1016\/0014-5793(88)81015-9"},{"doi-asserted-by":"publisher","key":"e_1_2_5_13_1","DOI":"10.1042\/bj20031860"},{"doi-asserted-by":"publisher","key":"e_1_2_5_14_1","DOI":"10.1038\/313316a0"},{"doi-asserted-by":"publisher","key":"e_1_2_5_15_1","DOI":"10.1085\/jgp.93.6.1061"},{"doi-asserted-by":"publisher","key":"e_1_2_5_16_1","DOI":"10.1006\/bbrc.2000.3756"},{"doi-asserted-by":"publisher","key":"e_1_2_5_17_1","DOI":"10.1074\/jbc.272.7.4302"},{"doi-asserted-by":"publisher","key":"e_1_2_5_18_1","DOI":"10.1016\/S1570-9639(02)00458-2"},{"doi-asserted-by":"publisher","key":"e_1_2_5_19_1","DOI":"10.1016\/S0014-5793(03)00125-X"},{"doi-asserted-by":"publisher","key":"e_1_2_5_20_1","DOI":"10.1016\/S0167-0115(00)00116-6"},{"doi-asserted-by":"publisher","key":"e_1_2_5_21_1","DOI":"10.1042\/bj20030115"},{"doi-asserted-by":"publisher","key":"e_1_2_5_22_1","DOI":"10.1146\/annurev.immunol.22.012703.104702"},{"doi-asserted-by":"publisher","key":"e_1_2_5_23_1","DOI":"10.1172\/JCI16921"},{"doi-asserted-by":"publisher","key":"e_1_2_5_24_1","DOI":"10.1016\/j.tips.2004.03.010"},{"doi-asserted-by":"publisher","key":"e_1_2_5_25_1","DOI":"10.1042\/BJ20041379"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.febslet.2005.12.073","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.febslet.2005.12.073","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T09:55:17Z","timestamp":1697104517000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/j.febslet.2005.12.073"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,1,4]]},"references-count":24,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2006,1,23]]}},"alternative-id":["10.1016\/j.febslet.2005.12.073"],"URL":"https:\/\/doi.org\/10.1016\/j.febslet.2005.12.073","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"type":"print","value":"0014-5793"},{"type":"electronic","value":"1873-3468"}],"subject":[],"published":{"date-parts":[[2006,1,4]]}}}