{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T05:50:46Z","timestamp":1697176246909},"reference-count":36,"publisher":"Wiley","issue":"28-29","license":[{"start":{"date-parts":[[2006,11,14]],"date-time":"2006-11-14T00:00:00Z","timestamp":1163462400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2006,12,11]]},"abstract":"<jats:p>Epstein\u2010Barr virus (EBV) is an omnipresent human virus causing infectious mononucleosis and EBV associated cancers. Its protease is a possible target for antiviral therapy. We studied its dimerization and enzyme kinetics with two enzyme assays based either on the release of paranitroaniline or 7\u2010amino\u20104\u2010methylcoumarin from labeled pentapeptide (Ac\u2010KLVQA) substrates. The protease is in a monomer\u2013dimer equilibrium where only dimers are active. In absence of citrate the <jats:italic>K<\/jats:italic>\n<jats:sub>d<\/jats:sub> is 20 \u03bcM and drops to 0.2 \u03bcM in presence of 0.5 M citrate. Citrate increases additionally the activity of the catalytic sites. The inhibitory constants of different substrate derived peptides and \u03b1\u2010keto\u2010amide based inhibitors, which have at best a <jats:italic>K<\/jats:italic>\n<jats:sub>i<\/jats:sub> of 4 \u03bcM, have also been evaluated.<\/jats:p>","DOI":"10.1016\/j.febslet.2006.11.006","type":"journal-article","created":{"date-parts":[[2006,11,16]],"date-time":"2006-11-16T02:28:15Z","timestamp":1163644095000},"page":"6570-6578","source":"Crossref","is-referenced-by-count":5,"title":["Kinetics, inhibition and oligomerization of Epstein\u2010Barr virus protease"],"prefix":"10.1002","volume":"580","author":[{"given":"Marlyse","family":"Buisson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucie","family":"Rivail","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Fran\u00e7ois","family":"Hernandez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Jamin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean","family":"Martinez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rob W.H.","family":"Ruigrok","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wim P.","family":"Burmeister","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,11,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-0670-3"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1086\/428097"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2002.03764.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.65.10.5149-5156.1991"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.13.7445"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi952842u"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.12.7732"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/383279a0"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/383275a0"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80157-9"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi9712697"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.7.2874"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi001019h"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(02)01040-9"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/095632020001100101"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm970104t"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi027045s"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.12.6657-6665.2004"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.50.30168"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.39.22697"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)32221-4"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi9726077"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi011753g"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.4854"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(67)80055-X"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-1983-30471"},{"key":"e_1_2_6_28_1","first-page":"67","article-title":"XOP: A graphical user interface for spectral calculations and X-ray optics utilities","author":"D&eacute;jus R.","year":"1996","journal-title":"Rev. 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