{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T16:53:24Z","timestamp":1758128004397},"reference-count":30,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2006,5,8]],"date-time":"2006-05-08T00:00:00Z","timestamp":1147046400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2006,5,29]]},"abstract":"<jats:p>Defects of cell\u2013cell adhesion underlie disruption of epithelial integrity observed in patients with pemphigus vulgaris (PV), an autoimmune disease characterized by severe mucosal erosions and skin blisters. Pathogenic PV autoantibodies found in patients\u2019 sera target desmoglein 3 (Dsg3), a major component of the desmosome, but how does this phenomenon affect Dsg\u2010dependent adhesion and lead to acantholysis still remains controversial. Here, we show that PV serum determines a reduction of Dsg3 half\u2010life in HaCaT keratinocytes, although the total amount of Dsg3 remains unchanged. Immunofluorescence studies suggest that PV IgG exert their effect prevalently by binding non\u2010desmosomal Dsg3 without causing its massive internalization. Furthermore, PV IgG targeting desmosome\u2010assembled Dsg3 do not induce depletion of Dsg3 from the adhesion sites. Conversely, incorporation of PV IgG\u2010Dsg3 complexes into new forming desmosomes appears perturbed. With our study, the basic biochemical changes of Dsg3 in an in vitro model of PV have been defined.<\/jats:p>","DOI":"10.1016\/j.febslet.2006.04.089","type":"journal-article","created":{"date-parts":[[2006,5,9]],"date-time":"2006-05-09T11:13:20Z","timestamp":1147173200000},"page":"3276-3281","source":"Crossref","is-referenced-by-count":38,"title":["Serum from pemphigus vulgaris reduces desmoglein 3 half\u2010life and perturbs its de novo assembly to desmosomal sites in cultured keratinocytes"],"prefix":"10.1002","volume":"580","author":[{"given":"Nicola","family":"Cirillo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felice","family":"Femiano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Gombos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Lanza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,5,8]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90360-B"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2006.00391.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"crossref","unstructured":"Lanza A. 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