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In order to determine the effects of CBD on sleep, it was administered intracerebroventricular (icv) in a dose of 10 \u03bcg\/5 \u03bcl at the beginning of either the lights\u2010on or the lights\u2010off period. We found that CBD administered during the lights\u2010on period increased wakefulness (W) and decreased rapid eye movement sleep (REMS). No changes on sleep were observed during the dark phase. Icv injections of CBD (10 \u03bcg\/5 \u03bcl) induced an enhancement of <jats:italic>c<\/jats:italic>\u2010Fos expression in waking\u2010related brain areas such as hypothalamus and dorsal raphe nucleus (DRD). Microdialysis in unanesthetized rats was carried out to characterize the effects of icv administration of CBD (10 \u03bcg\/5 \u03bcl) on extracellular levels of dopamine (DA) within the nucleus accumbens. CBD induced an increase in DA release. Finally, in order to test if the waking properties of CBD could be blocked by the sleep\u2010inducing endocannabinoid anandamide (ANA), animals received ANA (10 \u03bcg\/2.5 \u03bcl, icv) followed 15 min later by CBD (10 \u03bcg\/2.5 \u03bcl). Results showed that the waking properties of CBD were not blocked by ANA. In conclusion, we found that CBD modulates waking via activation of neurons in the hypothalamus and DRD. Both regions are apparently involved in the generation of alertness. Also, CBD increases DA levels as measured by microdialysis and HPLC procedures. 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