{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:14:17Z","timestamp":1791191657297,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2006,2,24]],"date-time":"2006-02-24T00:00:00Z","timestamp":1140739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2006,3,20]]},"abstract":"<jats:p>Flavohemoglobins and flavodiiron proteins are two families of enzymes involved in nitrosative detoxification. However, the physiological oxygen\u2010related conditions under which they work and their relative role are still a matter of debate. To address this question we analyzed the function of the putative flavohemoprotein of <jats:italic>Staphylococcus aureus<\/jats:italic>, an organism that lacks a flavodiiron\u2010like gene. In this report we show that the recombinant protein contains all features typical of canonical flavohemoglobins and that the transcription of flavohemoglobin gene was upregulated by nitrosative stress in an oxygen\u2010dependent manner. However, and in contrast to other bacterial flavohemoglobins, the <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> protein has no apparent role in aerobic nitrosative protection, being only beneficial when cells of <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> are submitted to nitrosative stress in a microaerophilic environment. The in vivo data corroborates the proposal that Hmp acts physiologically as a denitrosylase.<\/jats:p>","DOI":"10.1016\/j.febslet.2006.02.039","type":"journal-article","created":{"date-parts":[[2006,2,25]],"date-time":"2006-02-25T12:16:10Z","timestamp":1140869770000},"page":"1817-1821","source":"Crossref","is-referenced-by-count":51,"title":["Flavohemoglobin requires microaerophilic conditions for nitrosative protection of <i>Staphylococcus aureus<\/i>"],"prefix":"10.1002","volume":"580","author":[{"given":"Vera L.","family":"Gon\u00e7alves","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00edgia S.","family":"Nobre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o B.","family":"Vicente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Teixeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00edgia M.","family":"Saraiva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,2,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2911(04)49002-X"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-6445(03)00056-1"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.24.14100"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.181199698"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.18.10378"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)00157-X"},{"key":"e_1_2_6_8_1","unstructured":"Smith P.K. Krohn R.J. Hermanson G.T. Mallia A.K. Gartner F.H. Provenzano M.D. Fujimoto E.K. Gorke N.M. Olson B.J. and Klenk D.C. (2004) Bacterial hemoglobins: old proteins with new functions? Roles in respiratory and nitric oxide metabolism in: Respiration in Archaea and Bacteria Diversity of Prokaryotic Electron Transport Carriers (Davide Zanoni Ed.) pp. 251\u2013284."},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110470200"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.090083597"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M411070200"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199808203390806"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI18535"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.4.1074-1079.2000"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02026129"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(02)00481-X"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.1996.2.331"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1998.00312.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)36879-6"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90643-9"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi001844y"},{"key":"e_1_2_6_22_1","first-page":"3","volume-title":"Microbial Determinants of Virulence and Host Response","author":"Novick R.","year":"1990"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02199440"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2004.10.003"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.4.1113-1123.2001"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.9.5205-5209.2000"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.febslet.2006.02.039","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.febslet.2006.02.039","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T03:57:36Z","timestamp":1697169456000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/j.febslet.2006.02.039"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,2,24]]},"references-count":25,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2006,3,20]]}},"alternative-id":["10.1016\/j.febslet.2006.02.039"],"URL":"https:\/\/doi.org\/10.1016\/j.febslet.2006.02.039","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,2,24]]}}}